Patents Examined by Anand U Desai
  • Compositions comprising digestive enzymes
    Patent number: 10927360
    Abstract: Provided herein are compositions with enhanced protein specific activity, protein combinations and methods for the preparation thereof.
    Type: Grant
    Filed: June 3, 2020
    Date of Patent: February 23, 2021
    Assignee: CLARA FOODS CO.
    Inventors: Halle Redfearn, Harshal Kshirsagar, Kritika Mahadevan, Alexandre Chapeaux, Wesley Rutherford-Jenkins
  • Obtaining high-performance yeast strains for metabolizing arabinose
    Patent number: 10920232
    Abstract: The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: February 16, 2021
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Thomas Desfougeres, Emilie Fritsch, Georges Pignede, Christophe Rave, Claire Thorel
  • Formation of stable submicron peptide or protein particles by thin film freezing
    Patent number: 10898437
    Abstract: The present invention includes compositions and methods for preparing micron-sized or submicron-sized particles by dissolving a water soluble effective ingredient in one or more solvents; spraying or dripping droplets solvent such that the effective ingredient is exposed to a vapor-liquid interface of less than 50, 100, 150, 200, 250, 200, 400 or 500 cm?1 area/volume to, e.g., increase protein stability; and contacting the droplet with a freezing surface that has a temperature differential of at least 30° C. between the droplet and the surface, wherein the surface freezes the droplet into a thin film with a thickness of less than 500 micrometers and a surface area to volume between 25 to 500 cm?1.
    Type: Grant
    Filed: May 13, 2019
    Date of Patent: January 26, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Keith P. Johnston, Joshua Engstrom, Robert O. Williams, III
  • Production method for insoluble recombinant protein aggregate
    Patent number: 10899792
    Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: January 26, 2021
    Assignee: Spiber Inc.
    Inventors: Toshiaki Osawa, Yuya Sato, Keisuke Morita
  • Methods for generating engineered enzymes
    Patent number: 10900059
    Abstract: Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., “cleaveOme”) inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: January 26, 2021
    Assignee: Research Development Foundation
    Inventors: Brent Iverson, Peter Marek, Joseph Taft
  • Method for the production of pullulan soft capsules
    Patent number: 10894971
    Abstract: A method for the production of pullulan soft capsules eliminates the need to dry pullulan solid product, thereby reducing the equipment cost and energy consumption. The pullulan raw material production can be linked directly with the soft capsule production to provide a unique approach for soft capsule formation. The purified pullulan fermentation fluid can be directly used in soft capsule preparation, thus removing the need for a melting process. On the one hand, the method can decrease material consumption, save the cost of equipment and labor, reduce production time and increase productivity. On the other hand, the method can reduce the fluctuating of raw material quality in the re-melting process and guarantee a more stable soft capsule production and quality.
    Type: Grant
    Filed: June 26, 2019
    Date of Patent: January 19, 2021
    Assignee: LEFAN CAPSULE INTERNATIONAL INC
    Inventors: Xianyu Yun, Sheng Wang, Zhongming Fang, Fei Wang, Peiyong Liu, Xiaosan Cao, Zhidong Lu
  • Methods and products for in vivo enzyme profiling
    Patent number: 10883998
    Abstract: The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: January 5, 2021
    Assignee: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
  • Biological upgrading of hydrocarbon streams with oxygenases
    Patent number: 10883088
    Abstract: Oxygenases and methods of biologically upgrading hydrocarbon streams, such as crude oil, using oxygenases are provided herein. The oxygenases can be used to remove impurities such as metals, heteroatoms, or asphaltenes from a hydrocarbon stream. In some cases, the oxygenases can be chemically or genetically modified and can be used in different locations such as petroleum wells, pipes, reservoirs, tanks and/or reactors.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: January 5, 2021
    Assignee: ExxonMobil Research and Engineering Company
    Inventors: Zarath M. Summers, David O. Marler, Jay B. Patel, Katherine G. Landuyt
  • DNA polymerase variant and application thereof
    Patent number: 10883091
    Abstract: Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.
    Type: Grant
    Filed: July 8, 2019
    Date of Patent: January 5, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Lin Wang, Fen Liu, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac
  • Method for producing polyhydroxyalkanoic acid, and microbes
    Patent number: 10876140
    Abstract: Provided is a method for producing PHA by a microbe with improved productivity of PHA, and a PHA-producing microbe used for the production method. A method for producing polyhydroxyalkanoic acid, the method including a step of culturing a microbe having a polyhydroxyalkanoic acid synthase gene and an inactivated gene encoding a flagellar protein to cause the microbe to produce polyhydroxyalkanoic acid. In the microbe, a lipase, a dephosphorylating enzyme, and a protein represented by the amino acid sequence of SEQ ID NO: 6 or 7 may be additionally inactivated. The microbe may be Cupriavidus necator.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: December 29, 2020
    Assignee: KANEKA CORPORATION
    Inventors: Shunsuke Sato, Hisashi Arikawa, Rina Aoki
  • Method for producing objective substance
    Patent number: 10858676
    Abstract: A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so as to have a specific feature, such as a reduced activity of AICAR formyltransferase/IMP cyclohydrolase, an increased activity of 3-PGDH, and/or a reduced activity of L-serine deaminase.
    Type: Grant
    Filed: May 17, 2018
    Date of Patent: December 8, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Christine Roche, Benjamin Mijts, Peter Kelly, Matthew Davis, Sayaka Asari, Keita Fukui, Miku Toyazaki
  • Method and apparatus for microneedle transdermal delivery
    Patent number: 10849962
    Abstract: A method for forming microspheres containing bioactive material, comprising dissolving a polymer matrix, such as albumin or beta-cyclodextrin, in an aqueous medium in a first vessel; contacting the dissolved polymer matrix with a crosslinking agent, such as glutaraldehyde, to crosslink the polymer matrix and the crosslinking agent; neutralizing with sodium bisulfate any excess crosslinking agent remaining after crosslinking is substantially complete; solubilizing in a second vessel a bioactive material in an aqueous solution; mixing the solubilized bioactive material together with the neutralized crosslinked polymer matrix in solution to form a mixture; and, spray drying the mixture to produce nanospheres, whereby substantial bioactivity of the biomaterial is retained upon cellular uptake.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: December 1, 2020
    Assignee: THE CORPORATION OF MERCER UNIVERSITY
    Inventor: Martin J. D'Souza
  • Plant-derived elastin binding protein ligands and methods of using the same
    Patent number: 10844101
    Abstract: The present invention describes novel plant derived elastin-like peptides and peptidomimetics that may serve as functional ligands for elastin receptors and stimulate elastogenesis. The novel plant derived peptides provide an alternative (non-animal derived) source of GXXPG (SEQ ID NO. 2) containing peptides. The present invention also describes therapeutic compositions containing novel plant derived peptides or peptidomimetics useful in stimulating elastogenensis and capillary dilatation. The therapeutic compositions of the present invention that comprise novel plant derived peptides or peptidomimetics may be combined with other therapeutic agents.
    Type: Grant
    Filed: November 27, 2018
    Date of Patent: November 24, 2020
    Assignees: Elastogenesis, LLC, The Hospital for Sick Children
    Inventors: Felipe Jimenez, Thomas Mitts, Aleksander Hinek
  • Coatings and biomedical implants formed from keratin biomaterials
    Patent number: 10821211
    Abstract: Methods are provided to produce optimal fractionations of charged keratins that have superior biomedical activity. Also provided are medical implants coated with these keratin preparations. Further provided are methods of treating blood coagulation in a patient in need thereof.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: November 3, 2020
    Assignee: Wake Forest University Health Sciences
    Inventor: Mark E. Van Dyke
  • DNA repair skin care composition
    Patent number: 10821180
    Abstract: Disclosed is a method of preventing or treating a condition of the gastrointestinal tract. The method includes the steps of providing a DNA repair composition, the DNA repair composition comprising at least one DNA repair enzyme, the DNA repair composition configured for administration within the gastrointestinal tract of a patient; and administering the DNA repair composition to the patient, such that the DNA repair composition is absorbed within the gastrointestinal tract of the patient to treat the condition of the gastrointestinal tract.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: November 3, 2020
    Inventor: Ronald L. Moy
  • Chemical crosslinkers
    Patent number: 10814008
    Abstract: Disclosed herein are methods of chemical conjugation comprising contacting a lysosomal enzyme with a first crosslinking agent to introduce aldehyde groups; contacting a lysosomal targeting peptide with a second crosslinking agent to introduce a hydrazide group at the N-terminal residue; contacting the lysosomal enzyme with aldehyde groups of step a. with the lysosomal targeting peptide with a hydrazide group at the N-terminal residue of step b; and forming a lysosomal enzyme-lysosomal targeting peptide conjugate.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: October 27, 2020
    Assignee: Amicus Therapeutics, Inc.
    Inventor: Hung Do
  • Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
    Patent number: 10808269
    Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: October 20, 2020
    Assignee: DANISCO US INC
    Inventors: Zheyong Yu, Slavko Kralj, Zhenghong Zhang, Laurie A. Howe
  • Detergent compositions comprising polypeptide variants
    Patent number: 10800997
    Abstract: Detergent compositions comprising polypeptide variants and methods of cleaning and/or treatment of surfaces using such compositions, and fabric treatment compositions comprising polypeptide variants. The compositions may comprise surfactants: anionic, nonionic and/or cationic.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: October 13, 2020
    Assignee: The Procter & Gamble Company
    Inventors: Lars Beier, Klaus Gori, Lars Henrik Oestergaard, Lars Giger, Annette Helle Johansen, Gernot J. Abel, Neil Joseph Lant
  • Methods and materials for making simvastatin and related compounds
    Patent number: 10793884
    Abstract: The invention disclosed herein relates to methods and materials for producing simvastatin and related compounds such as huvastatin.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: October 6, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Yi Tang, Xinkai Xie
  • Oral dissolving films
    Patent number: 10786558
    Abstract: A method for forming microspheres containing bioactive material, comprising dissolving a polymer matrix, such as albumin or beta-cyclodextrin, in an aqueous medium in a first vessel; contacting the dissolved polymer matrix with a crosslinking agent, such as glutaraldehyde, to crosslink the polymer matrix and the crosslinking agent; neutralizing with sodium bisulfate any excess crosslinking agent remaining after crosslinking is substantially complete; solubilizing in a second vessel a bioactive material in an aqueous solution; mixing the solubilized bioactive material together with the neutralized crosslinked polymer matrix in solution to form a mixture; and, spray drying the mixture to produce nanospheres, whereby substantial bioactivity of the biomaterial is retained upon cellular uptake.
    Type: Grant
    Filed: June 25, 2018
    Date of Patent: September 29, 2020
    Assignee: THE CORPORATION OF MERCER UNIVERSITY
    Inventor: Martin J. D'Souza