Abstract: In one embodiment, the invention provides a method of purifying recombinant alpha-galactosidase A. The method includes obtaining a lysate from cells recombinantly expressing alpha-galactosidase A grown in a cell culture medium having non-precipitating phosphate; contacting said lysate with a first chromatography media that binds ?-D-mannopyranosyl or ?-D-glucopyranosyl; eluting alpha-galactosidase A from the first chromatography media to generate a first eluate having alpha-galactosidase A, wherein said eluting includes at least one elution pause between 4 and 16 hours; contacting the first eluate with a second chromatography media that binds galactose binding proteins; and eluting alpha-galactosidase A from said second chromatography media to generate a second eluate containing said recombinant alpha-galactosidase A.
Type:
Grant
Filed:
March 5, 2018
Date of Patent:
December 1, 2020
Assignee:
Research Foundation of the City University of New York
Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.
Abstract: Disclosed are a method for preparing crystals of IsPETase protein, a method for screening an IsPETase protein activity regulator and IsPETase variants using a conformation of the protein crystal, a method for screening, IsPETase variants with increased PETase activity, and a method for decomposing PET using the variants. According to exemplary embodiments of the present invention, it is possible to determine a method for effectively preparing a crystal of the IsPETase protein and to obtain the resulting crystal thereof. Further, according to exemplary embodiments of the present invention, it is possible to identify a tertiary structure of the IsPETase from the crystal thereof and to prepare the variant with an increased PETase activity based on this structure. The IsPETase variant may be used effectively in the PET decomposition field.
Abstract: The present invention relates to a method for modifying one or more characteristics of a lipase, comprising the step of associating a peptide to the lipase, wherein the peptide has at least 50% sequence identity with the amino acid sequence of at least one major lipase contact zone of the propeptide of said lipase.
Type:
Grant
Filed:
February 5, 2019
Date of Patent:
December 1, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Anna Verena Reiser, Vikram Kjoeller Bhatia, Kim Borch, Christian I. Joergensen, Birgitte Andersen, Lone Baunsgaard
Abstract: Bleach-containing automatic dishwashing cleaning composition including a new amylase.
Type:
Grant
Filed:
April 9, 2019
Date of Patent:
November 24, 2020
Assignee:
The Procter & Gamble Company
Inventors:
Neil Joseph Lant, Philip Frank Souter, David John Tarbit, Torsten Bak Regueira, Bitten Plesner, Thomas Holberg Blicher, Anne Dorte Houg, Sofia Arnehed, Lars Lehmann Hylling Christensen, Carsten Andersen, Euan John Magennis
Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
Type:
Grant
Filed:
November 20, 2018
Date of Patent:
November 24, 2020
Assignee:
Codexis, Inc.
Inventors:
Jovana Nazor, Robert Osborne, Jack Liang, Jonathan Vroom, Xiyun Zhang, David Entwistle, Rama Voladri, Ravi David Garcia, Jeffrey C. Moore, Shane Grosser, Birgit Kosjek, Matthew Truppo
Abstract: The present invention relates to processes for recovering/extracting oil from fermentation product production processes based on starch-containing material, wherein an alpha-amylase, a high dosage of protease, and optionally a glucoamylase, are present and/or added in liquefaction. The invention also relates to processes for producing fermentation products and to enzyme compositions suitable for use in processes of the invention.
Type:
Grant
Filed:
September 4, 2018
Date of Patent:
November 24, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Suzanne Clark, John Matthews, Joseph Jump, Nathaniel Kreel
Abstract: Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include growing yeast on non-fermentative carbon sources. Other methods include growing yeast on one or more polysaccharides in which saccharification and fermentation of the polysaccharides occurs simultaneously.
Type:
Grant
Filed:
August 8, 2016
Date of Patent:
November 24, 2020
Assignee:
Cargill, Incorporated
Inventors:
James C. Anderson, Ting Liu Carlson, Arlene M. Fosmer
Abstract: Processes for grinding corn, ground corn products, and processes for making ethanol from the ground corn products. In some examples, a process for making ethanol can include introducing a plurality of corn pieces into a mill. The process can also include milling the corn pieces in the mill to produce a ground corn product. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 ?m, as measured according to AOAC 965.22-1966. Greater than 80 wt % of the ground corn product can have a particle size of 425 ?m or less, as measured according to AOAC 965.22-1966. The process can also include processing the ground corn product to produce a fermentation mash that can include ethanol and separating at least a portion of the ethanol from the fermentation mash to produce a stillage.
Abstract: The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of glucose into fructose wherein the glucose is derived from lignocellulosic material. More in particular, it provides a method for converting glucose into fructose comprising the steps of: providing a composition comprising water, glucose and lignin, enzymatically converting the glucose to fructose in the presence of a glucose isomerase, and optionally purifying the fructose from the solution, wherein the glucose isomerase comprises an amino acid sequence that is at least 90% identical with the sequence according to SEQ ID NO: 1 or SEQ ID NO: 2.
