Patents Examined by Christian L. Fronda
  • Methods and systems for 1-butanol production
    Patent number: 10696988
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 30, 2020
    Assignee: Biocheminsights, Inc.
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas
  • Method for the fermentative production of L-lysine by modified
    Patent number: 10689677
    Abstract: A method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum, having the ability to excrete L-lysine and containing in their chromosome a mutated NCgl2816 polynucleotide. Further, the method is used for cultivating the bacteria in a suitable medium under suitable conditions, and accumulating said L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: June 23, 2020
    Assignee: Evonik Operations GmbH
    Inventors: Frank Schneider, Georg Thierbach, Thomas Bekel, Kornelia Voβ
  • Methods for treating or preventing HBV infection or HBV related diseases
    Patent number: 10662414
    Abstract: The present invention relates to polymerase HBV mutant polypeptides comprising a mutated polymerase domain which is functionally disrupted for polymerase activity and fusion proteins comprising such polymerase mutant polypeptide. The present invention also relates to a nucleic acid molecule and an expression vector for expressing said polymerase mutant polypeptide as well as a composition which can be used for eliciting an immune response to HBV with the goal of providing a protective or therapeutic effect against HBV infection.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: May 26, 2020
    Assignee: Transgene S.A.
    Inventors: Perrine Martin, Nathalie Silvestre, Jean-Baptiste Marchand
  • Strain of meyerozyma guilliermondii and its use in methods of catalytic synthesis of 2,5-dihydroxymethylfuran
    Patent number: 10662452
    Abstract: A yeast strain and a method for the synthesis of 2,5-dihydroxymethylfuran using this strain are disclosed. The yeast strain is Meyerozyma guilliermondii SC 1103, which has been maintained in the China Center for Type Culture Collection (CCTCC, Wuhan, P.R. China) with an access No. of M2016144. The method for the synthesis of 2,5-dihydroxymethylfuran using this strain is described as follows: after pre-cultivation and cultivation, Meyerozyma guilliermondii SC 1103 cells are added into the buffer solutions containing 5-hydroxymethylfurfural and glucose; the biocatalytic reaction is conducted under designated conditions, thus affording 2,5-dihydroxymethylfuran. This disclosure has many advantages such as good selectivity, mild reaction conditions, environmental friendliness, high efficiency, and good yield.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: May 26, 2020
    Assignee: SOUTH CHINA UNIVERSITY OF TECHNOLOGY
    Inventors: Ning Li, Yanmei Li, Minhua Zong
  • Artificial nucleases including engineered FokI cleavage half-domains
    Patent number: 10640790
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Grant
    Filed: August 6, 2018
    Date of Patent: May 5, 2020
    Assignee: Sangamo Therapeutics, Inc.
    Inventors: Yannick Doyon, Jeffrey C. Miller
  • Polysaccharide compositions for absorbing aqueous liquid
    Patent number: 10639611
    Abstract: Compositions are disclosed herein comprising poly alpha-1,3-glucan or poly alpha-1,3-1,6-glucan polymer having an open-cell pore structure with an average pore size of about 100 nm to about 3000 nm in diameter. Such polymers have enhanced aqueous liquid absorption capacity, and can be comprised in various products having aqueous liquid absorption function. Methods of preparing glucan polymers of the compositions provided herein are also disclosed.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: May 5, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Natnael Behabtu, Rakesh Nambiar
  • Processes of producing fermentation products
    Patent number: 10640794
    Abstract: The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: May 5, 2020
    Assignee: NOVOZYMES NORTH AMERICA, INC.
    Inventors: Chee-Leong Soong, Jiyin Liu, Guillermo Coward Kelly
  • Yeast strains with reduced fatty alcohol oxidase activity and method for the production of omega-hydroxy fatty acids and dicarboxylic acids
    Patent number: 10640796
    Abstract: The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ?-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ?-hydroxy fatty acids or dicarboxylic acids is advantageously increased.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: May 5, 2020
    Assignee: Provivi, Inc.
    Inventors: Michael Gatter, Falk Matthaus, Gerold Barth
  • Method for preparing mogroside having no ?-1,6-glucoside bond
    Patent number: 10626430
    Abstract: The present invention provides a method for preparing a mogroside having no ?-1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one ?-1,6-glucoside bond, thereby cleaving said ?-1,6-glucoside bond.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: April 21, 2020
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Eiichiro Ono
  • Chloroalkane penetration method for quantifying access of a molecule into a cell
    Patent number: 10620214
    Abstract: As described below, the invention provides methods for localizing and quantifying the extent to which a molecule penetrates a cell.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: April 14, 2020
    Assignee: TRUSTEES OF TUFTS COLLEGE
    Inventors: Joshua Kritzer, Leila Peraro
  • Method of producing saccharides from biomass with lesser amount of saccharifying enzyme inexpensively
    Patent number: 10612062
    Abstract: A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: April 7, 2020
    Assignee: JGC Corporation
    Inventor: Daisuke Taneda
  • Methods for saccharifying a starch substrate
    Patent number: 10612058
    Abstract: Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: April 7, 2020
    Assignee: DANISCO US INC
    Inventors: Vivek Sharma, Paula Johanna Maria Teunissen
  • BRCA2 mediated protein purification recombinase
    Patent number: 10604778
    Abstract: The invention is the products and methods associated with purifying overexpressed recombinant recombinases from a host cell line resulting in an un-tagged protein of interest without any additional, non-native amino acids. The invention employs at least one DNA vector that co-expresses a tagged fusion protein and the recombinase protein with the recombinase protein having an affinity for binding to the the tagged fusion protein. Isolation methods of the recombinase protein include the targeting of the tagged fusion protein.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: March 31, 2020
    Assignee: IDEA SEED, LLC
    Inventor: Michael Longo
  • Polypeptide having the activity of exporting O-acetyl-homoserine
    Patent number: 10597686
    Abstract: The present disclosure relates to a protein having the activity of exporting O-acetylhomoserine and a novel modified protein thereof, a microorganism capable of producing O-acetylhomoserine with enhanced expression of the protein, and a method for producing O-acetylhomoserine using the microorganism.
    Type: Grant
    Filed: July 17, 2019
    Date of Patent: March 24, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Jee Yeon Bae, Ji Hyun Shim, Hyun Ah Kim, Juhee Seo, Yong Uk Shin, Jae Hee Lee, Sang Kyoum Kim, So Young Kim
  • Compositions for cell culture and methods of using the same
    Patent number: 10590457
    Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: March 17, 2020
    Assignee: BRISTOL MYERS-SQUIBB COMPANY
    Inventors: Jun Tian, Jianlin Xu, Qin He
  • Methods for reducing content of hexenuronic acids in cellulosic pulp
    Patent number: 10570562
    Abstract: The present invention provides an enzymatic method for reducing the content of hexenuronic acids in a chemical cellulosic pulp and/or improvement of the brightness of cellulosic pulp using haloperoxidase.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: February 25, 2020
    Assignee: Novozymes A/S
    Inventors: Henrik Lund, Klaus Skaalum Lassen, Bjoern Lennart Pierre Alexander Cassland, Pedro Emanuel Garcia Loureiro
  • Corynebacterium glutamicum for use in producing 2?-fucosyllactose
    Patent number: 10570399
    Abstract: Disclosed are a recombinant Corynebacterium glutamicum (C. glutamicum) for producing fucosyllactose which is transformed to express ?-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosyllactose using the same. According to the recombinant Corynebacterium glutamicum and the method for producing fucosyllactose according to the present invention, with use of a GRAS Corynebacterium glutamicum strain, which is safer than conventional Escherichia coli, 2?-fucosyllactose can be produced at a high concentration while overcoming drawbacks of conventional methods associated with industrial inapplicability resulting from low production concentrations.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: February 25, 2020
    Assignee: Seoul National University R&DB Foundation
    Inventors: Jin-Ho Seo, Young-Wook Chin, Hae-Yong Jo
  • Process of using a plug flow hydrolysis reactor having a slurry introduction device
    Patent number: 10563239
    Abstract: Provided herein is a process for hydrolyzing a cellulosic feedstock to produce sugar. The process comprises introducing a pretreated cellulosic slurry to an inlet region of a plug flow hydrolysis reactor using a slurry introduction device that reduces the axial momentum of the slurry at the surface of the reactor contents. The cellulosic feedstock slurry is hydrolyzed in the plug flow hydrolysis reactor by contacting the cellulosic feedstock with at least cellulase enzymes to produce glucose. Also provided herein is a vertically-oriented, unmixed downflow hydrolysis reactor for hydrolyzing a pretreated cellulosic feedstock slurry which comprises such a slurry introduction device disposed in a top region thereof.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: February 18, 2020
    Assignee: Iogen Energy Corporation
    Inventors: Torbjorn van der Meulen, Lee Castiglione, Stephanie Lepine
  • Recombinant microorganisms and methods for producing cannabinoids and cannabinoid derivatives
    Patent number: 10563211
    Abstract: The present disclosure provides genetically modified host cells that produce a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative. The present disclosure provides methods of synthesizing a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: February 18, 2020
    Assignee: The Regents of the University of California
    Inventors: Jay D. Keasling, Leo D'Espaux, Jeff Wong, Xiaozhou Luo, Michael Reiter, Charles Denby, Anna Lechner
  • Compositions for mitigating hydrogen sulfide contamination using a recombinant protein with an affinity tag fused to a hydrogen sulfide scavenging enzyme
    Patent number: 10556815
    Abstract: In some embodiments, the present invention provides a recombinant protein comprising an affinity tag configured to attach the recombinant protein to a silicate surface, fused to a hydrogen sulfide scavenging enzyme.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 11, 2020
    Assignee: BAKER HUGHES, A GE COMPANY, LLC
    Inventors: Charles D. Armstrong, Frances H. Debenedictis