Patents Examined by David Steadman
  • Glucoamylase variants and polynucleotides encoding same
    Patent number: 10584324
    Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: March 10, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
  • Cellobiohydrolase variants and polynucleotides encoding same
    Patent number: 10584325
    Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: March 10, 2020
    Assignee: Novozymes A/S
    Inventors: Kim Borch, Kenneth Jensen, Kristian Krogh, Brett McBrayer, Peter Westh, Jeppe Kari, Johan Olsen, Trine Sorensen, Michael Windhal, Hui Xu
  • Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
    Patent number: 10577634
    Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: March 3, 2020
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes, Alex Van Eck Conradie, Benjamin D. Herzog
  • Polypeptides having xylanase activity and polynucleotides encoding same
    Patent number: 10570384
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: February 25, 2020
    Assignee: Novozymes, Inc.
    Inventor: Nikolaj Spodsberg
  • Method for producing L-amino acids
    Patent number: 10563234
    Abstract: A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: February 18, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Hidetaka Doi, Akiko Matsudaira, Yoshihiro Usuda
  • Xylanase
    Patent number: 10550376
    Abstract: Provided is a novel xylanase having high xylanase activity. A protein consisting of an amino acid sequence described in the following (a), (b) or (c) and having xylanase activity: (a) the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2; (b) an amino acid sequence having at least 90% identity to the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2; (c) an amino acid sequence modified from the amino acid sequence set forth in positions 23 to 404 of SEQ ID NO: 2 by deletion, insertion, substitution, or addition of one or more amino acids.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: February 4, 2020
    Assignee: Kao Corporation
    Inventors: Nozomu Shibata, Mari Suetsugu
  • System and method for creating crystals of human acetylcholinesterase with open active sites
    Patent number: 10544421
    Abstract: A system and method for creating crystals from human acetylcholinesterase. The crystals can then be analyzed using X-ray crystallography. A segment of DNA for human acetylcholinesterase is isolated that includes the codon sequence GAGGGC. A polyhistidine tag is inserted between codon GAG and codon GGC to produce a recombinant acetylcholinesterase construct. The recombinant acetylcholinesterase construct is used to transfect cells on a prepared growth medium. The growing cells secrete a portion of the recombinant acetylcholinesterase construct. The secreted portion is concentrated and the tag cleaved. The concentrate is then buffered and used to form crystals.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: January 28, 2020
    Assignee: New York Structural Biology Center
    Inventor: Jonah Cheung
  • ?3 fatty-acid desaturase and method for producing eicosapentaenoic acid
    Patent number: 10538793
    Abstract: Provided is ?3 desaturase having high enzymatic activity even at normal temperature. A polypeptide which consists of an amino acid sequence having an identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 2 and has ?3 desaturation activity on C20 fatty acid, and a gene thereof.
    Type: Grant
    Filed: December 24, 2015
    Date of Patent: January 21, 2020
    Assignees: KYOTO UNIVERSITY, NISSHIN PHARMA INC.
    Inventors: Jun Ogawa, Akinori Ando, Eiji Sakuradani, Sakayu Shimizu, Shigeru Hiramoto, Masataka Harata, Yuki Takemoto
  • Site-specific polyethylene glycolylated (pegylated) asparaginase, preparation method therefor and use thereof
    Patent number: 10537620
    Abstract: The present application discloses a PEGylated asparaginase and use thereof. In this application, the polyethylene glycol (PEG) is coupled to the N-terminal amino of 1 or 2 subunits of L-asparaginase, and the molecular weight of the PEG is 30-40 KDa. The PEG is preferably branched and has an aldehyde group serving as an activating group. The PEGylated asparaginase is useful in the preparation of anti-tumor drugs.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: January 21, 2020
    Assignees: ZONHON BIOPHARMA INSTITUTE INC., GENSUN INSTITUTE OF BIOMEDICINE CO., LTD.
    Inventors: Bruce Yong Ma, Jun Wang, He Wang, Chunlin Xu, Yifei Chen, Yaofang Wang
  • Method for secretory production of protein
    Patent number: 10538798
    Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, as well as a method for secretory production of a heterologous protein. A coryneform bacterium is described that has been modified to have a specific mutation so as to harbor a phoS gene, and when cultured, is able to to produce a heterologous protein by secretory production.
