Abstract: The technology relates in part to biological methods for modifying carbon flux in cells, engineered cells and organisms in which cellular carbon flux has been modified, and methods of using engineered cells and organisms for production of organic molecules.
Abstract: In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3? carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
Type:
Grant
Filed:
June 25, 2020
Date of Patent:
November 9, 2021
Assignee:
MANUS BIO INC.
Inventors:
Jason Eric Donald, Ajikumar Parayil Kumaran, Aaron Love, Christopher Toomey, Christine Nicole S. Santos
Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.
Abstract: A method of the bio-production of methionine and/or of its derivatives thereof from a reduced source of sulfur, such as MeSH or MeSNa including genetically modified yeasts, having an increased ability to produce methionine and/or its derivatives thereof, as compared to the parent yeasts.
Type:
Grant
Filed:
July 10, 2018
Date of Patent:
November 2, 2021
Assignee:
ADISSEO FRANCE S.A.S.
Inventors:
Dominique Louis, Karine Jaillardon, Dominique Thomas
Abstract: The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.
Type:
Grant
Filed:
February 12, 2020
Date of Patent:
October 26, 2021
Assignee:
Arizona Board of Regents on behalf of Arizona State University
Inventors:
John Chaput, Andrew Larsen, Matthew Dunn
Abstract: Disclosed herein are CRISPR/Cas9-based gene activation systems that include a fusion protein of a Cas9 protein and a protein having histone acetyltransferase activity, and methods of using said systems.
Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.
Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.
Abstract: The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and a thermostable endoglucanase is present and/or added during liquefaction. The invention also relates to compositions suitable for use in processes of the invention.
Type:
Grant
Filed:
June 7, 2019
Date of Patent:
September 28, 2021
Assignee:
NOVOZYMES A/S
Inventors:
Zhengfang Kang, Christie Strahler, Suzanne Clark, Kristian Bertel Rømer M. Krogh, Tang Lan
Abstract: This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
Type:
Grant
Filed:
January 9, 2020
Date of Patent:
September 28, 2021
Assignee:
Firmenich SA
Inventors:
Laurent Daviet, Letizia Rocci, Michel Schalk
Abstract: The invention discloses a recombinant Corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinant Corynebacterium glutamicum is constructed by overexpressing a protease gene FtsH in a Corynebacterium glutamicum; and the construction method comprises the following steps of: (1) performing PCR on a genome of the Corynebacterium glutamicum, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the Corynebacterium glutamicum, and screening to obtain the recombinant Corynebacterium glutamicum.
Type:
Grant
Filed:
December 10, 2020
Date of Patent:
September 14, 2021
Assignee:
Nanjing Tech University
Inventors:
Hanjie Ying, Dong Liu, Yong Chen, Qingguo Liu, Pingkai Ouyang, Ming Lei, Huanqing Niu, Bin Yu, Di Zhang, Yuhan Yang, Shishi Wu
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C6-C24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The C6-C24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. Also provided are methods of producing unsaturated C6-C24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product alcohols, aldehydes, or acetates.
Type:
Grant
Filed:
May 23, 2019
Date of Patent:
September 7, 2021
Assignee:
Provivi, Inc.
Inventors:
Effendi Leonard, Peter Meinhold, Keith Wampler, Pedro Coelho, Micah Sheppard, Thomas Heel
Abstract: In certain aspects, the disclosure relates to compositions comprising modified Ornithine transcarbamylase (OTC) polyribonucleotides and methods of use.
Abstract: One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof.
Abstract: The present invention relates to synthetic promoters, as well as expression cassettes, and recombinant scaffolds, and methods thereof. In particular embodiments, the synthetic promoter is inducible by one or more chemical compounds present during lignin depolymerization.
Type:
Grant
Filed:
December 11, 2019
Date of Patent:
August 24, 2021
Assignee:
National Technology & Engineering Solutions of Sandia, LLC
Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
Type:
Grant
Filed:
August 11, 2017
Date of Patent:
August 17, 2021
Assignee:
AMYRIS, INC.
Inventors:
Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
Type:
Grant
Filed:
October 30, 2019
Date of Patent:
August 17, 2021
Assignee:
DANISCO US INC
Inventors:
Andrew Shaw, David A Estell, Eugene S Sadlowski, Hiroshi Oh, Louise Wallace, Ronaldus Wilhelmus Joannes Hommes, Sang-Kyu Lee
Abstract: The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
August 24, 2017
Date of Patent:
July 27, 2021
Assignee:
NOVOZYMES A/S
Inventors:
Jens Erik Nielsen, Lars Anderson, Allan Svendsen, Rune Nygaard Monrad, Lars Giger, Vasudeva Prahlada Rao, Sohel Dalal, Santhosh Vasu Mepadam, Marie Thrysoe Kruse, Kasper D. Rand, Pernille F. Jensen