Abstract: A method for producing biogas includes: anaerobically digesting excrement with an additive, the additive including a pre-pupal stage of an insect from the scientific classification superfamily of Stratiomyoidea; and collecting the biogas. A method for producing an additive for anaerobic digestion of excrement includes: hatching eggs of an insect from the scientific classification superfamily of Stratiomyoidea in excrement; growing insect larvae by feeding with additional excrement; harvesting pre-pupal insect larvae; and grinding the pre-pupal larvae. An apparatus for producing an additive for anaerobic digestion of excrement includes: a tray having pivot along one lateral side and an upwardly sloping wall terminating with a lip along a lateral side opposite the pivot; a flume adjacent the lip; and a conveyor adjacent the pivot.

Abstract: The invention provides a fermented Pennisetum extract, which has an advantageous effect in treating and preventing a cancer without toxicity to normal cells. The invention also provides a process of preparing the fermented Pennisetum extract and the application of the fermented Pennisetum extract in the treatment or prevention of a cancer.

Abstract: A chromogenic absorbent material for an animal litter includes an oxidizing agent responsive to peroxidatic/pseudoperoxidatic activity in an animal excretion or a first catalytic compound generating the oxidizing agent in situ. The material also includes a chromogenic indicator being chromogenically responsive to the oxidizing activity of the oxidizing agent, and an absorptive material which is porous, for absorbing the animal excretion. The absorptive material includes a water-absorbing polysaccharide providing absorptive properties to the chromogenic absorbent material; and may also include a second polysaccharide and a superabsorbent polymer. The material may be obtained in the form of particles having a low density and a high porosity, and is usable in conjunction with an animal litter for detecting various diseases in animals.

Abstract: An apparatus and a method for the automated processing of samples containing biological cells—in particular, of blood samples or other cell samples is provided. The apparatus has a sample receiving device configured to receive samples, an auxiliary material receiving device configured to receive auxiliary materials, a sample carrier receiving device configured to receive sample carriers, a discharge device configured to discharge samples from a discharge position, and a capture device configured to capture discharged samples in a capture position to obtain prepared samples, and at least one temperature control device for controlling the temperature of at least one of the sample carriers. The distance between the discharge position and the capture position can be adjusted to a prespecified height. The method includes wetting a temperature-controlled sample carrier with at least one auxiliary material and discharging the sample onto the wetted temperature-controlled sample carrier from a prespecified height.

Abstract: Disclosed are methods and systems for propagating microorganisms such as yeast using a carbon source that includes glycerol and a nutrient source that includes a stillage component.

Abstract: In one aspect, a method for feeding animals includes selecting a plurality of strains of sporulated living microbes and form a microbial solution; and applying the microbial solution to a carrier. In another aspect during use, the method includes feeding animals with the microbial infused carrier to deliver microbes to a gastrointestinal tract alive; and germinating the microbes in the digestive tract and stimulating local intestinal immunity with the microbes.

Abstract: The present invention provides methods of synthesizing trehalose analogues; methods of detecting mycobacteria, and trehalose analogues.

Abstract: The invention provides novel carbohydrate esters, in particular disaccharide esters, and the methods of their preparation. These compounds find use as microbial media components for the induction of gene expression in microbial fermentation processes.

Abstract: The present disclosure provides distinct therapeutic populations of cells that form a pharmaceutical composition useful in hematopoietic stem/progenitor cell transplant. For example, the present disclosure provides a therapeutic population of cells, comprising an enriched population of hematopoietic stem/progenitor cells, memory T cells, regulatory T cells, and wherein the population of cells is depleted of naïve conventional ??-T cells. The present disclosure further provides methods of treatment using the therapeutic population of cells. In other embodiments, the present disclosure provides methods of producing a therapeutic population of cells.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences especially for use as to nucleotide repeat disorders. Provided are delivery systems and tissues or organ which are targeted as sites for delivery especially for use as to nucleotide repeat disorders. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex or system especially for use as to nucleotide repeat disorders, as well as methods for the design and of such. Also provided are methods of directing CRISPR complex or system formation in eukaryotic cells especially for use as to nucleotide repeat disorders including with consideration of specificity for target recognition and avoidance of toxicity and editing or modifying a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: Disclosed are methods of diagnosing constipation and constipation-predominant irritable bowel syndrome by screening for the presence of abnormally high levels of methane and/or methanogenic organisms in a subject.

