Patents Examined by Jezia Riley
  • Patent number: 10577396
    Abstract: An object of the present invention is to conduct a search for a compound to be arranged at a terminal of a peptide nucleic acid effective in detecting a single nucleotide polymorphism. Provided is a tolan compound represented by the following formula (1): wherein R1 represents a phenyl group or a naphthyl group, the phenyl group may have 1 to 5 substituents that are identical to or different from each other, and the naphthyl group may have 1 to 7 substituents that are identical to or different from each other.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: March 3, 2020
    Assignee: Osaka University
    Inventors: Kunihiro Kaihatsu, Nobuo Kato, Kenji Takagi
  • Patent number: 10577646
    Abstract: Provided herein is technology relating to the manipulation and detection of nucleic acids, including but not limited to compositions, methods, and kits related to nucleotides comprising a chemically reactive linking moiety.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: March 3, 2020
    Assignee: ABBOTT MOLECULAR INC.
    Inventor: Dae Hyun Kim
  • Patent number: 10577652
    Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: March 3, 2020
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
  • Patent number: 10570463
    Abstract: In some aspects, the disclosure provides compounds comprising nucleic acid modifying moieties, such as nucleic acid binding dyes comprising activatable groups. In some aspects, the disclosure provides nucleic acid probes comprising compounds of the disclosure, and methods of making the same. In some aspects, the disclosure provides methods of using compounds of the disclosure, such as methods of labeling and/or detecting non-viable organisms or non-viable cells, and methods of detecting contamination or infection.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: February 25, 2020
    Assignee: BIOTIUM, INC.
    Inventors: Fei Mao, Patrick Gordon McGarraugh, Alexis Spain Madrid, Wai-Yee Leung, Lori M. Roberts
  • Patent number: 10570446
    Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: February 25, 2020
    Assignee: THE TRUSTEE OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
  • Patent number: 10570445
    Abstract: FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: February 25, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Xiangxu Kong, Gene Shen
  • Patent number: 10544419
    Abstract: Aptamers having improved stability against nucleases that bind PDGF and aptamers that bind VEGF are provided. In addition, aptamer constructs comprising a PDGF aptamer and a VEGF aptamer are provided. Pharmaceutical compositions comprising the aptamers and aptamer constructs are provided, as well as methods of treating conditions using the aptamers and aptamer constructs.
    Type: Grant
    Filed: May 10, 2018
    Date of Patent: January 28, 2020
    Assignee: SomaLogic Inc.
    Inventors: Nebojsa Janjic, Daniel W. Drolet, Amy D. Gelinas, Chi Zhang, Michael Vrkljan
  • Patent number: 10526648
    Abstract: The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: January 7, 2020
    Assignee: Illumina Cambridge Limited
    Inventors: Elena Cressina, Antoine Francais, Xiaohai Liu
  • Patent number: 10526646
    Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
    Type: Grant
    Filed: December 19, 2016
    Date of Patent: January 7, 2020
    Assignee: Agilent Technologies, Inc.
    Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
  • Patent number: 10519184
    Abstract: The present invention relates to a 5-formylcytosine specific chemical labeling method and related applications in aspects such as sequencing, detection, imaging, and diagnosis. In the method, a condensation reaction occurs between an active methylene group in an active methylene compound containing a side-chain reactive group and an aldehyde group in 5-formylcytosine or a 1-substituted derivative of 5-formylcytosine, and at the same time an intramolecular reaction occurs between the side-chain reactive group of the active methylene compound and a 4-amino group of cytosine to implement ring closing.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: December 31, 2019
    Assignee: PEKING UNIVERSITY
    Inventors: Chengqi Yi, Bo Xia, Ankun Zhou
  • Patent number: 10519485
    Abstract: The present invention provides nucleic acid probes comprising two quenchers of excited state energy, and methods of their use.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: December 31, 2019
    Assignee: BIOSEARCH TECHNOLOGIES, INC.
