Abstract: Methods are provided for the generation of mesodermal cell types and derivatives thereof. Also provided are methods for generating purified populations of mesodermal cell types and derivatives thereof. The instant disclosure also provides methods of screening for cellular responses of the generated mesodermal cell types and derivatives thereof. Also provide are methods for screening for organismal phenotypes induced by introduction of the generated mesodermal cell types and derivatives thereof. Treatment methods making use of the generated mesodermal cell types and derivatives thereof are also provided. The instant disclosure also provides systems, compositions, and kits for practicing the methods of the disclosure.
Type:
Grant
Filed:
March 2, 2016
Date of Patent:
September 29, 2020
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Kyle Ming Loh, Irving L. Weissman, Lay Teng Ang, Angela Chen
Abstract: Blood brain barrier (BBB) permeabilizers, such as mannitol, can facilitate the entry of stem cells from the periphery to the stroke brain. It is unknown whether BBB permeation in the chronic stage of the disease still facilitates the entry of stem cells from the periphery to the injured brain. Evidence herein shows BBB permeation in the chronic stage of stroke assisted in the entry of stem cells from the periphery to the stroke brain. Stroke models treated with human umbilical cord stem cells (hUCBC) only (2 million viable cells), mannitol or a combination. Results revealed that hUCBC alone or combined with mannitol displayed significant behavioral and histological deficits compared to control animals, with the HUCBC-mannitol combined treatment showing improvements over hUCBC only treatments in brain cell survival in the peri-infarct area. BBB permeation in chronic stroke also lowers the effective stem cell dose necessary to improve functional outcomes.
Type:
Grant
Filed:
September 6, 2016
Date of Patent:
September 8, 2020
Assignee:
University of South Florida
Inventors:
Cesario Venturina Borlongan, Paul R. Sanberg
Abstract: The present invention relates to substantially homogenous populations of human retinal progenitor cells having the following positive surface markers: SSEA4, CD73, PTK7 and PSA-NCAM. The invention also relates to method for preparing such substantially homogeneous cell populations from human tissue using cell sorting techniques.
Abstract: A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.
Type:
Grant
Filed:
May 9, 2017
Date of Patent:
September 1, 2020
Assignee:
Vivex Biologics Group, Inc.
Inventors:
Timothy Ganey, Wendy W. Weston, Miguel Quevedo, Stuart Oglesby, Gaetan Jean-Robert Delcroix, Paul C. Schiller, Gianluca D'Ippolito
Abstract: The present invention provides a method for producing a retinal pigment epithelial cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and (3) a third step of subjecting the aggregate formed in step (2) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and a substance acting on the BMP signal transduction pathway and containing a substance acting on the Wnt signal pathway, thereby obtaining an aggregate containing retinal pigment epithel
Type:
Grant
Filed:
October 2, 2014
Date of Patent:
September 1, 2020
Assignees:
SUMITOMO CHEMICAL COMPANY, LIMITED, RIKEN
Abstract: A three-dimensional cell culture method on the basis of a biodegradable synthetic hydrogel is provided. The method may include culturing cells in a cell culture dish in a non-contact manner using a porous membrane and a biodegradable synthetic hydrogel in the culture of stem cells and primary cells.
Type:
Grant
Filed:
September 15, 2015
Date of Patent:
August 25, 2020
Assignee:
CEFO CO., LTD
Inventors:
Hyun Sook Park, Sun Ray Lee, Ji Won Yang, Seol Chu, Jang Mi Park, Hyun Jung Mo
Abstract: Methods for the recovery of lipids, sugars, and proteins from microbial biomass by enzymatic digestion are disclosed. The methods involve treating microalgae with a fungal acid protease, or with a mixture of at least one protease and at least one amylase.
Abstract: A method of making hydrolyzed marine Type II collagen includes the mixing of marine cartilage, water, an enzyme and a protease enzyme for an extended period of time. Once mixed, the mixture is heated for a period of time at 150° F. Once heated, the enzymes are deactivated, the bone sediment separated, and the fat removed. Next, maltodextrin is added to the mixture and finally the mixture is spray dried to form a collagen powder.
Abstract: The present disclosure provides methods, and related kits, for directing cell attachment and spreading on a substrate and inducing isotropic spreading of cells; provides methods, and related kits, for cell sorting; and further provides methods, and related kits, for guided induction of stem cell differentiation.
Abstract: Disclosed are isolated human bone marrow mesenchymal stem cells having high telomerase activity (tBMMSCs). Also disclosed are isolated human CD34+ bone marrow mesenchymal stem cells. Also disclosed are bone marrow mesenchymal stem cells treated with a telomerase induction agent.
Abstract: Disclosed herein is a skin external composition containing a red yeast rice extract. More particularly, disclosed is a skin external composition containing a red yeast rice extract which can increase the production of loricrin in keratinocytes and to strengthen the skin barrier function, thereby exhibiting an excellent effect of improving skin moisturization.
Type:
Grant
Filed:
December 20, 2017
Date of Patent:
June 30, 2020
Assignee:
Amorepacific Corporation
Inventors:
Dong Won Choi, Hong Guen Park, Han Byul Kim, Jin Sup Shim, In Hye Ha, Youn Joon Kim, Byung Young Kang
Abstract: The invention provides an agent for improving circadian rhythm comprising cells of lactic acid bacterium or treated product thereof as an active ingredient.
Type:
Grant
Filed:
February 8, 2013
Date of Patent:
June 30, 2020
Assignees:
National Institute of Advanced Industrial Science and Technology, Sapporo Holdings Limited
Abstract: The present invention relates generally to the field of tissue engineering and in particular to the production of tissue films or cell matrices, which can be used as a living tissue substitute or an artificial tissue construct in tissue repair or replacement.
Abstract: The present invention provides compositions for treating soft tissue injuries comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix. Methods of making and using compositions comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix are also provided.
Type:
Grant
Filed:
November 11, 2015
Date of Patent:
February 25, 2020
Assignee:
ALLOSOURCE
Inventors:
Reginald L. Stilwell, Brent Atkinson, Yaling Shi, Brian Dittman
Abstract: The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.
Type:
Grant
Filed:
March 24, 2008
Date of Patent:
February 4, 2020
Inventors:
Heeyoung Lee, Hyunjin Yang, Jun Seok Lee, Ji Suk Choi
Abstract: A stamp and a method for transferring particles to cells of a cell culture. The stamp includes a body having upper and lower surfaces. The lower surface includes a recessed portion. A gel extends along the recessed portion of the lower surface of the body and including the particles patterned therein. The body is configured to move between a first position wherein the gel is isolated from the cells of the cell culture and a second position wherein the particles patterned in the gel communicate with the cells of the cell culture through diffusion.
Type:
Grant
Filed:
June 19, 2015
Date of Patent:
December 31, 2019
Assignee:
Wisconsin Alumni Research Foundation
Inventors:
David Beebe, Erwin Berthier, Mary Regier
Abstract: A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture is compatible with biologic function.
Type:
Grant
Filed:
May 10, 2017
Date of Patent:
December 24, 2019
Assignee:
Vivex Biologics Group, Inc.
Inventors:
Timothy Ganey, Wendy W. Weston, Miguel Quevedo, Stuart Oglesby, Gaetan Jean-Robert Delcroix, Paul C. Schiller, Gianluca D'Ippolito
Abstract: Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition of enzymatic reactions during the production of useful products. The methods also include saccharifying recalcitrance-reduced lignocellulosic biomass and adding an isomerization agent to the saccharified biomass. The saccharified biomass can be further converted into products by a microorganism.
Type:
Grant
Filed:
October 19, 2015
Date of Patent:
December 17, 2019
Assignee:
Xyleco, Inc.
Inventors:
Marshall Medoff, Thomas Craig Masterman, Michael W. Finn
Abstract: The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.
Type:
Grant
Filed:
August 22, 2014
Date of Patent:
December 10, 2019
Assignees:
SUMITOMO CHEMICAL COMPANY, LIMITED, RIKEN