Abstract: Novel vectors are capable of producing MPMV (Mason-Pfizer Monkey Virus) proteins but not of packaging MPMV RNA, and the information about the packaging signal in MPMV and HIV can be used to create MPMV and HIV vectors that are capable of transferring foreign genes, e.g. for use in gene therapy.

Abstract: Novel PF 1022 derivatives--cyclodepsipeptides represented by the below-described formula (I)--and acid addition salts thereof, which have been synthesized according to the present invention, have anthelmintic activities against various parasitic worms which are parasitic on human bodies, domestic animals and pet animals. They are therefore useful as anthelmintics for the prevention or treatment of parasitic infections. ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4, Q, X, Y and Z have been defined herein.

Abstract: The invention relates to the clone pTOM36, comprising a gene encoding an enzyme involved in ripening related processes in tomato. Also described are DNA constructs comprising pTOM36 and a transcriptional intiation region operative in plants such that pTOM36 RNA is generated in plant cells, and tomato cells transformed with the DNA constructs.

Abstract: The invention relates to combinations of PKC-targeted (especially PKC-.alpha.-targeted) deoxyribo- and ribo-oligonucleotides and derivatives thereof with other chemotherapeutic compounds, as well as to pharmaceutical preparations and/or therapies, in relation to disease states which respond to such oligonucleotides or oligonucleotide derivatives, especially to to modulation of the activity of a regulatory protein.

Abstract: A method for making crystals of zinc interferon alpha-2, and the use thereof in depot formulations, are disclosed.

Abstract: Methods and compositions are described for the use of ribosomal RNA as a vehicle for heterologous sequences.

Abstract: The invention relates to recombinant DNA molecules comprising a Dictyostelium discoideum homologous promoter region, a heterologous DNA sequence with a Dictyostelium discoideum homologous peptide sequence capable of functioning as a leader peptide sequence positioned upstream thereof and in proper reading frame therewith, and a Dictyostelium discoideum homologous termination region, the heterologous DNA sequence encoding the desired functional polypeptide or intermediate thereof, said DNA sequence being positioned downstream from the promoter region and said termination region being positioned downstream from the DNA sequence. The invention also provides recombinant dictyostelid hosts as well as methods for preparing recombinant DNA molecules of the invention and for expressing recombinant polypeptides encoded by said recombinant DNA molecules in dictyostelid hosts. The invention in particular relates to the expression of the CS protein of Plasmodium falciparum.

Abstract: Rice plants are disclosed with two separate, but synergistic mechanisms for resistance to herbicides that normally inhibit a plant's acetohydroxyacid synthase (AHAS) enzyme. The herbicide resistance of plants with both resistance mechanisms is substantially greater than one would expect from a simple combination of the two types of resistance. The first of the two resistance mechanisms is a metabolic pathway that is not fully understood, but that does not itself involve a mutant AHAS enzyme. The second resistance mechanism is a mutant AHAS enzyme, an enzyme that shows direct resistance to levels of herbicide that normally inhibit the enzyme, in both in vivo and in vitro assays. Besides controlling red rice, many AHAS-inhibiting herbicides also effectively control other weeds that are common in rice fields. Several of these herbicides have residual activity, so that one treatment in the early spring controls both existing weeds as well as weeds that sprout later.

Abstract: The present invention is directed to a vector for transferring heterologous DNA into a plant cell. The vector is based on the bacterial artificial chromosome (BAC) vector designed for the construction of genomic libraries with large DNA inserts, and the binary (BIN) vector designed for Agrobacterium-mediated plant transformation. The BIBAC vector according to the subject invention allows the construction of plant genomic libraries with large DNA inserts that can be directly introduced into plants by transformation mediated by Agrobacterium.

Abstract: To make it possible to control the external view of flowers of petunias not by breeding but by only controlling cultivation environment, a method of cultivating a petunia having a colored portion and an outer margin in its corolla is proposed, which comprises controlling light quality of cultivation light in the daylight period after a seedling culture stage, in particular, after flower-bud differentiation until blooming to thereby control the proportion of the colored portion to the outer margin in the corolla. Especially, to broaden the outer margin in the corolla of the petunia, it is preferable to use yellow light as the cultivation light, and to narrow it, blue light as the light quality.

