Patents Examined by Maryam Monshipouri
  • Protein extraction method without lysing cells
    Patent number: 11459571
    Abstract: A method for extracting protein without lysing cells includes: step 1: combining a penetrating peptide CPP gene having a sequence as set forth in SEQ ID NO: 1 and a bacterial lyase T4L gene having a sequence as set forth in SEQ ID NO: 2 through a flexible connecting peptide GGGGS gene having a sequence as set forth in SEQ ID NO: 3 to form a fusion enzyme CPP-T4L gene having a sequence as set forth in SEQ ID NO: 4; step 2: inserting the fusion enzyme CPP-T4L gene, and obtaining a recombinant host strain; step 3: cloning a target protein expression gene, and then constructing a recombinant expression strain; step 4: first inducing the expression of the target protein expression gene; starting the expression of the fusion enzyme CPP-T4L gene, and releasing a target protein; step 5: collecting cell lysis supernatant to recover the target protein.
    Type: Grant
    Filed: April 13, 2021
    Date of Patent: October 4, 2022
    Assignee: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Jian Zha, Zhiqiang Liu, Guoli Gong, Runcong Sun
  • Ligase fusion proteins and application thereof
    Patent number: 11453870
    Abstract: The present disclosure relates to the field of biotechnology. In particular, provided are a ligase fusion protein and an immobilized ligase comprising the same. Also provided is use of the ligase fusion protein or the immobilized ligase in the preparation of conjugates. Further provided is a process for the preparation of conjugates using a ligase or a ligase unit.
    Type: Grant
    Filed: July 13, 2021
    Date of Patent: September 27, 2022
    Assignee: GENEQUANTUM HEALTHCARE (SUZHOU) CO. LTD.
    Inventors: Gang Qin, Cao Lv, Joachim Walter, Rolf Werner, Yunpeng Zhong
  • Cell-free glycoprotein synthesis (CFGpS) in prokaryotic cell lysates enriched with components for glycosylation
    Patent number: 11453901
    Abstract: Disclosed are components and systems for cell-free glycoprotein synthesis (CFGpS). In particular, the components and systems include and utilize prokaryotic cell lysates from engineered prokaryotic cell strains that have been engineered to enable cell-free synthesis of glycoproteins.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: September 27, 2022
    Assignees: Northwestern University, Cornell University
    Inventors: Michael Christopher Jewett, Jessica Carol Stark, Matthew P. DeLisa, Thapakorn Jaroentomeechai
  • Reduction in acetate production by yeast over-expressing PAB1
    Patent number: 11447783
    Abstract: Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: September 20, 2022
    Assignee: DANISCO US INC.
    Inventors: Min Qi, Paula Johanna Maria Teunissen, Quinn Qun Zhu
  • Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus
    Patent number: 11447530
    Abstract: This disclosure describes compositions and methods for enhanced production of enduracidin in genetically engineered strains of Streptomyces fungicidicus. In particular, the present disclosure describes the genetic manipulation of regulatory genes orf24 and orf18 associated with the enduracidin (enramycin) biosynthesis gene cluster from Streptomyces fungicidicus to generate vector constructs and recombinant strains producing greater yields of enduracidin.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: September 20, 2022
    Assignee: OREGON STATE UNIVERSITY
    Inventors: T. Mark Zabriskie, Xihou Yin
  • Self-sterilizing fabrics incorporating anti-viral cold-active proteases
    Patent number: 11441261
    Abstract: The invention provides fabrics that incorporate protease enzymes that inactivate viruses and bacteria. The fabrics of the invention may be used in the production of various items of self-sterilizing protective equipment including protective facemasks.
    Type: Grant
    Filed: September 6, 2021
    Date of Patent: September 13, 2022
    Assignee: WABESO Enhanced Enzymatics, Inc.
    Inventor: Mandy Jane Ward
  • Methanol dehydrogenase fusion proteins
    Patent number: 11441128
    Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: September 13, 2022
    Assignee: Genomatica, Inc.
