Abstract: The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome of E coli prompted by strong promoter Ptrc to reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Ptrc on the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.

Abstract: Methods of treating subjects having disorders associated with mutations in the FXN gene and/or having reduced expression of frataxin protein, e.g., Friedreich ataxia (FA). Generally, the methods include administering a therapeutically effective amount of an agent that increases expression of frataxin protein as described herein, e.g., an inhibitor of a FXN Repressing Factor (FXN-RF).

Abstract: An energy storage device is provided which includes a supercapacitor first electrode, a supercapacitor second electrode, a first electrolyte, a metal electrode, and a hydrophobic layer. The supercapacitor first electrode, the supercapacitor second electrode, and the first electrolyte together form a supercapacitor. The metal electrode is spaced apart from the supercapacitor first electrode to form a first gap, the metal electrode and the supercapacitor second electrode form an Ohmic contact. The hydrophobic layer is located on at least one portion of a surface of the supercapacitor first electrode and/or at least one portion of a surface of the supercapacitor second electrode.

Abstract: The present disclosure relates to methods for the chronic treatment of dyssynchronous cardiac dysfunction (including non-arrhythmia cardiac dysfunction such as heart failure, cardiomyopathy, viral myocarditis, ischemia induced cardiomyopathy, ischemia, chemotherapy, pacing induced cardiomyopathy or ion channel mutation related dysfunction) and for the prevention or treatment of diseases or conditions associated with dyssynchronous cardiac dysfunction. The method comprises chronically administering to a patient an effective amount of dantrolene, or derivative or analogs thereof (such as azumolene).

Abstract: Disclosed are compositions and methods for targeted treatment of cancers, such as TLR9-expressing cancers. In particular, molecules containing a TLR9 targeting ligand, such as a CpG oligodeoxynucleotide, that target lytic peptides to TLR9-expressing malignant cells are disclosed.

Abstract: A method and system for delivering a molecule to a specific area of a tissue by controlling temperature and impedance is presented. The method is generally comprised of applying heat to a biological structure, such as cells or tissues, to heat the biological structure to a preset temperature after which at least one electroporation pulse is administered to the biological structure. Impedance is measured as a feedback control mechanism after each pulse and pulse parameters are adjusted accordingly until desired impedance is reached. The system generally comprises an electroporation system capable of generating at least one pulse, measuring impedance and measuring temperature. The method may be used to deliver a molecule such as a vaccine or therapeutic to a biological structure, such as for prevention or treatment of SARS-CoV-2 infection.

Abstract: A method of depositing a coating layer comprising gallium nitride on a substrate comprising the steps of: (a) providing the substrate having a plurality of side walls and valleys; (b) forming a first layer of gallium nitride deposited on the substrate, by reacting gaseous trimethylgallium and ammonia at a temperature ranging from 400 to 500° C., such that the first layer is formed on the side walls and the valleys; and (c) forming a second layer of gallium nitride deposited on top of the first layer, by reacting gaseous trimethylgallium and ammonia at a temperature ranging from 1000 to 1200° C., to obtain the coating layer comprising the first layer of gallium nitride and the second layer of gallium nitride at a thickness ranging from 3.0 to 4.5 ?m.

Abstract: Methods and compositions are provided for combined transplantation of a solid organ and hematopoietic cells to a recipient, where tolerance to the graft is established through development of a persistent mixed chimerism. An individual with persistent mixed chimerism, usually for a period of at least six months, is able to withdraw from the use of immunosuppressive drugs after a period of time sufficient to establish tolerance.

Abstract: Described herein are flash nanoprecipitation methods capable of encapsulating hydrophobic molecules, hydrophilic molecules, bioactive protein therapeutics, or other target molecules in amphiphilic copolymer nanocarriers.

Abstract: A palladium selective chemosensor based on a fluorescein-allyloxy benzene scaffold and a method of detecting palladium ions in a fluid sample with the chemosensor, whereby the fluid sample is contacted with a solution that includes water and the chemosensor to form a mixture. An ultraviolet visible absorption profile and/or a fluorescence emission profile of the mixture is measured to determine a presence or absence of palladium ions in the fluid sample, wherein the chemosensor has an ultraviolet visible absorption peak at 315 to 325 nm and a fluorescence emissions peak at 380 to 400 nm in the solution, and wherein a bathochromic shift in the ultraviolet visible absorption peak to 338 to 342 nm in the mixture and/or a bathochromic shift in the fluorescence emissions peak to 530 to 550 nm in the mixture indicates the presence of palladium ions in the fluid sample.

