Patents Examined by Blaine Lankford
  • Patent number: 10466156
    Abstract: A method is provided for transporting a plurality of cells through a flow chamber, wherein the cells are initially immobilized on an internal surface of the flow chamber. The method comprises: selectively releasing the cells from the internal surface of the flow chamber; and flowing liquid through the flow chamber such that the released cells travel with the liquid, thereby transporting the cells through the flow chamber. Cells can be immobilized on or selectively released from the internal surface by applying or removing a trapping potential. The trapping potential can arise from an electric field gradient or an optical field gradient. Alternatively, cells can be selectively released from the surface using photocatalysis. Selective release allows cells to be individually observed or analyzed downstream, and can be based on a signal detected from one or more cells immobilized on the surface.
    Type: Grant
    Filed: August 19, 2015
    Date of Patent: November 5, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Daniel Y. Chu, Paul J. Patt, Roger Tong
  • Patent number: 10456785
    Abstract: An optical sensor and airborne pathogen proliferation assembly for remote, optical detection and monitoring of pathogens is disclosed.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: October 29, 2019
    Inventors: Stephen Daniels, Shane Phelan, Ruairi Monaghan
  • Patent number: 10450540
    Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 22, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
  • Patent number: 10449221
    Abstract: Embodiments herein relate in vitro methods of stem cell differentiation into thyroid hormone producing cells and tissues, and methods of use of these cells.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: October 22, 2019
    Inventors: Darrell Kotton, Maria Serra, Anthony N. Hollenberg, Anita A. Kurmann
  • Patent number: 10444126
    Abstract: Methods are provided for directly converting histopathologically processed biological samples, tissues, and cells into a multiuse biomolecule lysate. This method allows for simultaneous extraction, isolation, solubilization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in biochemical assays.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: October 15, 2019
    Inventors: Marlene M. Darfler, David B. Krizman
  • Patent number: 10440972
    Abstract: The present invention relates to a liquid milk fortifier composition for administration to infants, which fortifier composition comprises lipids in an amount of above 75% of the caloric content. The fortifier may be administrated to infants either as a supplement to human breast milk or to infant formulas. The present invention also relates to the use of the fortifier composition as well as a kit of parts for use in providing a lipid and/or calorie adapted body weight dependent nutrition to infants.
    Type: Grant
    Filed: November 29, 2013
    Date of Patent: October 15, 2019
    Assignee: Societe des Produits Nestle S.A.
    Inventors: Laurent Ameye, Marilisa Hamaoka-Hermann, Simona Stan, Sze Tan
  • Patent number: 10435658
    Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: October 8, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones
  • Patent number: 10434131
    Abstract: Compositions comprising apple, grape, green tea, and olive extracts are presented herein. This synergistic formulations apple, grape, green tea, and olive extract are in amounts that provide a greater antioxidant activity or protein kinase modulating activity than provided by an equivalent amount of any one extract or a sum of the extracts. Further presented are methods of regulating oxidative stress, disease-associated protein kinase activity, and enhancing the therapeutic effect of a primary therapeutic agent. Also presented are methods of making an activity enhancing composition for regulating oxidative stress, disease-associated protein kinase activity, and enhancing the therapeutic effect of a primary therapeutic agent.
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: October 8, 2019
    Assignee: Nature's Sunshine Products, Inc.
    Inventors: Matthew L. Tripp, Clinton J. Dahlberg, John G. Babish, Wei Gao, Mohan Kaadige, Sheryl Krig, Xiaolan Kou, Zhe Khang, Joseph Ou
  • Patent number: 10420856
    Abstract: Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: September 24, 2019
    Inventors: Treena Arinzeh, George Collins, Yee-Shuan Lee
  • Patent number: 10413588
    Abstract: The present disclosure provides for methods providing a pre/probiotic agent to a subject. Human Intelectin 1 (hIntL-1) has been shown to bind selectively to glycan components on bacteria, thereby promoting and protecting the microbiome.
    Type: Grant
    Filed: November 3, 2016
    Date of Patent: September 17, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Laura L. Kiessling, Darryl A. Wesener, Federico Rey, Kittikhun Wangkanont
  • Patent number: 10400212
    Abstract: This invention concerns methods of packaging and shipping stem cells. Also disclosed are related package products.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: September 3, 2019
    Assignee: Stemcyte Inc.
    Inventors: Wise Young, Dongming Sun, Robert Fleischaker, Kam Sze Kent Tsang
  • Patent number: 10385313
    Abstract: Described herein are cells, cell culture methods, and cell culture media compositions useful for producing and maintaining iPSC-derived cell lines that are of higher purity and maintain cell type integrity better than current iPSC-derived cell lines. Also disclosed are methods of using the described cells and media, such as therapeutic methods of use for the described cells. The described cells include iPSC-derived mesodermal precursor cells (MPC), which itself may differentiate into at least four different cell types. When cultured under appropriate conditions, the mesodermal precursor cells can be used to produce hematopoietic stem cells (HSC), mesenchymal stem cells (MSC), smooth muscle cells (SMC), or unlimited functional endothelial cells (UFEC). One characteristic that makes the described cells desirable is that they can be maintained in culture for a number of days, or passages, without changing phenotype through differentiation.