    Inventors: Nelson R. Barton, Jingyi Li, Joseph R. Warner, Priti Pharkya
  • Engineered primate cystine/cysteine degrading enzymes as antineogenic agents
    Patent number: 11426464
    Abstract: Methods and compositions related to the engineering of a protein with L-cyst(e)ine degrading enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase comprising one or more amino acid substitutions and capable of degrading L-cyst(e)ine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with L-cyst(e)ine using the disclosed proteins or nucleic acids.
    Type: Grant
    Filed: July 15, 2020
    Date of Patent: August 30, 2022
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: George Georgiou, Everett Stone
  • Process for production of sugar from a cotton-containing textile
    Patent number: 11421257
    Abstract: Cotton-containing textiles, such as “trash” feedstock in terms of end-of-life-cotton textiles, may be used to produce sugar without the same kinds of harsh pretreatments used for other biomasses, such as corn, grass sources, or wood. Disclosed is a process for production of sugar from a cotton-containing textile waste fabric comprising optionally mechanically pretreating the cotton-containing textile, pretreating the cotton-containing textile with an acid pretreatment to form a slurry, cooling the slurry, adding at least one base to the slurry, adding at least one additional acid to the slurry to form a buffer in situ, adding a hydrolysis enzyme, and optionally filtering the slurry.
    Type: Grant
    Filed: June 26, 2020
    Date of Patent: August 23, 2022
    Assignee: Cotton Inc.
    Inventors: Matthew J Farrell, Sha Fu, Mary A Ankeny
  • Performance-enhanced protease variants II
    Patent number: 11421213
    Abstract: An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) amino acid substitutions at the positions corresponding to the positions 9 and 271 in each case based on the numbering according to SEQ ID No. 1, and (b) an amino acid substitution on at least one of the positions corresponding to the positions 29, 48, 101, 30, 131, 133, 144, 224 or 252, in each case based on the numbering according to SEQ ID No. 1. Such proteases are particularly suitable for having an improved cleaning performance.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: August 23, 2022
    Assignee: HENKEL AG & CO. KGAA
    Inventors: Layla Fernandez, Sabine Griemert, Nina Mussmann, Susanne Wieland, Daniela Herbst, Inken Prueser, Christian Degering, Daria Strauss, Thorsten Eggert, Christian Leggewie
  • Rapid approach for detection of bacterial spores
    Patent number: 11421260
    Abstract: Provided is a method for detecting the presence of bacterial spores by measuring adenosine diphosphate (ATP) production over time. Spores are detected by converting adenosine monophosphate (AMP) and adenosine diphosphate (ADP) to ATP.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: August 23, 2022
    Assignee: ECOLAB USA INC.
    Inventors: Wenli Tu, Zhi Chen
  • Acid-alpha glucosidase variants and uses thereof
    Patent number: 11421211
    Abstract: The present invention relates to variants of acid-alpha glucosidase and uses thereof. Said variants are sequence-optimized and/or are linked to a heterogenous signal peptide.
    Type: Grant
    Filed: September 12, 2017
    Date of Patent: August 23, 2022
    Assignees: GENETHON, SORBONNE UNIVERSITÉ, DUKE UNIVERSITY
    Inventors: Federico Mingozzi, Giuseppe Ronzitti, Dwight D. Koeberl, Sang-Oh Han
  • Methods for preparing antibodies with a defined glycosylation pattern
    Patent number: 11414477
    Abstract: The present disclosure relates to a process for preparing antibodies with a defined glycosylation pattern, in particular antibodies with a glycan terminating in an N-acetylglucosamine. The antibodies of the disclosure are suitable for use in a process to conjugate a payload thereto. The disclosure also extends to molecules obtained and obtainable from the process disclosed herein, novel molecules and intermediates, compositions comprising said molecules and uses of the molecules and compositions, particularly in treatment, for example in the treatment of cancer.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: August 16, 2022
    Assignee: MedImmune, LLC
    Inventors: Changshou Gao, Pamela Thompson, Dorin Toader, Nazzareno Dimasi
  • strains having an oxidative cytoplasm
    Patent number: 11407975
    Abstract: This disclosure provides an E. coli strain, which lacks thioredoxin reductase activity encoded by trxB and thioredoxin 1 activity encoded by trxA, and glutathione reductase activity encoded by gor. Said E. coli strain expresses a mutated AhpC protein having glutathione reductase activity and a cytosolic prokaryotic disulfide isomerase. The E. coli strain has an oxidative cytosol and can be used to efficiently produce proteins having disulfide bonds.