Abstract: A reagent, useful in the detection of environmental insults comprising bacterial cells containing a stress promoter operably linked to a lux gene complex has been prepared by lyophilizing the cells in a specified medium. The reagent may be used immediately upon rehydration where a positive test for the presence of an environmental insult is given by an increase in light production from the cells.

Abstract: An inbred broccoli line, designated SA-5, is disclosed. The invention relates to the seeds of inbred broccoli line SA-5, to the plants of inbred broccoli line SA-5 and to methods for producing a broccoli plant produced by crossing the inbred line SA-5 with itself or another broccoli line. The invention further relates to hybrid broccoli seeds and plants produced by crossing the inbred line SA-5 with another broccoli line.

Abstract: The invention provides a method for transforming a plant through a bacterium belonging to genus Agrobacterium, comprising transforming plant cells simultaneously with a first T-DNA (1) and a second T-DNA (2); and selecting the cells which acquired drug resistance; the first T-DNA (1) containing a gene giving the drug resistance, which functions in the plant; the second T-DNA (2) containing a desired DNA fragment to be introduced into the plant, the second T-DNA (2) being contained in a hybrid vector; the hybrid vector being prepared by homologous recombination between an acceptor vector and an intermediate vector in the bacterium belonging to genus Agrobacterium; the acceptor vector containing at least (a) a DNA region having a function to replicate a plasmid in the bacterium belonging to genus Agrobacterium and Escherichia coli, (b) a DNA region containing virB gene and virG gene in virulence region of Ti plasmid pTiBo542 of Agrobacterium tumefaciens, and (c) a DNA region which is homologous with a part of t

Abstract: We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target DNA has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into DNA targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

Abstract: This invention provides means for identifying maize plants having resistance to the European corn borer (ECB). Restriction fragment length polymorphism (RFLP) marker probes which are genetically linked to loci which condition ECB resistance are provided. These probes are useful in breeding programs to identify ECB resistant gene types and thus allow the resistance traits to be introduced into non-resistant or less resistant corn varieties.

Abstract: Beneficial endophytes which live within certain plants are known to provide desirable, cost-effective biological insect control. Many naturally occurring grasses host symbiotic endophytic fungi. However, beneficial endophytes have never been found in several species of turf grass, including species of bentgrasses and Kentucky bluegrasses, two commercially important turf grasses which are used extensively on golf courses and for lawn turfs. The invention of this application relates to new methods of inoculating plant tissues which allow the development of endophyte-enhanced varieties of turfgrasses, and to the turf grass varieties produced using the methods.

Abstract: The present invention is directed to a process for the production of a peptide, polypeptide, or protein having a predetermined property. In accordance with one embodiment, the process begins by producing by way of synthetic polynucleotide coupling, stochastically generated polynucleotide sequences. A library of expression vectors containing such stochastically generated polynucleotide sequences is formed. Next, host cells containing the vectors are cultured so as to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences. Screening or selection is carried out on such host cells to identify a peptide, polypeptide, or protein produced by the host cells which has the predetermined property. The stochastically generated polynucleotide sequence which encodes the identified peptide, polypeptide, or protein is then isolated and used to produce the peptide, polypeptide, or protein having the predetermined property.

Abstract: A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter.

Abstract: Biologically active, backbone-cyclized peptides of the formula: ##STR1## wherein ?AA! or ?A.sup.1 A.sup.1 ! is a naturally occurring or synthetic amino acid residue, n or e is an integer of 1-10, m or d is 0 or an integer of 1-10, R is a naturally occurring or synthetic amino acid side-chain, E is a hydroxyl moiety or a carboxyl protecting group of a blocking group, optionally covalently attached to an insoluble polymeric support, and the circled line designates a spacer group of ##STR2## for formula I wherein M is --S--S--, --CO--NH-- or --S-- and p and q each is an integer of 2-10, or--(CH.sub.2).sub.p --(M).sub.x --Y (IV)for formula II wherein M is an amino or carboxyl group or a sulfur atom, p is an integer of 2-10, x is 0 or 1 and Y is a side-chain of a backbone amino acid. Also, processes for the preparation of these peptides and pharmaceutical compositions containing them.

Abstract: The present invention provides methods of synthesizing cellulose in the storage tissue of transgenic plants by introducing the cellulose biosynthetic enzymes into the storage tissue. Specifically, the present invention involves introducing the genes for cellulose biosynthesis from the species Acetobacter xylinium into a given plant under the control of storage tissue specific promoters.