Abstract: The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one protein drug or bioactive agent characterized with a positive surface charge and their enhanced permeability for paracellular protein drug and bioactive agent delivery.

Abstract: An ion source element includes a cold cathode, a grid electrode, and an ion accelerator. The cold cathode, the grid electrode, and the ion accelerator are arranged in that order and are electrically separated from one another. A space between the cold cathode and the grid electrode is essentially smaller than a mean free path of electrons at an operating pressure. The ion source element is thus stable and suitable for various applications.

Abstract: A system for monitoring compliance by a plurality of workers carrying wireless tags having worker identifiers with handwashing protocols in a facility having a plurality of areas. The system includes a plurality of wireless tag reader/transmitters having area identifiers for reading the worker identifiers stored in the wireless tags when the worker is positioned a predetermined distance from any of the plurality of wireless tag reader/transmitters and transmitting worker and area identifiers to at least one computing device; and at least one cleanser dispensing apparatus positioned in an area having at least one wireless tag reader/transmitter, the at least one cleanser dispensing apparatus alerting the at least one wireless tag reader/transmitter to read the worker identifiers and transmitting the worker and area identifiers.

Abstract: A frequency synthesizer. The frequency synthesizer comprises a harmonic locked phase/frequency detector, a low pass filter, a voltage controlled oscillator, and a frequency divider. The harmonic locked phase/frequency detector receives a reference signal and a divided signal. The low pass filter is coupled to the harmonic locked phase/frequency detector. The voltage controlled oscillator is coupled to the low pass filter and provides an output signal. The frequency divider is coupled between the voltage controlled oscillator and the harmonic locked phase/frequency detector. Frequency of the divided signal is a harmonic frequency of the reference signal.

Abstract: A family of structurally rigid dopamine D3 receptor selective ligands is described. The family of structurally rigid dopamine D3 receptor selective ligands has the formula wherein A is cis or trans —CH?CH—, —C?C—, or cyclohexyl. B is cis or trans —CH?CH— or absent. R1 represents an optionally substituted phenyl group, wherein said substituents are selected from the group consisting of: hydrogen, halogen, amino, nitro, hydroxyl, alkoxy, alkyl, acyl and pyridyl, and said substitution may occur at any of the ortho, meta, or para positions, or R1 represents a heteroaromatic ring. A preferred heteroaromatic ring is indole, quinoxoline, pyridyl, pyrimidyl, or imidazole. R2 and R3 may be independently hydrogen or a halogen, or R2 alone may be C1, C2, or C3 alkoxy, and m is 1 or 2, and n is 0, 1, or 2.

Abstract: An endopeptidase is described which shows specificity for cleavage of a LPXTG (SEQ ID NO: 1) motif found in the cell membranes of gram positive bacteria, having an apparent molecular weight of 14,000 daltons, having a pH optimum of about 7.5 to 10, being salt sensitive, being heavily glycosylated, rich in alanine, lacking aromatic amino acids, having a Km of 0.26 mM and having a backbone comprised of about 30% unknown amino acids, and containing carbohydrates that are essential for its activity. Methods of use and pharmaceutical compositions of this inhibitor are described.

Abstract: An exponential charge pump uses a number of identical or similar charging stages, each having a first and second capacitor. During a first clock phase, the first capacitor of each stage is charged by the second capacitor of the preceding stage, and, during a complementary second clock phase, the positive plate of the first capacitor of each stage is pushed to an increased voltage by the first capacitor of the preceding stage and charges the second capacitor of the next stage to the increased voltage at the same time. A similar mechanism occurs to the second capacitors in each stage, but with complementary timing. The increased voltage of the first capacitor of the last stage is pumped to an output capacitor during the second clock phase, and the increased voltage of the second capacitor of the last stage is pumped to an output capacitor during the first clock phase.

Abstract: A ferroelectric film includes a plurality of ferroelectric nanodomains configured in a regularly staggered fashion. The ferroelectric film has a quasi 2-dimensional configuration and is comprised of a ferroelectric material. A method for forming a ferroelectric film is also provided. A ferroelectric film comprised of a ferroelectric material is prepared. The ferroelectric film has a quasi 2-dimensional configuration and defines a direction that is normal to the quasi 2-dimensional configuration. An electric field along the normal direction is applied to the ferroelectric film, thereby the ferroelectric film having an array of ferroelectric nanodomains configured in a regularly staggered fashion is obtained.

Abstract: A method of stimulating homologous recombination by creating at least one nick in a targeted polynucleotide sequence. Wherein nonhomologous recombination is suppressed resulting in increasing the ratio of targeted to nontargeted events. A method of increasing double strand break-initiated gene targeting by inducing a nick in a targeted polynucleotide sequence, wherein overall recombination levels are increased. A method of increasing homologous recombination employing a recombinase that releases the ends in living cells by stimulating homolgous recombination to higher levels than those attainable with standard nucleases. A composition for stimulating homologous recombination including a nicking mechanism for creating nicks in a polynucleotide, wherein the nicking mechanism stimulates homologous recombination. A composition for stimulating homologous recombination including a nicking endonucleases. Various kits for stimulating homologous recombination.

Abstract: The present invention provides methods and compositions for enhancing memory in animals, including humans by the administration of an effective amount of an atypical form of protein kinase C such as protein kinase M zeta (PKM?) or protein kinase C iota/lambda.

Abstract: Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Abstract: The invention provides methods of screening for compounds that affect melanogenesis and the function of P protein in organisms, cells, or cell-free systems. The invention further relates to pharmacologic and cosmetic uses of methods of inhibiting melanogenesis, methods of activating melanogenesis, and compounds and pharmacologic compositions useful for the inhibition or activation of melanogenesis and, therefore, for lightening or darkening the pigmentation of cells and tissue, i.e., skin.

Abstract: Provided are a lossless audio coding/decoding apparatus and method. The lossless audio coding apparatus includes a first coder to directly code first symbols; a second coder module comprising a plurality of second coders to convert the first symbols into second symbols and to code the second symbols; a first selector to compare the performance of the first coder to the performance of the second coders and to output a coding mode in accordance with a comparison result; and a second selector to output a final bitstream by coding the first symbols in correspondence with the coding mode. According to the present invention, the performance of audio coding may be improved.

Abstract: The present teachings provide silole-based polymers that can be used as p-type semiconductors. More specifically, the present teachings provide polymers that include a repeating unit of Formula I: wherein R1, R2, R3, R4, R5, R6, Z, x, and x? are as defined herein. The present teachings also provide methods of preparing these polymers, and relate to various compositions, composites, and devices that incorporate these polymers.

Abstract: The present invention provides a method for diagnosing a subject suffering a pathological condition characterized by parvovirus infection by detecting the presence of IgE anti-Parvovirus B 19 antibodies.

Abstract: The present invention relates to a method of qualifying ovarian cancer status in a subject comprising: (a) measuring at least one biomarker in a sample from the subject and (b) correlating the measurement with ovarian cancer status. The invention further relates to kits for qualifying ovarian cancer status in a subject.

Abstract: Protein logic gates are made from autoregulated fusion proteins comprising an output domain and a plurality of input domains, wherein at least one of the input domains is heterologous to the output domain, and the input domains interact with each other to allosterically and external, ligand-dependently regulate the output domain. The output domain may be constitutively active, and in the absence of the ligand, the input domains interact to inhibit the output domain. The activity of the output domain is user discretionary, and may include activities that are catalytic, label-generative, metabolic-regulative, apototic, specific-binding, etc. Multiple input domains can cooperatively regulate the fusion protein in a wide variety of functionalities, including as an OR-gate, an AND-gate, and an AND-NOT-gate. The gates may be incorporated into cells and therein used to modulate cell function.

Abstract: The invention relates to a pharmaceutical composition for treating or diagnosing a disorder associated with production of peroxisome in a cell, comprising a polypeptide which has cysteine protease activity and directly processes peroxisomal enzymes targeted by PTS1 or PTS2 signals. Preferably, the polypeptide is encoded by Tysnd1.

Abstract: The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber drive capable of rotating the chamber at a first speed about a first axis and a second speed about a second axis, the second axis being disposed at an angle relative to the first axis. In use, the magnitude of the first speed and the second speed are independently variable of each other.

Abstract: A new apple variety distinguished by the following unique combination of characteristics: It is field-immune to apple scab disease, has good resistance to the bacterial disease fire blight, and moderate resistance to the fungal disease powdery mildew. This new apple is relatively late ripening. It has medium-large fruit size, red colored fruit, with excellent flavor (full favored, fruity, sweet, mild-subacid). The fruit hangs well on the tree, even after ripening. The tree is highly productive on an annual basis.