Abstract: A process (200) for fabricating a flex circuit (708, 806, 812, 818 or 824) using a fabrication process without the use of a photomask includes the steps of generating (412) an electronic image (702 or 802) of circuit traces (704 or 804) representing at least a single-sided flex circuit, and selectively thermal transferring (506 or 606) a resin to either a conductively clad or non-conductive flexible substrate (304) under the control of the electronic image (702 or 802) to form either an etch resist or a conductor which defines the circuit traces (704 or 804). The conductively clad flexible substrate (304) is etched to form the circuit traces (704 or 804) of the flex circuit defined by the etch resist, after which the etch resist is removed.

Abstract: A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.

Abstract: A method is provided for preparing monomeric calicheamicin derivative/carrier conjugates with higher drug loading/yield and decreased aggregation. These conjugates are prepared by incubating a calicheamicin derivative and a proteinaceous carrier in a solution comprising a phosphate buffered solution, a cosolvent comprising propylene glycol, an additive comprising at least one C.sub.6 -C.sub.25 carboxylic acid, preferably having a pH in the range from about 7.0 to 8.5, and at a temperature ranging from about 25.degree. C. to about 37.degree. C. for a period of time ranging from about 2 hours to about 18 hours, and recovering monomeric calicheamicin derivative/carrier conjugates. Alternatively, the conjugates can be prepared by incubating the calicheamicin derivative and a proteinaceous carrier in a solution comprising a phosphate buffered solution and a cosolvent comprising t-butanol.