Abstract: The present invention involves a method of targeted imaging of a bacterial infection in a subject. In one embodiment, the method includes administering pyochelin (PcH) bound to a radiometal to the subject and imaging the infected area of the subject using a positron emission tomography-computed tomography (PET/CT) scan. In one embodiment, the pyochelin bound to a radiometal is selected from the group consisting of copper-64 bound pyochelin and gallium-68 bound pyochelin.

Abstract: Controlled release hydrogel formulations of one or more simvastatin metabolites 3?-hydroxy simvastatin (hSV), 6?-exomethylene simvastatin (eSV), 3?,5?-dihydrodiol simvastatin, 3?,5?-dihydrodiol simvastatin (dSV), simvastatin-beta-hydroxy acid (SVA), and methods for the treatment of patients suffering from injured or degenerating substantially avascular cartilaginous tissue.

Abstract: A novel genetically modified bacterium is disclosed. The bacterium has one or more anti-spike glycoprotein nanobodies on the outer membrane of the bacterium. In one embodiment, one or more of the anti-spike glycoprotein nanobodies have been fused with Intimin. In another embodiment, one or more of the anti-spike glycoprotein nanobodies have been fused with Lpp-OmpA.

Abstract: A method for performing an analysis related to potential rejection of an organ after transplantation in a subject is provided. The method involves identifying alloreactive/expanded CD8 T cell clones in either an allograft biopsy or a urine sample from the subject, wherein the T cell clones are identified using a single cell RNASeq (scRNAseq)/TCRseq test. In one embodiment, the analysis is selected from the group consisting of detection of rejection, diagnosis of rejection, treatment selection, therapeutic monitoring for rejection, identification of resistance to rejection treatment, and combinations thereof.

Abstract: Provided herein is a method of potentiating a cytotoxic effect of temozolomide against glioblastoma cells in a subject in need thereof, the method including administering to the subject a combination of: a therapeutically effective amount of temozolomide; and a non-cytotoxic amount of letrozole, wherein the letrozole potentiates the cytotoxic effect of the temozolomide against the glioblastoma cells. Also provided is a method of restoring sensitivity of temozolomide-resistant glioblastoma cells to temozolomide, including contacting the glioblastoma cells with a non-cytotoxic amount of letrozole and method of treating glioblastoma.

Abstract: A method of measuring sensor response for a sample is disclosed. The sample is exposed to an electrochemical aptamer-based (EAB) sensor, where the sensor includes an electrode and one or more aptamers having redox tags. Next, an interrogation is performed by applying an abrupt voltage pulse to the electrode. Two or more data samples are collected at different time values, where each data sample is a redox tag current value. Then at least one measure of the sample is identified using the data samples.

Abstract: A complex of curcumin with a polyphenolic macrocyclic host is disclosed. The macrocyclic host is a resorcin[4] arene. In one approach, the polyphenolic macrocyclic host is calix[8] arene. In another approach, the polyphenolic macrocyclic host is tert-butylcalix[8] arene.

Abstract: Methods of treating lung cancer in a subject in need thereof are provided, including administering to the subject an effective amount of an imidazolate gold compound according to Formula I, or a pharmaceutically acceptable salt, racemate, or enantiomer thereof: wherein R1 and R2 are independently selected from the group consisting of halo, cyano, hydroxyl, carboxyl, alkyl, aryl, esteryl, amidyl, and alkoxyl. Methods of inducing ferroptosis in a lung cancer cell and pharmaceutical compositions are also provided.

Abstract: A method of inverted SLA 3D printing with a printing device is disclosed. The method involves a) lifting a thin elastic membrane to an elevated position within a resin filled vat via vertical movement of an optical module, the resin filled vat, or both. b) allowing resin on the thin elastic membrane to cure as a current layer, the layer being attached to a printed part, and c) peeling the thin elastic membrane from the current layer by lowering the optical module, raising the resin filled vat, or both.

Abstract: A device and method for detecting the presence of, or measuring the concentration or amount of, at least one large analyte in a sample fluid. The device has at least one electrode, a sample fluid and a plurality of aptamers capable of binding to the analyte. The aptamers are physically bound to the device. In addition, the aptamers each include at least one redox tag and also, the aptamers have two or more binding portions that bind to two or more distinct binding sites on the analyte in the sample fluid.

Abstract: A method of diagnosing the likelihood of recurrence of clear cell renal cell carcinoma is provided. The method involves a) detecting the gene expression signatures of mitochondrial electron transport chain subunits, mitochondrial ribosomal proteins, major histocompatibility complex class II (MHC-II) proteins or combinations thereof in a kidney tumor tissue sample; and b) determining that the subject has an elevated risk of recurrence of clear cell renal cell carcinoma if the gene expression signatures include certain sequences. In another embodiment, the method uses copper levels to diagnose the likelihood of recurrence of clear cell renal cell carcinoma.

