Patents Examined by Maryam Monshipouri
  • Patent number: 11028418
    Abstract: Recombinant microorganisms configured for enhanced production of compounds such as 2-pyrone-4,6-dicarboxylic acid (PDC) and methods of using the recombinant microorganisms for the production of these compounds. The recombinant microorganisms include one or more modifications that reduce 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) cis-trans isomerase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) methyl esterase activity, and/or vanillate/3-O-methylgallate O-demethylase activity. The recombinant microorganisms can be used to generate PDC from media comprising plant-derived phenolics, such as syringyl phenolics, guaiacyl phenolics, and p-hydroxyphenyl phenolics. The plant-derived phenolics can be derived from pretreated lignin, including depolymerized lignin or other chemically altered lignin.
    Type: Grant
    Filed: February 19, 2020
    Date of Patent: June 8, 2021
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Daniel R. Noguera, Timothy James Donohue, Jose Perez, Wayne S. Kontur, German Eduardo Umana Chapeton
  • Patent number: 11028415
    Abstract: A process for the production of lipids from biomass derived from guayule plants comprising: obtaining a hydrolysate comprising 5 carbon atom (C5) sugars from biomass derived from guayule plants, said 5 carbon atom (C5) sugars being present in said hydrolysate in a quantity greater than or equal to 80% by weight, preferably ranging from 85% by weight to 99% by weight, with respect to the total weight of said hydrolysate; feeding said hydrolysate to a fermentation device in the presence of at least one oleaginous yeast to obtain a fermentation broth; at the end of fermentation, subjecting said fermentation broth to separation obtaining an aqueous suspension of oleaginous cellular biomass comprising lipids and an aqueous phase. The lipids thus obtained can be advantageously used in the production of biofuel such as, for example, biodiesel or green diesel that can be used as such, or in mixtures with other fuels, for automotive transport.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: June 8, 2021
    Assignees: Versalis S.P.A., Eni S.P.A.
    Inventors: Daniela Cucchetti, Ezio Battistel, Chiara Gambaro, Alessandra D'Arminio Monforte
  • Patent number: 11008565
    Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: May 18, 2021
    Assignee: SEATTLE CHILDREN'S RESEARCH INSTITUTE
    Inventors: Andrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
  • Patent number: 11000576
    Abstract: Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: May 11, 2021
    Assignee: The Regents of the University of California
    Inventors: Daniel Garrido, J. Bruce German, Garlito B. Lebrilla, David A. Mills
  • Patent number: 10995332
    Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: May 4, 2021
    Assignee: SEATTLE CHILDREN'S RESEARCH INSTITUTE
    Inventors: Andrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
  • Patent number: 10988789
    Abstract: Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
    Type: Grant
    Filed: February 14, 2020
    Date of Patent: April 27, 2021
    Assignee: NUTRITION & BIOSCIENCES USA 4, INC.
    Inventors: Mark S. Payne, Yefim Brun, Hongxian He, Thomas Scholz
  • Patent number: 10988740
    Abstract: Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: April 27, 2021
    Inventor: Svetlana Oard
  • Patent number: 10988791
    Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.
    Type: Grant
    Filed: July 20, 2020
    Date of Patent: April 27, 2021
    Assignee: GLYKOS FINLAND OY
    Inventors: Christopher Landowski, Anne Huuskonen, Ann Westerholm-Parvinen, Markku Saloheimo, Anne Kanerva, Jukka Hiltunen
  • Patent number: 10988784
    Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: April 27, 2021
    Assignee: WHITE DOG LABS, INC.
    Inventors: Bryan Patrick Tracy, Shawn William Jones, Daniel Knox Mitchell, Aharon M. Eyal, Karthikeyan Ramachandriya
  • Patent number: 10988715
    Abstract: A method of treating cotton comprising: (i) contacting cotton with an aqueous wash liquor comprising water and an endo-?-1,3-glucanase enzyme; (ii) optionally rinsing the cotton; and (iii) drying the cotton. The wash liquor may contain surfactant. The cotton may be contacted with the aqueous wash liquor with agitation. The method may be for removal of callose from cotton. The method may be for the manufacture of a cotton-containing fabric having improved whiteness maintenance, soil repellency, reduced malodour, anti-wrinkle benefits, and/or improved drying.
    Type: Grant
    Filed: March 12, 2020
    Date of Patent: April 27, 2021
    Assignee: The Procter & Gamble Company
    Inventor: Neil Joseph Lant
  • Patent number: 10982199
    Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: April 20, 2021
    Assignee: BASF Enzymes LLC
    Inventors: Xuqiu Tan, Arne I. Solbak, Jr.
  • Patent number: 10973888
    Abstract: The present invention provides engineered human alpha-galactosidase polypeptides and compositions thereof. The engineered human alpha-galactosidase polypeptides have been optimized to provide improved stability under both acidic (pH<4.5) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered human alpha-galactosidase polypeptides for therapeutic purposes.
    Type: Grant
    Filed: August 5, 2020
    Date of Patent: April 13, 2021
    Assignee: Codexis, Inc.
    Inventors: Nicholas J. Agard, Mathew G. Miller, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 10968438
    Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
    Type: Grant
    Filed: November 25, 2019
    Date of Patent: April 6, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Aruna Ayer, Cleoma Arnold, Charles Wayan Schwab, Eileen Thai, Ilya Lederman, Colin Alexander McGaw, Tyler O'Brien Shultz
  • Patent number: 10968439
    Abstract: The present invention relates to the technical field of protein engineering, and, in particular, to a xylanase mutant with improved heat tolerance. The present invention artificially introduces two or three unnatural disulfide bridges into xylanase by site-directed mutagenesis and obtains the mutants XynA1 and XynA2 with improved heat tolerance, especially the mutant XynA2 into which three disulfide bridges are introduced, achieving residual enzyme activity of 75% after treatment at 80° C. for 5 min, 72% higher than the residual enzyme activity of the mutant XynA1. The present invention further obtains, by screening, three mutation sites Q51N, H143K, and Q161F that can significantly increase the heat tolerance of mutants, and introduction of the mutation sites into the XynA1 and XynA2 xylanases, whether at a single point or a combination of two points or a combination of three points, can effectively improve the heat tolerance of the mutants.
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: April 6, 2021
    Assignee: QINGDAO VLAND BIOTECH GROUP CO., LTD
    Inventors: Xiuxiu Wu, Chao Shao, Yijun Huang
  • Patent number: 10961520
    Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: March 30, 2021
    Assignees: Roche Molecular Systems, Inc., Roche Sequencing Solutions, Inc.
    Inventors: Aruna Ayer, Cleoma Renetta Arnold, Arkadiusz Bibillo, Mara Boenitz-Dulat, Barbara Eckert, Ilya Lederman, Colin Alexander McGaw, Charles Wayan Schwab, Tyler O'Brien Shultz, Shawn Suko, Eileen Thai, Bigna Woersdoerfer, David Daniel Wunderlich
  • Patent number: 10954533
    Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: March 23, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
  • Patent number: 10954494
    Abstract: The invention relates to novel 7ß-hydroxysteroid dehydrogenase mutants, to the sequences which encode these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCS). The invention also relates to novel processes for the synthesis of UDCS using the enzyme mutants; and to the preparation of UDCS using recombinant, multiply-modified microorganisms.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: March 23, 2021
    Assignee: Pharmazell GmbH
    Inventors: Dirk Weuster-Botz, Michael Braun, Arno Aigner, Boqiao Sun, Christina Kantzow, Sven Bresch, Daniel Bakonyi, Werner Hummel
  • Patent number: 10947529
    Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: March 16, 2021
    Assignee: SEATTLE CHILDREN'S RESEARCH INSTITUTE
    Inventors: Andrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
  • Patent number: 10947517
    Abstract: Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.
    Type: Grant
    Filed: February 13, 2020
    Date of Patent: March 16, 2021
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Fuqiang Chen
  • Patent number: 10947523
    Abstract: A biotechnological production of tryptophan and derivatives thereof, as well as a method for an enhanced microbial L-tryptophan synthesis. In one aspect the invention provides a bacterial cell being genetically modified to express anindole-3-glycerol phosphate synthase, IGPs, the IGPs being less sensitive to inhibition or even being activated by anthranilate compared to the wild type IGPs of the bacterial cell.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: March 16, 2021
    Assignee: Technische Universitaet Hamburg
    Inventors: An-Ping Zeng, Lin Chen