Abstract: The present invention relates to Ras suppressors, in particular the Ras suppressor SUR-8. The present invention provides isolated nucleotide sequence encoding SUR-8, isolated SUR-8 peptides, antibodies that specifically bind SUR-8, methods for the detection of SUR-8, methods for producing SUR-8 transgenic animals, non-human animals expressing SUR-8, and methods for screening compounds for the ability to alter SUR-8 associated signal transduction.

Abstract: A method for in-room computer reconstruction of a three-dimensional (3-D) coronary vascular tree from routine biplane angiograms acquired at arbitrary angles and without using calibration objects.

Abstract: Plasminogen activator acts as an anti-inflammatory agent by inhibiting generation of superoxide anion by a mechanism that is not related to L-arginine, is not dependent on thrombolytic activity, and is not a function of oxygen free radical scavenging. Moreover, in in vivo models of inflammation, treatment with plasminogen activator reduces edema without inhibiting neutrophil infiltration in in vivo models of inflammation.

Abstract: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.

Abstract: A novel method of treating and preventing diseases is provided. In particular, compositions and methods of blocking diseases associated with aberrant levels of nitric oxide and facilitated by a serine proteolytic (SP) activity are disclosed, which consist of administering to a subject a therapeutically effective amount of a compound having a serine protease inhibitory activity. Among effective compounds are &agr;1-antitrypsin and synthetic drugs mimicking some or all of the actions of &agr;1-antitrypsin.

Abstract: Pharmaceutical compositions for the delivery of pharmacologically active proteins are provided by the present invention. The compositions of the present invention comprise a polymeric matrix having thermal gelation properties in which is incorporated a discrete suspension of at least one biologically active macromolecular polypeptide which retains greater than 90 percent of its biological activity. Furthermore, the concentration of the macromolecular polypeptide is greater than 0.5 percent by weight of the composition.

Abstract: An apparatus and method for controlling the pore structure of thin polymeric sheets, such as porous membranes and thin films, during solvent casting or interfacial polymerization. The invention may be used to reduce or eliminate macrovoid pore defects from solvent-cast or interfacially polymerized polymeric membranes used in a variety of separations or controlled release tasks or to create a desired pore structure in porous thin films, such as may be used in breathable garments, surgical dressings, and screen printing. A nonuniform electric field is generated at one or more locations in proximity to a liquid film in which a desired pore structure is to be formed. Due to the difference in dielectric constant between the liquid within the pores and that in the surrounding liquid, the electric field causes an attractive or repulsive force on the evolving pores.

Abstract: A color management method/apparatus generates image color matching and International Color Consortium (ICC) color printer profiles using a reduced number of color patch measurements. Color printer characterization, and the generation of ICC profiles usually require a large number of measured data points or color patches and complex interpolation techniques. This invention provides an optimization method/apparatus for performing LAB to CMYK color space conversion, gamut mapping, and gray component replacement. A gamut trained network architecture performs LAB to CMYK color space conversion to generate a color profile lookup table for a color printer, or alternatively, to directly control the color printer in accordance with the a plurality of color patches that accurately. represent the gamut of the color printer.

Abstract: The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis, and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.

Abstract: Provided is a sustained release composition for sustained release of a pharmaceutical substance. The composition includes a biocompatible polymer that is highly amorphous and a pharmaceutical substance in a hydrophobic ion complex with an amphiphilic material. Also provided a compressed antisolvent method for manufacturing the composition, various product forms incorporating the composition and various uses for the composition.

Abstract: The present invention provides TRT antisense oligonucleotides, methods of detecting TRT, methods of diagnosing telomerase-related conditions, methods of diagnosing and providing a prognosis for cancer, and methods of treating telomerase-related conditions, including cancer.

Abstract: Methods are disclosed for treating heart failure. Methods are disclosed for screening pharmacologicals that inhibit the down-regulatory activity of AUF1 or AUF1-related polypeptide for &bgr;-adrenergic activity. Methods are disclosed for treating a patient with a high level of the AUF1 or AUF1-related gene.

Abstract: Apparatus for increasing color resolution and quality in digital imaging systems temporally modulates red, green and blue light, detects all three colors at each pixel, and band pass filters the detected light to extract values for red, green and blue at each pixel. A detector which preserves the modulation, such as a complementary metal oxide semiconductor detector is used. Alternatively, if an integrating detector array such as a charge coupled device (CCD) is used, the three illumination sources are switched on and off sequentially, and the detector array switches to different integrating circuits for each color. Measurement of the range provides full color, three-dimensional, image data.

Abstract: Methods of forming polymer particles in situ from polymer precursors such as monomers or oligomers, comprising exposing a composition comprising at least one polymer precursor, a solvent or solvent mixture, and an antisolvent or antisolvent mixture to photoradiation under conditions whereby particles are formed are provided. The polymer precursor may be photosensitive, or a separate polymerization initiator may be used. In a preferred embodiment, the polymer precursor is insoluble in the antisolvent or antisolvent mixture and the solvent or solvent mixture is soluble in the antisolvent or antisolvent mixture at the concentrations used. Polymer particles comprising a polymer and a bioactive material are also provided. The polymer may be erodable, and the polymer particles formed may be used in a variety of applications, including controlled release of bioactive materials such as drugs.

Abstract: An apparatus and method for on-column analysis in flash chromatography. The detection scheme utilizes refractive index changes as analytes move through an illuminated region of a chromatography column. The column packing material is a diffuse scattering medium when the refractive index of the solvent is significantly different than that of the packing material. The magnitude of the signal depends on the degree to which the refractive index mismatch is changed as an analyte passes through the illuminated region. This detection scheme provides a simple, inexpensive means for monitoring the end of a flash chromatography column to determine the exit time of the species of interest, thus greatly reducing the post-column analysis time. Additionally, the detector is movable along the length of the column, offering the potential to monitor separations as they occur.

Abstract: An icing hazard avoidance system and method utilizes a forward-looking, single frequency, dual-polarization pulse radar system as well as temperature sensing means to determine if supercooled liquid water (SLW) drops are present in an aircraft's flight path. A processor determines the ratio of co-polarized and cross-polarized returns. This ratio and the temperature of the atmosphere indicate if SLW drops exists. If an icing hazard is determined, an indicator in the cockpit of the aircraft alerts pilots so that avoidance measures may be taken.

Abstract: The present invention relates to methods for treating neurological-urological conditions. This is accomplished by administration of at least one neurotoxin.

Abstract: Provided is a method for preparing a true, homogeneous solution of a pharmaceutical substance dissolved in an organic solvent in which the pharmaceutical substance is not normally soluble. Solubilization is obtained by forming a hydrophobic ion pair complex involving the pharmaceutical substance and an amphiphilic material. The resulting organic solution may be further processed to prepare pharmaceutical powders. A biodegradable polymer may be co-dissolved with the pharmaceutical substance and the amphiphilic material and may be incorporated into a pharmaceutical powder. A preferred method for preparing pharmaceutical powders is to subject the organic solution to gas antisolvent precipitation using a supercritical gas antisolvent such as carbon dioxide. Also provided is a method for making hollow particles having a fiber-like shape which would provide enhanced retention time in the stomach if ingested by a human or animal host.

Abstract: The present invention is a methodology for analyzing and evaluating a sample text, such as essay(s), or document(s). This methodology compares sample text to a reference essay(s), document(s), or text segment(s) within a reference essay or document. The methodology analyzes the amount of subject-matter information in the sample text, analyzes the relevance of subject matter information in the sample and evaluates the semantic coherence of the sample. This methodology presumes there is an underlying, latent semantic structure in the usage of words. The method parses and stores text objects and text segments from the sample text and reference text into a two-dimensional data matrix. A weight is computed for each text object and applied to each data matrix cell value. The method performs a singular value decomposition on the data matrix, which produces three trained matrices. The method computes a vector representation of the sample text and reference text using the three trained matrices.

Abstract: The present invention discloses a transgenic mouse that is a model for congestive heart failure. The disclosed mouse is a subset of a transgenic mouse line that is a model for hypertrophic cardiomyopathy. Also disclosed are a recombinant nucleic acid molecule encoding a transgene, and methods of using the transgenic mouse model to study congestive heart failure and conditions and treatments related thereto.