Patents Examined by Iqbal H Chowdhury
  • Patent number: 10501727
    Abstract: This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: December 10, 2019
    Inventors: Luet Lok Wong, Stephen Graham Bell, Christopher Whitehouse
  • Patent number: 10501731
    Abstract: The specification discloses Clostridial toxins or Clostridial toxin chimeras comprising an inactivation cleavage site, polynucleotide molecules encoding such toxins or chimeras, compositions comprising such toxins or chimeras, and method of producing such toxins or chimeras.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: December 10, 2019
    Assignee: Allergan, Inc.
    Inventors: Lance E. Steward, Sanjiv Ghanshani, Ester Fernandez-Salas, Marcella A. Gilmore, Joseph Francis, Kei Roger Aoki
  • Patent number: 10494653
    Abstract: The invention is directed to a method for recovering a lipid or hydrocarbon from a fermentation mixture, comprising the steps of—providing a fermentation mixture wherein the lipid or hydrocarbon is produced by microbial fermentation in a fermentation vessel, which mixture comprises an aqueous phase and a liquid product phase, wherein the liquid product phase comprises the lipid or hydrocarbon; and—feeding at least part of the aqueous phase and part of the liquid product phase to a second vessel, thereby forming a second mixture; and—promoting phase-separation of the aqueous and product phase by injecting a gas into the second mixture, thereby separating the product phase from the aqueous phase; and—collecting the product phase comprising the lipid or hydrocarbon.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: December 3, 2019
    Inventors: Maria Claudia Cuellar Soares, Lucas Antonius Maria van der Wielen, Arjan Sebastiaan Heeres
  • Patent number: 10494686
    Abstract: The present invention relates to a composition for epimerization of a non-phosphorylated hexose, comprising sugar epimerases derived from thermophiles, and a method for preparing a non-phosphorylated hexose epimer using the composition. The sugar epimerases derived from thermophiles according to the present invention can effectively catalyze an epimerization reaction of a non-phosphorylated hexose and can easily produce an epimer form of the non-phosphorylated hexose, in particular a rare sugar hexose, and thus can be usefully utilized in the pharmaceutical and food industry.
    Type: Grant
    Filed: January 30, 2018
    Date of Patent: December 3, 2019
    Assignee: CJ CheilJedang Corporation
    Inventors: Dong Woo Lee, Sun Mi Shin, Yong Jik Lee, Sang Jae Lee
  • Patent number: 10487342
    Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: November 26, 2019
    Assignee: Genomatica, Inc.
    Inventors: Priti Pharkya, Anthony P. Burgard, Mark J. Burk
  • Patent number: 10472656
    Abstract: A fermentation process for fermenting a starch hydrolysate containing 60-98 weight percent of glucose based on total carbohydrate and 0.3-5% weight percent of isomaltose based on total carbohydrate to a non-ethanol fermentation product is carried out. The method comprises: a) forming a fermentation broth containing the starch hydrolysate and a genetically modified microorganism containing i) an exogenous gene encoding transporter capable of transporting isomaltose ii) an exogenous gene encoding an enzyme capable of converting isomaltose to glucose; and b) fermenting the starch hydrolysate in the fermentation broth to produce a non-ethanol fermentation product. This fermentation process may be carried out as a single step fermentation or a batch process. The genetically modified microorganism may contain an exogenous gene encoding an enzyme that catalyzes the formation of a product other than ethanol.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: November 12, 2019
    Inventors: Keith M. Brady, Christopher K. Miller
  • Patent number: 10428359
    Abstract: A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a C4-dicarboxylic acid-uptake carrier such as DctA, DcuA, and DcuB is increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: October 1, 2019
    Assignee: AJINOMOTO CO, INC.
    Inventors: Mika Moriya, Kazuyuki Hayashi, Akari Yokokawa
  • Patent number: 10400201
    Abstract: A method is provided for treating distiller's grains with solubles (DGS) to produce one or more byproducts. The method includes separating the DGS into a low protein mixture and a high protein mixture. The method includes generating, from the low protein mixture, a biogas by an anaerobic digestion process. The method includes generating, from the high protein mixture, at least one of a vegetable oil from a vegetable oil separation process, a high protein animal feed from a separation process and a microalgae biomass material from a microalgae production process.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: September 3, 2019
    Inventor: Guo Yu
  • Patent number: 10392606
    Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.
