Abstract: Described herein are biological devices and methods for using the same to produce carbo sugars. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a cellulose synthase and galactomannan galactosyltransferase. In some instances, the biological devices also include a gene for lipase. Methods for altering the viscosity of petroleum oil using the carbo sugars are also described herein. Finally, methods for degreasing or decontaminating water mixed with petroleum oil or other fatty substances or a surface coated with petroleum oil or other fatty substances using the carbo sugars are described herein.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
October 19, 2021
Assignee:
BIOCAPITAL HOLDINGS, LLC
Inventors:
Raul Cuero Rengifo, Juliana Londoño Murillo, Mariana Sanchez Londoño, Daniel Agudelo Valencia, Juan Miguel Sanchez Bustamante, Mario Antonio Franco Jimenez, Lina Clemencia Sanchez Bustamante
Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
Abstract: The present disclosure describes a Mtu ?I-CM intein variant containing one or more mutations or a biologically active fragment thereof, and a method for producing and purifying a molecule of interest using the intein variant. Further described are isolated fusion proteins comprising the intein variant and a tag and a molecule of interest. Also described are expression systems for expressing the intein variant as well as polypeptide screening methods employing the intein variant.
Abstract: Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.
Type:
Grant
Filed:
February 5, 2020
Date of Patent:
October 5, 2021
Assignee:
LEVADURA BIOTECHNOLOGY, INC.
Inventors:
Alexander Hutagalung, Jose Miguel Laplaza
Abstract: Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.
Type:
Grant
Filed:
January 17, 2020
Date of Patent:
September 28, 2021
Assignee:
Danisco US INC.
Inventors:
Vivek Sharma, Paula Johanna Maria Teunissen
Abstract: The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome of E coli prompted by strong promoter Ptrc to reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Ptrc on the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.
Type:
Grant
Filed:
May 12, 2019
Date of Patent:
September 21, 2021
Assignee:
Tianjin University of Science and Technology
Abstract: The present invention relates to a novel method for purifying 3,6-anhydro-L-galactose by using microorganisms and provides an effect of improving the production yield of 3,6-anhydro-L-galactose by using microorganisms during purification after enzymatic hydrolysis of agarose or agar.
Type:
Grant
Filed:
March 31, 2017
Date of Patent:
September 14, 2021
Assignee:
Korea University Research and Business Foundation
Inventors:
Kyoung Heon Kim, In-Geol Choi, Sora Yu, Eun-Ju Yun, Kyung-mun Cho
Abstract: Provided is an engineered pathway that can function in a cell-free system, cellular system or a combination thereof to convert a sugar to a chemical or biofuel.
Type:
Grant
Filed:
July 21, 2016
Date of Patent:
August 31, 2021
Assignee:
The Regents of the University of California
Inventors:
James U. Bowie, Tyler P. Korman, Paul H. Opgenorth
Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a liquor. The present invention also relates to methods of using the compositions.
Type:
Grant
Filed:
April 25, 2019
Date of Patent:
August 10, 2021
Assignees:
Novozymes, Inc., Novozymes North America, Inc.
Inventors:
Jason Quinlan, Feng Xu, Matt Sweeney, Don Higgins, Hui Xu
Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
Type:
Grant
Filed:
December 22, 2016
Date of Patent:
August 3, 2021
Assignee:
DSM IP ASSETS B.V.
Inventors:
Alrik Pieter Los, Zheng Zhao, René Verwaal, Jacobus Thomas Pronk, Rolf Poldermans
Abstract: Disclosed herein are materials and methods for the production of high concentrations of ethanol from plant starch material broken down into disaccharide and trisaccharide sugars such as maltose, isomaltose, and maltotriose from uncooked starch. Herein is also described use of a yeast strain capable of fermenting high maltose syrups into ethanol without the need to convert disaccharides or trisaccharides into glucose using exogenous glucoamylases.
Type:
Grant
Filed:
December 28, 2018
Date of Patent:
August 3, 2021
Assignee:
Xylogenics, Inc.
Inventors:
Joshua W. Heyen, Mark G. Goebl, Kathryn A. Houin, Matthew S. Kelker, Nadaraj Palaniappan
Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.
Abstract: This invention relates to the biosynthesis of organic acids in genetically modified microorganisms. More specifically, this invention provides genetically modified microorganisms that are particularly tolerant to organic acids at low pH and are capable of producing organic acids by fermentation at low pH.
Type:
Grant
Filed:
September 13, 2013
Date of Patent:
July 27, 2021
Assignee:
PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITED
Inventors:
R. Rogers Yocum, Sudhanshu Dole, Janice G. Pero
Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.
Type:
Grant
Filed:
August 9, 2017
Date of Patent:
July 13, 2021
Assignee:
RIVER STONE BIOTECH, LLC
Inventors:
Esben Halkjaer Hansen, Markus Schwab, Philipp Berninger, Fanny Delgrange, Franziska Grassinger
Abstract: This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds.
Type:
Grant
Filed:
March 15, 2017
Date of Patent:
June 29, 2021
Assignee:
WILLIAM MARSH RICE UNIVERSITY
Inventors:
Ramon Gonzalez, James M. Clomburg, Seokjung Cheong
Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
Abstract: The present invention provides a mutant algal microorganism that has a mutation that causes attenuated expression of TrifuncB and/or TrifuncA and as a result produces more lipids than a control algal microorganism. The mutant algal microorganism can further include a mutation in a gene encoding a peroxisomal beta-oxidation pathway protein, such as an ACO1 or PXA1 gene, or a glyoxylate pathway protein, such as an ICL1 gene, that results in attenuated expression and further increased lipid production. Furthermore, provided herein are methods of producing lipids using the mutant algal microorganisms and methods of making the mutant microorganisms.
Abstract: The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided.
Type:
Grant
Filed:
August 28, 2019
Date of Patent:
June 22, 2021
Assignee:
Calysta, Inc.
Inventors:
Renee M. Saville, Joshua Silverman, Jeremy Minshull, Jon Edward Ness, Effendi Leonard, Jana Stumpe, Mark Welch