Abstract: A method of inverted SLA 3D printing with a printing device is disclosed. The method involves a) lifting a thin elastic membrane to an elevated position within a resin filled vat via vertical movement of an optical module, the resin filled vat, or both. b) allowing resin on the thin elastic membrane to cure as a current layer, the layer being attached to a printed part, and c) peeling the thin elastic membrane from the current layer by lowering the optical module, raising the resin filled vat, or both.

Abstract: A device and method for detecting the presence of, or measuring the concentration or amount of, at least one large analyte in a sample fluid. The device has at least one electrode, a sample fluid and a plurality of aptamers capable of binding to the analyte. The aptamers are physically bound to the device. In addition, the aptamers each include at least one redox tag and also, the aptamers have two or more binding portions that bind to two or more distinct binding sites on the analyte in the sample fluid.

Abstract: A method of diagnosing the likelihood of recurrence of clear cell renal cell carcinoma is provided. The method involves a) detecting the gene expression signatures of mitochondrial electron transport chain subunits, mitochondrial ribosomal proteins, major histocompatibility complex class II (MHC-II) proteins or combinations thereof in a kidney tumor tissue sample; and b) determining that the subject has an elevated risk of recurrence of clear cell renal cell carcinoma if the gene expression signatures include certain sequences. In another embodiment, the method uses copper levels to diagnose the likelihood of recurrence of clear cell renal cell carcinoma.

Abstract: A ventilation system for ventilation of a patient includes a patient interface device for attaching to the patient and a measuring and analysis device for measuring and analyzing breathing of the patient. The measuring and analysis device includes a connector housing defining a passage. A first portion of the connector housing is connected to the patient interface device. The measuring and analysis device further includes an air flow sensor and a pressure sensor disposed in the connector housing for measuring an air flow rate through the connector housing and a pressure in the connector housing respectively. The present device also includes a processor configured for data acquisition, data storage, data processing, and data output based on the air flow rate and the pressure, whereby the ventilation system is operable with real-time feedback based on the data output. A method for administering cardiopulmonary resuscitation is also provided.

Abstract: A device for detecting the presence of, or measuring the concentration or amount of, at least one analyte in a sample fluid is disclosed. The device includes at least one electrode; a sample fluid; and a plurality of affinity-based probes capable of binding to the analyte, wherein the affinity-based probes each carry a plurality of redox tags. Further, the detection or measurement of an analyte is caused by analyte binding to the affinity-based probe which further causes a change in electron transfer from the redox tags.

Abstract: A method for preparing an autologous pancreatic tumor organoid and immune cell co-culture is provided, including: culturing pancreatic tumor cells obtained from a patient in a culture medium to provide a pancreatic tumor organoid and an organoid-conditioned medium; pulsing dendritic cells derived from the patient with a portion of the organoid-conditioned medium; contacting the pulsed dendritic cells with cytotoxic T lymphocytes (CTLs) obtained from the patient in the organoid-conditioned medium to activate the CTLs; isolating the activated CTLs; and co-culturing the pancreatic tumor organoid with the activated CTLs and myeloid derived suppressor cells (MDSCs) derived from the patient, to obtain an autologous pancreatic tumor organoid and immune cell co-culture that mimics the patient's pancreatic tumor microenvironment. Also provided are co-cultures obtained by the disclosed methods and methods of screening and determining whether a patient is likely to benefit from a candidate therapy.

Abstract: A device for modeling a brain tumor microenvironment and testing therapeutic efficacy is provided. The device includes a vascular tissue model and an ultrasound device that is capable of delivering focused ultrasound insonation. The vascular tissue model includes a rigid 3D printed scaffold. The scaffold includes one or more scaffold microfluidic channels, two or more inlets, and a central chamber. The central chamber contains a hydrogel or other biocompatible scaffolds. The hydrogel includes one or more hydrogel microfluidic channels as well as living cells.

Abstract: Certain aspects of the disclosure provide systems and methods for diagnosing and treating chronic liver disease and portal vein thrombosis. In particular, methods may include measuring levels of one or more proteins, including factor V, factor VIII, protein C, protein S, D-dimer, sP-selectin, or asTF in a biological sample from a subject and determining a CLD score based on the levels of the one or more proteins, whereby, a CLD stage may be determined based on the CLD score. In some aspects, methods may include determining a PVT score based on levels of the one or more proteins in the biological sample. In some aspects, methods further include treating CLD and/or PVT.

Abstract: A novel endonasal method for occipitocervical fusion is provided. This new method provides a minimally invasive advance in the ability of surgeons to treat pathology of the craniocervical junction. The method uses an implant system comprising a body having a spacer section that engages with the occipital condyle and a plate section that engages with the C1 lateral mass. Fasteners fix the plate section with the occipital condyle the plate section with the C1 lateral mass.

Abstract: A method for obtaining a magnetic resonance imaging (MRI) image of an object is provided. The method includes applying a MRI sequence to a target area in the object, receiving magnetic resonance (MR) signals from the target area, acquiring, in one k-space strategy, a first set of k-space lines based on the MR signals while nuclear spins in the target area are in a transient state, acquiring, in another k-space strategy, a second set of k-space lines based on the MR signals while the nuclear spins in the target area are in a steady-state or a mixed state of the transient state and the steady-state, and reconstructing the MRI image based on the first set of k-space lines and the second set of k-space lines.

