Patents Examined by Maryam Monshipouri
  • Patent number: 12018308
    Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: April 7, 2023
    Date of Patent: June 25, 2024
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
  • Patent number: 12012519
    Abstract: Liquid-liquid phase separation (LLPS) driven protein-based underwater adhesive coatings are made from a dimeric protein comprising a marine adhesive protein (MAP) domain and a liquid-liquid phase separation-mediating low complexity (LC) domain.
    Type: Grant
    Filed: February 8, 2021
    Date of Patent: June 18, 2024
    Assignee: ShanghaiTech University
    Inventors: Chao Zhong, Mengkui Cui
  • Patent number: 11987831
    Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.
    Type: Grant
    Filed: April 13, 2021
    Date of Patent: May 21, 2024
    Assignee: NOVOZYMES A/S
    Inventors: Randall Deinhammer, Joyce Craig, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong, Zhengfang Kang
  • Patent number: 11987832
    Abstract: Provided herein are compositions, methods, systems associated with propagation and fermentation, and co-products of biochemical production processes, for example, a DCO co-product resulting from converting oil containing grains into bio chemicals via fermentation in the presence of endogenous esterase. The DCO resulting from the processes exhibits lower metal ion content relative to a DCO obtained in the absence of endogenous fermentation with an esterase such as a lipase. The DCO is useful as a feedstock for the production of renewable diesel.
    Type: Grant
    Filed: August 5, 2021
    Date of Patent: May 21, 2024
    Assignee: POET Research, Inc.
    Inventors: Andrew J. Manning, Alex T. McCurdy, Steven T. Bly
  • Patent number: 11981946
    Abstract: Recombinant microorganisms configured for enhanced production of compounds such as 2-pyrone-4,6-dicarboxylic acid (PDC) and methods of using the recombinant microorganisms for the production of these compounds. The recombinant microorganisms include one or more modifications that reduce 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) cis-trans isomerase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) methyl esterase activity, and/or vanillate/3-O-methylgallate O-demethylase activity. The recombinant microorganisms can be used to generate PDC from media comprising plant-derived phenolics, such as syringyl phenolics, guaiacyl phenolics, and p-hydroxyphenyl phenolics. The plant-derived phenolics can be derived from pretreated lignin, including depolymerized lignin or other chemically altered lignin.
    Type: Grant
    Filed: May 6, 2021
    Date of Patent: May 14, 2024
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Daniel R. Noguera, Timothy James Donohue, Jose Perez, Wayne S. Kontur, German Eduardo Umana Chapeton
  • Patent number: 11981935
    Abstract: In various aspects and embodiments, the invention provides a nucleic acid molecule comprising a nucleotide sequence encoding a 7?-hydroxysteroid dehydrogenase (7?-HSDH) mutant that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid, wherein the mutant has, compared to the wildtype 7?-HSDH of SEQ ID NO:2, a decreased substrate inhibition and/or an altered cofactor usage, and the mutant has, in comparison with the wildtype 7?-HSDH of SEQ ID NO:2, 1 to 15 amino acid additions, substitutions, deletions and/or inversions in the sequence motif VMVGRRE corresponding to positions 36 to 42 of SEQ ID NO:2.
    Type: Grant
    Filed: January 15, 2021
    Date of Patent: May 14, 2024
    Assignee: Pharmazell GmbH
    Inventors: Dirk Weuster-Botz, Michael Braun, Arno Aigner, Boqiao Sun, Christina Kantzow, Sven Bresch, Daniel Bakonyi, Werner Hummel
  • Patent number: 11976316
    Abstract: Phenotyping of cells involves loading a biological sample into a microfluidic device (1, 100) comprising cell compartments (20, 120) to capture biological material, including target cells, in the sample in the cell compartments (20, 120). A subset (20A) of the cell compartments (20, 120) is identified as comprising target cells exhibiting target phenotype characteristic(s) as determined based on monitoring biological material in the cell compartments (20, 120) prior to addition of a test agent. The biological material is exposed to a test agent and a phenotypic response of the target cells to the test agent is determined based on monitoring target cells in the identified subset (20A) of the cell compartments (20, 120). The phenotyping of the target cells is thereby not overshadowed by the response of other cells and non-cell material present in the biological sample.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: May 7, 2024
    Inventors: Johan Elf, Michael Read, Özden Baltekin, Martin Lovmar, Petter Hammar, Elias Amselem, Mikael Olsson, Ove Öhman
  • Patent number: 11976309
    Abstract: In some aspects, the present invention provides methods and compositions for modifying target sites within nucleic acid molecules. In some embodiments, the methods comprise using adenosine deaminases that act on RNA (ADARs), and variants thereof, to modify target sites within DNA-RNA hybrid molecules. In other aspects, ADAR2 variant polypeptides as well as fusion proteins comprising an ADAR catalytic domain and a hybrid nucleic acid binding domain are provided, as are methods for use thereof. Methods for preventing and treating genetic disorders are also provided herein.
    Type: Grant
    Filed: June 13, 2022
    Date of Patent: May 7, 2024
    Assignee: The Regents of the University of California
    Inventors: Yuxuan Zheng, Claire Lorenzo, Peter Beal, Andrew Fisher, Leanna Monteleone
  • Patent number: 11965184
    Abstract: Engineered Cas9 systems are disclosed herein.