    Type: Grant
    Filed: October 1, 2014
    Date of Patent: August 20, 2019
    Assignee: The USA, as represented by the Secretary, Department of Health and Human Services
    Inventors: Manfred Boehm, Guibin Chen, Mahendra Rao, Andre Larochelle
  • Patent number: 10385311
    Abstract: A method of preparing a biological composite matrix system includes combining tissue microparticles with a curable medium. The tissue microparticles are formed by pulverizing tissue, sorting the pulverized tissue particles, and decellularizing the pulverized tissue particles. The tissue microparticles are then mixed with the curable medium and the resulting composite is cured to form a microparticle-medium composite matrix. The microparticle-medium composite matrix is compressed to increase the strength of the matrix and to increase the density of the microparticles to a predetermined density.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: August 20, 2019
    Assignee: Purdue Research Foundation
    Inventors: Corey Philip Neu, Tyler Anthony Novak
  • Patent number: 10378028
    Abstract: The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, E. harbinense YUAN-3, and a facultative anaerobic bacterium, P. aeruginosa PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth of E. harbinense YUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: August 13, 2019
    Assignee: Harbin Institute of Technology
    Inventors: Defeng Xing, Najiaowa Yu, Wei Li, Zhen Li, Yang Yang
  • Patent number: 10377987
    Abstract: A method for preparing a leukocyte preparation from a leukocyte fraction and a correspondingly prepared or obtainable leukocyte preparation and its use. The method includes: a) sedimenting the leukocyte fraction removing leukocyte supernatant from the sediment, wherein the leukocyte supernatant is collected or remains in a leukocyte container, b) sedimenting the leukocytes in the leukocyte container, c) washing the sediment in the leukocyte container with a saline solution, and d) resuspending the sediment in the leukocyte container in a storage solution.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: August 13, 2019
    Assignees: Artcline GmbH, Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V.
    Inventors: Jens Altrichter, Steffen Mitzner, Antje Lübcke, Kathleen Selleng, Fanny Doss, Stephanie Koch
  • Patent number: 10363279
    Abstract: An embodiment is a method of preventing, mitigating or treating neurological conditions and diseases that includes administering an effective amount of a medicament comprised of Lactobacillus bulgaricus B-30892 and/or a supernatant resulting from culturing Lactobacillus bulgaricus B-30892 and/or bioactive materials resulting from culturing Lactobacillus bulgaricus B-30892 to a human to prevent, mitigate or treat neurological conditions and diseases.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: July 30, 2019
    Assignee: NuBiome, Inc.
    Inventor: Brian C. Lue
  • Patent number: 10363276
    Abstract: Described are compositions and methods for treating liver disease, e.g., acute liver disease, using bone marrow-derived stem cells and bone marrow-derived stem cell conditioned media.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: July 30, 2019
    Assignees: The General Hospital Corporation, Massachusetts Institute of Technology
    Inventors: Martin Leon Yarmush, Biju Parekkadan
  • Patent number: 10351818
    Abstract: The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or “LBH”) which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds. LBH can be used as a substrate to promote the fermentation-based production of biobased industrial chemicals or biofuels, LBH can be utilized as a prebiotic to promote the growth of beneficial probiotic organisms. In addition, LBH may also be useful in stimulating the fermentation-based production of other products, examples of which include preservatives, antibiotics, antigens, vaccines, amino acids, vitamins, recombinant proteins, bioremediation treatments, and immobilized enzymes.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: July 16, 2019
    Assignees: LeafPro, LLC, University of Maryland, College Park
    Inventors: Yangming Martin Lo, Irene N. N. Yossa, Neil A. Belson
  • Patent number: 10335437
    Abstract: Provided are methods, pharmaceutical compositions, and kits for treatment of a metabolic condition, including obesity and type 2 diabetes by administration of a therapeutically effective amount of a cell or tissue preparation such as brown adipose microtissues to a subject, where the microtissues comprise adipose stem cells and endothelial cells. Modified approaches to creating brown adipose tissue involve differentiation of explanted white adipose tissue rather than isolation and expansion of adipose stems cells or endothelial cells and formation and differentiation of 3D cell aggregates.
    Type: Grant
    Filed: August 12, 2013
    Date of Patent: July 2, 2019
    Inventors: Brian Gillette, Samuel Sia
  • Patent number: 10336986
    Abstract: The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.
    Type: Grant
    Filed: February 12, 2018
    Date of Patent: July 2, 2019
    Assignee: National Central University
    Inventors: Akon Higuchi, Ta-Chun Kao