Patents Examined by Johnnie R. Brown
  • Patent number: 5449769
    Abstract: A composition suitable for sulfurizing an organophosphite. The reagent comprises a solution of sulfur and a tertiary amine, in a suitable solvent such as carbon disulfide. The tertiary amine is preferably an optionally substituted trialkyl amine, for example triethylamine or diisopropylamine. A method is also provided in which phosphites or phosphonous esters are sulfurized to their corresponding phosphorothioates or phosphonothioates, respectively, using compositions of the present invention. This method has particular application to produce internucleotide phosphorothioate or phosphonothioate bonds in a nucleotide multimer.
    Type: Grant
    Filed: March 6, 1989
    Date of Patent: September 12, 1995
    Assignee: Gen-Probe Incorporated
    Inventor: Ram S. Bhatt
  • Patent number: 5447848
    Abstract: Nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium are described along with methods utilizing such probes for the detection of Campylobacter in clinical, food and other samples.
    Type: Grant
    Filed: July 7, 1988
    Date of Patent: September 5, 1995
    Assignee: AMOCO Corporation
    Inventors: Susan M. Barns, Ray A. McMillian, David J. Lane, Mark L. Collins, James E. Awell, Ayoub Rashtchian
  • Patent number: 5420036
    Abstract: The present invention relates to Bacillus polymyxa Haitai 1 (KCCM-10001) capable of producing polysaccharides which are viscous and thermally stable, and have the capacities of water holding, film forming and emulsifying, and can reversibly form a gel when heated, and to polysaccharides having the above characteristics which are produced by utilizing the said microorganism.
    Type: Grant
    Filed: April 16, 1993
    Date of Patent: May 30, 1995
    Assignees: Haitai Confectionery Co., Ltd., Ju H. Yu
    Inventors: Ju H. Yu, Yong M. Shin, Kyu S. Rhee
  • Patent number: 5414078
    Abstract: A process for the preparation of 3'-substituted-2',3'-dideoxynucleosides and 2'-deoxynucleosides is provided that utilizes inexpensive, non-carbohydrate, acyclic, achiral starting materials and that proceeds with high enantiomeric and stereochemical control. The method can be used to prepare the pharmaceutically important compounds 3'-azido-3'-deoxythymidine, 3'-azido-2',3'-dideoxyuridine and 3'-fluoro-3'-deoxythymidine.
    Type: Grant
    Filed: April 5, 1991
    Date of Patent: May 9, 1995
    Assignee: Emory University
    Inventors: Dennis C. Liotta, Michael W. Hager
  • Patent number: 5405838
    Abstract: A topical powder composition containing a cyclic AMP derivative and a water-absorptive powdery material. The topical powder composition is useful for wet dermatoses. Release of drug can be controlled.
    Type: Grant
    Filed: September 27, 1991
    Date of Patent: April 11, 1995
    Assignee: Daiichi Pharmaceutical Co., Ltd.
    Inventors: Norio Takasugi, Eiichi Mafune, Toshiyuki Takayasu
  • Patent number: 5384396
    Abstract: An efficient process for the deoxygenation of 2'- and or 3'-hydroxyl groups of a nucleoside that includes reacting the hydroxyl group with 3-halopropionitrile or 2-nitroethylhalide and carbon disulfide in base to form a 2'- or 3'-(cyanoethylthio or nitroethylthio)thiocarbonyl, that is reductively eliminated and replaced with hydrogen. The deoxygenation process can be used in a wide variety of nucleoside syntheses that require the elimination of the 2'- or 3'-hydroxyl groups, including the preparation of 3'-substituted-2',3'-dideoxynucleosides such as 3'-azido-3'-deoxythymidine and 3'-azido-2',3'-dideoxyuridine.
    Type: Grant
    Filed: March 7, 1991
    Date of Patent: January 24, 1995
    Assignee: The University of Georgia Research Foundation, Inc.
    Inventors: Chung K. Chu, Yaoquan Chen
  • Patent number: 5366964
    Abstract: A viscoelastic solution including a buffered solution, 0.01-10% chondroitin sulfate, 0.01-10% hydroxypropyl methylcellulose, and 0.01-10% sodium hyaluronate, pH adjusted to 6.0-8.0 at a osmolarity between 200-400 mOsmol/L. The buffered solution can be HEPES buffered minimal essential medium (MEM), phosphate buffered saline (PBS), or buffered balanced salt solution. A cell growth factor or cell growth supplement can also be included in the solution.