    Type: Grant
    Filed: December 10, 2017
    Date of Patent: August 27, 2019
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Basil J. Nikolau, Marna Yandeau-Nelson, Fuyuan Jing
  • Patent number: 10385363
    Abstract: The present invention relates to a method of producing drimenol and/or drimenol derivatives by contacting at least one polypeptide with farnesyl diphosphate. The method may be performed in vitro or in vivo. The present invention also provides amino acid sequences of polypeptides useful in the method of the invention and nucleic acid encoding the polypeptides of the invention. The method further provides host cells or organisms genetically modified to express the polypeptides of the invention and useful to produce drimenol and/or drimenol derivatives.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: August 20, 2019
    Assignee: Firmenich SA
    Inventors: Yu-Hua Zhang, Michel Schalk, Olivier Haefliger, Xiu-Feng He, Fabienne Deguerry
  • Patent number: 10385365
    Abstract: The present technology provide a method of dewatering whole stillage. The addition of a xylanase in combination with a pectinase results in a wet cake with a higher dry mass. The advantage here is less energy consumption while drying.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: August 20, 2019
    Assignees: DIREVO Industrial Biotechnology GmbH, BASF Enzymes, LLC
    Inventors: Marco Kraemer, Vitaly Svetlichny
  • Patent number: 10364328
    Abstract: The present invention relates to a method for pretreating lignocellulose by using an acid-base mixture catalyst. The method pretreats lignocellulose, by using a mixture catalyst of an acid and a base, so as not to pass through additional neutralization steps, and carries out pretreatment and simultaneous saccharification and fermentation through an identical single reactor process, thereby having an effect of producing ethanol in an excellent production yield from lignocellulosic biomass while simplifying the total process and reducing equipment costs and total processing costs.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: July 30, 2019
    Assignee: Korea University Research and Business Foundation
    Inventors: Kyoung Heon Kim, Young Hoon Jung
  • Patent number: 10316307
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: June 11, 2019
    Inventors: Marc D. Morant, Mikako Sasa, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 10287565
    Abstract: Provided are a novel isoprene synthase derived from sweet potato and a method of preparing isoprene using the same, and more specifically, a novel isoprene synthase derived from sweet potato, a gene encoding the isoprene synthase, a host cell transformed with the gene, and a method of preparing isoprene using the same. The isoprene synthase of the present invention may have higher isoprene productivity as compared to isoprene synthases known in the related art to thereby be effectively used in isoprene biosynthesis and preparation of an isoprene polymer using the same.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: May 14, 2019
    Assignee: SK Innovation Co., Ltd.
    Inventors: Merja Oja, Anne Huuskonen, Marja Iimen, Laura Ruohonen, Outi Koivistoinen, Simon Moon Geun Jung, Jae Hoon Jo, Sang Min Lee
  • Patent number: 10280440
    Abstract: [Problems] To provide a method of producing a lipid, containing enhancing productivity of medium chain fatty acids or the lipid containing these medium chain fatty acids as components. [Means to solve] A method of producing a lipid, containing the steps of: culturing a transformant in which a gene encoding any one of the following proteins (A) to (C) is introduced into a host, and collecting a lipid from the cultured product: (A) a protein consisting of the amino acid sequence of the 91st to 348th positions set forth in SEQ ID NO: 1; (B) a protein consisting of an amino acid sequence having 80% or more identity with the amino acid sequence of the 91st to 348th positions set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; and (C) a protein containing the amino acid sequence of the protein (A) or (B), and having acyl-ACP thioesterase activity.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: May 7, 2019
    Assignee: Kao Corporation
    Inventor: Tatsuro Ozaki
  • Patent number: 10280407
    Abstract: The present invention concerns a recombinant strain belonging to the order of Actinomycetales, wherein at least one gene encoding an enzyme having vanillin reductase activity is non-functional. The present invention is also related to a process for producing vanillin or a precursor thereof, comprising the culture of a recombinant strain in an appropriate medium comprising a substrate, and recovery of the produced vanillin or precursor thereof.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: May 7, 2019
    Assignee: Rhodia Operations
    Inventors: Mirjana Gelo-Pujic, Antoine Amory
  • Patent number: 10266856
    Abstract: A method for preparing a fermentation product including lactic acid, the method including: a) treating lignocellulosic material with caustic magnesium salt in the presence of water to provide treated aqueous lignocellulosic material; b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material comprising fermentable carbohydrate and a solid lignocellulosic fraction; c) simultaneously with step b), fermenting the saccharified aqueous lignocellulosic material in the presence of both a lactic acid forming microorganism and caustic magnesium salt to provide an aqueous fermentation broth comprising magnesium lactate and a solid lignocellulosic fraction; d) recovering magnesium lactate from the broth, wherein the saccharification and the fermentation are carried out simultaneously.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: April 23, 2019
    Assignee: PURAC BIOCHEM BV
    Inventors: Peter Johannes Marie Baets, David Sanchez Garcia, Willem Jacob Groot, André Banier De Haan
  • Patent number: 10260059
    Abstract: The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: April 16, 2019
    Assignees: Zhejiang University of Technology, Zhejiang Chiral Medicine Chemicals Co. Ltd.
    Inventors: Renchao Zheng, Yuguo Zheng, Qin Zhang, Youming Huang, Jianfeng Weng, Tianchun Liu, Weirong Fan
  • Patent number: 10253285
    Abstract: The present invention a method for simultaneously reducing urethane and its precursors levels during Chinese liquor production process, which relates to the field of fermented food production. The present invention is carried out through inoculating Bacillus amyloliquefaciens JP21 or combines with urease into the grains for fermentation. The content of EC and urea in the fermented grains after 5-day fermentation could reduce 30.16% and 50.05% respectively, With an addition of the B. amyloliquefaciens during grains fermentation, EC and urea levels are successfully controlled, and at the same time aroma and tastes of Chinese liquor hasn't been affected.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: April 9, 2019
    Inventors: Fang Fang, Xia Ding, Qiaoyu Li, Fan Liu, Xinhu Zhou, Xiang Chen, Jian Chen, Guocheng Du
  • Patent number: 10253343
    Abstract: A method of producing a sugar liquid and a xylooligosaccharide includes Steps (1) to (3): Step (1): hydrolyzing a cellulose-containing biomass with a filamentous fungus-derived cellulase; Step (2): subjecting the hydrolysate of Step (1) to solid-liquid separation, and filtering the solution component through an ultrafiltration membrane to recover cellulase as a non-permeate, and to recover a sugar liquid as a permeate; and Step (3): reacting the recovered cellulase in Step (2) with a xylan-containing material, and recovering a xylooligosaccharide produced, Step (3) being independent from Step (1).
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: April 9, 2019
    Assignee: Toray Industries, Inc.
    Inventors: Chiaki Yamada, Hiroyuki Kurihara, Katsushige Yamada