Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
Type:
Grant
Filed:
May 9, 2019
Date of Patent:
March 9, 2021
Assignee:
NOVOZYMES A/S
Inventors:
Randy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee Leong Soong, Tomoko Matsui, Shinobu Takagi
Abstract: The present invention provides a composition containing linalool, wherein the composition contains a high amount of either enantiomer R-linalool or S-linalool, and has a high content rate of linalool. The present invention also provides a production method for producing the composition. The present invention further provides a composition containing volatile components including linalool, in which a content of linalool in a total content of the volatile components in the composition is 60% or more, and the linalool is present as R-linalool or S-linalool in an amount of 50% or more of the enantiomer, and a production method therefor.
Abstract: Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.
Type:
Grant
Filed:
October 10, 2019
Date of Patent:
March 2, 2021
Assignee:
New England Biolabs, Inc.
Inventors:
Jennifer Ong, Nathan Tanner, Yinhua Zhang, Yanxia Bei, Vladimir Potapov
Abstract: Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.
Abstract: A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.
Type:
Grant
Filed:
July 3, 2018
Date of Patent:
February 23, 2021
Assignees:
ARKRAY, Inc., Ultizyme International Ltd.
Inventors:
Koji Sode, Junko Shimazaki, Kazushige Mori, Katsuhiro Kojima
Abstract: The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.
Abstract: In some embodiments, the present invention provides a recombinant protein comprising an affinity tag configured to attach the recombinant protein to a silicate surface, fused to a hydrogen sulfide scavenging enzyme.
Type:
Grant
Filed:
November 27, 2019
Date of Patent:
February 9, 2021
Assignee:
BAKER HUGHES, A GE COMPANY, LLC
Inventors:
Charles D. Armstrong, Frances H. Debenedictis
Abstract: In some embodiments, the present invention provides a recombinant protein comprising an affinity tag configured to attach the recombinant protein to a silicate surface, fused to a hydrogen sulfide scavenging enzyme.
Type:
Grant
Filed:
November 27, 2019
Date of Patent:
February 9, 2021
Assignee:
BAKER HUGHES, A GE COMPANY, LLC
Inventors:
Charles D. Armstrong, Frances H. Debenedictis
Abstract: The present invention provides for a method of producing a polysaccharide gel comprising: (a) culturing or growing a cyanobacterium or a mixture of cyanobacteria species in a medium under conditions such that the cyanobacterium or the mixture of cyanobacteria species produces a polysaccharide or a mixture of polysaccharides, (b) optionally separating the polysaccharide or the mixture of polysaccharides from the medium and/or the cyanobacterium or the mixture of cyanobacteria species, and (c) increasing the pH of the polysaccharide or the mixture of polysaccharides such that the polysaccharide or the mixture of polysaccharides forms a gel.
Type:
Grant
Filed:
April 2, 2018
Date of Patent:
February 2, 2021
Assignees:
The Regents of the University of California, HelioBioSys, Inc.
Inventors:
Mona Mirsiaghi, Eric Sundstrom, Deepti Tanjore, Todd Pray, Rocco L. Mancinelli, David T. Smernoff
Abstract: The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.
Abstract: Described herein are phospholipid nanogels that can contain a low concentration (less than about 250 ?Units/?L) of an exoglycosidase enzyme. Also described herein are systems and devices that can contain a phospholipid nanogel that can contain a low concentration (less than about 250 ?Units/?L) of an exoglycosidase enzyme. Also described herein are methods of using the phospholipid nanogels described herein and devices and systems that can contain a phospholipid nanogel described herein.
Abstract: Methods and compositions for the oxidation of short alkanes by engineered microorganisms expressing recombinant enzymes is described, along with methods of use.
Type:
Grant
Filed:
November 17, 2016
Date of Patent:
January 19, 2021
Assignee:
Industrial Microbes, Inc.
Inventors:
Elizabeth Jane Clarke, Baolong Zhu, Derek Lorin Greenfield, Stephanie Rhianon Jones, Noah Charles Helman
Abstract: The present invention relates to a novel ?-1,6-endoglucanase producing gentiobiose or glucose from ?-glucan, and more specifically, the present invention provides an effect of producing gentiobiose or glucose at high yield through ?-1,6-endoglucanase showing ?-1,6-endoglucanase activity on ?-glucan.
