Abstract: Described herein are systems using injectionless gel electrophoresis (GE), such as thermal GE (TGE), to selectively separate, concentrate, quantify, and/or otherwise analyze target analytes. Inline preconcentration and separation are demonstrated to resolve analytes, exemplified by resolving double-stranded miRNA-probe hybrids from excess single-stranded probes and analyzing multiple conformations of a protein. Microfluidic devices having a tapered channel are described, which improve detection sensitivity and separation resolution. The described separation strategy and microfluidic device designs establish injectionless gel electrophoresis as a simple, low-cost analysis method, for instance for analyzing clinical and pharmaceutical samples, including for miRNA, protein, and other biomolecular analyses.

Abstract: A detection layer (416) for a radiation detector (400) includes a porous silicon membrane (418). The porous silicon membrane includes silicon (419) with a first side (430) and a second opposing side (432), a plurality of pores (420) extending entirely through the silicon from the first side to the second opposing side, each including shared walls (426), at least one protrusion of silicon (424) protruding out and extending from the first side a distance (504, 604, 704). The porous silicon membrane further includes a plurality of radiation sensitive quantum dots (422) in the pores and a quantum dot layer disposed on the first side and having a surface (434) and a thickness (506, 606, 706), wherein the thickness is greater than the distance.

Abstract: Described herein are systems and methods for modeling fluid flow. The systems may comprise a plurality of chambers, a plurality of sensors, a pump, a plurality of valves, and a flow limiting operator (FLO). The respective sensor of the plurality of sensors may be configured to sense a respective monitoring parameter associated with the respective chamber. The pump may be configured to move media to or from the respective chamber. The respective valve of the plurality of valves may be configured to control a fluid velocity of the media flowing through the respective chamber. The FLO may be electrically connected to the plurality of sensors, the pump, and the plurality of valves. The FLO may be configured to independently control the respective chamber by manipulating one or more controllable parameters associated with the respective chamber based on the respective monitoring parameter associated with the respective chamber.

Abstract: Various systems, methods, and devices for identifying instrument movements likely to cause intraoperative bleeding are described. An example method includes identifying a movement of an instrument; determining that the movement of the instrument exceeds a threshold; and dampening the movement of the instrument based on determining that the movement exceeds the threshold.

Abstract: Molybdenum-DAD precursors are described. Methods for depositing molybdenum-containing films on a substrate are described. The substrate is exposed to a molybdenum-DAD precursor and a reactant to form the molybdenum-containing film (e.g., elemental molybdenum, molybdenum oxide, molybdenum carbide, molybdenum silicide, molybdenum nitride). The exposures can be sequential or simultaneous.

Abstract: Apparatuses, systems, and methods for Raman spectroscopy are described. In certain implementations, a spectrometer is provided. The spectrometer may include a plurality of optical elements, comprising an entrance aperture, a collimating element, a volume phase holographic grating, a focusing element, and a detector array. The plurality of optical elements are configured to transfer the light beam from the entrance aperture to the detector array with a high transfer efficiency over a preselected spectral band.

Abstract: A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

Abstract: Dendrimer-based compositions and methods are provided, that are useful for administering pharmaceutical compositions to target cells and tissues for treatment of ocular diseases including macular degeneration, diabetic retinopathy, and retinitis pigmentosa.

Abstract: Provided are systems and methods for assessing the presence or risk of obstetrical complications, particularly those related to an angiogenic and anti-angiogenic imbalance. Also provided are methods of treating an angiogenic and anti-angiogenic imbalance with water-soluble statins, such as pravastatin.

Abstract: An exemplary system may include an augmented reality device with a user interface, a processor and memory having a program communicatively connected to the processor. A transceiver may be communicatively connected to a practitioner device for real-time practitioner guidance. The system may include a plurality of sensors and a feedback device in communication with the processor. The system may display initial and updated guidance objects in a real-time environment of the user and in response to sensor outputs of the plurality of sensors. The feedback device may provide at least one of audio, visual, and tactile feedback in response to the sensors outputs. The system may condition the user to provide a desired reaction by guiding the real-time user reactions in the real-time environment using the feedback in response to the sensor outputs.

Abstract: Methods for in vivo immunoimaging including: (a) administering a labeled-antibody conjugate to a subject, wherein the labeled-antibody conjugate includes: an antibody that specifically recognizes and binds to IFN-?, and a detection label conjugated to the antibody, wherein the detection label is a radionuclide tracer or tluorophore; and (b) detecting the presence of the radiolabeled-antibody conjugate in the subject in vivo by imaging. Embodiments of the present disclosure are directed to labeled-antibody conjugates and therapeutic radionuclide-antibody conjugates.

