Abstract: Disclosed herein are in vivo engineered cereblon protein and methods of making the same. The in vivo engineered cereblon protein can include a site-specific non-naturally occurring modification at cysteine 287 as set forth in SEQ ID NO:1, or cysteine 286 as set forth in SEQ ID NO:2 or 3, the modification comprising a moiety resulting from an in vivo Michael addition reaction between an exogenous Michael acceptor and the cysteine 287 as set forth in SEQ ID NO:1, or cysteine 286 as set forth in SEQ ID NO:2 or 3.
Type:
Grant
Filed:
August 8, 2019
Date of Patent:
September 22, 2020
Assignees:
VIVIDION THERAPEUTICS, INC., THE SCRIPPS RESEARCH INSTITUTE
Inventors:
David Weinstein, Benjamin F. Cravatt, Matthew Patricelli, Dean Stamos, Gabe Simon, Benjamin Horning, Ekaterina Vinogradova, Brian Nordin, Kristen Baltgalvis, Todd Kinsella
Abstract: The present invention relates to albumin variants with an improved affinity for the neonatal Fc receptor (FcRn) and uses thereof, and in particular to the use of such albumin variants as carriers for immunogens. In some embodiments, the present invention relates to vaccines (e.g., vaccines for mucosal delivery) comprising albumin/immunogen fusion proteins.
Type:
Grant
Filed:
January 17, 2019
Date of Patent:
September 22, 2020
Assignee:
University of Oslo
Inventors:
Jan Terje Andersen, Inger Sandlie, Malin Bern
Abstract: Detergent compositions comprising polypeptide variants and methods of cleaning and/or treatment of surfaces using such compositions, and fabric treatment compositions comprising polypeptide variants. The compositions may comprise surfactants: anionic, nonionic and/or cationic surfactants.
Type:
Grant
Filed:
October 26, 2018
Date of Patent:
September 22, 2020
Assignee:
The Procter & Gamble Company
Inventors:
Lars Henrik Oestergaard, Lars Giger, Annette Helle Johansen, Lars Beier, Jürgen Carsten Franz Knötzel, Gernot J. Abel, Klaus Gori, Neil Joseph Lant
Abstract: The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
July 12, 2017
Date of Patent:
September 15, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Lars Beier, Lars Henrik Oestergaard, Annette Helle Johansen, Klaus Gori, Thomas Holberg Blicher, Gernot Abel, Steen Krogsgaard, Jens E. Nielsen, Lars Giger
Abstract: The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3?-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.
Type:
Grant
Filed:
October 19, 2018
Date of Patent:
September 15, 2020
Assignee:
Molecular Assemblies, Inc.
Inventors:
J. William Efcavitch, Julie L. Tubbs, Prem Sinha, Boguslaw Stec
Abstract: The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3?-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.
Type:
Grant
Filed:
August 27, 2018
Date of Patent:
September 1, 2020
Assignee:
Molecular Assemblies, Inc.
Inventors:
J. William Efcavitch, Julie L. Tubbs, Prem Sinha, Boguslaw Stec
Abstract: The invention relates to a hybrid structure comprising perforated solid substrate having at least one nanopore perforating therethrough, and devices and uses thereof.
Type:
Grant
Filed:
December 10, 2018
Date of Patent:
September 1, 2020
Assignee:
Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
Inventors:
Danny Porath, Oded Shoseyov, Yuval Nevo, Ke Liu, Dvir Marom Rotem
Abstract: This disclosure provides streptavidin monomers that bind biotin with high affinity and which possess slow dissociation rates. The disclosure also provides methods of making and using those monomers.
Type:
Grant
Filed:
July 25, 2017
Date of Patent:
September 1, 2020
Assignee:
The Research Foundation for The State University of New York
Abstract: The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.
Type:
Grant
Filed:
May 17, 2017
Date of Patent:
August 25, 2020
Inventors:
John Leite, Robert Pope, Mahbod Hajivandi, Xiquan Liang
Abstract: The present invention relates to a composition comprising: (a) at least one surfactant, at least one surfactant system, at least one soap, or any mixtures thereof; and (b) a polypeptide having lipase activity selected from the group consisting of: (I) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2; (II) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i); (III) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; (IV) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and (V) a fragment of the polypeptide of (I), (II), (III), or (IV); wherein said composition is a cleaning and/or treatment composition.
Type:
Grant
Filed:
December 18, 2014
Date of Patent:
August 4, 2020
Assignee:
Novozymes A/S
Inventors:
Marc Dominique Morant, Anna Verena Reiser, Carsten Hoerslev Hansen, Kim Borch, Lone Baunsgaard, Vikram Kjoeller Bhatia
Abstract: The present invention relates to a novel use of ?-1,3-1,6-endoglucanase producing oligosaccharides or glucose from ?-glucan. More specifically, the present invention provides an effect of producing oligosaccharides or glucose of various degrees of polymerization in high yields by using a ?-1,3-1,6-endoglucanase exhibiting ?-1,3-endoglucanase and ??-1,6-endoglucanase activity on ?-glucan and exhibiting transglycosylation activity on laminarioligosaccharide.
Type:
Grant
Filed:
April 28, 2017
Date of Patent:
July 28, 2020
Assignee:
Korea University Research and Business Foundation
Inventors:
Kyoung Heon Kim, Damao Wang, Do-Hyoung Kim
Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Type:
Grant
Filed:
May 5, 2017
Date of Patent:
July 28, 2020
Assignee:
Codexis, Inc.
Inventors:
Jovana Nazor, Vesna Mitchell, David Elgart, Katrina W. Lexa, Nikki Dellas, Robert Kevin Orr, Oscar Alvizo, Ravi David Garcia, Judy Victoria Antonio Viduya, Courtney Dianne Moffett
Abstract: The present invention relates to a method for storing gaseous hydrogen, comprising the steps of producing methanoate (formate) through contacting gaseous hydrogen with carbon dioxide in the presence of a hydrogen dependent carbon dioxide reductase (HDCR), and thereby storing of said gaseous hydrogen. The HDCR and/or its complex is preferably derived from Acetobacterium woodii.
Abstract: Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.
Abstract: Provided herein are methods shifting the isoelectric profile of a recombinant protein product and the use of such methods in the manufacture of recombinant protein products. Also provided are recombinant protein products produced by such methods.
Type:
Grant
Filed:
January 19, 2018
Date of Patent:
July 14, 2020
Assignee:
Alexion Pharmaceuticals, Inc.
Inventors:
Hunter F. Malanson, Pratik Jaluria, Rachael Alford
Abstract: An object of the present invention is to provide an albumin-sugar chain complex bound with a number of sugar chains sufficient for obtaining a sugar chain clustering effect while also being able to exist comparatively stably in the body. The present invention provides an albumin-sugar chain complex having five or more molecules of an asparagine-linked sugar chain bound per molecule of albumin; a carrier for selectively delivering a functional molecule to a target tissue in the body that contains the aforementioned albumin-sugar chain complex; and a bioimaging probe having the aforementioned albumin-sugar chain complex as an active ingredient thereof that is administered into the body of an animal.
Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for detecting anti-carbamylated protein (anti-CarP) antibodies in the serum of rheumatoid arthritis (RA) patients.
Type:
Grant
Filed:
October 23, 2017
Date of Patent:
June 16, 2020
Assignees:
Inova Diagnostics, Inc., Leiden University Medical Center
Inventors:
Michael Mahler, Leendert A. Trouw, Jan Wouter Drijfhout, Tom Huizinga, René Toes, Peter Van Veelen