Abstract: An incubator includes: an insulated box including an incubation space surrounded by multiple inner faces; a humidifying unit that humidifies an incubation space; a first heating unit that heats a first inner face among the multiple inner faces, with electric power supplied; a second heating unit that heats a second inner face, different from the first inner face, with electric power supplied; and a control unit that controls the magnitude of electric power supplied to each of the first heating unit and the second heating unit. The control unit repeatedly changes the magnitude of electric power supplied to the first heating unit at first timing, and also repeatedly changes the magnitude of electric power supplied to the second heating unit at second timing different from the first timing.

Abstract: Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.

Abstract: A novel vial for holding a segment of epithelial tissue is provided. The vial is easy to assemble and allows horizontal alignment of the tissue sample. A device comprising the vial, to methods for generating the device, and to a multitude of said devices which allow medium throughput measurements of absorption, transport and/or secretion across an epithelial tissue are also provided.

Abstract: The present application provides a reactor with an inclined bottom gas-inlet for aerobic fermentation. A fermenter is provided with a circular inner tank, end covers and a jacket. An airtight fermentation space is formed in the fermenter by the inner tank, an upper end cover and a lower end cover. A feed opening and an exhaust outlet are arranged at an upper part of the fermenter, and a discharge opening is arranged at a lower part of the lower end cover of the fermenter. An energy-saving stirrer is mounted in the fermenter. A plurality of air chambers are arranged at an external wall at the bottom of the inner tank of the fermenter and are within the jacket. A plurality of aeration nozzles are defined on an inner side of each air chamber, and the aeration nozzles are disposed next to the inner tank.

Abstract: An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.

Abstract: Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment.

Abstract: An organ-chimerization maintenance device. The organ-chimerization device includes at least one large cannula connector that connects to at least one of an organ's vessels. The large cannula is configured to supply a chimerization solution to the organ. The organ-chimerization device also includes a an oxygenation cannula connector that facilitates oxygenation of the organ during chimerization.

Abstract: A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Abstract: A disposable polymer container for manipulation of small specimens adapted to optimize heat transfer between an external heating element and specimens herein contained. In particular, this invention relates to the field of containers for Assisted Reproductive Technology hereunder In-vitro fertilization (IVF).

Abstract: A process to treat a gas comprising hydrogen sulphide and mercaptans is described. The following steps are part of this process: (a) contacting the hydrogen sulphide and mercaptans comprising gas (1) with an aqueous solution (3) comprising sulphide-oxidising bacteria thereby obtaining a loaded aqueous solution (5) and a gas (4) having a lower content of hydrogen sulphide and mercaptans, (b) contacting the loaded aqueous solution with mercaptan reducing microorganisms immobilized on a carrier under anaerobic conditions, (c) separating the aqueous solution obtained in step (b) from the mercaptan reducing microorganisms to obtain a first liquid effluent (7), and (d) contacting the first liquid effluent (7) with an oxidant (9) to regenerate the sulphide-oxidising bacteria to obtain a second liquid effluent (11) comprising regenerated sulphide-oxidising bacteria. The sulphide-oxidising bacteria as present in step (a) are comprised of regenerated sulphide-oxidising bacteria obtained in step (d).

Abstract: Provided is a bubble-jetting member, the tip section of which is not damaged even when high voltage is applied thereto. There can be provided a bubble jetting member comprising: a core that is formed of a conductive material; a shell part that is formed of an insulating material, includes an extended section that extends beyond the tip of the core, and is closely adhered at least partially to the core and covers the core; and a space that is formed between the extended section and the tip of the core and has a bubble-jetting port, wherein there is formed on the tip of the extended section a thick portion that is thicker than the rest of the extended section whereby the tip section is not damaged.