    Type: Grant
    Filed: October 23, 2017
    Date of Patent: January 21, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yoshihiko Matsuda, Yumi Ito, Yukari Kashima, Naoko Yamada, Noriko Tsurui, Hiroshi Itaya
  • Recombinantly produced neutral protease originating from Paenibacillus polymyxa
    Patent number: 10526594
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: January 7, 2020
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing L-amino acid using the same
    Patent number: 10526586
    Abstract: The present disclosure relates to a novel pyruvate dehydrogenase variant, a polynucleotide encoding the pyruvate dehydrogenase variant, a microorganism of the genus Corynebacterium producing L-amino acid, which includes the pyruvate dehydrogenase variant, and a method for producing an L-amino acid using the microorganism.
    Type: Grant
    Filed: March 15, 2016
    Date of Patent: January 7, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Lan Huh, Jun Ok Moon, Hyun Won Bae, Hyung Joon Kim, Sung Ki Song
  • Stabilized alpha-amylase variants and use of the same
    Patent number: 10519405
    Abstract: The present invention relates to alpha-amylase variants having an improved stability as compared to the parent alpha-amylase. The invention further relates to use of the variants, compositions comprising the variants, and methods of producing the variants.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: December 31, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Carsten Andersen, Anna-Kathrine Fevre
  • Beta-hexosyl-transferases and uses thereof
    Patent number: 10513695
    Abstract: This invention relates generally to the discovery of novel recombinant forms of ?-hexosyl-transferases (BHT) and uses thereof to produce galacto-oligosaccharides (GOS) or as food additives.
    Type: Grant
    Filed: September 12, 2017
    Date of Patent: December 24, 2019
    Assignee: North Carolina State University
    Inventors: Jose M. Bruno-Barcena, Suzanne Dagher, Maria Azcarate-Peril
  • Method of producing lipid
    Patent number: 10508292
    Abstract: A method of producing a lipid, containing the steps of culturing a transformant obtained by introducing a gene encoding the following protein (a) or (b) into cyanobacteria, and producing a lipid: (a) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (b) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence of the protein (a), and having ?-ketoacyl-ACP synthase activity.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: December 17, 2019
    Assignee: Kao Corporation
    Inventors: Akihito Kawahara, Shinji Sugihara
  • Gamma glutamyl-valine synthase, and method for producing gamma glutamyl-valyl-glycine
    Patent number: 10508295
    Abstract: ?-Glu-Val synthetase suitable for generating ?-Glu-Val, and a method for producing ?-Glu-Val-Gly using the same are provided. By using ?-Glu-Val synthetase showing a ratio of ?-glutamylvaline synthetase activity to ?-glutamylglycine synthetase activity of 2.0 or higher, such as ?-Glu-Val synthetase of Kocuria rosea (AJ3132), ?-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: December 17, 2019
    Assignee: AJINOMOTO CO., INC.
    Inventors: Chihiro Tsuji, Hiroyuki Nozaki
  • Production of xylitol from glucose by a recombinant strain
    Patent number: 10494614
    Abstract: The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding an NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding an NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: December 3, 2019
    Assignee: ROQUETTE FRERES
    Inventors: Astrid Schaefer, Melanie Diefenbacher, Yiming Chang, Sumire Honda Malca, Markus Schwab, Sophie Defretin, Tania Gerard, Arnaud Heysen, Friederike Thor
  • 3-alpha-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid
    Patent number: 10487348
    Abstract: The invention relates to novel 3-hydroxysteroid dehydrogenase mutants, to the sequences which code for these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic conversions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCA); subject-matter of the invention is also novel processes for the synthesis of UDCA using enzyme mutants; and the preparation of UDCA using recombinant, multiply modified microorganisms.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: November 26, 2019
    Assignee: PharmaZell GmbH
    Inventors: Werner Hummel, Daniel Bakonyi
  • Lyase and method for asymmetric synthesis of (S)-phenylacetylcarbinol
    Patent number: 10465211
    Abstract: A lyase has an amino acid sequence selected from SEQ ID NOs: 1, 2 and 3, wherein the amino acid isoleucine in position no. 468 in the protein ApPDC-E469G, which is modified with respect to the wild type from Acetobacter pasteurianus, is replaced by an amino acid which occupies less space than isoleucine.
    Type: Grant
    Filed: August 27, 2015
    Date of Patent: November 5, 2019
    Assignee: FORSCHUNGSZENTRUM JUELICH GMBH
    Inventors: Doerte Rother, Martina Pohl, Torsten Sehl, Lisa Marx, Robert Westphal
  • Tyrosine hydroxylase variants and methods of use thereof
    Patent number: 10457918
    Abstract: The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.
    Type: Grant
    Filed: September 24, 2015
    Date of Patent: October 29, 2019
    Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, VALORBEC S.E.C.
    Inventors: Vincent J. J. Martin, Lauren Narcross, John E. Dueber, William C. DeLoache, Zachary N. Russ, P. James Scrivens