Patents Examined by Ethan C. Whisenant
  • Methods and compositions for isolating small RNA
    Patent number: 10619150
    Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: April 14, 2020
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventor: Richard Conrad
  • Reagents and methods for isothermal chain reaction
    Patent number: 10612077
    Abstract: In certain aspects, the invention disclosed herein relates to the isothermal amplification of probe linkage products to generate specific amplified signals. In some aspects, the invention provides methods, reagents, and kits for carrying out such amplification via the isothermal chain reaction (ICR).
    Type: Grant
    Filed: October 22, 2015
    Date of Patent: April 7, 2020
    Inventor: Ricardo Mancebo
  • Method for selecting a target nucleic acid sequence
    Patent number: 10612093
    Abstract: The present invention relates to a method of selecting a target region of interest (ROI) in a target nucleic acid molecule using a nucleic acid probe comprising sequences capable of directing the cleavage of a target nucleic acid molecule to release a fragment comprising the ROI and sequences capable of templating the circularisation and ligation of the target fragment. The circularised molecule thus obtained contains the selected ROI and may be subjected to further analysis and/or amplification etc. Also provided are probes and kits for use in such methods.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: April 7, 2020
    Assignee: NAVINCI DIAGNOSTICS AB
    Inventors: Ulf Landegren, Rachel Yuan Nong
  • Probes for improved melt discrimination and multiplexing in nucleic acid assays
    Patent number: 10612074
    Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: April 7, 2020
    Assignee: LUMINEX CORPORATION
    Inventors: Doug Whitman, Nicolas Arab, Chuck Collins
  • Unfolding proximity probes and methods for the use thereof
    Patent number: 10597701
    Abstract: The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: March 24, 2020
    Assignee: NAVINCI DIAGNOSTICS AB
    Inventors: Ulf Landegren, Rachel Yuan Nong, Ola Söderberg, Irene Helbing
  • High-throughput nucleotide library sequencing
    Patent number: 10590483
    Abstract: Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences, for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: March 17, 2020
    Assignee: AbVitro LLC
    Inventors: Francois Vigneault, Adrian Wrangham Briggs, Christopher Ryan Clouser, Stephen Jacob Goldfless, Sonia Timberlake
  • Methods and kits for analyzing DNA binding moieties attached to DNA
    Patent number: 10590467
    Abstract: A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: March 17, 2020
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ido Amit, David Lara-Astiaso, Meital Gury
  • High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
    Patent number: 10590461
    Abstract: A method of transferring material from a first microfabricated device to a second microfabricated device. At least one magnetic bead is loaded into at least one microwell of the first microfabricated device, where a plurality of cells are cultivated. The second microfabricated device is positioned such that the at least one microwell of the first array of microwells is aligned with at least one microwell of the second array of microwells. A magnetic field is applied so as to move the at least one magnetic bead contained in the at least one microwell of the first microfabricated device into the at least one microwell of the second microfabricated device. In this manner, at least one cell from the plurality of cells in the at least one microwell of the first microfabricated device is transferred to the at least one microwell of the second microfabricated device.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: March 17, 2020
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Alexander Hallock, Michael Sha
  • Methods for synchronizing nucleic acid molecules
    Patent number: 10584378
    Abstract: The methods, systems, kits and reagents provided herein relate to the synchronization of dephased/prephased nucleic acid molecules during sequencing reactions. The methods, systems, kits and reagents provided herein can be utilized to improve the efficiency and accuracy of sequencing technologies.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: March 10, 2020
    Assignee: Centrillion Technology Holdings Corporation
    Inventors: Filip Crnogorac, Wei Zhou
  • Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA
    Patent number: 10577645
    Abstract: Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human blood, plasma and/or serum sample. In an embodiment, the method comprises the step of selectively depleting 5?-RNAY4 fragments from the population of RNA molecules or selectively blocking 5?-RNAY4 fragments within the RNA population. The 5?-RNAY4 depleted or 5?-RNAY4 blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-486-5p fragments within the RNA population. The miR-486-5p depleted or miR-486-5p blocked population of RNA can also be used in global gene expression analysis protocols, including next generation sequencing.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: March 3, 2020
    Assignee: Norgen Biotek Corp.
    Inventor: Yousef Haj-Ahmad
  • Off-target capture reduction in sequencing techniques
    Patent number: 10577643
    Abstract: Presented herein are methods and compositions for enhancing specific enrichment of target sequences in a nucleic acid library. Off-target hybridization probes may be used to reduce binding and/or capture of off-target regions of a nucleic acid library in a targeted sequencing workflow. The off-target hybridization probes may be specific for locations known to generate off-target sequencing reads for a particular set of hybridization probes.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: March 3, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Li Teng, Chia-Ling Hsieh, Charles Lin, Han-Yu Chuang
  • Multi-species polynucleotide control sequences
    Patent number: 10577611
    Abstract: The present invention relates to polynucleotide sequences which enable a polynucleotide control sequence, such as a promoter, to direct expression in a wide range of industrially relevant species, both prokaryotes and eukaryotes. When the polynucleotide sequences of the invention are applied in combination with selection marker genes it is possible to perform selectable cloning in a laboratory host and use the same construct in the final host.
