Abstract: The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.
Type:
Grant
Filed:
September 13, 2019
Date of Patent:
November 10, 2020
Assignee:
ROQUETTE FRERES
Inventors:
Astrid Schaefer, Melanie Diefenbacher, Yiming Chang, Sumire Honda Malca, Markus Schwab, Sophie Defretin, Tania Gerard, Arnaud Heysen, Friederike Thor
Abstract: A bacterium of the species Corynebacterium glutamicum has the ability to excrete L-lysine, and contains in its chromosome a polynucleotide encoding a mutated polypeptide having the assumed function of an acyltransferase, hydrolase, alpha/beta hydrolase or of a pimeloyl-ACP methyl ester esterase. Also, a method is used for producing L-lysine using such bacterium.
Type:
Grant
Filed:
December 18, 2019
Date of Patent:
November 10, 2020
Assignee:
Evonik Operations GmbH
Inventors:
Frank Schneider, Georg Thierbach, Kornelia Voß, Thomas Bekel
Abstract: The present invention relates to a genetically modified microorganism for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, and to a fermentation process for performing said conversion.
Type:
Grant
Filed:
October 23, 2015
Date of Patent:
October 13, 2020
Assignee:
METABOLIC EXPLORER
Inventors:
Gwenaëlle Corre, Laurence Dumon-Seignovert, Philippe Soucaille
Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Type:
Grant
Filed:
June 17, 2019
Date of Patent:
October 6, 2020
Assignee:
Codexis, Inc.
Inventors:
Oscar Alvizo, Melissa Ann Mayo, Jeffrey C. Moore
Abstract: The invention relates to provision of artificial bioluminescent enzymes. The invention provides for a polypeptide, including any one of amino acid sequences (i) to (iii) below, and having luciferase activity: (i) an amino acid sequence of SEQ ID NO: 1; (ii) an amino acid sequence of SEQ ID NO: 1 in which 1 to 10 amino acids are substituted, added, or deleted; or (iii) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.
Type:
Grant
Filed:
September 30, 2016
Date of Patent:
September 29, 2020
Assignee:
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.
Type:
Grant
Filed:
June 18, 2018
Date of Patent:
September 15, 2020
Assignee:
BRASKEM S.A.
Inventors:
Avram Michael Slovic, Iuri Estrada Gouvea, Daniel Johannes Koch, Felipe Galzerani
Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Type:
Grant
Filed:
April 9, 2018
Date of Patent:
September 15, 2020
Assignee:
Promega Corporation
Inventors:
Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
Abstract: The present invention relates to a novel variant RNA polymerase sigma factor 70 (?70) polypeptide, a polynucleotide encoding the same, a microorganism containing the polypeptide, and a method for producing L-threonine by using the microorganism.
Type:
Grant
Filed:
September 4, 2015
Date of Patent:
September 1, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Ji Sun Lee, Kwang Ho Lee, Hyo Jin Kim, Keun Chul Lee, Young Bin Hwang
Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of hydroxycinnamic acids using polypeptides having tyrosine ammonia lyase activity. More particularly, the present invention pertains to polypeptides having tyrosine ammonia lyase activity and high substrate specificity towards tyrosine, which makes them particularly suitable in the production of p-coumaric acid and other hydroxycinnamic acids. The present invention thus provides processes for the production of p-coumaric acid and other hydroxycinnamic acids employing these polypeptides as well as recombinant host cells expressing same.
Type:
Grant
Filed:
July 14, 2015
Date of Patent:
August 25, 2020
Assignee:
CYSBIO APS
Inventors:
Christian Bille Jendresen, Solvej Siedler, Steen Gustav Stahlhut, Alex Toftgaard Nielsen
Abstract: Disclosed herein are enzymes and organisms useful for the dealkylation of products derived from lignin depolymerization, including the conversion of guaiacol or guaethol to catechol or the conversion of anisole to phenol. Methods of converting guaiacol or guaethol to catechol or anisole to phenol using enzymes or organisms expressing the same are also disclosed.
Type:
Grant
Filed:
December 6, 2018
Date of Patent:
August 18, 2020
Assignee:
Alliance for Sustainable Energy, LLC
Inventors:
Christopher W. Johnson, Gregg T. Beckham
Abstract: The present invention relates to the expression and optimization of enzymes involved in the breakdown of lignocellulosic biomass. Disclosed are variants of the exoglucanase 1 of Trichoderma reesei, as well as the use of said variants with improved efficiency in methods for breaking down cellulose and for producing biofuel.