    Type: Grant
    Filed: February 10, 2021
    Date of Patent: April 23, 2024
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Fuqiang Chen
  • Patent number: 11959073
    Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
    Type: Grant
    Filed: April 29, 2021
    Date of Patent: April 16, 2024
    Inventors: Andrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
  • Patent number: 11959112
    Abstract: Cotton-containing textiles, such as “trash” feedstock in terms of end-of-life-cotton textiles, may be used to produce sugar without the same kinds of harsh pretreatments used for other biomasses, such as corn, grass sources, or wood. Disclosed is a process for production of sugar from a cotton-containing textile waste fabric comprising optionally mechanically pretreating the cotton-containing textile, pretreating the cotton-containing textile with an acid pretreatment to form a slurry, cooling the slurry, adding at least one base to the slurry, adding at least one additional acid to the slurry to form a buffer in situ, adding a hydrolysis enzyme, and optionally filtering the slurry.
    Type: Grant
    Filed: May 20, 2022
    Date of Patent: April 16, 2024
    Inventors: Matthew J. Farrell, Sha Fu, Mary A. Ankeny
  • Patent number: 11952398
    Abstract: A chromatographic method for collecting blood coagulation factor VII (Factor VII) and/or activated blood coagulation factor VII (activated Factor VII) from plasma-derived fractions with high yield is provided. In accordance with the method of the present invention, Factor VII and/or activated Factor VII of interest can be collected with a recovery rate of as high as 90% or more by letting Factor VII and/or activated Factor VII which fails to be adsorbed to a first anion exchange resin and remains in a non-adsorption fraction be adsorbed in a second anion exchange resin.
    Type: Grant
    Filed: June 9, 2021
    Date of Patent: April 9, 2024
    Assignee: KM Biologics Co., Ltd.
    Inventors: Kentaro Ebisu, Takashi Kai, Nozomi Matsuo
  • Patent number: 11946197
    Abstract: The present disclosure relates to a method for modifying polyester by a swelling agent combined with cutinase, belonging to the technical field of textile processing. According to the method, a phenol solution or an o-vanillin solution is used as the swelling agent to perform swelling treatment on the polyester, and combined with a Humicola insolens cutinase solution to perform hydrophilic modification on the polyester, which not only significantly improves the release amount of hydrolysates, but also reduces the fabric mass loss compared with the traditional chemical modification method.
    Type: Grant
    Filed: July 27, 2023
    Date of Patent: April 2, 2024
    Inventors: Jiajia Fu, Jundan Feng, Artur Cavaco-paulo, Rong Li, Yuzheng Lu, Xinming Zheng, Ruijuan Han, Chaoran Meng
  • Patent number: 11946080
    Abstract: Disclosed herein is one or more subtilisin variants, nucleic acids encoding same, and compositions and methods related to the production and uses thereof, including one or more subtilisin variants that has improved stability and/or soil removal compared to one or more reference subtilisin.
    Type: Grant
    Filed: June 18, 2017
    Date of Patent: April 2, 2024
    Assignee: DANISCO US INC.
    Inventors: Richard R. Bott, David A. Estell, Frits Goedegebuur, Harm Mulder, Sina Pricelius
  • Patent number: 11939613
    Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.
    Type: Grant
    Filed: September 14, 2021
    Date of Patent: March 26, 2024
    Assignee: BIOMEDICAN, INC.
    Inventors: Maxim Mikheev, Difeng Gao
  • Patent number: 11939616
    Abstract: The present disclosure provides, inter alia, compositions and methods for enforcing a pattern of cell surface fucosylated lactosaminyl glycans on a human cell. In certain embodiments, the compositions and/or methods utilize one or more members of the ?(1,3)-fucosyltransferase family. In certain embodiments, a process for custom-modifying a fucosylated lactosaminyl glycan on a human cell is disclosed.
    Type: Grant
    Filed: September 22, 2020
    Date of Patent: March 26, 2024
    Inventor: Robert Sackstein
  • Patent number: 11926819
    Abstract: Provided are methods of adding a polymer of non-canonical nucleotides to the 3? end of a ribonucleic acid (RNA). In certain embodiments, the methods comprise combining an RNA, a polynucleotide-3? nucleotidyl transferase, and non-canonical nucleotides, in a reaction mixture under conditions in which the polynucleotide-3? nucleotidyl transferase adds a polymer of the non-canonical nucleotides to the 3? end of the RNA. Such methods may further include analyzing the RNA using a nanopore. According to some embodiments, the methods include identifying the polymer of non-canonical nucleotides added to the 3? end of the RNA, and determining the junction between the 3? end of the RNA and the polymer of non-canonical nucleotides to identify the 3? end of the RNA. Kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
    Type: Grant
    Filed: May 28, 2020
    Date of Patent: March 12, 2024
    Assignee: The Regents of the University of California
    Inventors: Manuel Ares, Logan Mulroney, Jenny Vo, Mark Akeson
  • Patent number: 11913033
    Abstract: The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications.
    Type: Grant
    Filed: December 6, 2017
    Date of Patent: February 27, 2024
    Assignee: Light Bio, Inc.
    Inventor: Il'ya Viktorovich Yampol'skiy
  • Patent number: 11905541
    Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using an endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.
    Type: Grant
    Filed: June 10, 2022
    Date of Patent: February 20, 2024
    Assignee: DNA Script SAS
    Inventor: Sandrine Creton
  • Patent number: 11899021
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: January 4, 2023
    Date of Patent: February 13, 2024
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi