Patents Examined by Ganapathirama Raghu
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Patent number: 11859221Abstract: The present invention relates to polypeptide having alpha-amylase activity. The present invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.Type: GrantFiled: June 30, 2022Date of Patent: January 2, 2024Assignee: Novozymes A/SInventors: Torsten Bak Regueira, Bitten Plesner, Thomas Holberg Blicher, Anne Dorte Houg, Sofia Arnehed, Lars Lehmann Hylling Christensen, Carsten Andersen
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Patent number: 11858965Abstract: The present disclosure relates to a recombinant protein comprising a plurality of type II cohesin repeats. The present disclosure provides a recombinant cellulosome complex comprising: the recombinant protein comprising a plurality of type II cohesin repeats; a recombinant cellulosome complex integrating protein A comprising a plurality of type I cohesin repeats, a plurality of cellulose-binding modules and a type II dockerin; and a plurality of recombinant enzymes each comprising a type I dockerin. A cell, a method for digesting a cellulose and a method for producing ethanol are also provided.Type: GrantFiled: May 21, 2021Date of Patent: January 2, 2024Assignee: ACADEMIA SINICAInventors: Wen-Hsiung Li, Anandharaj Marimuthu, Jui-Jen Chang, Yu-Ju Lin, Rizwana Parveen Rani Mohamed Gobil, Chieh-Chen Huang
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Patent number: 11851639Abstract: The present invention relates to compositions comprising protease variants suitable for use in cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: GrantFiled: October 14, 2021Date of Patent: December 26, 2023Assignee: NOVOZYMES A/SInventors: Peter Kamp Hansen, Esben Peter Friis, Torben Vedel Borchert, Lars Lehmann Hylling Christensen, Jens Erik Nielsen, Maria Berggaard Silow
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Patent number: 11844353Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C6-C24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The C6-C24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. Also provided are methods of producing unsaturated C6-C24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product alcohols, aldehydes, or acetates.Type: GrantFiled: August 20, 2021Date of Patent: December 19, 2023Assignee: Provivi, Inc.Inventors: Effendi Leonard, Peter Meinhold, Keith Wampler, Pedro Coelho, Micah Sheppard, Thomas Heel
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Phosphate-free automatic dishwashing detergent compositions having a protease and a complexing agent
Patent number: 11834634Abstract: A phosphate-free automatic dishwashing cleaning composition comprising i) a protease wherein the protease is a variant having at least 60% identity with the amino acid sequence of SEQ ID NO:1 and comprising at least one amino acid substitution (using the SEQ ID NO: 1 numbering) selected from the group consisting of X54T; X126A, D, G, V, E, K, I; X127E, S, T, A, P, G, C; and X128E, C, T, D, P, G, L, Y, N; and ii) from 10 to 50% by weight of the composition of a complexing agent system comprising from 0 to less than 30% by weight of the composition of citric acid.Type: GrantFiled: December 12, 2018Date of Patent: December 5, 2023Assignee: The Procter & Gamble CompanyInventors: Michelle Jackson, Eva Maria Perez-Prat de Vinuesa, David John Tarbit, Philip Frank Souter, Lilia Maria Babe, David Aaron Estell, Frits Goedegebuur, Harm Jan Mulder, Sina Pricelius, Lydia Dankmeyer, Thijs Kaper, Hatice Billur Engin -
Patent number: 11820988Abstract: Methods and nucleic acid sequences for the synthesis of biotemplates in a non-plant based expression system are provided. Such biotemplates include Barley stripe mosaic virus viral-like particles (BSMV-VLPs) that are capable of self-assembly due to being operatively linked with an origin of self-assembly with the Barley stripe mosaic virus capsid protein (BSMV-CP). Also provided are BSMV-VLPs that are capable of self-assembly due one or more site-directed mutations on the BSMV-CP, and BSMV-VLPs that exhibit enhanced stability due to such site-directed mutation(s).Type: GrantFiled: February 28, 2020Date of Patent: November 21, 2023Assignee: Purdue Research FoundationInventors: Kevin Solomon, Kok Zhi Lee, Yu-Hsuan Lee, Michael Harris, Loretta Sue Loesch-Fries
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Patent number: 11814659Abstract: Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.Type: GrantFiled: March 11, 2020Date of Patent: November 14, 2023Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Nicholas Stephanopoulos, Yang Xu
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Patent number: 11814656Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering.Type: GrantFiled: August 2, 2022Date of Patent: November 14, 2023Assignee: Novozymes A/SInventors: Carsten Hoerslev Hansen, Yitong Liu, Steffen Kayser, Vibeke Skovgaard Nielsen
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Patent number: 11814658Abstract: Methods to edit genes by administering a chimeric nuclease to a cell or organism without the use of a viral vector.Type: GrantFiled: November 15, 2022Date of Patent: November 14, 2023Assignee: Specific Biologics Inc.Inventors: David R. Edgell, Thomas A. McMurrough, Brent E. Stead, Odisho K. Israel
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Patent number: 11807668Abstract: A use of citronellol in preparing a preparation for promoting an expression of a virulence gene toxA of Pseudomonas aeruginosa is disclosed. It was found that citronellol slightly inhibits the growth of a Pseudomonas aeruginosa PAO1 strain and can promote the transcription of the toxA of Pseudomonas aeruginosa, which can increase the yield of an exotoxin A, namely, an encoded product of toxA. Therefore, citronellol is applicable to the preparation of a preparation for promoting the expression of the virulence gene toxA of Pseudomonas aeruginosa.Type: GrantFiled: October 27, 2021Date of Patent: November 7, 2023Assignee: INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICRORIInventors: Wenru Li, Xiaobao Xie, Taohua Zeng, Zhiqing Zhang, Jingxia Liu, Qingshan Shi
