Patents Examined by Lionel M. Shapiro
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Patent number: 4820640Abstract: A method of culturing Lipomyces, such as Lipomyces starkeyi, at pH of 2.5-4.5 and producing extracellular dextranase with co-production of alpha-glucosidase so that the dextranase containing product is essentially free from contamination.Type: GrantFiled: March 10, 1986Date of Patent: April 11, 1989Assignee: Louisiana State University and Agriculture and Mechanical CollegeInventors: Donal F. Day, David W. Koenig
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Patent number: 4777134Abstract: An inositoltriphosphate (IP.sub.3) selected from the group consisting of D-myo-inositol-1.2.6-triphosphate, D-myo-inositol-1.2.5-triphosphate, L-myo-inositol-1.3.4-triphosphate and myo-inositol-1.2.3-triphosphate, a method for producing the same and a composition comprised of the same.Type: GrantFiled: October 18, 1985Date of Patent: October 11, 1988Inventor: Matti Siren
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Patent number: 4770997Abstract: Thermostable bilirubin oxidase having substrate specificity to at least bilirubin and capable of catalyzing a reaction in which biliverdin and water are formed from bilirubin and oxygen. It can be produced by culturing a bilirubin oxidase producing micro-organism belonging to the genus Bacillus, for example, Bacillus licheniformis and then preparing the resultant bilirubin oxidase from the cultured broth.Type: GrantFiled: January 21, 1986Date of Patent: September 13, 1988Assignee: Toyo Jozo Co., Ltd.Inventors: Eiichi Yoshino, Shigeyuki Imamura, Kazuo Matsuura, Hideo Misaki
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Patent number: 4771003Abstract: Novel enzymes exhibiting proteolytic activity in alkaline media and stability at high temperatures and under alkaline conditions are produced by a novel Bacillus strain designated GX6638 or its mutants or variants. These enzymes are especially well-suited for inclusion in washing compositions. A culture of GX6638 has been deposited with the American Type Culture Collection, Rockville, Md. as ATCC No. 53278.Type: GrantFiled: October 22, 1985Date of Patent: September 13, 1988Assignee: Genex CorporationInventors: Edmund J. Stellwag, Donald R. Durham, Wayne E. Swann, Carol A. Nolf, David B. Stewart
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Patent number: 4766071Abstract: The invention relates to a process for the enzymatic preparation or regeneration of coenzymes selected from the group consisting of ATP, acetyl-CoA, acetyl-phosphate, NAD, NADP, NADH, and NADPH from their known oxidized and reduced forms, in which alkanals, such as acetaldehyde, are used as an oxidizing agent or alkanals such as acetaldehyde and alcohols, such as ethanol, are used as a reducing agent and a cell lysate of Clostridium kluyveri is used as a biocatalyst.Type: GrantFiled: February 14, 1986Date of Patent: August 23, 1988Assignee: Henkel Kommanditgesellshaft auf AktienInventors: Helmut Simon, Alexander Deffner
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Patent number: 4766074Abstract: Disclosed is a method for increasing the milk clotting ability and reducing the thermal stability of microbial rennet from Rhizomucor pusillus. The method involves treating the rennet with a methionine oxidizing composition and the anhydride of a dicarboxylic organic acid.Type: GrantFiled: October 13, 1987Date of Patent: August 23, 1988Assignee: Miles Inc.Inventors: Herbert J. Havera, John D. Humphreys
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Patent number: 4764470Abstract: A novel enzyme exhibiting proteolytic activity in alkaline media and stability under alkaline conditions is produced by a novel Bacillus strain designated GX6644 or by its mutants or variants. The enzyme is well-suited for inclusion in washing compositions. A culture of GX6644 has been deposited with the American Type Culture Collection, Rockville, Maryland as ATCC No. 53441.Type: GrantFiled: February 5, 1986Date of Patent: August 16, 1988Assignee: Genex CorporationInventors: Donald R. Durham, Edmund J. Stellwag, Clyde G. McNamee
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Patent number: 4764468Abstract: The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, there is added, as activity stabilizer, phenol which optionally contains one or more substituents selected from lower alkyl radicals and chlorine and bromine atoms, in an amount of from 0.0005 to 2% by weight, referred to the solution.Type: GrantFiled: March 4, 1986Date of Patent: August 16, 1988Assignee: Boehringer Mannheim GmbHInventors: Rainer Wehner, Johann Mattersberger, Dagmar Klenner, Gerlinde Deutsch-Weyer
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Patent number: 4762789Abstract: The present invention is a composition of matter composed essentially of a purified homogeneous thiol proteinase secreted by Entamoeba histolytica trophozoites. In purified homogeneous form the neutral thiol proteinase has a subunit molecular weight of about 56,000.+-.4,000, a neutral pH optimum, and an isoelectric point of about 6. The purified neutral thiol proteinase of the present invention can be obtained by using anion exchange and chromatofocusing Fast Protein Liquid Chromatography (FPLC) to purify the major neutral proteinase from secretions of axenically cultured Entamoeba histolytica trophozoites.The homogeneous neutral thiol proteinase of the present invention is immunogenic. It is useful as a serodiagnostic reagent for identifying the presence of antibodies in the sera of individuals exposed to Entamoeba histolytica trophozoites. The purified homogeneous enzyme can be used in ELISA assays or in other standard immunoassays.Type: GrantFiled: January 16, 1986Date of Patent: August 9, 1988Assignee: The University of CaliforniaInventors: William E. Keene, James H. McKerrow
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Patent number: 4761374Abstract: A thermally stable tryptophanase having (1) an optimum temperature for activity at a pH of 8.0 of about 70.degree. C., and (2) such thermal stability that it is not thermally deactivated when maintained at temperatures up to about 65.degree. C. and a pH of 8.0 for 40 minutes. The thermally stable tryptophanase can be produced by cultivating in a tryptophan-containing culture medium a thermally stable tryptophanase-producing bacterium which does not grow alone in said medium but grows there in the presence of Bacillus sp. strain S, and obtaining the resulting thermally stable tryptophanase from the culture broth. The thermally stable tryptophanase-producing microorganism for use in the above process is a novel organism.Type: GrantFiled: June 6, 1986Date of Patent: August 2, 1988Assignee: Teruhiko BeppuInventors: Teruhiko Beppu, Seibun Suzuki
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Patent number: 4758518Abstract: An enzyme composition comprising a D-2-haloalkanoic acid halidohydrolase, bacteria containing said enzyme, a process for the preparation of said enzyme as a cell-free composition and a process in which the enzyme is used to increase the concentration of the L-enantiomer in a mixture of the D- and L-enantiomers of a 2-haloalkanoic acid. Preferably the 2-haloalkanoic acid is 2-bromo- or 2-chloro-propionic acid and the process for increasing the concentration is carried out under anaerobic conditions.Type: GrantFiled: October 21, 1985Date of Patent: July 19, 1988Assignee: Imperial Chemical Industries PLCInventor: Stephen C. Taylor
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Patent number: 4758515Abstract: Process for the preparation of a low-glucose digestion product from inulin-containing parts of plants, the parts of plants being comminuted, and the inulin being degraded to fructose or fructose oligomers. The process comprises a suspension of the comminuted parts of plants in an aqueous medium, or a pressed juice or extract obtained therefrom, being heated, without removal of the inulin, at temperatures up to 100.degree. C., until the enzymes intrinsic to the plant have substantially been inactivated, whereupon, after cooling, inulinase is added to the suspension or the pressed juice or the extract.Type: GrantFiled: March 7, 1986Date of Patent: July 19, 1988Assignee: Gunter BarwaldInventors: Gunter Barwald, Erhard F. Flother
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Patent number: 4758517Abstract: A process is provided for increasing the yield of active cells of Treponema hyodysenteriae by growing such cells in a nutrient medium containing a cholesterol-rich bovine fraction. Killed cells grown in this manner can be used to produce a bacterin which is effective against swine dysentery.Type: GrantFiled: October 28, 1986Date of Patent: July 19, 1988Assignee: Mobay CorporationInventor: Richard E. Parizek
