Patents Examined by Iqbal H Chowdhury
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Patent number: 11884923Abstract: Provided are a fructose-4-epimerase variant having tagatose conversion activity, and a method of preparing tagatose using the same.Type: GrantFiled: September 27, 2019Date of Patent: January 30, 2024Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Mi Lee, Eul-Soo Park, Il Hyang Park, Sun Mi Shin, Sung Jae Yang, Ran Young Yoon, Eun Jung Choi, Seong Bo Kim, Seung Won Park
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Patent number: 11866737Abstract: The invention relates to a 2-isopropyl malate synthase, a genetically engineered bacterium for producing L-leucine and application thereof and belongs to the field of metabolic engineering. The genetically engineered bacterium is obtained by overexpressing an isopropyl malate synthase coding gene leuAM for relieving feedback inhibition by L-leucine, an acetohydroxy acid synthase coding gene ilvBNM for relieving feedback inhibition by L-isoleucine, a 3-isopropyl malate dehydrogenase coding gene leuB and a 3-isopropyl malate dehydratase coding gene leuCD in host cells. The genetically engineered bacterium for producing the L-leucine is free from nutritional deficiency, rapid in growth, short in fermentation period, high in yield and high in conversion rate.Type: GrantFiled: December 29, 2020Date of Patent: January 9, 2024Assignee: Tianjin University of Science and TechnologyInventors: Chenglin Zhang, Qingyang Xu, Yanjun Li, Yu Zhang, Yingzi Li, Fuzhou Zhu, Nan Lu, Shibao Han, Xierong Dong, Zishen Wang, Hao Xu, Ziyi Li
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Patent number: 11851662Abstract: Disclosed herein are methods and compositions for generation of cell lines to promote unnatural amino acid-containing protein production using genome engineering technology.Type: GrantFiled: June 2, 2018Date of Patent: December 26, 2023Assignee: Ambrx, Inc.Inventors: Sigeng Chen, Yingchun Lu, Feng Tian
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Patent number: 11851691Abstract: The present disclosure provides a fusion protein comprising a fucosidase or a truncated fragment or a mutant thereof fuses with either N-terminal end or C-terminal end of the endoglycosidase or a truncated fragment of mutant thereof. The present disclosure also provides a nucleic acid molecule expressing the fusion protein and a method for remodeling a glycan of an antibody Fc region.Type: GrantFiled: August 5, 2020Date of Patent: December 26, 2023Assignee: CHO PHARMA INC.Inventors: Kuo-Ching Chu, Lin-Ya Huang, Yi-Fang Zeng
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Patent number: 11845923Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.Type: GrantFiled: July 7, 2021Date of Patent: December 19, 2023Assignee: Singular Genomics Systems, Inc.Inventors: Souad Naji, Carl W. Fuller, Eli Glezer, Andrew Spaventa
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Patent number: 11834692Abstract: Disclosed are components and methods for preparing tagatose from galactose via isomerization reactions using engineered components. The engineered components include microbial cells and methods for preparing microbial cells that have been engineered to catalyze isomerization of galactose to tagatose, in which the microbial cells express cytoplasmically an exogenous L-arabinose isomerase enzyme. The disclosed microbial cells may further be modified for use in methods for preparing tagatose from galactose via isomerization reactions where the microbial cells are treated with reagents that permeabilize the cells. The disclosed methods enable isomerization reactions of galactose to tagatose at relatively high rates, high conversions, and elevated temperatures.Type: GrantFiled: February 5, 2021Date of Patent: December 5, 2023Assignee: Trustees of Tufts CollegeInventors: Nikhil U. Nair, Josef R. Bober
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Patent number: 11834690Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: November 16, 2021Date of Patent: December 5, 2023Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 11827900Abstract: Described herein are engineered nucleases comprising mutations in the cleavage domain (e.g., FokI or homologue thereof) and/or DNA binding domain (zinc finger protein, TALE, single guide RNA) such that on-target specificity is increased.Type: GrantFiled: July 14, 2020Date of Patent: November 28, 2023Assignee: Sangamo Therapeutics, Inc.Inventors: Jeffrey C. Miller, Edward J. Rebar
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Patent number: 11827692Abstract: The invention provides a method for culturing mammalian cells. The method provides greater control over cell o growth to achieve high product titer cell cultures.Type: GrantFiled: March 8, 2023Date of Patent: November 28, 2023Assignee: AMGEN INC.Inventors: Brian D. Follstad, Rebecca E. McCoy, Arvia E. Morris
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Patent number: 11802300Abstract: A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3?-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.Type: GrantFiled: October 30, 2019Date of Patent: October 31, 2023Assignee: TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGYInventors: Xixian Xie, Heyun Wu, Jiachu Wang, Faqing Wu, Xiaoqian Liu, Yanan Hao
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Patent number: 11788103Abstract: The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.Type: GrantFiled: July 24, 2020Date of Patent: October 17, 2023Assignee: LanzaTech, Inc.Inventors: Sean Dennis Simpson, Allan Haiming Gao, Robert John Conrado, James Daniel Winkler, Alexander Paul Mueller, Steven Brown, Loan Phuong Tran, Michael Koepke, Christophe Daniel Mihalcea
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Patent number: 11781165Abstract: Sedoheptulose, which is a saccharide falling within the categories of ketoses and heptuloses, is one of a small number of heptuloses occurring in nature. A method for producing sedoheptulose may use a bacterium, and/or may improve the productivity of sedoheptulose by the bacterium, and the bacterium. To solve this problem, provided are a method for producing sedoheptulose using a bacterium owing to the deletion or attenuation of a specific enzymatic function, a method for improving the productivity of sedoheptulose by the bacterium, and the bacterium.Type: GrantFiled: April 26, 2019Date of Patent: October 10, 2023Assignee: NAGASE & CO., LTD.Inventor: Shogo Yamamoto
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Patent number: 11781125Abstract: Compositions and methods are provided for variant Cas systems and elements comprising such systems, including, but not limiting to, Cas endonuclease variants, guide polynucleotide/Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.Type: GrantFiled: December 11, 2018Date of Patent: October 10, 2023Assignee: DANISCO US INC.Inventors: Ryan L. Frisch, Hongxian He
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Patent number: 11773382Abstract: The present invention provides several non-naturally occurring sulfotransferase enzymes that have been engineered to react with aryl sulfate compounds as sulfo group donors, instead of the natural substrate 3?-phosphoadenosine 5?-phosphosulfate (PAPS), and with heparosan-based polysaccharides, particularly heparan sulfate, as sulfo group acceptors. Each of the engineered sulfotransferase enzymes have a biological activity characterized by the position within the heparosan-based polysaccharide that receives the sulfo group, including glucosaminyl N-sulfotransferase activity, hexuronyl 2-O sulfotransferase activity, glucosaminyl 6-O sulfotransferase activity, or glucosaminyl 3-O sulfotransferase activity. Methods of using the engineered sulfotransferases to produce sulfated heparosan-based polysaccharides, including polysaccharides having anticoagulant activity, are also provided.Type: GrantFiled: August 24, 2022Date of Patent: October 3, 2023Assignee: OPTIMVIA, LLCInventors: Tarsis Gesteira Ferreira, Daniel H. Lajiness
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Patent number: 11767518Abstract: The present invention provides several non-naturally occurring sulfotransferase enzymes that have been engineered to react with aryl sulfate compounds as sulfo group donors, instead of the natural substrate 3?-phosphoadenosine 5?-phosphosulfate (PAPS), and with heparosan-based polysaccharides, particularly heparan sulfate, as sulfo group acceptors. Each of the engineered sulfotransferase enzymes have a biological activity characterized by the position within the heparosan-based polysaccharide that receives the sulfo group, including glucosaminyl N-sulfotransferase activity, hexuronyl 2-O sulfotransferase activity, glucosaminyl 6-O sulfotransferase activity, or glucosaminyl 3-O sulfotransferase activity. Methods of using the engineered sulfotransferases to produce sulfated heparosan-based polysaccharides, including polysaccharides having anticoagulant activity, are also provided.Type: GrantFiled: August 24, 2022Date of Patent: September 26, 2023Assignee: OPTIMVIA, LLCInventors: Tarsis Gesteira Ferreira, Daniel H. Lajiness
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Patent number: 11767342Abstract: Herein is reported a method for the enzymatic production of an antibody with a modified glycosylation in the Fc-region comprising the steps of incubating the antibody light chain affinity ligand-bound monoclonal antibody with a glycosylation in the Fc-region with a first enzyme for a time sufficient and under conditions suitable to modify the glycosylation of the Fc-region, recovering the antibody from the antibody light chain affinity ligand, incubating the recovered antibody in solution with a second enzyme for a time sufficient and under conditions suitable to modify the glycosylation of the Fc-region to a defined form, separating the antibody with the modified glycosylation in the Fc-region from the second enzyme in a cation exchange chromatography, and thereby producing the antibody with a modified glycosylation in the Fc-region.Type: GrantFiled: June 20, 2019Date of Patent: September 26, 2023Assignee: Hoffmann-La Roche Inc.Inventors: Thomas Dams, Roberto Falkenstein, Sebastian Malik, Ingrid Grunert, Marco Thomann, Matthias Freiherr Von Roman, Heiko Walch
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Patent number: 11753615Abstract: The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of Saccharomyces cerevisiae, method for constructing the same, and its use for brewing, the genetically engineered strain of Saccharomyces cerevisiae heterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. The Saccharomyces cerevisiae strain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.Type: GrantFiled: August 25, 2021Date of Patent: September 12, 2023Assignee: Tianjin University of Science and TechnologyInventors: Yefu Chen, Wenqi Shi, Guo Zhang, Ruirui Li, Yanfang Chen, Huan Wang, Chunhong Sun, Dongguang Xiao, Xiaole Wu, Xuewu Guo
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Patent number: 11753646Abstract: Provided are a tagatose-bisphosphate aldolase variant having tagatose conversion activity, and a method of preparing tagatose using the same.Type: GrantFiled: September 27, 2019Date of Patent: September 12, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Mi Lee, Eul-Soo Park, Il Hyang Park, Sun Mi Shin, Sung Jae Yang, Ran Young Yoon, Eun Jung Choi, Seong Bo Kim, Seung Won Park
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Patent number: 11746351Abstract: A genetically engineered microorganism for the production of a cannabinoid biosynthetic pathway product is described. The genetically engineered microorganism comprises at least one nucleic acid molecule encoding at least one cannabinoid biosynthetic pathway enzyme. The disclosure also relates to methods for producing a cannabinoid biosynthetic pathway product using a genetically engineered microorganism.Type: GrantFiled: April 29, 2019Date of Patent: September 5, 2023Assignee: Algae-C Inc.Inventors: William Mather Almon Carscallen, Isabel Desgagné-Penix, Jean-Francois Lemay
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Patent number: 11746130Abstract: The present disclosure relates to a novel protein variant having an activity of exporting 5?-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5?-inosine monophosphate using the microorganism.Type: GrantFiled: June 6, 2022Date of Patent: September 5, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Jin Ah Rho, Byoung Hoon Yoon, So-jung Park, Min Ji Baek, Ji Hye Lee