Patents Examined by Michael Paul Shimek
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Patent number: 11977057Abstract: A method for characterizing a target peptide through a detection approach such as mass spectrometry is provided, including: introducing at least one guard molecule to mix with the target peptide; and applying the detection approach for the characterization of the target peptide. Each guard molecule is configured to have similar characteristics as the target peptide, yet is still distinguishable therefrom by the detection approach, such as having a mass spectrometry-distinguishable different M/z value compared with the target peptide. The method can be used to characterize a neoantigen peptide through mass spectrometry, upstream of which the method can further include steps for tissue sample preparation, HLA molecules enrichment, elution, clean-up, and purification. Some or all of these steps can be configured to be executed in a substantially automatic manner with little or no manual intervention. A system for implementing the neoantigen analysis method is further provided.Type: GrantFiled: June 13, 2021Date of Patent: May 7, 2024Assignee: COMPLETE OMICS INTERNATIONAL INC.Inventor: Qing Wang
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Patent number: 11959929Abstract: A method of measuring and identifying LSD and its major metabolite O-H-LSD, by obtaining a sample from an individual, and measuring, identifying, and quantifying LSD and O-H-LSD in the sample by performing a LC-MS/MS analysis. A method of treating and monitoring individuals taking LSD, by administering a microdose of LSD, a prodrug of LSD, or an analog of LSD to the individual, monitoring the individual by obtaining a sample from an individual and measuring and identifying the analytes in the sample by performing a LC-MS/MS analysis, and adjusting the microdose based on the amount of LSD quantified in the LC-MS/MS analysis. A method of adjusting dosing of LSD, by administering a microdose of LSD, a prodrug of LSD, or an analog of LSD to the individual, and adjusting the microdose based on blood concentration analytics.Type: GrantFiled: October 20, 2021Date of Patent: April 16, 2024Assignee: Universitätsspital BaselInventors: Matthias E. Liechti, Urs Philipp Duthaler
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Patent number: 11940429Abstract: A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.Type: GrantFiled: May 1, 2019Date of Patent: March 26, 2024Assignee: Waters Technologies CorporationInventors: Jinchuan Yang, Paul Rainville
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Patent number: 11906525Abstract: The invention, in part, includes methods of single molecule protein sequencing that include using weak binding spectra in the amino acid identification.Type: GrantFiled: May 25, 2022Date of Patent: February 20, 2024Assignee: Massachusetts Institute of TechnologyInventors: Edward Boyden, Adam Henry Marblestone, Samuel Gordon Rodriques
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Patent number: 11906510Abstract: Disclosed herein are methods of analyzing a biological sample comprising: separating components of the biological sample via reversed-phase (RP) chromatography to obtain an elute; subjecting the elute to separation via ion-exchange (IEX) chromatography or mixed-mode IEX chromatography; and detecting the separated compounds to determine the components of the biological sample. Also disclosed are devices comprising a reversed-phase (RP) chromatography column in communication with an ion-exchange (IEX) chromatography column or mixed-mode IEX chromatography column, wherein there is no switching valve between the columns.Type: GrantFiled: March 19, 2021Date of Patent: February 20, 2024Assignee: Stanford Junior UniversityInventor: Anthony T. Le
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Patent number: 11906491Abstract: Provided herein is a method for authenticating animal hide gelatins using peptide markers found animal hide gelatin hydrolysates. The method can be used to authenticate animal hide gelatins derived from donkey hide, horse hide, pig hide, and cattle hide.Type: GrantFiled: July 29, 2021Date of Patent: February 20, 2024Assignee: Hong Kong Baptist UniversityInventors: Quanbin Han, Wenjie Wu, Lifeng Li
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Patent number: 11885777Abstract: A method for efficiently purifying and detecting 6-hydroxynobilonine in fresh stems of Dendrobium huoshanense uses a gas chromatography-mass spectrometry (GC-MS) method to detect 6-hydroxynobilonine in Dendrobium huoshanense is, i.e.Type: GrantFiled: June 21, 2023Date of Patent: January 30, 2024Assignee: Anhui Agricultural UniversityInventors: Xianfeng Du, Yuchen Gao, Li Lin, Yaoyao Xia
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Patent number: 11885780Abstract: Provided are methods for detecting chromogranin A by mass spectrometry. In another aspect, provided herein are methods for quantitating chromogranin A by mass spectrometry. In another aspect, provided herein are methods for prognosis of or measuring the size of neuroendocrine tumors by mass spectrometry.Type: GrantFiled: January 24, 2022Date of Patent: January 30, 2024Assignee: Quest Diagnostics Investments LLCInventors: Darren Weber, Michael P. Caufield, Michael McPhaul, Scott Goldman, Nigel Clarke
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Patent number: 11879880Abstract: Machines and methods are for performing fully-automated biological evaluation and chemical analysis. A pretreatment module is used in treatments of enriching, concentrating and purifying a sample to be analyzed. A component separation module is used for carrying out separation of multiple compounds in mother liquor to be analyzed. A monitoring and identifying module is for monitoring and collecting chromatographic signals of the separated liquid effluent in real time, as well as quantitative detection of suspicious compounds. A component collection module is used in operations of collecting, transferring and dissolving, redissolving, and pipetting the separated liquid effluent. A biological evaluation module is for cell culture and detection of cytotoxic effect and toxic targets. A data processing and automated control module is for acquisition, arrangement and analysis of the integrated data.Type: GrantFiled: July 17, 2019Date of Patent: January 23, 2024Assignee: Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesInventors: Guangbo Qu, Qianchi Ma, Yanna Liu, Jianbo Shi, Guibin Jiang
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Patent number: 11857966Abstract: Methods and devices are provided for sample collection and sample separation. In one embodiment, a device is provided for use with a formed component liquid sample, the device comprising at least one sample inlet for receiving said sample; at least a first outlet for outputting only a liquid portion of the formed component liquid sample; at least a second outlet for outputting the formed component liquid sample at least a first material mixed therein.Type: GrantFiled: March 15, 2018Date of Patent: January 2, 2024Assignee: Labrador Diagnostics LLCInventors: Deborah Sloan, Channing Robertson
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Patent number: 11850535Abstract: The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.Type: GrantFiled: December 19, 2022Date of Patent: December 26, 2023Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Dingjiang Liu, Lin Luo, Long Xu
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Patent number: 11852636Abstract: The invention relates to the detection of non-metabolized vitamin D. In a particular aspect, the invention relates to methods for detecting underivatized non-metabolized vitamin D by mass spectrometry.Type: GrantFiled: December 30, 2022Date of Patent: December 26, 2023Assignee: QUEST DIAGNOSTICS INVESTMENTS INCORPORATEDInventors: Brett Holmquist, Nigel Clarke
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Patent number: 11826758Abstract: Disclosed herein are evacuated blood collection tubes comprising protease inhibitor cocktails in liquid form and uses thereof for assessing features associated with the contact system in a subject, including the endogenous level of contact system activation, the endogenous level of a drug that targets a component of contact system during treatment, and/or the immunogenicity of such a drug.Type: GrantFiled: August 23, 2022Date of Patent: November 28, 2023Assignee: Takeda Pharmaceutical Company LimitedInventors: Daniel J. Sexton, Ryan Faucette
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Patent number: 11774414Abstract: A method of determining total organofluorine in a sample comprising PFAS, comprising: providing a solution of PFAS in an organic solvent to obtain extracted PFAS, or extracting a sample with an organic solvent to obtain extracted PFAS; treating the extracted PFAS with a sodium metal dispersion and alcohol to obtain sodium fluoride; and quantifying the amount of the fluoride. Surprisingly, we discovered that the method recovered substantial fluorine from PFAS and significantly higher yields obtained by selection of the appropriate alcohols. The method is selective for organofluorine from inorganic fluorine.Type: GrantFiled: October 5, 2021Date of Patent: October 3, 2023Assignee: Battelle Memorial InstituteInventors: Kavitha Dasu, Chad Cucksey, Franco Pala
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Patent number: 11768184Abstract: A reagent for use in the measurement of hemoglobins by liquid chromatography, the reagent comprising a nonionic surfactant selected from the group consisting of: (i) polyoxyethylene (10) decyl ether; (ii) polyoxyethylene (6) 2-ethylhexyl ether; (iii) polyoxyethylene (9) isodecyl ether; (iv) polyoxyethylene (10) nonyl ether; (v) polyoxyethylene (16) isostearyl ether; (vi) polyoxyethylene (20) behenyl ether; and (vii) polyoxyethylene (20) polyoxypropylene (6) decyltetradecyl ether.Type: GrantFiled: December 8, 2021Date of Patent: September 26, 2023Assignee: ARKRAY, Inc.Inventor: Mari Miyamoto
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Patent number: 11740246Abstract: Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.Type: GrantFiled: July 25, 2022Date of Patent: August 29, 2023Assignee: Regeneron Pharmaceuticals, Inc.Inventor: Shunhai Wang
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Patent number: 11732315Abstract: Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity.Type: GrantFiled: September 28, 2022Date of Patent: August 22, 2023Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Eric Hunt, Gregory Patton, Guoping Ren, Zhiru Li, Andrew Barry, Nicole Nichols, Catherine B. Poole, Harriet M. Strimpel, Ivan R. Correa, Jr., Clotilde Carlow, Esta Slayton, Thomas C. Evans, Jr.
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Patent number: 11733217Abstract: The present invention relates to detection systems for detecting an opioid compound by use of pyrolysis, as well as methods thereof. In particular, the systems are configured to detect the presence of a backbone fragment indicative of a class of opioid compounds, including opioid analogues.Type: GrantFiled: August 4, 2020Date of Patent: August 22, 2023Assignee: National Technology & Engineering Solutions of Sandia, LLCInventors: Matthew W. Moorman, Joshua J. Whiting, Curtis D. Mowry
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Patent number: 11733230Abstract: The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent.Type: GrantFiled: December 31, 2020Date of Patent: August 22, 2023Assignee: JIANGNAN UNIVERSITYInventors: Yuanfa Liu, Junge Song, Yongjiang Xu, Zhaojun Zheng, Chen Cao, Yinghong Zhai
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Patent number: 11724256Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements (including an integrated imaging subsystem); a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including antibody detection, other protein detection, mRNA detection, and/or other applications associated with spatial transcriptomics.Type: GrantFiled: June 2, 2020Date of Patent: August 15, 2023Assignee: Bio-Rad Laboratories, Inc.Inventor: Kalyan Handique