Abstract: A method includes capturing an image of a powder bed with a visible light camera that shares an optical axis with a laser beam. The laser beam is configured for heating the powder bed. The method also includes increasing first intensities of first pixels of the image that are indicative of foreign objects within the image and normalizing second intensities of second pixels of the image that are indicative of uneven illumination of the powder bed. The method also includes processing the image via contrast limited adaptive histogram equalization after increasing the first intensities and after normalizing the second intensities. The method also includes using graph-based image segmentation to identify third pixels of the image that correspond to an area of the powder bed that has been processed by the laser beam and indicating the third pixels via a user interface.

Abstract: Processes for alkylating benzene are provided. In embodiments, the process comprises combining benzene, an olefin, and a catalyst composition under conditions to react benzene with the olefin to produce an alkylbenzene, the catalyst composition comprising components selected from the group consisting of an ionic liquid, an acid, and an aromatic; an acid, a base capable of forming an ionic liquid with the acid, and an aromatic; an ionic liquid and an acid; and an acid and a base capable of forming an ionic liquid with the acid. The ionic liquid does not comprise a metal halide and the catalyst composition is free of a metal halide and the aromatic, if present in the catalyst composition, is not the benzene being alkylated.

Abstract: A polyacrylamide-based copolymer reduces or prevents aggregation of biologic molecules including proteins, peptides, and nucleic acids, and lipid-based vehicles such as liposomes, lipid nanoparticles, polymerosomes, and micelles, in aqueous formulations at hydrophobic interfaces, thereby increasing the thermal stability of the molecules in the formulation. Methods and compositions comprising the copolymer and a protein or the copolymer and insulin can be used for treating conditions including diabetes.

Abstract: Provided herein is a pharmaceutical composition comprising an effective amount of cypate-Cyclo(Cys-Gly-Arg-Asp-Ser-Pro-Cys)-Lys-OH (LS301), cypate-Cyclo(Cys-Gly-Arg-Asp-Ser-Pro-Cys)-Tyr-OH (LS838) or pharmaceutically acceptable salts thereof, wherein each amino acid residue is independently in a D or L configuration; a divalent metal ion; and a pharmaceutically acceptable carrier. Further provided are lyophilized products comprising a dye-conjugate and m methods for identifying compromised and for binding phosphorylated annexin A2 (pANXA2) protein in a biological sample using a composition described herein.

Abstract: Disclosed herein, are nanoparticles comprising one or more immune-tolerant elastin-like polypeptide tetramers and one or more immune-tolerant elastin-like fusion molecules. Also, disclosed herein are pharmaceutical compositions including the nanoparticles; methods of administering the nanoparticles to patients for the treatment of cancer; and methods of making the nanoparticles.

Abstract: A polyacrylamide-based copolymer reduces or prevents aggregation of biologic molecules including proteins, peptides, and nucleic acids, and lipid-based vehicles such as liposomes, lipid nanoparticles, polymerosomes, and micelles, in aqueous formulations at hydrophobic interfaces, thereby increasing the thermal stability of the molecules in the formulation. Methods and compositions comprising the copolymer and a protein or the copolymer and insulin can be used for treating conditions including diabetes.

Abstract: A memory cell array of an SRAM including: a top memory cell array including top memory cells; and a bottom memory cell array including bottom memory cells, the top memory cells include: a first top memory cell between a power supply voltage and a middle node, and connected to a first top wordline, a first top bitline and a first top complementary bitline, the bottom memory cells include: a first bottom memory cell to operate with the first top memory cell, connected between the middle node and a ground voltage, and connected to a first bottom wordline, a first bottom bitline and a first bottom complementary bitline, and when write and read operations are not performed on the first top and bottom memory cells, the first top bitline, the first top complementary bitline, the first bottom bitline and the first bottom complementary bitline are connected to the middle node.

Abstract: Disclosed is a system and method for a microdevice to separate blood cells based on differences in antigen expression. Specifically, cells of the same phenotype are separated based on whether or not they are activated during infection or resting. The device of the present disclosure takes a small sample of blood and provides differential cell counts that can be used to test for infection and inflammatory response. The device can be used to identify sepsis and other infections rapidly. By measuring differences in activated white cell counts such as neutrophils, the device of the present disclosure measures physiological response to infection in hospitalized patients recovering from burns, surgeries, etc.

Abstract: A polyacrylamide-based copolymer reduces or prevents aggregation of biologic molecules including proteins, peptides, and nucleic acids, and lipid-based vehicles such as liposomes, lipid nanoparticles, polymerosomes, and micelles, in aqueous formulations at hydrophobic interfaces, thereby increasing the thermal stability of the molecules in the formulation. Methods and compositions comprising the copolymer and a protein or the copolymer and insulin can be used for treating conditions including diabetes.