Abstract: A ventilation system for ventilation of a patient includes a patient interface device for attaching to the patient and a measuring and analysis device for measuring and analyzing breathing of the patient. The measuring and analysis device includes a connector housing defining a passage. A first portion of the connector housing is connected to the patient interface device. The measuring and analysis device further includes an air flow sensor and a pressure sensor disposed in the connector housing for measuring an air flow rate through the connector housing and a pressure in the connector housing respectively. The present device also includes a processor configured for data acquisition, data storage, data processing, and data output based on the air flow rate and the pressure, whereby the ventilation system is operable with real-time feedback based on the data output. A method for administering cardiopulmonary resuscitation is also provided.

Abstract: A device for detecting the presence of, or measuring the concentration or amount of, at least one analyte in a sample fluid is disclosed. The device includes at least one electrode; a sample fluid; and a plurality of affinity-based probes capable of binding to the analyte, wherein the affinity-based probes each carry a plurality of redox tags. Further, the detection or measurement of an analyte is caused by analyte binding to the affinity-based probe which further causes a change in electron transfer from the redox tags.

Abstract: A method for preparing an autologous pancreatic tumor organoid and immune cell co-culture is provided, including: culturing pancreatic tumor cells obtained from a patient in a culture medium to provide a pancreatic tumor organoid and an organoid-conditioned medium; pulsing dendritic cells derived from the patient with a portion of the organoid-conditioned medium; contacting the pulsed dendritic cells with cytotoxic T lymphocytes (CTLs) obtained from the patient in the organoid-conditioned medium to activate the CTLs; isolating the activated CTLs; and co-culturing the pancreatic tumor organoid with the activated CTLs and myeloid derived suppressor cells (MDSCs) derived from the patient, to obtain an autologous pancreatic tumor organoid and immune cell co-culture that mimics the patient's pancreatic tumor microenvironment. Also provided are co-cultures obtained by the disclosed methods and methods of screening and determining whether a patient is likely to benefit from a candidate therapy.

Abstract: Certain aspects of the disclosure provide systems and methods for diagnosing and treating chronic liver disease and portal vein thrombosis. In particular, methods may include measuring levels of one or more proteins, including factor V, factor VIII, protein C, protein S, D-dimer, sP-selectin, or asTF in a biological sample from a subject and determining a CLD score based on the levels of the one or more proteins, whereby, a CLD stage may be determined based on the CLD score. In some aspects, methods may include determining a PVT score based on levels of the one or more proteins in the biological sample. In some aspects, methods further include treating CLD and/or PVT.

Abstract: A device for modeling a brain tumor microenvironment and testing therapeutic efficacy is provided. The device includes a vascular tissue model and an ultrasound device that is capable of delivering focused ultrasound insonation. The vascular tissue model includes a rigid 3D printed scaffold. The scaffold includes one or more scaffold microfluidic channels, two or more inlets, and a central chamber. The central chamber contains a hydrogel or other biocompatible scaffolds. The hydrogel includes one or more hydrogel microfluidic channels as well as living cells.

Abstract: A novel endonasal method for occipitocervical fusion is provided. This new method provides a minimally invasive advance in the ability of surgeons to treat pathology of the craniocervical junction. The method uses an implant system comprising a body having a spacer section that engages with the occipital condyle and a plate section that engages with the C1 lateral mass. Fasteners fix the plate section with the occipital condyle the plate section with the C1 lateral mass.

Abstract: A method for stimulating and sensing sweat includes dosing a sweat stimulant to skin effective to generate sweat at a generation rate of at least 0.1 nL/min/gland for a duration of six hours or more per dose of the sweat stimulant, and sensing generated sweat using a device placed on the skin comprising at least one sensor specific to an analyte in sweat. The dosing comprises at least one of: applying a charge density of less than 320 mC/cm2/day or applying a one-time dose of the sweat stimulant.

Abstract: A method for obtaining a magnetic resonance imaging (MRI) image of an object is provided. The method includes applying a MRI sequence to a target area in the object, receiving magnetic resonance (MR) signals from the target area, acquiring, in one k-space strategy, a first set of k-space lines based on the MR signals while nuclear spins in the target area are in a transient state, acquiring, in another k-space strategy, a second set of k-space lines based on the MR signals while the nuclear spins in the target area are in a steady-state or a mixed state of the transient state and the steady-state, and reconstructing the MRI image based on the first set of k-space lines and the second set of k-space lines.

Abstract: A method for detecting blood-tissue barrier breakdown or disruption is provided. The method includes acquiring first image data of a target region of a subject with a predetermined spatial resolution; administrating exogenous or endogenous tracer into the subject; acquiring second image data of the target region with the predetermined spatial resolution after administration of the tracer; differentiating healthy tissue and pathological tissue with blood-tissue barrier breakdown in the target region based on the first image data and the second image data; and visualizing or analyzing the blood-tissue barrier breakdown of pathological tissue based on the differentiation.