Patents Examined by Maryam Monshipouri
  • Patent number: 10655156
    Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.
    Type: Grant
    Filed: January 29, 2019
    Date of Patent: May 19, 2020
    Assignee: Amgen Inc.
    Inventors: Shivani Gupta, Sohye Kang
  • Patent number: 10648043
    Abstract: The present invention relates to a method for producing fatty acids, in particular shot/medium-chain fatty acids (S/MCFA), by expression in a host cell of a recombinant fatty acid synthase (FAS) naturally devoid of a thioesterase (TE) domain comprising a heterologous thioesterase (TE). The invention further relates to polynucleotides comprising such recombinant FAS, polypeptides encoded thereby, and vectors comprising such polynucleotides, as well as recombinant host cells comprising the polynucleotides, polypeptides, or vectors.
    Type: Grant
    Filed: February 20, 2017
    Date of Patent: May 12, 2020
    Assignee: TOTAL RAFFINAGE CHIMIE
    Inventors: Zhiwei Zhu, Anastasia Krivoruchko, Jens Nielsen
  • Patent number: 10646555
    Abstract: Processes and compositions for the therapeutic treatment of pathogenic Gram-negative bacterial infection are provided whereby argininosuccinate synthetase or PEGylated argininosuccinate synthetase is administered to a subject to inactivate endotoxin thereby reducing the likelihood of bacterial sepsis and improving patient outcome.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: May 12, 2020
    Assignee: BioRegency, Inc.
    Inventors: Stanislav I. Svetlov, Victor Prima, Alvin Wang, Gabriel Molina, Kevin Ka-wang Wang
  • Patent number: 10640538
    Abstract: A recombinant dietary protein is provided that is free of phenylalanine and is a dietary protein with a high biological value. Further provided are a vector encoding said dietary protein, a microorganisms expressing said protein, a method for the production of said protein, and a dietary composition comprising said protein that in an embodiment is for use as a medicament and/or food for special medical purposes in patients with accumulations of phenylalanine in the body.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: May 5, 2020
    Assignee: METAX INSTITUT FÜR DIÄTETIK GMBH
    Inventors: Bernhard Hoffmann, Yvonne Mücke, Stefan Rasche, Natalia Jablonka, Stefan Schillberg
  • Patent number: 10639357
    Abstract: Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.
    Type: Grant
    Filed: September 3, 2019
    Date of Patent: May 5, 2020
    Assignee: The Regents of the University of California
    Inventors: Daniel Garrido, J. Bruce German, Carlito B. Lebrilla, David A. Mills
  • Patent number: 10640740
    Abstract: The present invention is directed to a method for decreasing the lag time in a fermentation of a culture medium to prepare a target substance, wherein the target substance is not yogurt and wherein the fermentation results in formation of acid or ethanol, which method comprises the steps of providing, to a suitable culture medium, a fermentation starter culture comprising a microorganism which liberates acid or ethanol, adding a potato protein protease inhibitor to the culture medium, culturing the microorganism, and obtaining the target substance. It has been found that addition of potato protein protease inhibitor to a fermentation feed significantly reduces the lag time of the fermentation. The required amount of potato protein is low enough not to affect the taste of the target substance adversely, and the lag time reduction occurs both in batch- and in semi-continuous processes. The invention further pertains to a fermented food product which is not yogurt, comprising potato protein protease inhibitor.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: May 5, 2020
    Assignee: COOPERATIVE AVEBE U.A.
    Inventors: Marco Luigi Federico Giuseppin, Catharina Maria Antoinette Mooij, Robin Eric Jacobus Spelbrink
  • Patent number: 10633454
    Abstract: The present invention provides recombinant cells that contain a genetic modification to at least one mannosyl transferase gene. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or more easily humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a reference cell. In some embodiments the modification is a deletion or disruption of a mannosyl transferase gene, which can be in an alg3 gene. Therefore, the proteins produced are more useful for the production of therapeutic glycoproteins than those produced by species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: April 28, 2020
    Assignee: Conagen Inc.
    Inventor: Nicky C. Caiazza
  • Patent number: 10633679
    Abstract: The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having 55% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: April 28, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Su Jin Park, Hee Kyoung Jung, Young Lyeol Yang, Hong Xian Li, Hye Won Um
  • Patent number: 10626425
    Abstract: The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having 55% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same.
    Type: Grant
    Filed: January 4, 2019
    Date of Patent: April 21, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Su Jin Park, Hee Kyoung Jung, Young Lyeol Yang, Hong Xian Li, Hye Won Um
  • Patent number: 10626427
    Abstract: The present invention relates to a mutated protein of FMO derived from Celeribacter sp. and a gene encoding the same, a vector comprising the gene, a recombinant cell transformed by the vector, a composition for producing indigo comprising them, and a method for increasing indigo production in the recombinant cell using the transformed recombinant cell.