Type:
Grant
Filed:
June 12, 2015
Date of Patent:
August 18, 2020
Assignees:
IFP Energies Nouvelles, Centre National De La Recherche Scientifique—CNRS, Proteus
Abstract: The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.
Type:
Grant
Filed:
December 10, 2018
Date of Patent:
August 4, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Johan Börjesson, Anders Viksoe-Nielsen, Nikolaj Spodsberg, Kristian Krogh
Abstract: In some embodiments, the present invention provides a protein comprising amino acids in the following sequence L(X)n=14X3(X)n=1X1(X)n=1E(X)n=4P(X)n=1NR(X)n=3S(X)n=4D(X)n=2G(X)n=7Y(X)n=4Y (X)n=32-34X2 (SEQ ID NO: 128), wherein each X independently represents any naturally occurring amino acid residue and n indicates the number of amino acid residues represented by the respective paranthetical at that position, wherein: a) X1 is selected from the group consisting of S, C, T, M, V, Y, N, P, L, G, Q, A, I, D, W, H, or E, wherein if X1 is L, H or V, then X3 is D; and/or b) X2 is selected from the group consisting of H, L, S or V. In some embodiments, the present invention also provides a protein comprising amino acids in the sequence set forth by SEQ ID NO: 38 or SEQ ID NO: 39, except that: the amino acid at position 406 is an amino acid other than F; and/or the amino acid at position 474 is an amino acid other than N.
Type:
Grant
Filed:
December 4, 2015
Date of Patent:
June 23, 2020
Assignee:
SmartZyme Innovations Ltd.
Inventors:
Chen Haim Guttman, David Baram, Dotan Omer
Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
Type:
Grant
Filed:
September 14, 2018
Date of Patent:
June 23, 2020
Assignee:
Codexis, Inc.
Inventors:
Robert Osborne, Vesna Mitchell, Khin Yu Naing Htwe, Xiyun Zhang, Erika M. Milczek, Jeffrey C. Moore
Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and optionally on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
Type:
Grant
Filed:
June 27, 2014
Date of Patent:
May 12, 2020
Assignee:
Novozymes A/S
Inventors:
Jens Erik Nielsen, Pernille Ollendorff Hede, Jurgen Carsten Franz Knotzel, Maria Norman Hockauf, Lars Beier, Mikael Bauer, Annette Helle Johansen, Lars Lehmann Hylling Christensen, Julie Bille Rannes
Abstract: The present invention relates to a microorganism of the genus Corynebacterium having an ability to produce L-arginine, and a method of producing L-arginine using the same.
Type:
Grant
Filed:
October 13, 2015
Date of Patent:
April 21, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Hyun Ae Bae, Han Hyoung Lee, Min Gyeong Kang, Jong Hyun Kim, Hye Won Kim
Abstract: The present invention relates to a recombinant cyanobacterium with enhanced halotolerance and compositions thereof, methods of producing the recombinant cyanobacterium, and methods of using the same for biofuel production. The invention also relates to transformed F. diplosiphon strains with enhanced salt tolerance.
Abstract: The invention relates to the enzymatic preparation of galacto-oligosaccharides (GOS). Provided is a method for preparing GOS from lactose, comprising (i) contacting a lactose feed with immobilized beta-galactosidase (EC 3.2.1.23) and (ii) allowing for GOS synthesis, wherein said lactose feed is an aqueous slurry of crystalline lactose.
Type:
Grant
Filed:
September 4, 2014
Date of Patent:
March 17, 2020
Assignee:
Friesland Campina Nederland B.V.
Inventors:
Frédéric Benjamins, Linqiu Cao, Antonius Augustinus Broekhuis
Abstract: The present invention relates to a method for producing highly-concentrated L-amino acid and riboflavin simultaneously, and a microorganism for simultaneously producing L-amino acid and riboflavin. Specifically, the present invention relates to a modified microorganism for producing L-lysine or L-threonine, and riboflavin simultaneously, wherein the microorganism belonging to Corynebacterium sp. capable of producing L-lysine or L-threonine is modified by enhancing the activity of an enzyme family expressed by a rib operon which contains riboflavin biosynthesis gene family. Also, the present invention relates to a method for the simultaneous production of L-lysine or L-threonine, and riboflavin using the modified microorganism, and relates to a formulation or granular formulation, feed, and feed additive, containing L-lysine or L-threonine, and riboflavin produced from a culture medium of the modified microorganism.
Type:
Grant
Filed:
October 17, 2012
Date of Patent:
March 17, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Sang Hee Park, Jun Ok Moon, Sang Jo Lim, Do Hyun Kwon, Kyung Han Lee, Jin Suck Sung, Hyun Joon Kim