Abstract: The present invention relates to compositions and methods for detecting motor neuron-specific miRNAs in a population of cells or subject. More particularly, the invention relates to detecting motor neuron-specific miRNAs to detect and treat motor neuron diseases associated with dysregulation of motor neuron-specific miRNAs, such as Amyotrophic Lateral Sclerosis (ALS).

Abstract: T. gondii proteins MIC1 and MIC4 are components of excretory/secretory antigens (ESA) that elicit delayed type hypersensitivity (DTH) responses in infected animals. These antigens are capable of inducing IFN-g secretion by splenic T cells (ELISPOT assay), stimulating T cells to produce cytokines that recruit inflammatory monocytes and neutrophils resulting in a positive luminol test (luminol ear assay), and eliciting a positive skin test in the guinea pig.

Abstract: Due to the precautions put in place during the COVID-19 pandemic, utilization of telemedicine has increased quickly for patient care and clinical trials. Unfortunately, teleconsultation is closer to a video conference than a medical consultation with the current solutions setting the patient and doctor into a discussion that relies entirely on a two-dimensional view of each other. A telehealth platform is augmented by a digital twin of the patient that assists with diagnostic testing of ocular manifestations of myasthenia gravis. A hybrid algorithm combines deep learning with computer vision to give quantitative metrics of ptosis and ocular muscle fatigue leading to eyelid droop and diplopia. The system works both on a fixed image and video in real time allowing capture of the dynamic muscular weakness during the examination.

Abstract: Among the various aspects of the present disclosure is the provision of compositions for and methods of diagnosing, prognosing, and treating diabetes.

Abstract: The present invention provides methods for testing surgical fluid for biomarkers of disease, including cancer.

Abstract: Disclosed are methods and compositions related to methods of treating, ameliorating, mitigating, slowing, arresting, preventing or reversing various diseases and conditions, including age-related obesity, age-related increases in blood lipid levels, age-related decreases in insulin sensitivity, age-related decreases in memory function, and age-related changes in eye function such as macular degeneration. The methods comprise administering nicotinamide mononucleotide (NMN) to a subject. In some embodiments, the administration can be oral administration. Also disclosed are pharmaceutical compositions comprising NMN.

Abstract: A barbed rod device is provided for tissue closure and approximation. The device includes two or more subassemblies. Each subassembly includes a receiver having a central opening and centerline axis, a plurality of barbs extending radially outward from the receiver, and a linking rod extending from the receiver along the centerline axis of the receiver. The linking rod includes a locking end opposite the receiver. The locking end of a first subassembly is configured to engage the receiver of a second subassembly.

Abstract: Treatment and prevention of DNMT3A deficiency-associated diseases are provided. Compositions for treatment or prevention include at least one cDNA vector and/or at least one therapeutic agent capable of restoring DNMT3L activity, restoring DNMT3L expression, increasing DNMT3L activity, increasing DNMT3L expression, and/or increasing DNMT3A enzymatic activity. Methods for treatment or prevention include administering to a subject a composition including the at least one cDNA vector and/or at least one therapeutic agent. Methods of increasing DNMT3A activity in a subject having a DNMT3A mutation, methods of reversing a hypomethylation phenotype in bone marrow cells of a subject having a DNMT3A mutation, and methods of promoting cancer cell death in a subject having Acute Myeloid Leukemia (AML) are also provided.

Abstract: Transgenic strains of R. palustris TIE-1 and methods of use thereof for bioplastic production are provided. An exemplary transgenic R. palustris TIE-1 microorganism includes an exogenous RuBisCo ?rubI gene; and an exogenous RuBisCo ?rubII gene. An exemplary method of producing the bioplastic polyhydroxybutyrate (PHB) includes culturing a transgenic R. palustris TIE-1 microorganism in a culture medium, the transgenic R. palustris TIE-1 microorganism comprising an exogenous RuBisCo ?rubI gene; and an exogenous RuBisCo ?rubII gene; such that the exogenous RuBisCo ?rubI and ?rubII genes are overexpressed; and recovering the PHB from the culture medium.

Abstract: The present disclosure relates to biodegradable materials and methods of removing using the biodegradable materials to remove phosphorus from water. Additionally, the biodegradable materials may be used as a fertilizer.

Abstract: A multi-laminar electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The scaffold includes a first layer formed by a first plurality of electrospun polymeric fibers, and a second layer formed by a second plurality of electrospun polymeric fibers. The second layer is combined with the first layer. A first portion of the scaffold includes a higher density of fibers than a second portion of the scaffold, and the first portion has a higher tensile strength than the second portion. The scaffold is configured to degrade via hydrolysis after at least one of a predetermined time or an environmental condition. The scaffold is configured to be applied to the tissue substrate containing the defect, and is sufficiently flexible to facilitate application of the scaffold to uneven surfaces of the tissue substrate, and to enable movement of the scaffold by the tissue substrate.

Abstract: The present disclosure encompasses anti-TREM-2 antibodies that promote TREM-2 activation and methods of using the anti-TREM-2 agonist antibodies therapeutically.

Abstract: The present disclosure provides systems and methods for radio-luminescent imaging dosimetry of variable dose rate radiation beams at high spatial resolution. Exemplary embodiments include time-gating one or more camera shutters such that one image frame from a plastic scintillator detector (PSD) is captured per camera shutter for each radiation beam pulse produced from a pulsed beam accelerator directed toward the PSD.

Abstract: Among the various aspects of the present disclosure is the provision of methods for detecting cancer in Neurofibromatosis Type 1 (NF1) patients.

Abstract: System and methods for forming a micropillar array for an evaporative heat exchanger include selecting a preliminary shape for a micropillar, determining a droplet shape that is generated by the preliminary shape, and generating at least one curve that defines the droplet shape. The system and methods also include performing an evaporative simulation based on the curve and selecting a final micropillar shape based on the evaporative simulation. The system and methods further include fabricating an array of micropillars including at least one micropillar having the final micropillar shape.

Abstract: Compositions and methods of treating a thrombotic disease, disorder, or condition in a subject in need thereof are provided. Exemplary compositions comprise at least one ERO1? inhibitor. Exemplary methods comprise administering to the subject at least one ERO1? inhibitor. Methods of inhibiting ERO1? in a subject having a thrombotic disease, and methods of reducing platelet thrombus formation in subject having a thrombotic disease, are also provided.

Abstract: The present disclosure is generally directed to quinolinyl and pyridinyl containing compounds targeting on alpha-synuclein for neurological diseases. These compounds, particularly radiolabeled versions of these compounds, can be particularly useful in PET scans targeting diseases such as Parkinson's disease (PD), Dementia with Lewy Bodies (DLB) and Multiple System Atrophy (MSA).

Abstract: Methods and systems for (i) modulating, enhancing, or maintaining slow waves using phase-locked neurostimulation; (ii) modulating, enhancing, or maintaining unconsciousness using phase-locked neurostimulation; and (iii) reducing the amount of anesthesia required in a subject using phase-locked neurostimulation are provided. The present disclosure is directed to applying neurostimulation to the brain of a subject to enhance anesthesia, thereby decreasing drug requirements of anesthesia.

Abstract: Methods for generating blastoids from naïve hPSCs are provided. Exemplary methods include exposing the naïve hPSCs to 5i/L/A, aggregating the exposed hPSCs in N2B27, and treating the aggregated hPSCs with a simplified blastocyst induction medium (“BIM”). Also provided are systems for extended culture of human blastoids on a 3D matrix, including blastoids generated from naïve hPSCs according to methods described herein.

Abstract: The present disclosure provides method of treating or preventing a microbial infection caused by a prokaryotic pathogen in a subject, the method comprising administering a compound of Formula (I) as set forth in the specification. In one embodiment, the prokaryotic pathogen belongs to the genus Staphylococcus.