Type:
Grant
Filed:
June 30, 2017
Date of Patent:
January 5, 2021
Assignee:
Korea University Research and Business Foundation
Inventors:
Kyoung Heon Kim, Damao Wang, Do Hyoung Kim
Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
Type:
Grant
Filed:
November 13, 2017
Date of Patent:
January 5, 2021
Assignees:
Toyota Motor Corporation, Regents of the University of Minnesota
Abstract: The present application discloses a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/or electrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity. Contrary to the purification currently used in fermentative production of neutral HMOs, the presented process allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filtered concentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. pet food) applications.
Abstract: Provided is a method of producing 2?-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express ?-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
Type:
Grant
Filed:
October 1, 2019
Date of Patent:
December 29, 2020
Assignees:
SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, ADVANCED PROTEIN TECHNOLOGIES CORP.
Inventors:
Jin-Ho Seo, Young-Wook Chin, Hae-Yong Jo
Abstract: The present invention describes an efficient method for producing linalool. The present invention provides a method for producing linalool, the method including culturing a microorganism expressing linalool synthase in a culture medium to produce linalool. The present invention also describes a microorganism able to express linalool synthase and efficiently produce linalool.
Abstract: Provided is a gene encoding alanyl-glutamine dipeptide biosynthetic enzyme and the application thereof. The nucleotide sequence of said gene is shown in SEQ ID NO: 1. Further, provided is an amino acid sequence encoded by the gene, and provided recombinant vector, recombinant E. coli, recombinant S. cerevisiae, and a self-flocculating recombinant S. cerevisiae containing the gene. Furthermore, provided is a method for carrying out bioconversion to synthesize alanyl-glutamine dipeptide by using said recombinant microorganisms. The application of the gene and recombinant batteries has the characteristics of high molar conversion rate, fast production rate and repeated recycling of the bacteria. It is a good choice for efficient, environmentally friendly and economical production of alanyl-glutamine dipeptide. In addition, through the recycling process, it is expected to reduce the cost of industrial production, solve the problems of difficult enzyme recovery, low utilization rate and unstable enzyme activity.
Abstract: The present disclosure discloses a maltooligosyl trehalose synthase mutant with improved thermal stability, and belongs to the technical fields of enzyme engineering and protein engineering. The residual enzyme activities of the MTSase mutants S361R, S444E, S361R/S444E, S361K/S444E, G415P/S361R/S444E and G415P consistent with the present disclosure after treatment at 60° C. for 10 min are respectively 70.3%, 50.1%, 83.5%, 65.9%, 100% and 80.7%, which are respectively 1.6, 1.1, 1.9, 1.5, 2.3 and 1.9 times of that of the wild type. The half-lives of the S361R/S444E and G415P/S361R/S444E at 60° C. are respectively 14.9 min and 90.8 min which are respectively 3.2 and 19.7 times of that of the wild type, indicating that the thermal stability of the MTSase mutant consistent with the present disclosure is significantly improved than that of the wild type.
Type:
Grant
Filed:
May 30, 2019
Date of Patent:
December 15, 2020
Assignee:
JIANGNAN UNIVERSITY
Inventors:
Jing Wu, Lingqia Su, Chun Chen, Zirui Wang, Jinyun Feng
Abstract: Disclosed is a continuous process for separating or extracting proteins from a low grade mixture of a protein of interest, other proteins, impurities, and salts in a continuous simulated moving bed separation process. The invention provides for direct extraction of heme protein and plant protein from a crude mixture of such proteins, other proteins, impurities and salts using the chromatographic technique of simulated moving bed (SMB) continuous chromatography. The SMB process combines the steps of feed loading, adsorbent washing, product elution, adsorbent regeneration, and adsorbent equilibration. The novel strong anion exchange resin adsorbent is a quaternary amine cross-linked microcellulose wherein the microcellulose is cross-linked with epichlorohydrin and the quaternary amine is 2,3-epoxypropyltrimethyl-ammonium chloride which exhibits selective adsorption of proteins and complete regeneration.
Type:
Grant
Filed:
November 20, 2017
Date of Patent:
December 8, 2020
Assignee:
Orochem Technologies Inc.
Inventors:
Anil R Oroskar, Babu Siddegowda Antharavally, Anantha Krishna Mallia