    Type: Grant
    Filed: April 10, 2014
    Date of Patent: March 3, 2020
    Assignee: Centrient Pharmaceuticals Netherlands, B.V.
    Inventor: Marco Alexander Van Den Berg
  • Method for modifying a template double stranded polynucleotide using a MuA transposase
    Patent number: 10570440
    Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: February 25, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventor: James White
  • Methods to determine conditions of a hydrocarbon reservoir
    Patent number: 10570735
    Abstract: A method of identifying in situ conditions of a hydrocarbon reservoir is disclosed. The method comprises, obtaining a sample from an area of interest, such as a sediment sample or water column sample near a hydrocarbon seep; analyzing the sample to detect nucleic acid signatures that are indicative of microbes associated with hypersaline aquifers; and using the signature to determine the salinity of the hydrocarbon reservoir.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: February 25, 2020
    Assignee: ExxonMobil Upstream Research Comapny
    Inventors: Aaron B. Regberg, Zarath M. Summers, A. Lucie N'Guessan
  • Single-molecule mechanoanalytical DNA device for ultrasensitive sensing of analytes
    Patent number: 10563247
    Abstract: A single-molecule mechanoanalytical real-time sensing device (SMART) comprising a molecular trawl and a DNA stem-loop structure that serves as a molecular dipstick, said trawl and said DNA stem-loop structure anchored by handles to two optically-trapped bead templates; said molecular trawl comprising multiple analyte recognition elements that exist in each of two separate DNA (pair) strands that act as two pincers, said pincers each having a nucleobase capable of catching an analyte in a media; said DNA stem-loop structure comprising a plurality of nucleotides in said loop and multiple base pairs in said stem; and wherein said DNA stem-loop is located generally opposite to said molecular trawl that is capable of reporting an amount of bound analyte target via mechanochemical transient events.
    Type: Grant
    Filed: April 20, 2017
    Date of Patent: February 18, 2020
    Assignee: KENT STATE UNIVERSITY
    Inventors: Hanbin Mao, Shankar Mandal
  • Nucleic acid target detection using a detector, a probe and an inhibitor
    Patent number: 10533212
    Abstract: The present invention generally pertains to methods for detecting the presence or absence of a particular nucleic acid sequence. The present invention generally relates to incorporating a detector into a target nucleic acid, adding an oligonucleotide probe, polymerase enzyme and an inhibitor to the reaction, and detecting interference of the oligonucleotide probe with the inhibitor as an indication of the presence of a particular target nucleic acid sequence as well as kits encompassing the same.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: January 14, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tal Raz, Pascaline Mary
  • Detection method and detection device
    Patent number: 10526644
    Abstract: A detection method for detecting a detection target substance is provided. The method includes a first application step of applying a magnetic field a liquid suspension to link a plurality of magnetic particles in a row from an interior surface of a liquid receiving member that receives the liquid suspension of magnetic particles to which a detection target substance is bound, a second application step of bringing the linked magnetic particles near the interior surface while the magnetic field is applied to the liquid suspension, and a detection step of detecting the detection target substance bound to the magnetic particles brought near the interior surface by the second application step, wherein the direction of the magnetic field applied to the liquid suspension is altered so the linked magnetic particles approach the interior surface in the second application step.
    Type: Grant
    Filed: September 28, 2016
    Date of Patent: January 7, 2020
    Assignee: SYSMEX CORPORATION
    Inventor: Kanako Nagaoka
  • Error correction of multiplex imaging analysis by sequential hybridization
    Patent number: 10510435
    Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: December 17, 2019
    Inventors: Long Cai, Sheel Shah, Eric Lubeck, Wen Zhou
  • Enzymatic ligation of nucleic acids
    Patent number: 10494671
    Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.
    Type: Grant
    Filed: January 17, 2012
    Date of Patent: December 3, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Stephen Hendricks, David King
  • Method for detecting detection object in sample, and detection kit using same
    Patent number: 10495633
    Abstract: The present invention relates to a novel method for detecting a detection object in a sample, and a detection device using the same. The detecting method of the present invention uses a “bridge composite” in which gold nanoparticles and an antibody specific to a detection object are coupled in order to induce a sufficient coupling reaction between the antibody and the detection object, thereby improving reactivity. Accordingly, since excellent resolution is provided, the method of the present invention has advantages of enabling accurate concentration measurement of a detection object in a sample, and amplifying a measurement signal. In addition, the method of the present invention can effectively detect small molecules such as hormones, vitamins, etc. having a small molecular weight.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: December 3, 2019
    Assignee: NANOENTEK, INC.
    Inventors: Joon Seok Seo, Kyu Hwan Shim