Patents Examined by Gautam Prakash
  • Patent number: 10793817
    Abstract: A cell culture device comprises a first inner cylinder formed in a tubular shape, a cell culture membrane placed to cover over an entire one end of the first inner cylinder and configured to be folded down toward an outer surface of the first inner cylinder along an outer circumference at the one end of the first inner cylinder, and an outer cylinder formed in a tubular shape, configured to place the first inner cylinder inside thereof such that a height direction of the outer cylinder is identical with a height direction of the first inner cylinder, and provided with a first slit in an area overlapping with at least the one end of the first inner cylinder to be extended in the height direction, wherein the cell culture membrane is held between an inner wall surface of the outer cylinder and the outer surface of the first inner cylinder.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: October 6, 2020
    Assignees: TOYODA GOSEI CO., LTD., National University Corporation Yamagata University
    Inventors: Hiroyuki Nakagawa, Seitaro Taki, Hisashi Mizuno, Toshiyuki Hagiyama, Atsuki Yoshimura, Yasuhiko Shinoda, Yasuhiko Ogisu, Masaru Tanaka, Chikako Sato
  • Patent number: 10793890
    Abstract: An apparatus (1000) and kit (1000) for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having (100) a sampling portion (30) and a handling portion (20). The sampling portion (30) is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity (32) that is not substantially defined by space between a plurality of fibers. The sampling device (30) comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 6, 2020
    Inventors: Kenneth R. Davenport, Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
  • Patent number: 10793821
    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: October 6, 2020
    Inventors: Chihiro Uematsu, Muneo Maeshima
  • Patent number: 10787634
    Abstract: Equipment for fermentation of beverages, particularly kombucha, that limits alcohol production during fermentation, increases throughput by reducing fermentation times, and utilizes production plant floorspace efficiently. Unlike traditional fermentation equipment that uses large, often cylindrical, vats, embodiments of the invention use vertical stacks of relatively shallow fermentation trays. The shallow trays increase the ratio of surface area to liquid volume, thereby improving oxygen flow to the fermenting liquid; increased oxygen flow reduces alcohol production for kombucha and reduces fermentation time. Air gaps between trays in the vertical stack, and headspace between the top of the fermenting liquid and the tray top edges, are optimized to promote oxygen flow. Fermentation trays are stacked vertically to maintain high production capacity with limited facility floorspace.
    Type: Grant
    Filed: February 20, 2020
    Date of Patent: September 29, 2020
    Assignee: CLEAR CRAFT, LLC
    Inventors: Toby Tull, David Aycock
  • Patent number: 10781143
    Abstract: The present invention relates to methods and plants for the treatment of an organic waste material, wherein waste is subjected to anaerobic fermentation in a biogas digester; effluent is mechanically separated from the biogas digester into a concentrated fraction and a liquid fraction; the liquid fraction is heated to a high temperature below the boiling point of the liquid; the heated liquid is introduced to a flash column to partially remove volatile carbon dioxide, the pH of the liquid is elevated and ammonia is removed from the liquid.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: September 22, 2020
    Inventors: Birgir Norddahl, Anne Kjaerhuus Nielsen
  • Patent number: 10781417
    Abstract: An apparatus for processing cells is disclosed. In one embodiment, a fixed bed reactor is provided for the cells, the fixed bed reactor including a portion movable from a first position corresponding to a packed condition of the fixed bed to a second position corresponding to a depacked condition of the fixed bed. Movement of the partition facilitates harvesting of the cells there from. Related apparatus, kits, methods, and systems are also disclosed.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: September 22, 2020