    Inventors: Ronald M. Cook, Ben Ayer, Marc P. Beal
  • Patent number: 10519500
    Abstract: A method of preparing a crystalline copper-based coordination polymer comprises preparing a mixture of copper ions and a first quaternized carboxylate pyridyl ligand; adding a second polypyridyl ligand; and forming crystals of a copper-based coordination polymer therefrom. The crystalline copper-based coordination polymer is suitable for providing a sensing platform for detecting the presence of one or more target nucleic acid sequences such as viral RNA, in particular Dengue virus and/or Zika virus RNA with high selectivity and high specificity. A method of detecting at least a first target nucleic acid sequence, in particular from a viral RNA, in particular it is Flavivirus RNA, in a sample is also provided. Further provided is a kit including the crystalline copper-based coordination polymer and an oligonucleotide probe.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: December 31, 2019
    Assignees: MACAU UNIVERSITY OF SCIENCE AND TECHNOLOGY, SOUTHERN MEDICAL UNIVERSITY
    Inventors: Jin-Xiang Chen, Bao-Ping Xie, Gui-Hua Qiu, Pei-Pei Hu, Zhen Liang, Ye-Mei Liang, Bin Sun, Li-Ping Bai, Zhi-Hong Jiang
  • Patent number: 10519484
    Abstract: The present invention relates to the technical field of biology, and in particular relates to a lytic composition and an application thereof, a kit, a method for preparing nucleic acid by utilizing the lytic composition, and a nucleic acid analysis method. A lytic product obtained in the present invention can be directly used for the nucleic acid analysis method without purifying the nucleic acid. According to the lytic composition disclosed by the present invention, multiple biological tissues and cells are lysed so as to release the nucleic acid of the cells into a solution, the nucleic acid in a whole-cell lysing and mixing state directly serves as a template without using a conventional nucleic acid separation and purification process after termination of the reaction, and nucleic acid amplification analysis is guided. For example, nucleic acid amplification is performed by a real-time fluorescence qPCR, etc.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: December 31, 2019
    Assignees: HEALTH & HELP BIOSCIENCE CO., LTD
    Inventors: Xiaohui Wang, Dongjie Sun, Qingqing Zhuang, Lihan Jiang, La Sa
  • Patent number: 10519496
    Abstract: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: December 31, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: Shankar Balasubramanian, Colin Lloyd Barnes, Xiaohai Liu, Xiaolin Wu, John Milton
  • Patent number: 10513704
    Abstract: This invention provides for processes for binding to and/or chemically transforming a preselected target, where the process involves contacting said target to an oligonucleotide molecule that contains one or more “non-standard” nucleotides, which are nucleotide analogs that, when incorporated into oligonucleotides (DNA or RNA, collectively xNA), present to a pattern of hydrogen bonds that is different from the pattern presented by adenine, guanine, cytosine, and uracil. This disclosure provides an example where such an oligonucleotide molecule is built from both D- and L-mirror image carbohydrates in the backbone. It also provides a process for obtaining these binders and/or transformers by a laboratory in vitro selection process that exploits rolling circle amplification rather than the polymerase chain reaction.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: December 24, 2019
    Inventor: Steven A Benner
  • Patent number: 10513731
    Abstract: The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3?-OH blocking group covalently attached thereto, such that the 3? carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R?)2-O—R?, —C(R?)2-N(R?)2, —C(R?)2-N(H)R?, —C(R?)2-S—R? and —C(R?)2-F, wherein each R? is or is part of a removable protecting group; each R? is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R?)2 represents an alkylidene group of formula ?C(R??)2 wherein each R?? may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R? is exchanged for H or, where Z is —C(R?)2-F, the F is ex
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: December 24, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: John Milton, Xiaolin Wu, Mark Smith, Joseph Brennan, Colin Barnes, Xiaohai Liu, Silke Ruediger
  • Patent number: 10508129
    Abstract: Cyclic-GMP-AMP synthase (cGAS) and cyclic-GMP-AMP (cGAMP), including 2?3-cGAMP, 2?2-cGAMP, 3?2?-cGAMP and 3?3?-GAMP, are used in pharmaceutical formulations (including vaccine adjuvants), drug screens, therapies, and diagnostics.
    Type: Grant
    Filed: May 7, 2019
    Date of Patent: December 17, 2019
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Zhijian Chen, Lijun Sun, Jiaxi Wu, Heping Shi, Chuo Chen
  • Patent number: 10508116
    Abstract: Disclosed herein are Click Nucleic Acid Polymers (CNA-polymers) that comprise repeating dimer, trimer and tetramer units. The disclosed polymers can be used for antisense applications, for example, in treatment of “trinucleotide repeat disorders, i.e., Huntington's Disease and the like.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: December 17, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY
    Inventors: Christopher N. Bowman, Weixian Xi, Sankha Pattanayak
  • Patent number: 10494661
    Abstract: Disclosed are a stabilizer for storage of a biological sample, a composition for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions, a kit comprising the composition, a mixture comprising the composition and a biological sample, and a method for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions. The integrity and stability of the nucleic acid and/or polypeptide in a biological sample can be stored for a long time under non-freezing conditions by using the stabilizer according to the invention, and therefore the stabilizer has a good application prospect.
    Type: Grant
    Filed: January 27, 2015
    Date of Patent: December 3, 2019
    Assignee: BGI SHENZHEN
    Inventors: Qiang Feng, Mo Han, Liang Xiao, Jun Wang
  • Patent number: 10487102
    Abstract: The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterized in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: November 26, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, John Milton, Silke Ruediger