Abstract: A method of collecting target cells including providing a cell collection device comprising a conduit defining a fluid channel, a first screen and a second screen, the first and second screens are movable relative to each other along the flow direction between a first configuration and a second configuration, flowing a fluid composition comprising the target cells in the flow direction while the first and second screens are in a first configuration to screen at least part of the target cells, moving the first screen relative to the second screen along the flow direction from the first configuration to form the second configuration, to permit at least part of the target cells screened in the first configuration to pass the first screen first and then the second screen, and collecting at least part of the target cells at the second end of the fluid channel.

Abstract: A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.

Abstract: An electroporation apparatus and its novel chamber with inlet ports for mixing cells and exogenous material. The inlet ports are oriented in nonparallel to each other immediately adjacent at the same top corner of the first wall of the chamber. The mixing chamber comprises successive wall sections, two curved walls at its bottom; the first curved corner is on the same side of the chamber where the liquids enter the chamber, and directs the liquids to the second curved corner at the opposing side of the chamber which in turn further redirects the mixing to the first curved corner. The direction of the liquid flow mixture change direction at least twice into the mixing chamber.

Abstract: A luminometer apparatus includes, inside a light-shielded room, a container rack loaded with holding containers, a dispensation mechanism that dispenses a liquid, a rotary plate that turns, while holding a plurality of reaction containers which accommodate a mixture liquid of a sample and a luminescent reagent on a concentric annular plate, and is provided with a gap allowing the container rack to pass therethrough between at least a pair of adjacent ones of the reaction containers, and a photodetection unit that performs luminescence measurements. The light-shielded room has an insertion opening having a width allowing for insertion of the container rack and provided to be openable and closable. The rotary plate is provided with a region in which the container rack can be installed inside a region of passage of the reaction containers, when turning, and is provided to be turnable where the light-shielded room is in a closed state.

Abstract: A cell aggregate culture vessel (1) is a vessel for culturing a cell aggregate. The cell aggregate culture vessel (1) includes a well (21) having a culture space capable of storing the cell aggregate and culture fluid and a tubular body (3) which is disposed on the well on a plane at which the well has an opening and have an inner cavity communicating with the culture space, and one or more communication portion (3a) capable of discharging the culture fluid without allowing passage of the cell aggregate to the outside of the tubular body (3) is formed in a tubular wall of the tubular body (3). The communication portion (3a) may be, for example, a slit having a width of 0.1 mm or larger and 0.5 mm or smaller.

Abstract: Devices and methods cell culture are disclosed herein. In particular embodiments, the cell culture devices include multi-channel devices with a non-linear flow path that recapitulates the three-dimensional microarchitecture and physiological organ-level functions of human organs with cellular and molecular resolution.

Abstract: An apparatus to prevent bio-filming during the delivery of photonic energy to a culture or set of organisms in a fluid culture medium, the device including a culture medium, a container configured to house the culture medium, and an assembly to generate and deliver a liquid energy transfer medium between the culture medium and the photonic device, the assembly including one or more apertures sized and shaped to form one or more liquid replenishing lenses and a fluid-light unit in the container to house the photonic device and the liquid energy transfer medium, the photonic device is at least one from among a photoreceptor, a photoemitter, or a combination of the photoreceptor and the photoemitter, and further including a pump coupled to the fluid-light unit, the assembly is configured to convey the photonic energy between the photonic device and the culture medium through the liquid energy transfer medium.

Abstract: To provide a thermal cycling system allowing an efficient thermal cycling and an optical detection during the diagnostic process a thermal cycling system is proposed, comprising: at least one heating device (10a, 10b) having a transparent substrate (11a, 11b) and a heating element (12a, 12b), and a chamber (30) adapted to receive a sample, the chamber (30) is placed adjacent to at least one heating device (10a, 10b), wherein at least a part of the chamber (30) comprises a transparent area (31) aligned with the transparent substrate (11a, 11b) of the at least one heating device (10a, 10b). Thereby, the speed and efficiency of the thermal system is increased. Moreover, an optical detection of the sample is possible.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.