Abstract: A method for stimulating and sensing sweat includes dosing a sweat stimulant to skin effective to generate sweat at a generation rate of at least 0.1 nL/min/gland for a duration of six hours or more per dose of the sweat stimulant, and sensing generated sweat using a device placed on the skin comprising at least one sensor specific to an analyte in sweat. The dosing comprises at least one of: applying a charge density of less than 320 mC/cm2/day or applying a one-time dose of the sweat stimulant.

Abstract: A method for detecting blood-tissue barrier breakdown or disruption is provided. The method includes acquiring first image data of a target region of a subject with a predetermined spatial resolution; administrating exogenous or endogenous tracer into the subject; acquiring second image data of the target region with the predetermined spatial resolution after administration of the tracer; differentiating healthy tissue and pathological tissue with blood-tissue barrier breakdown in the target region based on the first image data and the second image data; and visualizing or analyzing the blood-tissue barrier breakdown of pathological tissue based on the differentiation.

Abstract: A door control apparatus is provided. The door control apparatus comprises a processor, a frame that includes a top frame portion and a bottom frame portion, a door control arm that is integrated as part of the frame and configured to move vertically between the top frame portion and the bottom frame portion, the door control arm including a door handle contact protrusion having a curve shaped end part, the door handle contact protrusion extending substantially perpendicularly relative to the frame, and a door interaction arm that is disposed on a surface of the frame and extends substantially perpendicularly relative to the frame.

Abstract: A sensing device for detecting a characteristic of an analyte in a sample solution and method of making the same is provided. The device includes one or more sensors configured to measure a characteristic of an analyte in a sample. The device further includes a sample solution that contains the analyte and one or more interfering solutes, wherein the interfering solute reduces a performance characteristic of the one or more sensors. The device further includes a sensor solution in fluidic communication with the one or more sensors. The device further includes a hydrophobic barrier that separates the sample solution from the one or more sensors, wherein the hydrophobic barrier is permeable to the analyte and impermeable to the interfering solute, and wherein the hydrophobic barrier is in fluidic communication with the sample solution and the sensor solution.

Abstract: Provided herein is a device for rapid fixation and embedding of a biological specimen, the device including a barrel having a first end and a second end, the first end receiving a plunger and the second end opening to a compartment defining a block mold; and a plunger for inserting in the barrel, the plunger having a first end, a second end, and an interior conduit connecting the first and second ends, wherein the first end of the plunger connects to a vacuum source and the second end of the plunger includes a filter barrier between the interior conduit of the plunger and a receptacle for receiving, fixing, and embedding the specimen, the receptacle defined inside the barrel when the plunger is disposed in the barrel. Also provided are a system and method for rapid fixation and embedding of a biological specimen.

Abstract: A novel method for treating hyponatremia or polycystic kidney disease in a mammal is disclosed. The method involves administering to the mammal in need of such treatment a therapeutically effective amount of an adenine analog selected from the group consisting of: 2-chloro-6-aminopurine, its tautomer, racemate, optical isomer and/or pharmaceutically or nutritionally acceptable salt thereof, and a pharmaceutical carrier, 6-(dimethylamino) purine, its tautomer, racemate, optical isomer and/or pharmaceutically or nutritionally acceptable salt thereof, and a pharmaceutical carrier; and combinations thereof.

Abstract: A novel ROS-responsive prodrug is provided. The prodrug utilizes a unique modified oxalate linker conjugated to 4-aminophenol, which can enhance the reaction kinetics for intracellular ROS within tumor tissues while keeping the modified oxalate backbone stable with amide bond under very low ROS level.

Abstract: A method of making a polyurethane polymerized high internal phase emulsion (polyHIPE) is disclosed. The method involves preparing a polyurethane prepolymer and crosslinking the polyurethane prepolymer using a thiol-alkene Michael addition, producing a polyurethane polymerized high internal phase emulsion. In one embodiment, the polyurethane prepolymer is prepared by reacting diisocyanate and trimethylolpropaneallylether (TMPAE).

Abstract: Novel lentivirus packaging systems engineered with a synthetic gene network having a positive feedback loop to amplify the expression of virus genes are provided. When co-transfected into a host cell with a transfer plasmid and envelope vector, extremely high viral titers are achieved when compared to transfection of a host cell with conventional third generation packaging systems. Methods for enhancing production of lentivirus, compositions comprising high titer lentivirus, and therapeutic methods based on delivery of lentiviral nucleic acid to target cells are also provided.

Abstract: A method for spatial resolution enhanced imaging is provided. The method includes determining a target region of a subject being imaged; estimating an achievable spatial resolution; selecting an increased spatial resolution that is greater than a predetermined spatial resolution and equal to or less than the achievable spatial resolution; and acquiring image data of the target region with the increased spatial resolution. A method for enhancing the contrast between the region of interest and other tissue region in the image data acquired with the increased spatial resolution and other tissue region in image data acquired with the predetermined spatial resolution due to the intrinsic partial volume effect is also provided.