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Patent number: 4757015Abstract: Phenylalanine ammonia-lyase (PAL) producing Rhodotorula graminis, high PAL specific activity, strain GX 6000 its progeny, and PAL producing mutants thereof can be utilized for phenylalanine production from cinnamate. In this strain, PAL can be induced synergistically by phenylalanine and leucine or isoleucine.Type: GrantFiled: August 11, 1986Date of Patent: July 12, 1988Assignee: Genex CorporationInventors: Steve A. Orndorff, Don R. Durham
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Patent number: 4757016Abstract: The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, when the peroxidase is used as a conjugate with an immunologically effective substance, there is added, as activity stabilizer, aminopyrine in an amount of from 0.0005 to 2% by weight, referred to the solution.Type: GrantFiled: March 4, 1986Date of Patent: July 12, 1988Assignee: Boehringer Mannheim GmbHInventors: Dagmar Klenner, Gerd Kleinhammer, Rolf Deeg
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Patent number: 4753881Abstract: Deacetoxycephalosporin C synthetase is provided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chromatography, all carried out in the presence of glycerol, a C.sub.1 -C.sub.3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithiothreitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.Type: GrantFiled: September 10, 1986Date of Patent: June 28, 1988Assignee: Eli Lilly and CompanyInventors: Wu-Kuang Yeh, Joe E. Dotzlaf
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Patent number: 4753882Abstract: Different from conventional urease products, the urease of the present invention has excellent stability, and has a smaller Km value. The urease of this invention is produced microbiologically by a thermophilic microorganism belonging to the genus of Bacillus and especially named as Bacillus sp. TB-90 which is a novel species distinguishable from any of the microorganisms belonging to the genus of Bacillus.Type: GrantFiled: July 28, 1986Date of Patent: June 28, 1988Assignee: Sapporo Breweries LimitedInventors: Masachika Takashio, Yasuo Yoneda, Yutaka Mitani, Takahide Chikano, Minoru Kamimura
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Patent number: 4752579Abstract: Corn kernel hulls are found to be an essentially lignin-free material which is readily hydrolyzed to a mixture principally of three monosaccharides: D-glucose, D-xylose, and L-arabinose. Several discrete processing methods employing a combination of acid and enzymatic hydrolysis afford product streams rich either in glucose or in a mixture of the two pentoses, D-xylose and L-arabinose.Type: GrantFiled: October 21, 1985Date of Patent: June 21, 1988Assignee: UOP Inc.Inventors: Blaise J. Arena, Paul Allenza
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Patent number: 4752578Abstract: A method for the production of collagenase inducing factor is disclosed which comprises growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture medium at 35.degree.-38.degree. C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.Type: GrantFiled: April 11, 1986Date of Patent: June 21, 1988Assignee: Monsanto CompanyInventors: William M. Moore, Curtis A. Spilburg
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Patent number: 4749651Abstract: In an enzymatic saccharification process, a sugar solution of constant sugar content is obtained by adding to the starch-containing raw materials, prior to the enzymatic saccharification step, part of the sugar solution obtained after the saccharification step and being separated from solid material and having been brought to a higher concentration, so that a higher sugar content, in particular within the range from 15 to 22% by weight and preferably approximately 20% by weight, can constantly be maintained, independent of the concentration and quality of the raw materials.Type: GrantFiled: January 12, 1984Date of Patent: June 7, 1988Assignee: Voest-Alpine AktiengesellschaftInventors: Vilim Cvitas, Karl Faltejsek, Reinhart Hanke, Bertalan Treso