Patents Examined by Ganapathirama Raghu
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Patent number: 12042529Abstract: The present invention provides a method of PEGylating a human truncated cystathionine ?-synthase protein containing a mutation of a cysteine to a serine at amino acid position 15 (htCBS C15S). The htCBS C15S was PEGylated with one of 5 kDa, 10 kDa, or 20 kDa NHS ester PEG molecules. In-process monitoring of the PEGylation process was used in the method to reduce levels of unPEGylated htCBS C15S and htCBS C15S with insufficient PEGylation. Administration of the PEGylated htCBS C15S had efficacy throughout the course of treatment for homocystinuria.Type: GrantFiled: April 5, 2022Date of Patent: July 23, 2024Assignees: The Regents Of The University Of Colorado, A Body Corporate, Travere Therapeutics Switzerland GmbHInventors: Tomas Majtan, Jan P. Kraus, Erez M. Bublil, Frank Glavin, Marcia Sellos-Moura
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Patent number: 12043853Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.Type: GrantFiled: July 15, 2021Date of Patent: July 23, 2024Assignee: DANISCO US INC.Inventors: Andrew Shaw, David A Estell, Eugene S Sadlowski, Hiroshi Oh, Louise Wallace, Ronaldus Wilhelmus Joannes Hommes, Sang-Kyu Lee
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Patent number: 12037317Abstract: In various embodiments methods of preparing hydroxybutyryl 3-hydroxybutyrate and related compounds are provided along with methods of use thereof.Type: GrantFiled: January 18, 2019Date of Patent: July 16, 2024Assignee: Buck Institute for Research on AgingInventor: Scott Michael Ulrich
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Patent number: 12037629Abstract: In an embodiment, the present disclosure pertains to an oleaginous bacterium. In some embodiments, the oleaginous bacterium includes lipids and at least one exogenous and inducible gene. In some embodiments, the exogenous and inducible gene encodes at least one protein capable of inducing lysis in the oleaginous bacterium to release the lipids. In an addition embodiment, the present disclosure pertains to a method of releasing lipids into an environment. In general, the method includes one or more of the following steps of: (1) introducing at least one oleaginous bacterium of the present disclosure to the environment; and (2) inducing expression of at least one exogenous gene in the oleaginous bacterium to thereby induce the expression of at least one protein. In some embodiments, the protein facilitates the lysis of the oleaginous bacterium and release of the lipids into the environment.Type: GrantFiled: August 20, 2021Date of Patent: July 16, 2024Assignee: The Texas A&M University SystemInventors: Kung-Hui Chu, Ry Young, Jason Gill
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Patent number: 12031129Abstract: Methods and compositions for modulating a target genome are disclosed. The composition may comprise a first RNA encoding a polypeptide comprising a retrotransposase reverse transcriptase domain and a retrotransposase endonuclease domain. The composition may also comprise a second RNA comprising a sequence that binds the polypeptide and a heterologous object sequence. The composition may insert the sequence of the heterologous object sequence into a target DNA.Type: GrantFiled: December 6, 2019Date of Patent: July 9, 2024Assignee: Flagship Pioneering Innovations VI, LLCInventors: Jacob Rosenblum Rubens, Geoffrey A. von Maltzahn, Robert James Citorik, Barrett Ethan Steinberg, Donghui Li, William Edward Salomon
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Patent number: 12024727Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.Type: GrantFiled: March 5, 2021Date of Patent: July 2, 2024Assignee: Metagenomi, Inc.Inventors: Brian Thomas, Christopher Brown, Rose Kantor, Audra Devoto, Cristina Butterfield, Lisa Alexander, Daniela S. A. Goltsman, Jason Liu
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Patent number: 12018237Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: May 17, 2022Date of Patent: June 25, 2024Assignee: Novozymes A/SInventors: Carsten Andersen, Vivek Srivastava, Padmavathi Balumuri, Partha Pratim Chakrabarti, Sohel Dalal
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Patent number: 12018285Abstract: The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.Type: GrantFiled: December 6, 2022Date of Patent: June 25, 2024Assignee: Boehringer Ingelheim International GmbHInventors: Markus Mueller, Jochen Schaub, Christian Bernloehr, Jennifer Koenitzer
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Patent number: 12018301Abstract: The present disclosure relates to recombinant polypeptides that have olivetolic acid cyclase activity, nucleic acids encoding these recombinant polypeptides, recombinant host cells that produce these recombinant polypeptides, and compositions comprising the recombinant polypeptides, nucleic acids, and/or recombinant host cells. The present disclosure also relates to uses of these recombinant polypeptides, nucleic acids encoding them, and recombinant host cells comprising them, in methods for the preparation of cannabinoids and cannabinoid precursors.Type: GrantFiled: June 20, 2023Date of Patent: June 25, 2024Assignees: WILLOW BIOSCIENCES, INC., EPIMERON USA, INC.Inventors: Trish Choudhary, Xueyang Feng, Thanh Nguyen, Matthew Workentine
