Patents Assigned to Third Wave Technologies, Inc.
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Patent number: 6214545Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: May 5, 1997Date of Patent: April 10, 2001Assignee: Third Wave Technologies, IncInventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, James E. Dahlberg, Lance Fors
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Patent number: 6210880Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: September 19, 1997Date of Patent: April 3, 2001Assignee: Third Wave Technologies, Inc.Inventors: Victor I. Lyamichev, Fang Dong, Mary Ann D. Brow, Lance Fors, Bruce P. Neri
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Patent number: 6194149Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: March 3, 1998Date of Patent: February 27, 2001Assignee: Third Wave Technologies, Inc.Inventors: Bruce Neri, Fang Dong, Victor Lyamichev, Mary Ann D. Brow, Lance Fors
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Patent number: 6090606Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostabe organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: December 2, 1996Date of Patent: July 18, 2000Assignee: Third Wave Technologies, Inc.Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamicheva
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Patent number: 6090543Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: December 2, 1996Date of Patent: July 18, 2000Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 6001567Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.Type: GrantFiled: July 12, 1996Date of Patent: December 14, 1999Assignee: Third Wave Technologies, Inc.Inventors: Mary Ann D. Brow, Jeff Steven Grotelueschen Hall, Victor Lyamichev, David Michael Olive, James Robert Prudent
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Patent number: 5994069Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.Type: GrantFiled: March 24, 1997Date of Patent: November 30, 1999Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
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Patent number: 5985557Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: November 26, 1996Date of Patent: November 16, 1999Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 5888780Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in nucleic acids. Methods are provided which allow for the identification of genetic mutations and the identification bacterial and viral strains and species in a sample.Type: GrantFiled: February 19, 1997Date of Patent: March 30, 1999Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow, Mary C. Oldenburg
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Patent number: 5846717Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: January 24, 1996Date of Patent: December 8, 1998Assignee: Third Wave Technologies, Inc.Inventors: Mary Ann D. Brow, Jeff Steven Grotelueschen Hall, Victor Lyamichev, David Michael Olive, James Robert Prudent
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Patent number: 5843669Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Structure-specific nucleases, including 5' nucleases, thermostable FEN-1 endonucleases and 3' exonucleases, are used to detect and identify target nucleic acids. Methods are provided which allow for the detection specific nucleic acid sequences; these methods permit the detection and identification of mutant and wild-type forms of genes (e.g., human genes) as well as permit the detection and identification of bacterial and viral pathogens in a sample.Type: GrantFiled: November 29, 1996Date of Patent: December 1, 1998Assignee: Third Wave Technologies, Inc.Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamichev
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Patent number: 5843654Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in the human p53 gene. Methods are provided which allow for the identification of genetic mutations in the human p53 gene in a sample.Type: GrantFiled: June 7, 1995Date of Patent: December 1, 1998Assignee: Third Wave Technologies, Inc.Inventors: Laura M. Heisler, Lance Fors, Mary Ann D. Brow
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Patent number: 5837450Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.Type: GrantFiled: June 6, 1995Date of Patent: November 17, 1998Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
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Patent number: 5795763Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.Type: GrantFiled: June 6, 1995Date of Patent: August 18, 1998Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
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Patent number: 5719028Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.Type: GrantFiled: February 6, 1997Date of Patent: February 17, 1998Assignee: Third Wave Technologies Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow, Mary C. Oldenburg
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Patent number: 5614402Abstract: A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.Type: GrantFiled: June 6, 1994Date of Patent: March 25, 1997Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
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Patent number: 5541311Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.Type: GrantFiled: June 4, 1993Date of Patent: July 30, 1996Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow