Patents Examined by Maryam Monshipouri
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Patent number: 11708566Abstract: Recombinant DP04-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DP04 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: GrantFiled: May 3, 2018Date of Patent: July 25, 2023Assignee: Stratos Genomics, Inc.Inventors: Mark Stamatios Kokoris, Marc Prindle, Jack Chase, Robert Busam, Michael Kovarik, Salka Keller, Megan Murt, Greg Thiessen
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Patent number: 11701386Abstract: The present invention relates to a cell comprising a chimeric antigen receptor (CAR) and a constitutively active or inducible Signal Transducer and Activator of Transcription (STAT) molecule.Type: GrantFiled: September 12, 2018Date of Patent: July 18, 2023Assignee: AUTOLUS LIMITEDInventors: Martin Pulé, Shaun Cordoba, Simon Thomas, Shimobi Onuoha, Matteo Righi
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Patent number: 11696596Abstract: The present invention relates to a process for forming a functionalised dietary fibre comprising admixing an enzyme and an aqueous suspension of dietary fibre, wherein said dietary fibre is at a D50 particle size distribution of less than 30 microns after degradation by the enzyme and comprises less than 25 wt. % soluble fibres and at least 40% wt. % cellulose; denaturing said enzyme to form a functionalised, amphiphilic dietary fibre with adsorbed enzyme. The present invention further relates to a Pickering particle comprising a functionalised dietary fibre and denatured enzyme and the use of the functionalised dietary fibre and denatured enzyme according to present invention or the Pickering particle according to the present invention to stabilize an emulsion.Type: GrantFiled: December 19, 2018Date of Patent: July 11, 2023Assignee: Societe des Produits Nestle S.A.Inventors: Isabel Fernandez Farres, Zeynel Deniz Gunes, Christina Vafeiadi, Lionel Jean Rene Bovetto, Anna Mosior
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Patent number: 11697817Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.Type: GrantFiled: June 25, 2018Date of Patent: July 11, 2023Assignees: UTILIZATION OF CARBON DIOXIDE INSTITUTE CO., LTD., THE UNIVERSITY OF TOKYOInventors: Hideaki Yukawa, Naoto Ohtani, Masaharu Ishii
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Patent number: 11692159Abstract: The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.Type: GrantFiled: January 11, 2021Date of Patent: July 4, 2023Assignee: ALDEVRON, LLCInventor: Isabelle Louise Chambers
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Patent number: 11692187Abstract: The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.Type: GrantFiled: December 21, 2017Date of Patent: July 4, 2023Assignees: VIB vzw, Katholieke Universiteit Leuven, K.U. Leuven R&D, GlobalYeast N.V.Inventors: Johan Thevelein, Mekonnen Demeke, Maria Remedios Foulquié Moreno, Stijn De Graeve, Edgard Valdomiro Charles Belo
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Patent number: 11667902Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.Type: GrantFiled: December 2, 2020Date of Patent: June 6, 2023Assignee: DSM IP ASSETS B.V.Inventors: Rene Marcel De Jong, Chantal Christis, Neil Carr
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Patent number: 11661590Abstract: Some aspects of this disclosure provide a fusion protein comprising a guide nucleotide sequence-programmable DNA binding protein domain (e.g., a nuclease-inactive variant of Cas9 such as dCas9), an optional linker, and a recombinase catalytic domain (e.g., a tyrosine recombinase catalytic domain or a serine recombinase catalytic domain such as a Gin recombinase catalytic domain). This fusion protein can recombine DNA sites containing a minimal recombinase core site flanked by guide RNA-specified sequences. The instant disclosure represents a step toward programmable, scarless genome editing in unmodified cells that is independent of endogenous cellular machinery or cell state.Type: GrantFiled: August 9, 2017Date of Patent: May 30, 2023Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Brian Chaikind, Jeffrey L. Bessen
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Patent number: 11662349Abstract: The present invention relates to sensors and methods for detecting carbohydrates, such as lactose, in a sample. The sensors and methods may also be used to determine the amount of carbohydrate in the sample.Type: GrantFiled: August 7, 2018Date of Patent: May 30, 2023Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Karine Caron, Stephen Charles Trowell
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Patent number: 11655485Abstract: The invention relates to a process for the production of ethanol comprising fermenting a (optionally liquefied) corn slurry under anaerobic conditions in the presence of a recombinant yeast; and recovering the ethanol, wherein said recombinant yeast functionally expresses a heterologous nucleic acid sequence encoding a glucoamylase having an amino acid sequence according to SEQ ID NO: 1 or which glucoamylase is a functional homologue thereof having a sequence identity of at least 80%, or which glucoamylase is a functional homologue which is derived, by way of one or more amino acid substitutions, deletions or insertions, from the amino acid sequence of SEQ ID NO: 1, and wherein the process comprises dosing a glucoamylase at a concentration of 0.05 g/L or less.Type: GrantFiled: August 15, 2019Date of Patent: May 23, 2023Assignee: DSM IP ASSETS B.V.Inventor: Hans Marinus Charles Johannes De Bruijn
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Patent number: 11655463Abstract: A modified endoinulinase is provided, comprising modified wild-type T. purpuregenus endoinulinase, or a functional fragment thereof, in which an amino acid residue at each one of one or more positions corresponding to 128, 316, 344, 350 or 504 of wild-type T. purpuregenus endoinulinase is substituted, wherein: (i) a tyrosine residue corresponding to Y128 is substituted with H, K or R; a glutamate residue corresponding to E344 is substituted with K, H or R; and a threonine residue corresponding to T504 is substituted with M, S or Y; and optionally an alanine residue corresponding to A316 is substituted with T, S, C or M; (ii) a tyrosine residue corresponding to Y128 is substituted with H, K or R; a glutamate residue corresponding to E344 is substituted with K, H or R; a threonine residue corresponding to T504 is substituted with M, S or Y; and a glutamine residue corresponding to Q350 is substituted with L, G, A, V or I; or (iii) a tyrosine residue corresponding to Y128 is substituted with H, K or R.Type: GrantFiled: January 31, 2021Date of Patent: May 23, 2023Assignee: MIGAL GALILEE RESEARCH INSTITUTE LTD.Inventors: Itamar Yadid, Rami Cohen, Livnat Afriat-Jurnou
