Abstract: Methods for treating tissue matrices and tissue matrices produced according to the methods are provided. The methods can include treating a tissue matrix with a proteolytic enzyme to produce a desired pliability of the tissue matrix.

Abstract: The invention relates to a method and an apparatus for hydrolyzing and acidifying homogenized organic waste fed into a closed and gas tight reactor using enzymatic bacteria under thermophilic conditions where water may be added to said reactor and the content of the reactor can be mixed and subjecting during hydrolysis and acidification an adequate part of a suspension formed in the reactor to—i. a separation step isolating—a permeate comprising an aqueous solution of soluble carbon, volatile fatty acids (VFA) and valuable nutrients, —an organic slurry comprising an aqueous solution of insoluble organic solids rich in enzymatic thermophilic bacteria and non-hydrolyzed organic solids, and the remaining part of soluble carbon, VFA and valuable nutrients, thereafter to—ii. a recirculation step re-feeding said organic slurry into the reactor for further hydrolysis and acidification.

Abstract: A method of preparing a bone graft. Mixing bone marrow aspirate with an effective amount of a binding reagent that is capable of binding with red blood cells in the bone marrow aspirate. The bound red blood cells are aggregated. The aggregated bound red blood cells are separated from the bone marrow aspirate to provide a supernatant. At least a portion of the supernatant is associated with an osteoconductive matrix to form the bone graft.

Abstract: Methods of making and osteoinductive compositions are provided. The method includes mixing isolated periosteum tissue with a demineralized bone matrix to form the osteoinductive composition. The periosteum tissue is isolated from the surface of large bones such as femur, tibia, humerus or a mixture thereof. The demineralized bone matrix can include a plurality of surface demineralized or fully demineralized bone fibers, bone chips, bone particles or mixtures thereof. In the osteoinductive composition the substantially isolated periosteum tissue is from about 0.5% wt. to about 10% wt. A method for repairing a bone defect is also provided. The method of treatment includes applying to the bone defect a substantially effective amount of a mixture of isolated periosteum tissue and demineralized bone matrix.

Abstract: The invention provides compositions comprising mesenchymal stem cell (MSC) derived exosomes, and methods of their use in subjects having certain lung diseases including inflammatory lung disease.

Abstract: Provided are: a method for preparing a decellularized tissue product in which decellularized tissue can be filled with liquid while changes in the structure of support tissue constituting the decellularized tissue are inhibited; and a graft provided with a decellularized tissue product. The method for preparing a decellularized tissue product includes a reduced-pressure step for bringing an animal-derived decellularized tissue material and a liquid into contact under reduced-pressure conditions, and/or a pressurized step for bringing same into contact under pressurized conditions. The graft is provided with a decellularized tissue product prepared by the method of preparation.

Abstract: Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets.

Abstract: The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

Abstract: The process of growing a homogeneous polymer matrix comprising the steps of growing a viable mycelium in a liquid suspension; extracting mycelium from the liquid suspension; thereafter incubating the mycelium for a period of time sufficient to induce mycelium cohesion and to form a solid material; and thereafter drying the solid material to remove moisture and to inactivate the mycelium.

Abstract: Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets.

Abstract: The present invention relates to a method for microbial fermentation and biotransformation of aromatic acids to aromatic acids with reduced carbon atoms of wide commercial importance using a culture of actinomycete species. Amycolatopsis sp or the mutant thereof is employed in the present invention to convert natural as well as synthetic aromatic acids to reduced carbon aromatic acids with wide applications. The said culture in the disclosed invention is adapted to grow at 37-46° C. to achieve the biotransformation of aromatic acid to reduced carbon aromatic acid is accomplished at 37-46° C. to obtain a higher yield of the product.

Abstract: The present invention pertains to the development of biologically derived extracellular matrices (ECM) derived from decellularized pleura tissue. Such matrices are useful in many clinical and therapeutic applications, including the repair, reconstruction, sealing, or joining of tissue, tendons, bones, and/or ligaments. In addition, the present invention features methods of making a biologically derived ECM derived from decellularized pleura tissue. The invention further features laminated ECM matrices.

Abstract: The present invention is directed to methods of manufacturing bioactive gels from ECM material, i.e., gels which retain bioactivity, and can serve as scaffolds for preclinical and clinical tissue engineering and regenerative medicine approaches to tissue reconstruction. The manufacturing methods take advantage of a new recognition that bioactive gels from ECM material can be created by digesting particularized ECM material in an alkaline environment and neutralizing to provide bioactive gels.

Abstract: A cardiothoracic construct fabricated from human birth tissue comprising at least one cross-linked amniotic membrane, or at least one cross-linked chorionic membrane, or at least one amniotic membrane, or at least one chorionic membrane, or any combination thereof wherein the membrane(s) is/are treated with at least one alcohol composition followed by terminal sterilization is provided. Methods of processing a membrane to form a cardiothoracic construct and methods of preventing adhesion after a cardiac surgical procedure are also provided.

Abstract: Disclosed are a method of promoting hair growth or preventing hair loss in a mammal. In one aspect, the method includes administering interdermally or subcutaneously an agent containing an active ingredient comprising bFGF and a pharmaceutically acceptable substance, wherein bFGF is administered in a dose of 1 ?g to 100 ?g per 1 cm2 of the target area of hair growth treatment or hair loss prevention and a composition for use in the method.

Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

Abstract: A method includes administering a cosmetically or dermopharmaceutically effective quantity of exopolysaccharide of a bacterial strain isolated from Pseudoalteromonas sp. with deposit number CNCM I-4150 to at least one of skin, mucous membranes, hair and nails for the treatment and/or care of the skin, mucous membranes, hair and/or nails, and cosmetic and/or dermopharmaceutical compositions including the exopolysaccharide. In particular, the method and composition are suited to treatment of the aging of skin and for the treatment and/or care of disorders, conditions and/or diseases which are a result of a lack or decrease in hydration.

Abstract: A hemostatic device, method of making, and method of using for internal and external applications to wounds in the body of a patient to induce homeostasis at an anatomical site.

Abstract: A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

Abstract: The present invention is a composition of a self-assembly of collagen fibers made from a plurality of sized pieces of a source material. The source material made from animal or human tissue selected from one of dermis, tendon, fascia or dura mater. The self-assembly occurring by induction of a magnetic field in a liquid medium.