    Inventors: Suresh Purushothaman, Jose Antonio Castillo Gonzalez, Jean-Christophe Drugmand, Vishwas Pethe, Derek Pendlebury, Fabien Moncaubeig
  • Patent number: 10760041
    Abstract: The invention discloses a flexible bag for cell cultivation, comprising a cultivation compartment and at least one dialysis compartment, wherein the dialysis compartment(s) is/are delimited from the cultivation compartment by one or more dialysis membranes and is/are fluidically connected to a first and a second port in the bag.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: September 1, 2020
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Karl Liderfelt, Eric Faldt, Andreas Castan
  • Patent number: 10738272
    Abstract: A heating assembly for a bioreactor is disclosed. The heating assembly includes a holder having a plurality of segments coupled to each other to define a unitary structure and a heating component coupled to at least one segment of the plurality of segments. The unitary structure includes a top end, a bottom end, and a side wall. The side wall extends between the top end and the bottom end and along a circumferential direction of the heating assembly to define a cavity. At least a portion of the unitary structure has a gradually varied perimeter along a direction perpendicular to a plane which intersects the side wall and is parallel to the top end and the bottom end.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: August 11, 2020
    Inventors: Ying Mao, Weston Blaine Griffin, Younkoo Jeong
  • Patent number: 10717962
    Abstract: Provided is a method for non-invasively and quantitatively determining multilayerization and differentiation when culturing a cell sheet. Provided is a method for determining a cell state by imaging a cell sheet by using an optical instrument characterized by having a high resolution, and then analyzing the inner structure thereof.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: July 21, 2020
    Inventors: Naoko Senda, Kentaro Osawa
  • Patent number: 10702800
    Abstract: Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: July 7, 2020
    Assignee: Hoffman-La Roche Inc.
    Inventors: Oskar Braunersreuther, Alexander Jockwer, Torsten Klabuhn, Michael Pohlscheidt, Joerg Thiele
  • Patent number: 10688497
    Abstract: The present invention discloses an apparatus for performing PCR by thermal convection. The device includes a first bracket, a second bracket, a temperature sensing device, a power supplying device, a processor and an accommodation space. The device uses transparent conductive film to replace conventional thermostat metal stock to perform a heat process required in the PCR or RT-PCR process. The device further uses a reagent container whose bottom portion contacts the transparent conductive film and being heated by the transparent conductive film to establish a thermal circulation in the reagent container. The device can qualify or quantify the product of PCR or RT-PCR process by further incorporating specific probes, fluorescence material, light source, light receiver and light regulator.
    Type: Grant
    Filed: September 7, 2017
    Date of Patent: June 23, 2020
    Assignee: Credo Diagnostics Biomedical Pte. Ltd.
    Inventors: Yu-Cheng Ou, Cheng-Yueh Chung, Wei-Hao Liu, Han-Yi Chen
  • Patent number: 10669514
    Abstract: Inserts for the wells of tissue culture plates are provided. When placed in a tissue culture well, a bottom surface of a shaft portion of an insert contacts a bottom surface of the well and blocks the growth of cells in that area. The blocked area mimics a “wound” in the layer of cells that grows around it. A cover portion of the insert comprises open channels through which cells, media, etc. can be delivered and gases exchanged while the insert is in place. Once the insert is removed, cell culture with the artificial “wound” is used to test the efficacy of agents with respect to their ability to facilitate cell growth into and closure of the “wound”.