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Patent number: 11655492Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.Type: GrantFiled: June 2, 2021Date of Patent: May 23, 2023Assignee: R.P. Scherer Technologies, LLCInventors: David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
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Patent number: 11639515Abstract: The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in P. pastoris. On this basis, optimization of the medium and culture conditions of recombinant P. pastoris can significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%.Type: GrantFiled: May 13, 2022Date of Patent: May 2, 2023Assignees: TAIXING DONGSHENG BIO-TECH CO., LTD, JIANGNAN UNIVERSITYInventors: Xinrui Zhao, Bohan Zhang, Jingwen Zhou, Guocheng Du, Jianghua Li, Jian Chen, Fei Yu, Wei Lu, Yuan Qian
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Patent number: 11633461Abstract: Provided herein are deglycosylating enzymes that remove a broad range of N-glycans from N-glycosylated proteins. Further provided are methods of recombinantly producing and expressing the deglycosylating enzymes. The presently described deglycosylating enzymes can be used to produce free glycans for characterization, and for prebiotic and immunostimulatory uses. In addition, the presently described deglycosylating enzymes can be used to produce deglycosylated proteins for characterization, to improve digestion, and to reduce immunogenicity.Type: GrantFiled: April 16, 2021Date of Patent: April 25, 2023Assignee: The Regents of the University of CaliforniaInventors: Daniel Garrido, J. Bruce German, Carlito B. Lebrilla, David A. Mills
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Patent number: 11634740Abstract: The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue; and various uses of the novel allulose epimerase variant. The novel allulose epimerase variant according to the present invention has a higher conversion rate of fructose to allulose compared to that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, and thus can prevent contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shorten production time, and reduce production costs.Type: GrantFiled: April 27, 2021Date of Patent: April 25, 2023Assignee: DAESANG CORPORATIONInventors: Hyung Seop Youn, Eun Seok Choi, Ji Ha Lee, Suok Su Kim, Seung Woo Cho, Tae Yong Kim, Hak Jun Kim
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Patent number: 11634741Abstract: The present invention is related to a method for adding one or more L-nucleotides to the 3?end of a first L-nucleic acid, wherein the method comprises the step of reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising a mutant enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more L-nucleotides to the 3? end of the first L-nucleic acid, wherein the mutant enzymatic activity exhibiting moiety comprises an amino acid sequence, wherein the amino acids of the amino acid sequence are D-amino acids, wherein the mutant enzymatic activity exhibiting moiety is a variant of an enzymatic activity exhibiting moiety, wherein the enzymatic activity exhibiting moiety consists of an amino acid sequence according to SEQ ID NO: 15 and wherein the amino acids of the amino acid sequence according to SEQ ID NO: 15 are D-amino acids, wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety differs from theType: GrantFiled: November 20, 2014Date of Patent: April 25, 2023Assignee: Aptarion biotech AGInventors: Andreas Pech, Florian Jarosch, Michael Jahnz, Sven Klussmann, Ralf David
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Patent number: 11618913Abstract: The present invention relates to a method for preparing oligosaccharides which can be used among others as food additives to reduce calorie content, to sweeten food products, to increase the fiber content of food products, to improve the texture of food products and to stimulate the gut microbiome bacteria. Furthermore they can be applied in the fields of animal feed, or other applications. More particularly, this invention is directed to a high temperature hydrolysis of xyloglucan polysaccharide to defined xyloglucan oligosaccharides. The invention further relates to oligosaccharide hydrolysates produced with the method of the invention and to the use of said oligosaccharide hydrolysates in human and/or animal nutrition, as prebiotic or other uses. Further provided are novel endoglucanases for use in the method of the invention as well as in other applications.Type: GrantFiled: November 16, 2018Date of Patent: April 4, 2023Assignee: TECHNISCHE UNIVERSITÄT MÜNCHENInventors: Sigrid Graubner, Vladimir Zverlov, Wolfgang Schwarz, Petra Eichinger, Björn Andreessen, Jonathan Herlet, Matthias Mechelke, Philipp Schulte, Wolfgang Liebl
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Patent number: 11591588Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.Type: GrantFiled: November 6, 2020Date of Patent: February 28, 2023Assignee: Codexis, Inc.Inventors: Oscar Alvizo, David Elgart, Robert Kevin Orr, James Nicholas Riggins, Anna Fryszkowska, Katrina W. Lexa, Xiang Yi, Da Duan, Courtney Dianne Moffett, Nikki Dellas, Vesna Mitchell
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Patent number: 11584783Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.Type: GrantFiled: June 26, 2018Date of Patent: February 21, 2023Assignee: NOVOZYMES A/SInventors: Marc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Patent number: 11567083Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).Type: GrantFiled: November 25, 2020Date of Patent: January 31, 2023Assignee: Promega CorporationInventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi