Patents Examined by David Steadman
  • Recombinant yeast for producing 2,3-butanediol including pyruvate decarboxylase derived from candida tropicolis and method for producing 2,3-butanediol using the same
    Patent number: 10982236
    Abstract: Provided is a recombinant Saccharomyces cerevisiae for producing 2,3-butanediol, wherein all GPD1 and GPD2 genes involved in glycerol biosynthesis are removed and a gene encoding NADH oxidase is introduced, and wherein pyruvate decarboxylase activity is inactivated and Candida tropicalis PDC1 gene encoding Candida tropicalis pyruvate decarboxylase 1-is introduced, and wherein expression of the Candida tropicalis PDC1 gene is regulated by a GPD2 promoter.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: April 20, 2021
    Assignee: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION
    Inventors: Jin Ho Seo, Jin Woo Kim
  • Modified RNA polymerase sigma factor 70 polypeptide
    Patent number: 10968467
    Abstract: The present invention relates to a novel variant RNA polymerase sigma factor 70 (?70) polypeptide, a polynucleotide encoding the same, a microorganism containing the polypeptide, and a method for producing L-threonine by using the microorganism.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: April 6, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ji Sun Lee, Kwang Ho Lee, Hyo Jin Kim, Keun Chul Lee, Young Bin Hwang
  • Diphosphomevalonate decarboxylase variant and method for producing olefin compound by using the same
    Patent number: 10961548
    Abstract: Mutations involving amino acid substitution were introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Then, the variants were each evaluated in terms of a catalytic activity for production of olefin compounds such as isoprene. As a result, it was found that substitution of glycine at position with a different amino acid resulted in improvement in the catalytic activity. In addition, it was found that the MVD in which arginine at position and threonine at position in addition to the position were further substituted with different amino acids, respectively, also had the high catalytic activity.
    Type: Grant
    Filed: September 26, 2017
    Date of Patent: March 30, 2021
    Assignees: RIKEN, ZEON CORPORATION, THE YOKOHAMA RUBBER CO., LTD.
    Inventors: Ryoko Orishimo, Tomokazu Shirai, Kazuhiro Takahashi, Misao Hiza, Yusuke Tanabe
  • Recombinant microorganism having enhanced 2,3-butanediol producing ability and method for producing 2,3-butanediol using the same
    Patent number: 10947547
    Abstract: Disclosed herein is a recombinant microorganism having enhanced 2,3-butanediol producing ability, wherein a pathway for converting pyruvate to acetyl-CoA, a pathway for converting pyruvate to formic acid, or a pathway for converting pyruvate to lactate is inhibited in a microorganism having acetyl-CoA and lactate biosynthetic pathways.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: March 16, 2021
    Assignee: GS CALTEX CORPORATION
    Inventors: Jong-Myoung Park, Hyo-Hak Song, Taek-Ho Yang
  • Production of organic acids from cis-aconitic acid decarboxylase (cadA) deletion strains
    Patent number: 10947548
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: March 16, 2021
    Assignee: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Methods for production of oxygenated terpenes
    Patent number: 10934564
    Abstract: The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: March 2, 2021
    Assignee: MANUS BIO INC.
    Inventors: Ajikumar Parayil Kumaran, Chin Giaw Lim, Liwei Li, Souvik Ghosh, Christopher Pirie, Anthony Qualley
  • Gene targets for improved enzyme production in fungi
    Patent number: 10934551
    Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: March 2, 2021
    Assignees: Battelle Memorial Institute, Natl Tech & Engineering Solutions of Sandia, LLC
    Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
  • D-type amino acid dehydrogenase
    Patent number: 10927354
    Abstract: An enzyme has an activity of reversible dehydrogenation of D-amino acid and is a hexamer of polypeptides having an amino acid sequence that has 80% or more identity with the amino acid sequence of SEQ ID NO: 2. The amino acid sequence of the polypeptide can include one or more amino acid substitutions for one or more amino acid residues of SEQ ID NO: 2.
    Type: Grant
    Filed: August 9, 2018
    Date of Patent: February 23, 2021
    Assignees: KUREHA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Hironaga Akita, Yuusuke Nakamichi, Masahiro Watanabe, Akinori Matsushika, Tomotake Morita
  • Acidic thermophilic polygalacturonase TEPG28A, and encoding gene and application thereof
    Patent number: 10920206
    Abstract: Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: February 16, 2021
    Assignee: FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF Agricultural Sciences
    Inventors: Bin Yao, Huiying Luo, Yeqing Li, Yuan Wang, Tao Tu, Huoqing Huang, Yingguo Bai, Yaru Wang, Xiaoyun Su
  • Glucose oxidase CnGODA and gene and application thereof
    Patent number: 10920200
    Abstract: Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.
    Type: Grant
    Filed: June 7, 2018
    Date of Patent: February 16, 2021
    Assignee: FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
    Inventors: Bin Yao, Huiying Luo, Weina Liu, Jianzhong Ge, Tao Tu, Huoqing Huang, Xiaoyun Su, Yingguo Bai, Yuan Wang, Yaru Wang, Kun Meng
  • Methods of recombinantly producing neutral protease originating from
    Patent number: 10889809
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: January 12, 2021
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Polypeptide and method for producing ornithine-based product using the same
    Patent number: 10870871
    Abstract: The present disclosure relates to a novel polypeptide having an ability to export an ornithine-based product, and a method for producing an ornithine-based product using the same.
    Type: Grant
    Filed: June 14, 2018
    Date of Patent: December 22, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Seon Hye Kim, Su Jin Park, Kyoung Min Lee, Kyungsu Na, Hong Xian Li, Hyun-jung Bae, Jihyun Shim, Young Lyeol Yang, Hye Won Um, Hyo Hyoung Lee, Min Gyeong Kang, Hye Won Kim, Byeong Cheol Song, Haena Oh, Han Hyoung Lee
  • Lipase variants and polynucleotides encoding same
    Patent number: 10865366
    Abstract: The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: December 15, 2020
    Assignee: Novozymes A/S
    Inventors: Carsten Hoerslev Hansen, Jesper Vind, Luise Erlandsen, Bitten Plesner, Anna Catharina Olsen Stenholm
  • Engineered glucosyltransferases
    Patent number: 10865393
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: December 15, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Yougen Li, Ellen D. Semke, Qiong Cheng, Jian Ping Lai
  • Recombinant host cell engineered to overexpress helper proteins
    Patent number: 10865416
    Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: December 15, 2020
    Assignees: Boehringer Ingelheim RCV GmbH & Co KG, Vaitdogen GmbH, Lonza Ltd.
    Inventors: Brigitte Gasser, Diethard Mattanovich, Markus Buchetics
  • Polypeptides with lipase activity and polynucleotides encoding same
    Patent number: 10858637
    Abstract: The present invention relates to isolated polypeptides with lipase activity. The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the polynucleotides and methods of using the polypeptides or the compositions.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: December 8, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Kenneth Jensen, Maria Louise Leth, Anna Verena Reiser, Robert Piotr Olinski, Preben Nielsen, Lone Baunsgaard
  • Polypeptides
    Patent number: 10844360
    Abstract: The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: November 24, 2020
    Assignee: Novozymes A/S
    Inventors: Morten Gjermansen, Klaus Gori, Henrik Marcus Geertz-Hansen, Jesper Salomon, Mary Ann Stringer, Thomas Holberg Blicher, Nikolaj Spodsberg
  • Polypeptides having xylanase activity and polynucleotides encoding same
    Patent number: 10842172
    Abstract: The present invention relates to polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides of the invention to release xylose and in animal feed.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: November 24, 2020
    Assignee: Novozymes A/S
    Inventors: Charlotte Blom, Ninfa Rangel Pedersen, Dan Pettersson, Jens Magnus Ekloef, Kristian Bertel Roemer Krogh, Martin Simon Borchert, Dorotea Raventos Segura, Nele Ilmberger, Wolfgang Streit, Jesper Salomon
  • Calcium reporter gene
    Patent number: 10836802
    Abstract: As a calcium indicator protein having an excellent fluorescent characteristic and calcium reactivity, there is provided DNA in which one of a nucleotide sequence derivative of a calmodulin-binding sequence (ckkap sequence) of calcium/calmodulin-dependent protein kinase kinase and a nucleotide sequence encoding a calcium-binding sequence (CaM sequence) of calmodulin is linked to a 5? end of a nucleotide sequence encoding a fluorescent protein, and the other nucleotide sequence is linked to a 3? end of the nucleotide sequence encoding the fluorescent protein. The calcium indicator protein encoded by this DNA, which based on the derivative of the ckkap sequence as a binding domain for the calcium-bound CaM sequence, exhibits a fluorescent characteristic and calcium reactivity superior to those of conventional calcium indicator proteins.
    Type: Grant
    Filed: June 8, 2015
    Date of Patent: November 17, 2020
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Haruhiko Bito, Masatoshi Inoue, Atsuya Takeuchi, Junichi Nakai, Masamichi Ohkura
  • Expression cassette and host cell for expressing a Vip3-interacting protein
    Patent number: 10836801
    Abstract: This invention provides polypeptides that were identified as interacting with Vip3 toxin. This invention also provides a method of identifying agents that bind to Vip3 interacting polypeptides, which agents may act as insecticidal agent, cytotoxic agents and/or modulate the activity of Vip3 interacting polypeptides.
    Type: Grant
    Filed: August 10, 2018
    Date of Patent: November 17, 2020
    Assignee: Syngenta Crop Protection AG
    Inventors: Milan Jucovic, Renshui Liu, Richard Sessler, Guo-Qing Tang, Jeng Shong Chen