Patents Examined by Ganapathirama Raghu
  • Polyphosphate-dependent glucokinase and method for preparing glucose 6-phosphate by using same
    Patent number: 10822629
    Abstract: The present invention relates to a novel high-temperature active thermoresistant polyphosphate-dependent glucokinase with high thermal stability, a composition including the enzyme, and methods for producing glucose 6-phosphate using the enzyme.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: November 3, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sung Jae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim, Seung Won Park
  • Deacetoxycephalosporin C hydroxylase mutants, DNA encoding the mutants, method for utilizing the mutants and application thereof
    Patent number: 10822631
    Abstract: This invention provides deacetoxycephalosporin C hydroxylase mutants, their encoding DNA sequences, the methods to utilize them and their application. The deacetoxycephalosporin C hydroxylase mutants are characterized by at least one amino acid mutation at residue position selected from Glycine at position 29, Alanine at position 40, Glycine at position 41, Arginine at position 182 and Threonine at position 272, based on the amino acid sequence shown in SEQ ID NO.:2 as reference sequence, and wherein the deacetoxycephalosporin C hydroxylase mutant has at least 10% increase in activity and at least 150% increase in thermostability compared to wild-type deacetoxycephalosporin C hydroxylase. The deacetoxycephalosporin C hydroxylase mutants in this invention have increased activity and thermostability, allowing them to be more commercially and industrially viable.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: November 3, 2020
    Assignee: BioRight Worldwide Co., Ltd.
    Inventors: Yuk Sun Lui, Shiu Ming Cheng, Yuen Wing Chow, Yau Lung Siu, Jun Wang
  • Polypeptides having endoglucanase activity and polynucleotides encoding same
    Patent number: 10815468
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: October 27, 2020
    Assignee: Novozymes, Inc.
    Inventors: Nikolaj Spodsberg, Tarana Shaghasi
  • Method for producing benzaldehyde
    Patent number: 10808264
    Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: October 20, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yasuaki Takakura, Keiko Danjo, Keita Fukui, Sayaka Asari, Takuto Ono
  • Non-caloric sweeteners and methods for synthesizing
    Patent number: 10800803
    Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: October 13, 2020
    Assignee: Conagen Inc.
    Inventors: Guohong Mao, Xiaodan Yu
  • Targeting of glycoprotein therapeutics
    Patent number: 10792342
    Abstract: Methods of making ligand-decorated polymer conjugates of therapeutic glycoproteins are described. Improved targeting of glycoproteins to specific tissues is achieved by masking the natural carbohydrate and other surface determinants with high molecular weight polymers, such as, e.g., PEG, polysialic acid, etc., which in turn are decorated with target-specific ligands. In some embodiments, acid-labile linkages in such conjugates or rapidly degradable masking groups allow for the intracellular release of the polymer from the glycoprotein, for example, under conditions found in lysosomes.
    Type: Grant
    Filed: July 27, 2018
    Date of Patent: October 6, 2020
    Assignee: Genzyme Corporation
    Inventor: James Stefano
  • Amylases, nucleic acids encoding them and methods for making and using them
    Patent number: 10793844
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.
    Type: Grant
    Filed: October 8, 2018
    Date of Patent: October 6, 2020
    Assignee: BASF Enzymes LLC
    Inventors: Kevin A. Gray, Nahla M. Aboushadi, James B. Garrett
  • Polypeptide for hydrolytic cleavage of zearalenone and/or zearalenone derivatives, isolated polynucleotide thereof as well as a polypeptide containing an additive, use of same as well as a process
    Patent number: 10779556
    Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.
    Type: Grant
    Filed: October 16, 2018
    Date of Patent: September 22, 2020
    Assignee: ERBER AKTIENGESELLSCHAFT
    Inventors: Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
  • Methods for making L-glufosinate
    Patent number: 10781465
    Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
    Type: Grant
    Filed: February 27, 2019
    Date of Patent: September 22, 2020
    Assignee: AgriMetis, LLC
    Inventors: Brian Michael Green, Michelle Lorraine Gradley, Annette Alcasabas
  • Non-caloric sweeteners and methods for synthesizing
    Patent number: 10774103
    Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: September 15, 2020
    Assignee: Conagen Inc.
    Inventors: Guohong Mao, Xiaodan Yu
  • Engineered glucosyltransferases
    Patent number: 10774315
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: September 15, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Yougen Li, Ellen D. Semke, Mark S. Payne, Jared B. Parker, Susan Marie Hennessey, Slavko Kralj, Veli Alkan, Richard Bott
  • Transaminases
    Patent number: 10774356
    Abstract: The invention relates to transaminases and their use. The ATAs according to the invention are particularly useful for catalyzing the conversion of amines to ketones and/or vice versa. Preferably, the transaminase (ATA) according to the invention comprises an amino acid sequence with at least 80% homology to SEQ ID NO:1, wherein the amino acid sequence is engineered compared to SEQ ID NO:1 such that it comprises at least a substitution selected from the group consisting of F255L, F255A, F255C, F255D, F255E, F255G, F255H, F255K, F255M, F255N, F255P, F255Q, F255R, F255S, F255T, F255V, F255W, and F255Y.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: September 15, 2020
    Assignee: C-LEcta GmbH
    Inventors: Andreas Vogel, Daniel Schwarze, Rico Czaja, Sally Bayer, Sebastian Bartsch
  • Method for preparing anthocyanin oligomers by using coenzyme derived from sp. strain
    Patent number: 10774351
    Abstract: The present invention relates to a method of preparing an anthocyanin oligomer using a coenzyme derived from an Aspergillus sp. strain, and more particularly to a method of preparing an anthocyanin oligomer by fermenting an anthocyanin monomer with a coenzyme of Aspergillus niger, which is a kind of Aspergillus sp. strain. According to the present invention, in order to overcome contamination problems during the culturing process using Aspergillus niger, a coenzyme of Aspergillus niger is extracted and the fermentation process is performed using the same, whereby an anthocyanin oligomer characterized by reduced concern of contamination and superior radical-scavenging effects, compared to existing anthocyanin monomers, can be produced.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: September 15, 2020
    Assignee: KITTO LIFE
    Inventors: Pyo-Jam Park, Tuk-Rai Jeong, Hyun-Pil Yang, Jin Woo Hwang
  • Nucleic acid-guided nucleases
    Patent number: 10767169
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: September 8, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Compositions and methods for the production and delivery of double stranded RNA
    Patent number: 10757947
    Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.
    Type: Grant
    Filed: August 14, 2018
    Date of Patent: September 1, 2020
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: James A. Baum, Allen T. Christian, Artem Evdokimov, Farhad Moshiri, Lisa Marie Weaver, Haitao Zhang
  • Benzylisoquinoline alkaloid (BIA) precursor producing microbes, and methods of making and using the same
    Patent number: 10752903
    Abstract: Methods and engineered yeast cells for generating a benzylisoquinoline alkaloid product are provided herein. A method comprises providing engineered yeast cells and a feedstock to a reactor. In the reactor, the engineered yeast cells are subjected to fermentation by incubating the engineered yeast cells for a time period to produce a solution comprising the BIA product and cellular material. The solution comprises not more than one class of molecule selected from the group of protoberberine, morphinan, isopavine, aporphine, and benzylisoquinoline. Additionally, at least one separation unit is used to separate the BIA product from the cellular material to provide the product stream comprising the BIA product.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: August 25, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard
  • PQQ-sGDH mutant, polynucleotide and glucose detection biosensor
    Patent number: 10752934
    Abstract: The present invention provides a soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-sGDH) mutant, wherein the amino acid positions thereof correspond to a wild-type PQQ-sGDH sequence of Acinetobacter calcoaceticus as shown in SEQ ID NO 1 comprising one of the following group of mutations: A194F, a combined mutation based on A194F, a combined mutation based on Q192A or a combined mutation based on Q192S. Said PQQ-sGDH mutant has good glucose substrate specificity and significantly reduced cross-reactivity to maltose and the like, and is suitable for detecting glucose in a sample such as blood.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: August 25, 2020
    Assignee: LEADWAY (HK) LIMITED
    Inventors: Zhe Wang, Xin Bi, Riyong Qiu
  • Nucleic acid-guided nucleases
    Patent number: 10745678
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: August 18, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • G proteins
    Patent number: 10738287
    Abstract: The invention provides a mutant of a parent heterotrimeric G protein alpha (G?) subunit, which mutant (i) lacks at least one helix of the helical domain of the parent G? subunit; (ii) is capable of binding to a GPCR in the absence of a heterotrimeric G protein beta (G?) subunit and a heterotrimeric G protein gamma (G?) subunit; and (iii) has an amino acid sequence that contains one or more mutations compared to the amino acid sequence of the parent heterotrimeric G? subunit, which mutations are selected from a deletion, a substitution and an insertion.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: August 11, 2020
    Assignee: Heptares Therapeutics Limited
    Inventors: Byron Carpenter, Andrew Leslie, Rony Nehmé, Christopher Gordon Tate, Antony Warne
  • Methods of producing nor-opioid and nal-opioid benzylisoquinoline alkaloids
    Patent number: 10738335
    Abstract: A method of demethylizing an opioid to a nor-opioid is provided. The method comprises contacting an opioid with at least one enzyme. Contacting the opioid with the at least one enzyme converts the opioid to a nor-opioid. A method of converting a nor-opioid to a nal-opioid is provided. The method comprises contacting a nor-opioid with at least one enzyme. Contacting the nor-opioid with the at least one enzyme converts the nor-opioid to a nal-opioid.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: August 11, 2020
    Assignee: Antheia, Inc.
    Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard