Patents Examined by Melanie Yu Brown
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Patent number: 10209252Abstract: A substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure and a functional layer attached to the interlayer, the functional layer having functional sites with a density of at least 50 nanomoles/cm2.Type: GrantFiled: March 1, 2011Date of Patent: February 19, 2019Assignee: W.L. Gore & Associates, Inc.Inventors: Anit Dutta, Heidi Flannery, William P. Mortimer, Jr.
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Patent number: 10210307Abstract: Provided are embodiments of a method for determining a serum protein biomarker profile of a subject patient comprising: wicking blood from a subject onto a fluid sample collecting comb consisting of absorbent strips, each absorbent strip consisting of a fibrous absorbent wick configured to absorb a predetermined volume of blood; drying the blood samples on the wicks and eluting serum proteins into an elution buffer; determining the identities and levels of the extracted proteins by microarray analysis; comparing by computer the identities and levels of the extracted proteins with a reference database generated from the blood samples from a plurality of subjects collected by a fluid sample collecting comb and producing a computer-generated report of the identities and levels of the biomarkers of the subject and adjusting the treatment based on the identities and amounts of the protein biomarkers of the blood sample of the subject.Type: GrantFiled: April 28, 2017Date of Patent: February 19, 2019Assignees: RayBiotech, Inc. Guangzhou, RayBiotech Life, Inc., USAInventor: Ruo-Pan Huang
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Patent number: 10197565Abstract: A piezoelectric plate sensor comprising a piezoelectric layer; two electrodes; and an insulation layer. The insulation layer is produced by soaking the piezoelectric layer and two electrodes in a mercaptopropyltrimethoxysilane solution with an amount of water from 0.1 v/v. % to about 1 v/v % and at pH from about 8 to about 150 for a period from about 8 to about 15 hours, and the mercaptopropyltrimethoxysilane solution has a concentration of mercaptopropyltrimethoxysilane from about 0.01 v/v % to about 0.5 v/v %. A method of detecting a biomolecule in a sample using the piezoelectric plate sensor in particular, that of detecting a genetic marker with PCR sensitivity and specificity without the need of DNA isolation or amplification is also provided. The piezoelectric plate sensor may be used to diagnose various diseases including breast cancer, myocardial infarction, diarrhea, Clostridium difficile infection, and hepatitis B infection.Type: GrantFiled: December 19, 2014Date of Patent: February 5, 2019Assignee: DREXEL UNIVERSITYInventors: Wan Y. Shih, Wei Wu, Wei-Heng Shih, Mehmet Cagri Soylu, Haitao Guo, Suresh G. Joshi, Ceyhun Ekrim Kirimli, Ying-Hsiu Su
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Patent number: 10191054Abstract: A device for full blood count includes first channel and second channels separated from each other. The device further includes a first inlet configured to provide a whole blood sample to the first and second channels, a second inlet configured to provide a lysis agent for white blood cell count in to the first channel, a third inlet configured to provide a quench solution to the first channel, and a fourth inlet configured to provide a lysis agent for hemoglobin measurement to the second channel.Type: GrantFiled: January 26, 2010Date of Patent: January 29, 2019Assignee: KONINKLIJKE PHILIPS N.V.Inventors: David M. Pettigrew, James D. Gwyer
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Patent number: 10190986Abstract: A method for analyzing the results of a ligand-receptor binding assay comprising the steps of: (a) providing the results of a ligand-receptor binding assay; and (b) qualifying the results of a ligand-receptor binding assay. More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.Type: GrantFiled: June 6, 2011Date of Patent: January 29, 2019Assignee: Abbott LaboratoriesInventors: Qiaoqiao Ruan, Sylvia C. Saldana, Joseph P. Skinner, Sergey Y. Tetin
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Patent number: 10175246Abstract: A method of detecting a target within a population of molecules comprising: contacting a plurality of labeled probe molecules with the population of molecules potentially containing a target of the probe molecules; acquiring a probe specific signal emitted by said labeled probe molecules that bound to said target together with a background signal; preferentially modulating said probe specific signal by at least one of modulating said acquisition and modulating an emission of said probe specific signal; and detecting said probe specific signal over said background signal using said preferential modulation.Type: GrantFiled: January 31, 2017Date of Patent: January 8, 2019Assignee: Ramot at Tel-Aviv University Ltd.Inventors: Ady Arie, Amos Danielli
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Patent number: 10173216Abstract: A particle capture system that can be used in the context of a lab-on-a-chip platform for particle- and cell-based assays is described. The system comprises a capture chamber comprising a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber. By rotating the chamber, the individual particles are biased towards the capture sites where they may be captured.Type: GrantFiled: July 18, 2014Date of Patent: January 8, 2019Assignee: DUBLIN CITY UNIVERSITYInventors: Jens Ducree, Robert Burger
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Patent number: 10151750Abstract: A detection method (200) detects analytes in a fluid sample. The detection method includes transporting magnetic and/or electric labels (5) after interaction between the sample fluid and a reagent towards a detection receptacle (1). The detection receptacle (1) is initially substantially magnetic and/or electric label (5) free. By transporting the magnetic and/or electric labels (5) after reaction, interference between unreacted reagents and magnetic and/or electric label-assisted detection can be reduced.Type: GrantFiled: December 15, 2014Date of Patent: December 11, 2018Assignee: KONINKLIJKE PHILIPS N.V.Inventors: Wendy Uyen Dittmer, Menno Willem Jose Prims
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Patent number: 10145851Abstract: Methods are provided for the analysis, including the serial analysis, of very small samples of tissue. The methods utilize a nanofluidic proteomic immunoassay (NIA) to quantify total and low-abundance protein isoforms in a small amount of lysate. NIA detection accurately measure oncoprotein expression and activation in limited clinical specimens, including isoforms that differ in post-translational modifications, such as phosphorylation, and the like. The NIA detection method combines isoelectric protein focusing and antibody detection in a nanofluidic system.Type: GrantFiled: January 22, 2016Date of Patent: December 4, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Dean W. Felsher, Alice Fan
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Patent number: 10145846Abstract: A sensing device is provided that includes a tunnel junction created by forming a hole in a layered tunnel junction (for example). A chemically, well-defined surface may be formed by coupling affinity reagents to the electrodes, which, by these means, the surface may be configured to be selective for a particular analyte.Type: GrantFiled: April 16, 2015Date of Patent: December 4, 2018Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Stuart Lindsay, Peiming Zhang, Pei Pang
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Patent number: 10145844Abstract: Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.Type: GrantFiled: June 22, 2016Date of Patent: December 4, 2018Assignee: The University of ToledoInventors: Brent D. Cameron, Dong-Shik Kim
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Patent number: 10126294Abstract: In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field.Type: GrantFiled: May 14, 2015Date of Patent: November 13, 2018Assignee: Abbott Point of Car Inc.Inventor: Cary James Miller
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Patent number: 10126218Abstract: Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.Type: GrantFiled: September 2, 2015Date of Patent: November 13, 2018Assignee: The General Hospital CorporationInventors: Mehmet Toner, Shannon Stott, Chia-Hsien Hsu
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Patent number: 10126296Abstract: The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.Type: GrantFiled: August 5, 2011Date of Patent: November 13, 2018Assignee: Abbott Point of Care Inc.Inventor: Cary James Miller
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Patent number: 10119964Abstract: A device termed the MobileArray™ device and methods, which form the MobileArray™ system, are disclosed for performing multiplex assays, target enrichment or purification. The device and methods disclosed enable the performing of multiplex assays, target enrichment or purification in a simplified manner. The MobileArray™ device gains the advantages and applications of the advanced multiplexing platforms, but does not require the special expensive equipment, reagents, software or dedicated operators. In addition, the MobileArray™ system can also be utilized in immunoprecipitation and target enrichment or purification in a multiplex manner. Furthermore, the MobileArray™ system can be integrated in an automated procedure. The MobileArray™ system makes it possible to apply multiplexing protocols in routine clinical practice, food safety inspection and general life science research laboratories.Type: GrantFiled: September 9, 2015Date of Patent: November 6, 2018Assignee: Digital Biotech, LLCInventor: Raymond Lesser
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Patent number: 10107780Abstract: Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.Type: GrantFiled: March 11, 2015Date of Patent: October 23, 2018Assignee: Bio-Rad Laboratories, Inc.Inventor: William Strong
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Patent number: 10107725Abstract: A method including: providing a sample with M components to be labeled, where M>2; labeling the components with N stains, where N<M so that at least two components are labeled with a common stain; obtaining a set of spectral images of the sample; classifying different parts of the sample into respective classes that distinguish the commonly stained components based on the set of spectral images; and determining relative amounts of multiple ones of the M components in different regions of the sample. Related apparatus are also disclosed.Type: GrantFiled: January 30, 2012Date of Patent: October 23, 2018Assignee: Cambridge Research & Instrumentation, Inc.Inventor: Clifford C. Hoyt
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Patent number: 10107829Abstract: The present invention is to present an analyzing method for analyzing a target substance contained in a sample using a reagent containing magnetic particles. The method comprises steps of: (a) magnetically capturing magnetic particles in a container with a first magnetic force generating member arranged on a lower side of the container accommodating a liquid specimen containing the magnetic particles; (b) transferring the container to a target substance separating section after the step (a); and (c) in the target substance separating section, discharging a cleaning liquid into the container and aspirating a liquid in the container while magnetically capturing the magnetic particles in the container with a second magnetic force generating member.Type: GrantFiled: December 9, 2011Date of Patent: October 23, 2018Assignee: Sysmex CorporationInventors: Hiroto Toyoshima, Kazunori Mototsu
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Patent number: 10101299Abstract: Methods for quantitatively determining a binding kinetic parameter of a molecular binding interaction are provided. Aspects of embodiments of the methods include: producing a magnetic sensor device including a magnetic sensor in contact with an assay mixture including a magnetically labeled molecule to produce a detectable molecular binding interaction; obtaining a real-time signal from the magnetic sensor; and quantitatively determining a binding kinetics parameter of the molecular binding interaction from the real-time signal. Also provided are systems and kits configured for use in the methods.Type: GrantFiled: March 11, 2011Date of Patent: October 16, 2018Assignee: The Board of Trustees of the Leland Standford Junior UniversityInventors: Shan X. Wang, Richard S. Gaster, Liang Xu, Shu-Jen Han, Robert Wilson
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Patent number: 10094831Abstract: Disclosed herein are a norovirus detection sensor and an electrochemical sensing method using the sensor. Specifically, in the norovirus detection sensor including a bioreceptor and a signal detector, a three-dimensional gold nanosurface electrode is used as a substrate, and the bioreceptor employs concanavalin A as a sample capture agent immobilized to the substrate and capable of binding to norovirus. Therefore, the norovirus detection sensor has improved sensitivity by employing the three-dimensional gold nanosurface electrode having a wide surface area. In addition, the norovirus detection sensor has effects of reducing manufacturing costs using a non-antibody material, i.e., concanavalin A which is inexpensive and readily available.Type: GrantFiled: December 29, 2014Date of Patent: October 9, 2018Assignees: GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY, KOREA BASIC SCIENCE INSTITUTE, INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITYInventors: Sung Yang, Du-Woon Kim, Joseph Kwon, Sung-A Hong, Jong-Soon Choi, Heemin Lee