    Type: Grant
    Filed: November 13, 1989
    Date of Patent: November 22, 1994
    Inventors: Richard L. Lindstrom, Debra Skelnik
  • Patent number: 5367066
    Abstract: A modified polynucleotide containing at least one cleavable or abasic site as shown below. ##STR1## DNA.sub.1 is a first segment of DNA; DNA.sub.2 is a second segment of DNA; and R.sub.m is C.sub.1 to C.sub.16 alkylene or an oxytheylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an interger in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR2## Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.
    Type: Grant
    Filed: July 24, 1991
    Date of Patent: November 22, 1994
    Assignee: Chiron Corporation
    Inventors: Michael S. Urdea, Thomas Horn
  • Patent number: 5364466
    Abstract: A polymer scale preventive agent for use in polymerization of a monomer having an ethylenically unsaturated double bond, comprising (A) a nitrogen-containing organic compound having at least five continuous conjugated .pi. bonds, (B) an anionic organic compound having at least one group selected from the group consisting of the sulfonic acid group and the carboxyl group and having at least five continuous conjugated .pi. bonds, and (C) a water-soluble basic polysaccharide. The agent is used for forming a coating on the inner wall, etc. of a polymerization vessel. Deposition of polymer scale can be effectively prevented not only in the liquid phase area but also in the vicinity of the interface between the gas and liquid phases. Products formed from polymer obtained have very few fish eyes.
    Type: Grant
    Filed: April 8, 1992
    Date of Patent: November 15, 1994
    Assignee: Shin-Etsu Chemical Co., Ltd.
    Inventors: Toshihide Shimizu, Minoru Shigemitsu
  • Patent number: 5362863
    Abstract: A 4"-oxoavermectin intermediate is reductively aminated using hexa- or heptamethyldisilazane and sodium borohydride to produce a 4"-amino (or methylamino)avermectin. The 4"- aminoavermectin is a useful intermediate in the preparation of N-acyl derivatives. The compounds are useful as agricultural and animal health insecticides and parasiticides.
    Type: Grant
    Filed: September 29, 1993
    Date of Patent: November 8, 1994
    Assignee: Merck & Co., Inc.
    Inventor: Raymond Cvetovich
  • Patent number: 5362861
    Abstract: A continuous method of bleaching an alkylpolyglycoside, substantially free of alcohol, with peroxy compounds, preferably hydrogen peroxide, which is highly efficient to provide an unexpected high degree of color reduction from a dark brown to a light, white product, from an extinction coefficient color respectively of about 10 to about 15 to about 0.025 to about 0.15. The bleaching is carried out at controlled pH and temperature, under pressure preferably in the presence of Mg or MgO.
    Type: Grant
    Filed: July 15, 1992
    Date of Patent: November 8, 1994
    Assignee: Henkel Corporation
    Inventors: Patrick M. McCurry, Jr., Robert L. Klein, Jr., Michael W. Gibson, James D. Beaulieu, Janet R. Varvil
  • Patent number: 5362866
    Abstract: A versatile polymeric support system for the synthesis of oligonucleotides is provided featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolating and detecting complementary polynucleic acids.
    Type: Grant
    Filed: March 17, 1992
    Date of Patent: November 8, 1994
    Assignee: Molecular Biosystems, Inc.
    Inventor: Lyle J. Arnold, Jr.
  • Patent number: 5362862
    Abstract: An improved process for formation of a 5-oxime on avermectin derivatives comprises the treatment of the oxo compound with O-(trimethylsilyl)hydroxylamine in the presence of a Lewis acid.
    Type: Grant
    Filed: September 29, 1993
    Date of Patent: November 8, 1994
    Assignee: Merck & Co., Inc.
    Inventors: Joseph S. Amato, Raymond Cvetovich
  • Patent number: 5358936
    Abstract: A glucofuranose derivative substituted at the 3,5,6- or 3,6-positions with a radical that provides an anionic charge at physiological pH values is disclosed, as are pharmaceutical compositions, and methods of making and using the same. The compounds are useful in treating inflammation, and particularly conditions that involve neutrophil influx; they are also useful in inhibiting gastric ulcer formation.
    Type: Grant
    Filed: July 31, 1991
    Date of Patent: October 25, 1994
    Assignee: Paul Gordon
    Inventors: Paul Gordon, Edward P. Gamson
  • Patent number: 5359051
    Abstract: Compositions and methods for modulating the activity of RNA are disclosed. In accordance with preferred embodiments, antisense compositions are prepared comprising targeting and reactive portions. In preferred embodiments, the reactive portions comprise one or two imidazole functionalities conjugated to the targeting oligonucleotide via linkers with and without intervening intercalating moieties and act through phosphorodiester hydrolytic bond cleavage. Therapeutics, diagnostics and research methods are also disclosed. Synthetic nucleosides and nucleoside fragments are also provided which are useful for elaboration of oligonucleotides for such purposes.
    Type: Grant
    Filed: March 5, 1992
    Date of Patent: October 25, 1994
    Assignee: ISIS Pharmaceuticals
    Inventors: Phillip D. Cook, Charles J. Guinosso, Thomas Bruice
  • Patent number: 5356880
    Abstract: The invention provides a novel glycoside which contains as the aglycon a 4-(substituted phenyl)piperazine-1-yl derivative of glycyrrhetinic acid and 11-deoxo-glycyrrhetinic acid or their derivative as well as a composition for the treatment of virus infection, which contains these compounds as a main active component.
    Type: Grant
    Filed: May 28, 1992
    Date of Patent: October 18, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Yoshiro Ishiwata, Syoji Yokochi, Kyoichi Asano, Takahiko Mitani, Takuji Kakigami, Noriyuki Iwata, Kougaku Isogawa, Yutaka Baba, Hiroyuki Ohwaki, Kiichi Sawai, Hiromoto Kimura, Masato Fukushima, Ryoichi Unno, Tamaki Ohtuka
  • Patent number: 5354852
    Abstract: A particulate aromatic or araliphatic carbamate of a polysaccharide having a mean particle diameter of 1 to 200 .mu.m and a specific surface area of 0.5 to 300 m.sub.2 /g is prepared by first dissolving an aromatic or araliphatic carbamate of a polysaccharlde in an organic solvent, adding a hydrocarbon having 4 to 22 carbon atoms in an amount of 0 to 0.5 time by volume of that of the organic solvent, gradually adding the resultant solution to a sufficiently agitating aqueous surfactant solution, removing the organic solvent while continuing the agitation, isolating solid particles, and washing and drying the solid particles. The particulate aromatic or araliphatic carbamate of a polysaccharide has an excellent capability of resolution and, as such, can be used as a stationary phase of preparative liquid chromatography without being supported on a support.
    Type: Grant
    Filed: October 27, 1992
    Date of Patent: October 11, 1994
    Assignee: Daicel Chemical Industries, Ltd.
    Inventor: Hirokazu Ikeda
  • Patent number: 5350839
    Abstract: Erythromycins showing an extremely relieved bitterness at administration and an improved absorbability in vivo when orally administered are provided.A compound having a group represented by the following formula:--O--CO--O--[(CH.sub.2).sub.m --O].sub.n --R(wherein R represents an alkyl group having from 1 to 12 carbon atoms, m represents an integer of from 2 to 4, and n represents an integer of from 1 to 7) at the 2'-position of erythromycins or a salt thereof.
    Type: Grant
    Filed: April 15, 1993
    Date of Patent: September 27, 1994
    Assignee: Taisho Pharmaceutical Co., Ltd.
    Inventors: Toshifumi Asaka, Yoko Misawa, Masato Kashimura, Shigeo Morimoto, Yoshiaki Watanabe, Katsuo Hatayama
  • Patent number: 5350524
    Abstract: Mixed polysaccharide compositions, well adopted for flocculating/precipitating solid particulates from liquid dispersions thereof and for the shaping of insulating articles therefrom, comprise (i) at least one succinoglycan polysaccharide, the basic recurring structural unit of which comprising glucose, galactose and succinyl moieties, and (ii) at least one natural polysaccharide or derivative thereof.
    Type: Grant
    Filed: July 30, 1991
    Date of Patent: September 27, 1994
    Assignee: Rhone-Poulenc Chimie
    Inventors: Marie-Madeleine Besnard, Claire David, Magali Knipper
  • Patent number: 5348946
    Abstract: Heterocyclic antitumor compounds are described, which are useful in the treatment of cancer and tumors in vitro, such as breast cancer, leukemia, lung cancer, colon cancer, ovarian cancer, renal cancer, CNS cancer and melanoma. Pharmaceutical compositions and method of preparing the compounds are also described.
    Type: Grant
    Filed: August 28, 1991
    Date of Patent: September 20, 1994
    Assignee: Biochem Immunosystems, Inc.
    Inventors: Giorgio G. Attardo, Bernard Belleau, deceased