Type:
Grant
Filed:
June 20, 2017
Date of Patent:
November 17, 2020
Assignee:
METGEN OY
Inventors:
Klara Birikh, Anu Minna Maaret Suonpää, Matti Wilhelm Heikkilä
Abstract: A process for treating crop kernels is comprised of the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).
Type:
Grant
Filed:
November 25, 2016
Date of Patent:
November 17, 2020
Assignee:
Novozymes A/S
Inventors:
Yi Cao, James Lavigne, Bernardo Vidal, Jr., Thomas Patrick Gibbons, Chee-Leong Soong, Brian R. Scott, Randall Scott Deinhammer, Zhen Long, Michael John Akerman, Xinyu Shen, Yu Zhang
Abstract: The present disclosure discloses a production method of Danshensu, belonging to the technical field of bioengineering. The present disclosure constructs a novel genetic engineering strain co-expressed by three enzymes, which can be applied to the production of optically pure 3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid. All of the (D/L)-?-hydroxycarboxylic acid dehydrogenase selected by the present disclosure have the characteristics of poor substrate specificity and strong optical specificity, and can produce optically pure D-danshensu and L-danshensu. Further, the production efficiency of the recombinant strain is improved by knocking out or enhancing the expression of a related gene on the E. coli genome to promote substrate transport and reduce product decomposition.
Abstract: Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, ?-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The ?-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, Novosphingobium aromaticivorans, Escherichia coli, Streptococcus sanguinis, Phanerochaete chrysosporium, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.
Type:
Grant
Filed:
August 14, 2018
Date of Patent:
November 10, 2020
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventors:
Timothy James Donohue, Daniel Leo Gall, Wayne S. Kontur, Hoon Kim, John Ralph, Daniel R. Noguera, Brian Fox, Craig Bingman
Abstract: By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.
Type:
Grant
Filed:
May 21, 2019
Date of Patent:
November 3, 2020
Assignees:
ARKRAY, Inc., Ultizyme International Ltd.
Inventors:
Koji Sode, Kazushige Mori, Katsuhiro Kojima
Abstract: The present invention provides, inter alia, a method for combined preparation of saccharides, alcohols (biofuel) and biodiesel using a composition comprising interior hydrophobic or hydrophilic medium encapsulated by a layer comprising cellulose, cellulose derivative material, and/or starch surrounded by a hydrophilic or hydrophobic medium, respectively.
Type:
Grant
Filed:
September 7, 2016
Date of Patent:
October 27, 2020
Assignee:
TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED
Inventors:
Yachin Cohen, Dmitry M. Rein, Lev Vaikhanski, Gilad Alfassi
Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Type:
Grant
Filed:
January 10, 2018
Date of Patent:
October 13, 2020
Assignee:
Ginkgo Bioworks, Inc.
Inventors:
Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
Abstract: A method is provided for biosynthetic production of cannabinoids in microorganisms from a carbon source precursor. This method describes the genetic modifications needed to engineer microorganisms to produce cannabinoids as well as a method for identifying and quantifying cannabinoids from fermentation broth. A system is also provided for tuning the method to produce different cannabinoids of interest by systematically modulating the enzymes encoded by the genetic modifications introduced in the microorganism.
Type:
Grant
Filed:
June 14, 2019
Date of Patent:
October 13, 2020
Assignee:
SYNTIVA THERAPEUTICS, INC.
Inventors:
Adam Davidovich, Brian Chan, Neil Gupta, Alim Ladha
Abstract: The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of xylose into xylulose wherein the xylose is derived from lignocellulosic material.
Type:
Grant
Filed:
June 20, 2017
Date of Patent:
October 6, 2020
Assignee:
METGEN OY
Inventors:
Klara Birikh, Anu Minna Maaret Suonpää, Matti Wilhelm Heikkilä
Abstract: The present invention relates to cell lines that are genetically modified to overexpress a ?-galactoside ?-2,3-sialyltransferase 1 (ST3Gal1), preferably human ST3Gal1, which can be used for the production of recombinant glycoproteins having highly or fully sialylated O-linked GalNAc glycans (GalNAc O-glycans), preferably core 1 GalNAc O-glycans, as well as to respective recombinant glycoproteins. Further, the present invention relates to respective methods of expressing recombinant glycoproteins, methods of increasing the degree of sialylation of recombinant glycoproteins, and methods of decreasing the micro-heterogeneity of GalNAc O-glycans. Finally, the present invention relates to respective uses of the above cell lines for the production of recombinant glycoproteins, for increasing the degree of sialylation of recombinant glycoproteins, and for decreasing the micro-heterogeneity of O-linked GalNAc glycans of recombinant glycoproteins.