Abstract: Various examples are provided related to software and hardware architectures that enable lightweight and real-time Denial-of-Service (DoS) and Distributed Denial-of-Service (DDoS) attack detection. In one example, among others, a method for detection and localization of denial-of-service (DoS) attacks includes detecting, by a router of an intellectual property (IP) core in a network-on-chip (NoC) based system-on-chip (SoC) architecture, a compromised packet stream based at least in part upon a packet arrival curve (PAC) associated with the router; identifying, by the IP core, a candidate IP core in the NoC as a potential attacker based at least in part upon a destination packet latency curve (DLC) associated with the IP core; and transmitting, by the router, a notification message indicating that the candidate IP core is the potential attacker to a router of the candidate IP core.

Abstract: Modulators of Nuclear Receptor Subfamily 4 Group A Member 1 (NR4A1) are described as well as their use as therapeutic agents for disease, disorders, or symptoms thereof, including those where modulation of NR4A1 is implicated. Such disease and disorders include cancer.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed in (e.g., continuously printed using a moving dispensing nozzle) a yield-stress support bath to form an intermediate article. The intermediate article may be liquid or only partially coagulated after being printed into the yield-stress support bath. The yield-stress support bath may be at least partially disposed within a container, and the container may be immersed in a post-treatment coagulation solution to remove some or all of the solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: Described herein are compositions and methods for making and using recombinant bacteria that are capable of regulated attenuation and/or regulated expression of one or more antigens of interest.

Abstract: The invention provides American chestnut leaf compositions comprising oxalate oxidase. The compositions reduce oxalate levels in dietary sources of oxalate and are useful in reducing oxalate levels and reducing stone disease in patients. The invention provides methods of extracting and purifying oxalate oxidase from American chestnut leaf. The invention provides methods of extracting oxalate from biological samples.

Abstract: Aspects of the disclosure relate to methods and compositions for treating pancreatic cancer (e.g., islet cell tumors). In some aspects, adeno-associated virus (AAV) may be used to deliver an interfering RNA that targets thymidylate synthase (TS).

Abstract: Provided herein are methods related to co-packaging of multiple rAAV particles, e.g., by introducing multiple nucleic acid vectors encoding proteins or polypeptides or RNAs of interest into a single cell preparation.

Abstract: Systems and methods are configured to generate a frequency map representing a density of objects found in regions of a sample that may be used in setting parameters for imaging the regions. Various embodiments involve binarizing the pixels for a raw image of the sample to transform the image into binary data. Run-length encoded components are identified from the data for dimensions of the raw image. Each component is a length of a sequence of adjacent pixels found in a dimension with the same value in the binary data. A projection of the image is then generated from projection values for the dimensions. Each projection value provides a measure of the density of objects present in a dimension with respect to the components identified for the dimension. This projection is used to identify a level of density for each region of the sample from which the frequency map is generated.

Abstract: Aspects of the disclosure relate to methods and compositions (e.g., biomarkers) useful for diagnosing pre- symptomatic amyotrophic lateral sclerosis (ALS) and/or frontotemporal dementia (FTD), and for monitoring the progression of ALS/FTD in subjects diagnosed with the disease. Methods of treating neurodegenerative diseases (e.g., ALS/FTD) are also described.

Abstract: The protein targeted medicine, Dovitinib, has been repurposed to target a non-coding RNA by using selection and computational drug design via Inforna. Selectivity was achieved for pre-miR-21 by endowing the medicine with the ability to recruit RNA quality control enzymes to cleave the target.

Abstract: Described herein are compositions and methods for making and using recombinant bacteria that are capable of regulated attenuation, regulated expression of one or more antigens of interest, and/or N-glycan modification of secreted/surface antigens.

Abstract: Adeno associated viral (AAV) particles are emerging as a useful vehicle for gene delivery to various organs and tissues, one of them being the retina. Provided here are variant AAV (for example, variant serotype 2 (AAV2)) capsid proteins and variant capsid protein containing particles with enhanced ability to transduce retinal cells.

Abstract: Aspects of the disclosure relate to chimeric antigen receptors (CARs) comprising an antigen binding domain (e.g., anti-TSHR), transmembrane domain (e.g., CD28), and a cytoplasmic domain (e.g., CD27, CD-137, etc.) and a safety mechanism comprising an inducible apoptosis trigger. In some aspects, the disclosure relates to use of the CARs in T cells, compositions, kits and methods for the treatment of thyroid cancers.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures, without use of a support bath, and the like. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed (e.g., continuously printed using a moving dispensing nozzle) in air without the use of supports (e.g., without the use of a support bath, a concurrently printed support posts, or the like) while a nebulized coagulation agent is dispersed alongside the printed polymer solution to at least partially coagulate the polymer solution and form an intermediate article. The self-supporting intermediate article may then be immersed in a post-printing coagulation solution to remove some or all of the remaining solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: The disclosure relates to modification of a heat resistant cytoplasmic heat stable 6-phosphogluconate dehydrogenase (6PGDH) enzyme by fusing the cytoplasmic 6PGDH enzyme in frame to a plastid-targeting sequence. This modification allows the import of the cytoplasmic 6PGDH enzyme into plastids of a plant cell. Polynucleotides encoding and expressing the modified cytoplasmic 6PGDH enzymes are provided. The disclosure further provides transgenic plants and seeds containing the disclosed polynucleotides and expressing the modified cytoplasmic 6PGDH enzymes during development. The invention further relates to methods for developing a transgenic plant that has increased heat resistance and yield during heat stress.

Abstract: The disclosure provides methods for the diagnosis of Huntington's disease. In some embodiments, the method comprises detecting one or more repeat associated non-ATG (RAN) proteins in a biological sample.

Abstract: A high-frequency dynamic pressure transducer calibration system and method is provided. The method directs a source onto a diaphragm of a dynamic pressure transducer. An oscillating voltage at a target frequency (or range of frequencies) is generated. The oscillating voltage is coupled to an electrical connector of the dynamic pressure transducer. A deflection pattern of the diaphragm is recorded. The dynamic pressure transducer is calibrated by correlating magnitude of the deflection pattern with the oscillating voltage as a function of the target frequency (or range of frequencies).

Abstract: The subject invention provides therapeutic compositions, and uses thereof for the treatment or amelioration of injury to small intestine mucosa. In preferred embodiments, the composition comprises one or more nutrients and/or electrolytes that acquire or retain considerable absorptive capacity.

Abstract: Various examples are provided related to penetrating radar based upon precursors. In one example, a method includes transmitting a radio frequency (RF) signal; and receiving a return signal associated with the RF signal, where the return signal is a precursor having no exponential decay. The precursor can be one of a sequence of precursors, which can be used to improve resolution of the system. The RF signal can be a short pulse generated by an RF front end, without automatic level control. The return signal can be processed without filtering.

Abstract: Described herein are apparatuses, systems, and methods for fabricating tissue constructs, such as by fabricating perfusable tissue constructs by embedding a sacrificial material into a composite matrix yield stress support bath. A composite matrix bath can include a microgel filler and a hydrogel precursor. An extrusion tip can be used for embedded printing of perfusable tissue constructs by disposing sacrificial material into the composite matrix bath while the extrusion tip travels along a predefined course through the composite matrix bath. This sacrificial material can be the printed tissue construct or can be removed to render the matrix bath a perfusable tissue construct. The composite matrix bath can include acellular or cell-laden hydrogels. The sacrificial material can include a salt and a physiological buffer or a non-cytotoxic porogen material. The hydrogel precursor can include at least one of gellan and gelatin. Cross-linking can be carried out chemically, thermally, enzymatically, or physically.