Abstract: Certain disclosed embodiments concern a bioremediation composition comprising microbial cells, at least one co-metabolism substrate to induce selected enzyme production by the microbial cells, and a bead or gel encapsulating the microbial cells, such as bacterial or fungi cells, and the at least one co-metabolism substrate. For certain embodiments, the substrate is a slow release compound, such as an orthosilicate that hydrolyzes to produce an alcohol growth substrate. Embodiments of a method for using the composition to transform contaminants of concern also are disclosed.

Abstract: Provided herein is a macroscope comprising an objective apparatus comprising a multifocal widefield optics comprising a plurality of optical components configured to focus on a plurality of planes. Also provided herein are methods for analyzing a three-dimensional specimen, the method comprising obtaining, via a macroscope, synchronous multifocal optical images of a plurality of planes of the three-dimensional specimen, wherein the macroscope comprises an objective apparatus comprising a multifocal widefield optics comprising a plurality of optical components configured to focus on a plurality of planes. The three-dimensional specimen can be a biological specimen, such as brain.

Abstract: Systems and methods described herein relate to reconstructing a scene in three dimensions from a two-dimensional image. One embodiment processes an image using a detection transformer to detect an object in the scene and to generate a NOCS map of the object and a background depth map; uses MLPs to relate the object to a differentiable database of object priors (PriorDB); recovers, from the NOCS map, a partial 3D object shape; estimates an initial object pose; fits a PriorDB object prior to align in geometry and appearance with the partial 3D shape to produce a complete shape and refines the initial pose estimate; generates an editable and re-renderable 3D scene reconstruction based, at least in part, on the complete shape, the refined pose estimate, and the depth map; and controls the operation of a robot based, at least in part, on the editable and re-renderable 3D scene reconstruction.

Abstract: Synthetic antimicrobial peptides, compositions comprising thereof, and methods of use for modulating one or more symptoms of an infection in a subject are disclosed. In some aspects, the infection is caused by mycobacteria, for example, a nontuberculous mycobacterium such as Mycobacterium abscessus. In other aspects, the infection is caused by Escherichia coli, Pseudomonas aeruginosa, or methicillin-resistant Staphylococcus aureus (MRSA). Also disclosed are methods of identifying synthetic antimicrobial peptides against a pathogen with no known effective treatment using a library of synthetic peptides.

Abstract: The present disclosure provides a cuboid magnetometer with high fluorescence collection efficiency, used for cardiac magnetic field measurement, and based on nitrogen-vacancy (NV) centers in diamond, and a cardiac magnetic field measurement system. The cardiac magnetic field measurement system includes a magnetic shielding chamber, a non-magnetic bed, an array probe for cardiac magnetic field measurement, a three-axis displacement platform, a high-speed data collection card, a fiber laser source, a microwave source, and a computer. The array probe for cardiac magnetic field measurement includes a non-magnetic shell, a stepping motor, a porous rotary fiber coupling apparatus, a fiber bundle set, a special photodetector (PD) set, a filter set, a ring-shaped antenna, and special diamond.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A flow battery system includes a first tank including a hydrogen reactant, a second tank including a bromine electrolyte, and at least one cell including a first electrolyte side operably connected to the first tank and a second electrolyte side operably connected to the second tank. The battery system further includes a direct connection line directly connecting the first tank and the second tank and configured such that the hydrogen reactant passes between the first tank and the second tank.

Abstract: Provided herein are antibodies against amyloid-beta (A?) oligomers, and methods of use thereof for the treatment of Alzheimer's disease (AD). In particular, neuronal expression of single-chain variable fragment (scFv) antibodies against A? oligomers is provided as a therapeutic approach in the treatment of AD.

Abstract: A biphenyl-containing diarylpyrimido compound of formula (I), which is prepared by reacting a 4-chloropyrimidine derivative with a biphenyl derivative in a solvent in the presence of a base. A pharmaceutically-acceptable salt of the compound (I) and a pharmaceutical composition containing the compound (I) or a pharmaceutically-acceptable salt thereof are also provided.

Abstract: A general-purpose approach to solving the core problems of detecting and predicting the actions of invisible actors, and the consequential challenges of intervention and prevention. The operational forecasting system is applied to data gathered from complex systems. The operational forecasting system uses novel early-warning signals that are based on anomalous behaviors of actors/agents that are observed, as they respond to those unobserved actors that are the source of systemic change. The operational forecasting system targets predicting when an event will occur, before it does, based on the anomalous behaviors of observed actors responding to those invisible actors that are creating the perturbation (i.e. the murmuration).

Abstract: This system and method of for providing a tunable orbital angular momentum system for providing higher order Bessel beams comprising: an acousto-optical deflector configured to receive an input beam, deflect a first portion of the input beam a first deflection angle relative to an axis of propagation and along an optical axis and deflect a second portion of the input beam a second deflection angle relative to the optical axis; a line generator disposed along the optical angle for receiving the first portion and the second portion of the input beam and provide an elliptical Gaussian mean; a log-polar optics assembly disposed along the optical angle for receiving the elliptical Gaussian beam and wrapping the elliptical Gaussian beam with an asymmetric ring; and, a Fourier lens configured to receive the wrapped elliptical Gaussian beam.