Abstract: A ring oscillator includes a first set of at least three laddered inverter quantizer (LIQAF) circuits connected in stages that are in series, including a first LIQAF circuit and a last LIQAF circuit, and a feedback circuit from the last LIQAF circuit to the first LIQAF circuit having a logical NOT output compared to the first LIQAF circuit. A voltage input creates a pair of phase shifted waveforms in the first of the at least three LIQAF circuits that propagate sequentially through the stages of the at least three LIQAF circuits. Each stage has a pair of outputs to the next stage that are then phase shifted from the previous stage in the next stage.

Abstract: A radiation detector (100) includes a photovoltaic layer (102) with first and second opposing sides. The photovoltaic layer is configured to absorb first radiation at the first side and produce electrical charge. The detector further includes a porous silicon quantum dot layer (104) disposed at the second side of the photovoltaic layer and configured to receive second radiation and convert the received second radiation into an electrical signal indicative of an energy level of the received second radiation. The detector further includes an acquisition and communication layer (106) disposed adjacent to the porous silicon quantum dot layer and configured to receive the electrical signal and transmit the electrical signal to a device remote from the radiation detector.

Abstract: The present invention relates to compositions and methods for enhancing T cell metabolism and activity for more effective adoptive T cell therapy. By expressing an intracellular signaling molecule in T cells, the T cells are metabolically enhanced with improved cytotoxicity and resistance to immunosuppression imposed by tumor microenvironments. One aspect includes a modified T cell and pharmaceutical compositions comprising the modified cells for adoptive cell therapy and treating a disease or condition associated with enhanced immunity.

Abstract: Nanoparticle assemblies can be formed and deposited on temperature sensors to create low cost, high sensitivity hydrogen sensors. The nanoparticle assemblies can be formed from nanoparticles within an electrolysis reactor to form grain-boundary connections between the nanoparticles of the nanoparticle assembly. The nanoparticle assembly can be deposited on temperature sensors to create hydrogen gas sensors.

Abstract: Described herein are decellularized extracellular matrix for mechanically supporting engineered vascular grafts. Methods are provided for fabricating all-natural, non-immunogenic, strong products that do not rely on common plastic supports. Also provided are bench-top models of atherosclerosis. Embodiments provide completely inclusive models that contain all steps of atherosclerosis, including late-stage disease processes. Example models utilize tissue engineered blood vessels (TEBV); stages of atherosclerosis are induced for instance by application of oxidized low-density lipoprotein (oxLDLs) (early-stage), followed by macrophage introduction (early-stage), and induction of calcification using calcified protein particles (CPPs; late-stage). Also provided are kits useful to investigate disease processes and better patient treatment options, including new drug development.

Abstract: Amphiphilic oligonucleotide conjugates that enhance adjuvant function are disclosed. The conjugates typically include: a lipophilic component, and conjugated thereto (directly or indirectly) an immunomodulating oligonucleotide that, if it were not conjugated to the lipophilic component, would suppress TLR7 and/or TLR8 stimulation. In the presence of albumin, these conjugates significantly enhance adjuvant function, in particular the function of TLR7/8-mediated adjuvants such as an imidazoquinolinamine. The conjugates can be administered, along with an adjuvant compound, to a subject in order to cause and/or enhance an immune response (for instance, to an infectious agent or a cancer antigen) in the subject.

Abstract: Anti-heparanase compounds for the treatment of diabetes are described. The anti-heparanase compounds are high affinity, synthetic glycopolymers that result in minimal anticoagulant activity. Stereoselective fluorinated forms of these compounds are also provided.

Abstract: The current disclosure provides alternating current based systems and methods to develop chemical compounds, such as drug molecules using electrochemistry in organic synthesis.

Abstract: A four-level rectifier may include an output, a first capacitor, a second capacitor, a third capacitor, and three phases. The first, second, and third capacitors may be connected in series. The output may be connected between the first capacitor and the third capacitor. Each of the three phases may include an input, a first diode, a second diode, a first switch, a second switch, and a third switch. The first diode may be connected between the input and the first capacitor. The second diode may be connected between the input and the third capacitor. The first switch may be connected between the input and the second switch and the third switch. The second switch may be connected to the first capacitor and to the second capacitor. The third switch may be connected to the second capacitor and to the third capacitor.