Abstract: Provided is a preparation method for olfactory ensheathing cells. The method comprises the steps of formulation of a cell medium, collection and pretreatment of tissues, enzymatic digestion, cell culture, cryopreservation, and differentiation culture. The prepared olfactory ensheathing cells can maintain the proliferative capacity for a long time, and still possess the activity of olfactory ensheathing cells after 11th passage.

Abstract: Compositions and methods use Bifidobacterium longum NCC3001 (ATCC BAA-999) to treat or prevent a depressive symptom. Prolonged anti-depressive effects can continue after administration of the compositions is ended. Non-limiting examples of a depressive symptom which can be treated or prevented include depressed mood; sadness; anxiety; “empty” feelings; loss of interest or pleasure; irritability; restlessness; changes in appetite or weight; sleep disturbances; lack of or decreased energy; feelings of worthlessness; guilt; helplessness; anger and hostility; difficulty in thinking, concentrating, or making decisions; hopelessness; tiredness; fatigue; memory difficulties; tearfulness; brooding; phobias; excessive worry over physical health; sexual dysfunction; persistent physical symptoms that do not respond to treatment; and combinations thereof.

Abstract: The invention relates to a process for preparing unipolar cation-conducting ionomers from fluoro ionic monomers, to said unipolar cation-conducting ionomers, to the uses thereof, to an electrolytic composition comprising at least one of said unipolar cation-conducting ionomers and to an electrochemical device comprising at least one of said unipolar cation-conducting ionomers, especially as electrolyte.

Abstract: Drug-delivery systems are described which can serve as platforms for topical delivery of a variety of active pharmaceutical ingredients (API) to the surface of a mammalian body. Methods for the preparation and use of the compositions are also described. In some embodiments, the disclosed topical formulations include an API, a penetration enhancer/cosolvent, a gelling agent, and a neutralizing agent (to adjust pH). As compared to prior compositions, the disclosed topical formulations use a relatively small number of safe components and are easy to prepare with a high yield of finished product.

Abstract: The invention relates to the field of blood clotting diagnosis and relates to a kinetic method for determining the fibrinogen concentration in a human plasma sample according to the Clauss method.

Abstract: The present disclosure provides for a cryopreservation composition comprising a permeating cryoprotectant, a saccharide and a macromolecule. The cryopreservation composition has a low toxicity to cells and tissues, and promotes survival and retention of viability of the biological material during cryopreservation.

Abstract: The present invention provides compositions and methods for prevention, amelioration and treatment of gram positive bacteria, particularly Staphylococcal bacteria, with combinations of lysin, particularly Streptococcal lysin, particularly the lysin PlySs2, and one or more antibiotic, including daptomycin, vancomycin, oxacillin, linezolid, or related antibiotic(s).

Abstract: A method for culturing and preserving the probiotic gut microorganism Eubacterium hallii is disclosed. The method provides an E. hallii preparation that is suitable for administration or ingestion to humans and that provides high E. hallii biomass yields. Preferably, the medium used for culturing E. hallii to high biomass yields comprises food grade components only, is free of any animal sources, and/or is kosher. Further, including a method of treating disease using the E. hallii preparation.

Abstract: A fluid pumping and bioreactor system including at least two cassettes, at least one storage reservoir, at least one bioreactor, at least one manifold including valve modules, and tubing to connect the cassettes to the storage reservoir and the bioreactor. The cassettes can include pumps, valves, and fluid conduits and can be communicatively connected to the at least one manifold. The bioreactor can include an adapter and fluid conduits extending through the adapter from the exterior of the bioreactor to the interior of the bioreactor. System and method for generating a tissue for transplant by decellularizing and recellularizing a supplied tissue.