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Patent number: 11795418Abstract: The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: June 9, 2021Date of Patent: October 24, 2023Assignee: Novozymes A/SInventors: Jens Erik Nielsen, Lars Anderson, Allan Svendsen, Rune Nygaard Monrad, Lars Giger, Vasudeva Prahlada Rao, Sohel Dalal, Santhosh Vasu Mepadam, Marie Thrysoe Kruse, Kasper D. Rand, Pernille F. Jensen
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Patent number: 11795444Abstract: Provided are a composition for producing tagatose, comprising fructose-6-phosphate 4-epimerase, and a method of producing tagatose using the same.Type: GrantFiled: March 30, 2018Date of Patent: October 24, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Sung Jae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim
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Patent number: 11788070Abstract: In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3? carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.Type: GrantFiled: October 6, 2021Date of Patent: October 17, 2023Assignee: Manus Bio Inc.Inventors: Jason Eric Donald, Ajikumar Parayil Kumaran, Aaron Love, Christopher Toomey, Christine Nicole S. Santos
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Patent number: 11788095Abstract: The present invention relates to a synthetic promoter capable of controlling the expression of a target gene at various locations in the genome of an acid-resistant strain, and more particularly to a synthetic promoter including a core promoter derived from an acid-resistant strain and an upstream activating sequence (UAS) element serving as an enhancer. When the present invention is applied to a variety of genetic and metabolic engineering techniques for acid-resistant yeast, various metabolic networks can be configured as desired while controlling the expression level of the target gene, so a method of producing various metabolites using acid-resistant yeast is provided, and the cost of producing the metabolites can be greatly reduced depending on the properties of the acid-resistant yeast.Type: GrantFiled: November 10, 2021Date of Patent: October 17, 2023Assignee: SK Innovation Co., Ltd.Inventors: Jae Yeon Park, Ki Sung Lee, Tae Young Lee
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Patent number: 11773378Abstract: Described are a genetically modified microorganism and corresponding methods and products. The genetically modified microorganism may include a first gene that encodes an acyl transferase and a second gene that encodes a peptide or protein. One or both of the first and second gene may be heterologous. The genetically modified microorganism may include a modified acyl-CoA biosynthetic pathway configured for one or more of: inducible biosynthesis of an acyl-CoA and over-accumulation of the acyl-CoA. The genetically modified microorganism may be effective upon fermentation to cause acylation of the peptide or protein by the acyl transferase using the acyl-CoA to provide a N-acylated peptide or protein product.Type: GrantFiled: June 9, 2021Date of Patent: October 3, 2023Assignee: DUKE UNIVERSITYInventors: Michael David Lynch, Romel Menacho Melgar
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Patent number: 11773417Abstract: The present disclosure relates to strains of Thraustochytrium genus, including a high content of polyunsaturated fatty acids, and a method of producing a biomass using the same. According to the novel CJM01 microalgae of Thraustochytrium genus of the present disclosure, the content of lipids in the biomass and the content of unsaturated fatty acid such as docosahexaenoic acid in the biomass are high, so that the microalgae itself, a biomass produced by the culturing and fermentation of microalgae, a condensate of the biomass, and a dried product of the biomass are very useful as a feed composition.Type: GrantFiled: June 26, 2019Date of Patent: October 3, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Ji Young Kim, Myung Geun Park, Hye Min Park, Jung Woon Choi, Sang Min Park, Sang Young Bae, Jin Sook Chang
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Patent number: 11746369Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.Type: GrantFiled: April 26, 2021Date of Patent: September 5, 2023Assignee: Codexis, Inc.Inventors: Jeffrey C. Moore, Jack Liang, Jonathan Penfield, Jovana Nazor, Nikki Dellas, Vesna Mitchell, Da Duan, Iman Farasat, Agustina Rodriguez-Granillo, Grant Murphy, Nicholas Marshall
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Patent number: 11739312Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.Type: GrantFiled: December 2, 2020Date of Patent: August 29, 2023Assignees: BASF Enzymes LLC, Syngenta Participations AGInventors: Malgorzata Slupska, Geoff Hazlewood, Cathy Chang, Peter Luginbuhl, Ellen Burke, Michelle Cayouette, Uvini Gunawardena, Majid Ghassemian, Aron Silverstone, Yan Zhang
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Patent number: 11739357Abstract: The present invention provides a recombinant microorganism for producing citicoline and a method for producing citicoline by using the recombinant microorganism, wherein genes for degradation and utilization of citicoline, choline, and phosphocholine are knocked out, In addition, a pyrimidine nucleoside synthesis pathway is genetically engineered to remove feedback inhibition to the synthesis pathway. A yield of more than 20 g/L of citicoline can be obtained with recombinant strains in a 5-liter fermenter by means of a biological fermentation method, achieving industrial mass production with low citicoline production costs and less pollution; therefore, the method is a simple, environmentally friendly and has a relatively high promotion and application value.Type: GrantFiled: January 7, 2020Date of Patent: August 29, 2023Assignee: SUZHOU BIOSYNTHETICA CO., LTDInventors: Junjun Jiang, Junying Fan, Feng Tian, Xintong Wang, Kailin Zhang, Zhihao Hu
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Patent number: 11732281Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.Type: GrantFiled: September 2, 2021Date of Patent: August 22, 2023Assignee: BASF SEInventors: Brian Michael Green, Michelle Lorraine Gradley