    Type: Grant
    Filed: November 20, 2019
    Date of Patent: June 2, 2020
    Assignee: King Abdulaziz University
    Inventors: Adnan Memic, Tuerdimaimaiti Abudula, Kalamegam Gauthaman
  • Patent number: 10654038
    Abstract: A nucleic acid analysis apparatus with isothermal based amplification includes a chamber, a fluid delivery unit, a thermal unit, a rotational driven unit and at least one optical unit. The chamber includes a cartridge mounted therein. The fluid delivery unit is connected with the chamber and adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction. The thermal unit is disposed in the chamber and adapted to provide a predefined temperature for nucleic acid amplification. The rotational driven unit is connected with the chamber and capable of rotating the cartridge with a predefined program. The at least one optical unit is disposed on the chamber and includes plural optical components for detection.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: May 19, 2020
    Inventors: Qian Liang, Bo Ma, Shuwen An, Weishi Zhang
  • Patent number: 10626360
    Abstract: A nucleic acid amplification device which includes a flow channel for feeding a reaction solution containing a target nucleic acid includes: a nucleic acid amplification reaction portion that amplifies the target nucleic acid contained in the reaction solution; and a fed-solution retention portion that retains the reaction solution. The flow channel includes a first flow channel disposed in the nucleic acid amplification reaction portion and a second flow channel disposed in the fed-solution retention portion, and the reaction solution fed from the first flow channel is retained in the second flow channel.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: April 21, 2020
    Inventors: Hiroaki Tachibana, Shogo Shibuya
  • Patent number: 10617116
    Abstract: The present invention provides a cryopreservation device that makes it possible to automatically extract an intended sample, and prevent the temperature of samples other than the intended sample from increasing. A cryopreservation device is provided, including: a cryopreservation vessel that internally accommodates a drawer configured to store vials side by side in a vertical direction in each box and has an opening communicating with a task space provided in an upper surface thereof; a drawer-raising/lowering device that raises and lowers the drawer in the vertical direction via the opening in a state where the drawer is gripped and maintains the drawer at an arbitrary height; a first stage that is provided above the cryopreservation vessel so as to be adjacent to the opening as seen in plan view and allows the box to be placed thereon; and a second pressing device that presses the box to move the box to the first stage.
    Type: Grant
    Filed: March 17, 2016
    Date of Patent: April 14, 2020
    Inventors: Hiroki Aota, Shigehiro Yoshimura, Kouji Makino
  • Patent number: 10613015
    Abstract: A particle analysis system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet; and a particle analyzer configured to measure a size and a position of particles in the microchannel. A particle sorting system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; and a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: April 7, 2020
    Inventors: Dino Di Carlo, Soojung Hur
  • Patent number: 10544453
    Abstract: There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells 7A are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each well 7A. A fluorescence measurement value [DNA]raw obtained from the well 7A and a fluorescence measurement value [DNA]bg obtained from a connection wall near the well 7A are detected, and the fluorescence measurement value [DNA]bg is subtracted from the fluorescence measurement value [DNA]raw to determine fluorescence strength [DNA]real of the well 7A.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: January 28, 2020
    Inventors: Yuichi Tamaoki, Akifumi Iwama, Yasuaki Sonoda
  • Patent number: 10544387
    Abstract: Aspects of the disclosure relate to bioreactors for maintaining biological objects (e.g., organ or engineered tissues) under culture conditions. In some embodiments, bioreactors and cover assemblies are provided that comprise a support base for supporting a biological object. In some embodiments, bioreactors and cover assemblies are provided that comprise a movable support base configured such that a user can manipulate its position and thus the relative position of the biological object.
    Type: Grant
    Filed: March 27, 2014
    Date of Patent: January 28, 2020
    Assignee: Biostage, Inc.
    Inventor: Herbert Hedberg
  • Patent number: 10533149
    Abstract: A tempering device for tempering of biomass contained in a container, including at least one tempering mechanism, and at least one conveying device for conveying a mass flow of biomass in a main flow direction. The at least one conveying device includes at least one agitator, and wherein the at least one tempering mechanism is arranged in the main flow direction generated by the at least one conveying device. The at least one tempering mechanism is tubular with an inner tube and with an outer tube arranged coaxially to the inner tube. The inner tube and the outer tube form an intermediate space through which a tempering medium may flow. The tempering mechanism is oriented relative to the conveying device such that, in operation of the heating device, at least 40% of the delivery volume of the mass flow generated by the conveying device flows through the inner tube.
    Type: Grant
    Filed: October 22, 2015
    Date of Patent: January 14, 2020
    Inventors: Wilfried Stiller, Ulrich Lüdersen, Eilert Balssen
  • Patent number: 10519414
    Abstract: Disclosed is a method of continuously culturing cells, including culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells, detaching the adhered cells when the density of the cells cultured in the compartment of the culture vessel is a reference value or more, and obtaining the cells detached from the culture vessel, which is maintained in a sealed state.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: December 31, 2019
    Assignee: MEDIKAN INC
    Inventor: Hee Young Lee