    Type: Grant
    Filed: November 7, 2019
    Date of Patent: August 9, 2022
    Assignee: Sutro Biopharma, Inc.
    Inventor: Daniel Groff
  • Mutant thioesterases
    Patent number: 11408014
    Abstract: Mutant thioesterases having enhanced medium chain substrate activity, polynucleotides encoding and configured to express the mutant thioesterases in a transformed host cell, host cells transformed to contain the polynucleotides, and methods of using same.
    Type: Grant
    Filed: October 15, 2020
    Date of Patent: August 9, 2022
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Brian F. Pfleger, Nestor Hernandez-Lozada, Rung-Yi Lai
  • Fusion proteins and methods for site-directed genome editing
    Patent number: 11407990
    Abstract: In some aspects, the present invention provides methods and compositions for modifying target sites within nucleic acid molecules. In some embodiments, the methods comprise using adenosine deaminases that act on RNA (ADARs), and variants thereof, to modify target sites within DNA-RNA hybrid molecules. In other aspects, ADAR2 variant polypeptides as well as fusion proteins comprising an ADAR catalytic domain and a hybrid nucleic acid binding domain are provided, as are methods for use thereof. Methods for preventing and treating genetic disorders are also provided herein.
    Type: Grant
    Filed: May 18, 2020
    Date of Patent: August 9, 2022
    Assignee: The Regents of the University of California
    Inventors: Yuxuan Zheng, Claire Lorenzo, Peter Beal, Andrew Fisher, Leanna Monteleone
  • Expression vector
    Patent number: 11390882
    Abstract: Disclosed herein are recombinant methods of activating expression of one or more biosynthetic gene clusters comprising more than one gene, the method comprising a recombinant DNA expression vector that possess two opposable inducible promoters that drives expression of a biosynthetic gene cluster exogenously from outside of the cluster to produce polyketides or non-ribosomal peptides in a heterologous host.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: July 19, 2022
    Assignee: VARIGEN BIOSCIENCES CORPORATION
    Inventors: Robert Joseph Stankey, David Mead
  • Method for producing inositol derivative
    Patent number: 11384372
    Abstract: A method for producing an inositol derivative includes a step of reacting inositol and dextrin in the presence of cyclodextrin glucanotransferase to generate an inositol derivative in which a sugar is bonded to the inositol, and to obtain a solution containing the inositol derivative and the cyclodextrin glucanotransferase; and a step of removing the cyclodextrin glucanotransferase in the solution using an ultrafiltration membrane, in which a deactivation treatment of the cyclodextrin glucanotransferase in the solution is not performed.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: July 12, 2022
    Assignee: SHOWA DENKO K.K.
    Inventors: Ichiro Fujita, Shinji Yamaki
  • Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors
    Patent number: 11384140
    Abstract: This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.
    Type: Grant
    Filed: May 11, 2017
    Date of Patent: July 12, 2022
    Assignee: Amgen Inc.
    Inventors: Randal Robert Ketchem, Jeffrey T. McGrew, Dina A. Fomina Yadlin
  • Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through cAMP pathways
    Patent number: 11366114
    Abstract: Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.
    Type: Grant
    Filed: November 4, 2014
    Date of Patent: June 21, 2022
    Assignee: Montana Molecular LLC
    Inventors: Thomas E. Hughes, Paul H. Tewson, Anne Marie Quinn