Abstract: A nucleic acid analysis apparatus with isothermal based amplification includes a chamber, a fluid delivery unit, a thermal unit, a rotational driven unit and at least one optical unit. The chamber includes a cartridge mounted therein. The fluid delivery unit is connected with the chamber and adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction. The thermal unit is disposed in the chamber and adapted to provide a predefined temperature for nucleic acid amplification. The rotational driven unit is connected with the chamber and capable of rotating the cartridge with a predefined program. The at least one optical unit is disposed on the chamber and includes plural optical components for detection.

Abstract: A nucleic acid amplification device which includes a flow channel for feeding a reaction solution containing a target nucleic acid includes: a nucleic acid amplification reaction portion that amplifies the target nucleic acid contained in the reaction solution; and a fed-solution retention portion that retains the reaction solution. The flow channel includes a first flow channel disposed in the nucleic acid amplification reaction portion and a second flow channel disposed in the fed-solution retention portion, and the reaction solution fed from the first flow channel is retained in the second flow channel.

Abstract: The present invention provides a cryopreservation device that makes it possible to automatically extract an intended sample, and prevent the temperature of samples other than the intended sample from increasing. A cryopreservation device is provided, including: a cryopreservation vessel that internally accommodates a drawer configured to store vials side by side in a vertical direction in each box and has an opening communicating with a task space provided in an upper surface thereof; a drawer-raising/lowering device that raises and lowers the drawer in the vertical direction via the opening in a state where the drawer is gripped and maintains the drawer at an arbitrary height; a first stage that is provided above the cryopreservation vessel so as to be adjacent to the opening as seen in plan view and allows the box to be placed thereon; and a second pressing device that presses the box to move the box to the first stage.

Abstract: A particle analysis system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet; and a particle analyzer configured to measure a size and a position of particles in the microchannel. A particle sorting system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; and a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet.

Abstract: There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells 7A are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each well 7A. A fluorescence measurement value [DNA]raw obtained from the well 7A and a fluorescence measurement value [DNA]bg obtained from a connection wall near the well 7A are detected, and the fluorescence measurement value [DNA]bg is subtracted from the fluorescence measurement value [DNA]raw to determine fluorescence strength [DNA]real of the well 7A.

Abstract: Aspects of the disclosure relate to bioreactors for maintaining biological objects (e.g., organ or engineered tissues) under culture conditions. In some embodiments, bioreactors and cover assemblies are provided that comprise a support base for supporting a biological object. In some embodiments, bioreactors and cover assemblies are provided that comprise a movable support base configured such that a user can manipulate its position and thus the relative position of the biological object.

Abstract: A tempering device for tempering of biomass contained in a container, including at least one tempering mechanism, and at least one conveying device for conveying a mass flow of biomass in a main flow direction. The at least one conveying device includes at least one agitator, and wherein the at least one tempering mechanism is arranged in the main flow direction generated by the at least one conveying device. The at least one tempering mechanism is tubular with an inner tube and with an outer tube arranged coaxially to the inner tube. The inner tube and the outer tube form an intermediate space through which a tempering medium may flow. The tempering mechanism is oriented relative to the conveying device such that, in operation of the heating device, at least 40% of the delivery volume of the mass flow generated by the conveying device flows through the inner tube.

Abstract: Disclosed is a method of continuously culturing cells, including culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells, detaching the adhered cells when the density of the cells cultured in the compartment of the culture vessel is a reference value or more, and obtaining the cells detached from the culture vessel, which is maintained in a sealed state.

Abstract: Height adjusting device (1) for adjusting the height of an agitating device (2) in a digester (101) of a biogas plant (100), biogas plant (100) comprising a digester (101), a support unit (10), a displacement member (6), an agitating device (2), and a height adjusting device, and method for operating an agitating device (2). The height adjusting device comprises a housing unit (4), a drum device (5) rotatably accommodated on the housing unit (4), and a displacement member (6). The displacement member (6) is in functional connection with the drum device (5) and the agitating device (2) and the displacement member (6), depending on the direction of rotation of the drum device (5), can be wound onto the drum device (5) or unwound from the drum device (5) in sections, so that the agitating device (2) can be displaced upwardly or downwardly.

Abstract: A method of enhancing plant growth comprises a) selecting a mobile, self-contained system comprising a mobile structure, a vessel supported by the structure for receiving water and nutrients, a recirculating pump, a generator powering the pump, and a discharge line adapted for discharging contents of the vessel directly into an irrigation system; b) introducing into the vessel water and nutrient; c) moving the system; d) after moving, coupling the discharge line to an irrigation system or spray; and e) pumping the water and nutrient in the vessel with the pump out of the vessel through the discharge line directly into the irrigation system or spray.

Abstract: The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed.

Abstract: A compost retention unit with removable partition is provided. The compost retention unit includes mesh retention material secured to interconnected frame structures such that the mesh retention material and the plurality of frame structures can cooperate to retain compost within the unit when the unit is fully assembled upon a generally flat surface. The removable partition can be secured to provide separate enclosures, and can be removed to facilitate transfer or turning of the compost. Preferably, the compost retention unit can be collapsed for storage or shipment and expanded to assemble the retention unit for use. Preferably the compost retention unit includes three rectangular frame structures which pivot to assemble the compost retention unit for use. Most preferably, the frame structures are made of four lengths of wooden “two-by-four” or “two-by-six” framing materials, and the mesh retention material is preferably a wire mesh material.

Abstract: Provided is a cell suction system that supports suction work of a substance within a cell. The cell suction system includes: a suction section including a tubular tip configured to suction the substance from the inside of the cell received in a container; a detection section configured to acquire information on a front end part of the tip; and a conveyance section configured to make the suction section three-dimensionally movable, the conveyance section being configured to move the suction section to guide the front end part of the tip attached to the suction section into one specific cell based on the information obtained in the detection section.

Abstract: The present invention relates to a device for bioassisted conversion of carbon dioxide to organic compounds that can be used a fuels and chemicals. The present invention also relates to a bioassisted process of converting carbon dioxide to organic compounds.

Abstract: An apparatus is provided for use with a fluid that contains biological cells, e.g. seminal fluid. The apparatus (20) includes a tray (22) shaped to define a flat upper surface configured to support the fluid, and an underside. The apparatus (20) further includes a plurality of protrusions protruding from the underside of the tray, which may serve to separate the underside from a flat surface on which the tray is placed, or as delineating protrusions that facilitate subsequent retrieval of the fluid following placement on the tray. The tray has a thickness between 0.4 and 0.8 mm, and is transparent or translucent. The upper surface preferably has a level of hydrophobicity that is sufficient for droplets to maintain their shape to a certain extent. A handle (52) facilitates handling of the tray. The apparatus typically fits into a standard-sized cryogenic vial (70).

Abstract: Methods, apparatus, and system for a compost system to reduce operator time, reduce power utilization, increase intensity of land use by the compost system, increase efficiency of biological activity in bulk material composted by the compost system, reduce odor production by the compost system, and to reuse water applied to and which may leach out of bulk material. The compost system may comprise an aerated pad, a pivot arm attached to a center of the aerated pad, and a control module. The aerated pad may receive a bulk material to be composted into a compost product. The aerated pad may comprise an air nozzle and a drain to direct water from the bulk material to a water collection system. The pivot arm may deposit the bulk material on the aerated pad. The control module may manage a temperature inside the bulk material using the air nozzle.

Abstract: This disclosure is directed to methods and systems for growing and/or harvesting cells. The method can include seeding cells on a plurality of carriers. The method can also include incubating the carriers under conditions suitable for cell growth.

Abstract: A method of producing cellulose liquefaction and a cellulose liquefaction module that mixes and circulates cellulose material as a solid, and provides evaporative cooling in the module. The module includes a container having a vertically disposed tube that houses an auger conveyor that mixes and circulates solid cellulose material. Steam ports and activation agent injection ports are disposed within the tube to liquify the cellulose material. A forced air blower pushes air through the liquified cellulose material to provide evaporative cooling before discharge from the container.

Abstract: The apparatus for inoculating agar plates includes a spray chamber having an upper opening for receiving an atomized microbial suspension and a lower opening for receiving an agar plate. The apparatus also includes an atomizer including a reservoir and a fluid tube for delivering the microbial suspension to the atomizer nozzle. A containment feature extends around an inner surface of the spray chamber to catch any drop that may form on its inner wall and advance down towards the lower opening. The spray chamber allows multiple agar plates to be quickly inoculated without cross-contamination of agar habitats, without contaminating the outside of the plates, and without contaminating the work area.

Abstract: An inner bag of a cell culture package is enclosed in an outer bag such that a rubber stopper is exposed to the outside. A closed space surrounded by the outer surface of the inner bag and the inner surface of the outer bag is sterile. This allows the outer surface of the inner bag, which is used for containing cell-containing deformable materials, to be maintained in a sterile condition. Since the cell culture package can be sterilized before a cell-containing deformable material is placed therein, the sterilization conditions can be relatively easily determined without considering the influence on the cell-containing deformable material. In addition, a holder allows liquid to pass through the bottom surface and side surface of a recess and the bottom surface and the side surface of a cover.