    Type: Grant
    Filed: April 22, 2019
    Date of Patent: April 21, 2020
    Assignee: GYEONGBUK INSTITUTE FOR MARINE BIOINDUSTRY
    Inventors: Jung Hee Woo, Hae Seon Kim, Nyun-Ho Park
  • Patent number: 10626381
    Abstract: The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: April 21, 2020
    Assignee: ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S. A.
    Inventors: Francisco Manuel Reyes Sosa, Bruno Díez García, Noelia Valbuena Crespo, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Ana Isabel Platero Gómez, Lucía Martín Pérez, Sandra Gavaldá Martín, Laura Viñas De La Cruz, Laura Sánchez Zamorano, Consolación Álvarez Núñez, María de los Angeles Bermúdez Alcántara, Javier Rocha Martín, Laura Ledesma García, Juan Luis Ramos Martín
  • Patent number: 10619141
    Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: May 29, 2019
    Date of Patent: April 14, 2020
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Signe E. Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoee-Nielsen, Randy Deinhammer, Xinyu Shen
  • Patent number: 10619147
    Abstract: There is provided chimeric polypeptides capable of converting xylose to xylulose, engineered host cells that express the chimeric polypeptides, methods of creating chimeric polypeptides, and methods of fermenting cellulosic biomass to produce biofuels, including ethanol.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: April 14, 2020
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: Allan Froehlich, Brooks Henningsen
  • Patent number: 10612009
    Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: April 7, 2020
    Assignees: Roche Sequencing Solutions, Inc., Roche Molecular Systems, Inc.
    Inventors: Aruna Ayer, Cleoma Renetta Arnold, Arkadiusz Bibillo, Mara Boenitz-Dulat, Barbara Eckert, Ilya Lederman, Colin Alexander McGaw, Charles Wayan Schwab, Tyler O'Brien Shultz, Shawn Suko, Eileen Thai, Bigna Woersdoerfer, David Daniel Wunderlich
  • Patent number: 10604774
    Abstract: Provided herein are processes and microorganisms which utilize both protein hydrolysates and carbohydrates from biomass feedstocks to produce renewable hydrocarbon compositions. Advantages of the disclosed methods may be recognized in fuel blends comprising such hydrocarbon compositions.
    Type: Grant
    Filed: October 31, 2017
    Date of Patent: March 31, 2020
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Larry Cameron Anthony, Tyler T. Ames, Keith H. Burlew
  • Patent number: 10597647
    Abstract: Provided herein in some embodiments is a non-naturally occurring variant of a wild type restriction enzyme defined by SEQ ID NO: 20, wherein the variant has at least a 2 fold increase in cleavage at 5-? glucosylhydroxymethylcytosine (5?ghmC) compared with methylcytosine relative to the wild type enzyme. Methods for examining hydroxymethylation of a DNA sample using the variant enzyme are also provided.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: March 24, 2020
    Assignee: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Janine Borgaro, Aine Quimby, Shengxi Guan, Zhiyi Sun
  • Patent number: 10597612
    Abstract: Cleaning compositions having an enzyme system, where the enzyme system includes a nuclease enzyme, an extracellular-polymer-degrading enzyme, and a cleaning adjunct. Methods of making and using such cleaning compositions. Use of an extracellular-polymer-degrading enzyme.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: March 24, 2020
    Assignee: The Procter & Gamble Company
    Inventors: Neil Joseph Lant, Montserrat Guadalupe Vasquez Valdivieso
  • Patent number: 10597646
    Abstract: An acyl-ACP thioesterase consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: March 24, 2020
    Assignee: Kao Corporation
    Inventors: Tatsuro Ozaki, Hiroyuki Ohta, Koichi Hori
  • Patent number: 10590450
    Abstract: Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: March 17, 2020
    Assignee: DuPont Industrial Biosciences USA, LLC
    Inventors: Mark S. Payne, Yefim Brun, Hongxian He, Thomas Scholz
  • Patent number: 10590428
    Abstract: Provided is a microorganism having high sophorolipid production capability. Disclosed is a sophorolipid-producing yeast mutant strain, in which a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:2 or an amino acid sequence having at least 90% identity therewith, has been deleted or deactivated.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: March 17, 2020
    Assignee: Kao Corporation
    Inventors: Takahiro Ichihara, Yasushi Kageyama, Masatoshi Tohata, Fumikazu Takahashi