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Patent number: 12006528Abstract: Geranyl pyrophosphate (GPP) is a key intermediate molecule in the bioproduction of thousands of natural products. Currently, natural products are either cultivated from plants, synthesized via complex chemical synthesis strategies, or through cell-based factories also known as biofoundries. However, in order to replicate the process in a cell free environment, numerous enzymes and cofactors must be utilized making this approach costly and unviable. In order to make this process viable, a new approach was needed that uses fewer enzymes and co-factors. As described herein, the present invention demonstrates that it is possible to create GPP from glycerol through a short and concise biosynthetic pathway outside of the cell.Type: GrantFiled: December 17, 2021Date of Patent: June 11, 2024Assignee: DEBUT BIOTECHNOLOGY, INC.Inventors: Joshua Britton, Nicholas Brideau
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Patent number: 11999764Abstract: The present invention relates to a new class of sucrose esters and a method for their preparation.Type: GrantFiled: May 31, 2022Date of Patent: June 4, 2024Assignee: Julius-Maximilians-Universitaet WuerzburgInventors: Christian Possiel, Juergen Seibel
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Patent number: 11998027Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.Type: GrantFiled: June 1, 2022Date of Patent: June 4, 2024Assignee: ERBER AKTIENGESELLSCHAFTInventors: Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
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Patent number: 11998028Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.Type: GrantFiled: June 1, 2022Date of Patent: June 4, 2024Assignee: ERBER AKTIENGESELLSCHAFTInventors: Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
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Patent number: 11987825Abstract: The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.Type: GrantFiled: June 2, 2021Date of Patent: May 21, 2024Assignee: Danisco US Inc.Inventors: Marc Kolkman, Rie Mejldal, Anja Hemmingsen Kellett-Smith, Lilia Maria Babe
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Patent number: 11981945Abstract: The present invention relates to a process of producing a monoterpene and/or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and/or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and/or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and/or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.Type: GrantFiled: April 23, 2021Date of Patent: May 14, 2024Assignee: C3 BIOTECHNOLOGIES LIMITEDInventors: Vijaykumar Karuppiah, Nicole G. H. Leferink, Nigel S. Scrutton
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Patent number: 11965196Abstract: The disclosure relates to an enzymatic preparation method of inclusion complexes of tributyrin, and belongs to the technical field of oil microencapsulation. The disclosure combines enzymatic synthesis of cyclodextrin and inclusion of tributyrin with cyclodextrin, including enzymatic preparation of cyclodextrin with a CGT enzyme. Tributyrin is added in the preparation process; after reaction, Tween is added, and homogenization and spray drying are carried out. The effect of the finally obtained tributyrin powder is much better than that of single inclusion of tributyrin with cyclodextrin. The disclosure is simple in process, low in cost and convenient in operation; reaction processes are free of toxicity and pollution; there are no toxic reagent residues; the inclusion effect is obvious, and better utilization of a nutritional additive tributyrin in actual production is facilitated.Type: GrantFiled: September 28, 2020Date of Patent: April 23, 2024Assignee: JIANGNAN UNIVERSITYInventors: Caiming Li, Zhaofeng Li, Shuangdi Chen, Zhengbiao Gu, Yan Feng, Zexi Li, Li Cheng, Yan Hong
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Patent number: 11965194Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.Type: GrantFiled: July 21, 2022Date of Patent: April 23, 2024Assignee: Codexis, Inc.Inventors: Jack S. Liang, Stephan Jenne, Emily Mundorff, Rama Voladri, James J. Lalonde, Gjalt W. Huisman
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Patent number: 11965172Abstract: Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.Type: GrantFiled: November 4, 2019Date of Patent: April 23, 2024Assignee: California Institute of TechnologyInventors: Bruce A. Hay, Georg Oberhofer, Tobin William Ivy
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Patent number: 11959115Abstract: A saccharification reaction mixture can saccharify at least one of cellulose and hemicellulose and contains at least one of cellulose and hemicellulose, a saccharification enzyme, silica or a silica-containing substance, and at least one compound (A) selected from the group consisting of a polyhydric alcohol compound represented by the following formula (1) or a derivative thereof and an acetylene glycol represented by formula (2) or an alkylene oxide adduct thereof.Type: GrantFiled: May 11, 2022Date of Patent: April 16, 2024Assignee: NISSAN CHEMICAL CORPORATIONInventors: Kazutoshi Odaka, Kazutoshi Sekiguchi
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Patent number: 11939619Abstract: The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.Type: GrantFiled: March 30, 2022Date of Patent: March 26, 2024Assignee: CARGILL, INCORPORATEDInventors: Chris Miller, Brian Rush, Joshua Dunn, Brynne Stanton