Abstract: Disclosed are a synchronous movement apparatus of tracks in a wellbore inspection system and a control method thereof. The synchronous movement apparatus includes an upper moving track, a lower moving track, an upper wire rope moving device, a lower wire rope moving device, and a control device; the upper moving track and the lower moving track are correspondingly embedded into an inner wall of a wellbore, and the upper moving track is located above the lower moving track; the upper wire rope moving device is fitted in the upper moving track, and the lower wire rope moving device is fitted in the lower moving track; the upper moving track and the lower moving track have the same structure and each include a track body. A rolling face is arranged on the track body, and grooves are evenly distributed on the rolling face along the extending direction of the track body.

Abstract: An embodiment in accordance with the present invention includes an EGS configured to allow the commercial production of electrical energy. One criteria of an EGS according to the present invention is that the temperature and volume of the fluids extracted are sufficiently high and large enough as to allow the commercial production of electrical energy. The system is able to operate for at least N years before the extracted fluid falls below the minimum temperature needed for energy production. Additionally, fractures are separated from each other by a sufficiently large volume of rock (Vcrit) relative to the fractures surface area such that the ratio of the rate of heat extraction to the rate of heat supply controlled by the thermal conductivity of the rock is such that the intervening rock is cooled at a rate that is sufficiently slow to be economic.

Abstract: A device for achieving a core part press-down technology in a continuous casting round billet solidification process includes a plurality of round billet radial press-down devices distributed along an axial array of round billets outside a press-down interval of the round billets. The press-down interval is an area from 0.65 of a solid phase ratio of the round billets to solidification end points. Each round billet radial press-down device includes a plurality of press-down rollers. A forming hole for extruding the round billets is formed between the press-down rollers. Two adjacent round billet radial press-down devices are arranged in the manner of staggering. The device can effectively solve the defect problems of porosity, segregation and the like in the core of the continuous casting round billets, the yield of the continuous casting round billets is increased, and the production cost is reduced.

Abstract: A method of treating opioid use disorder involves administering a selective butyrylcholinesterase (BChE) inhibitor.

Abstract: Methods and kits for the detection of toxic cyanobacteria in a sample by analyzing the sample for the presence antibodies raised in a host, where the presence of antibodies is indicative of toxic cyanobacteria, are described.

Abstract: The present invention relates to compositions and methods for treating neurodegenerative conditions using GLP-1r agonists. In certain embodiments, long-acting GLP-1r agonists have neuroprotective and disease modifying effects on the central nervous system.

Abstract: This invention relates to modified parvovirus capsid proteins with enhanced transduction efficiency, viral vectors comprising the same, and methods of using the same for delivery of nucleic acids to a cell or a subject.

Abstract: This application relates to translating mechanical energy from a low-speed rotor to a high-speed rotor or vice versa in a magnetic gearbox. More specifically, this application discloses various embodiments of a coaxial magnetic gearbox with flux concentration Halbach rotors. In certain implementations, the device further comprises a circular back iron (or other ferromagnetic material) disposed concentrically along the axis of the cylindrical magnetic gearing device. In such embodiments, this flux concentration back iron (or ferromagnetic pole) improves the torque density and can also help retain the magnets in place.

Abstract: Coaxial electrospinning is used to encapsulate a chitin-lignin based hybrid gel with polycaprolactone (PCL). Antibiotics and/or other bioactive agents loaded into the core and/or shell layer of the fibrous platform are released in a controlled and sustained manner that effectively inhibits both Gram-positive and Gram-negative bacteria without cytotoxicity to mammalian cells. The PCL shell layer provides longer life for the CL gels in a wet environment and allows sustainable drug release. The PCL-coated CL nanofiber scaffolds can be loaded with antimicrobial nanoparticles, antibiotics, oxygen-releasing agents, antioxidants and/or growth factors that promote healing when used as a controlled drug release dressing for chronic wounds, such as diabetic ulcers.

Abstract: The present disclosure relates to a pre-5th-Generation (5G) or 5G communication system to be provided for supporting higher data rates Beyond 4th-Generation (4G) communication system such as Long Term Evolution (LTE). A method of decoding a signal in a communication system includes receiving an encoded bit-stream corresponding to message bits and first Cyclic Redundancy Check (CRC) bits, obtaining a codeword through a traceback for at least part of the encoded bit-stream, generating second CRC bits by performing CRC encoding on the codeword, and performing decoding based on at least part of the second CRC bits.