Abstract: Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Abstract: A technique is provided to visualize microtubules in live cells that does not require genetic manipulation or microinjection. Moreover, this method also avoids perturbation of the endogenous microtubule network that occurs with taxol treatment. This technique exploits tyrosination and detyrosination of tubulin, a posttranslational modification cycle specific to the C-terminus of ?-tubulin. Specifically, cells are grown in medium supplemented with a tyrosine derivative possessing a reactive functional group. The cellular enzyme tubulin tyrosine ligase attaches the unnatural amino acid to a single site on tubulin. Addition of fresh medium containing a suitably derivatized fluorophore then yields fluorescent tubulin, which incorporate into cellular microtubules. Importantly, the tubulin labeling approach demonstrated here does not detrimentally affect microtubule network or cell morphology. Thus we present a simple, robust labeling technique that allows microscopic analysis of microtubules in live cells.

Abstract: This invention provided a preparation method of the composite which was immobilized Paracoccus denitrificans on modified graphene oxide and its application. The composite was obtained by following the steps below: 1) Synthesis of graphene oxide; 2) Synthesis of modified graphene oxide; 3) Acclimatization and immobilization of Paracoccus denitrificans. In this invention, the raw materials were low-cost and easily obtained used in the preparation process; easy operation, convenient, and no expensive instruments during the whole process; this invention of the composite could remove DMF from wastewater completely, and with the advantages of high efficiency, good recycle performance, economical, environmentally friendly, better feasibility.

Abstract: Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.

Abstract: Isolated Lactobacillus strains are useful in preventing or delaying the development of Type 1 Diabetes (T1D). A probiotic composition comprising the Lactobacillus strains and use of the composition in T1D prevention are provided.

Abstract: The present invention relates to a method for evaluating the integrity of the cell wall of the bacteria present in a culture in the presence of an antibiotic acting on the bacterial cell wall which, from a practical point of view, allows quickly determining if a bacterium is sensitive or resistant to an antibiotic acting on the bacterial cell wall. Likewise, the present invention also relates to a lysis solution applicable in the preceding method, specifically affecting bacteria having the cell wall damaged by the action of an antibiotic acting on the bacterial cell wall, which allows distinguishing bacteria sensitive to said antibiotic from those resistant to said antibiotic.

Abstract: 2,5-Bis(benzoxazol-2?-yl)benzene-1,4-diol derivatives (Zinhbo derivatives) are used to detect zinc ions and have particular application in vivo and in vitro. Zinhbo derivatives upon excitation give a florescence response emission that can be used to determine the presence of zinc cation in solution. Zinhbo derivatives complexed with zinc cations upon excitation can produce a florescence response emission in the visible and near infrared range. Zinhbo derivatives complexed with zinc cations exhibit a large stoke shift between the excitation and emission wavelengths.

Abstract: The present invention provides a simpler and more energy efficient process for the preparation of fatty acid methyl ester (biodiesel) from sun dried whole seed capsules of Jatropha curcas integrated with value addition of seed shells, deoiled cake and crude glycerol co-product stream. More specifically, the invention relates to a method of dispensing with the need for excess methanol recovery through distillation, cost-effective resin treatment for the refining of methyl ester and utilization of co-streams for preparation of high density energy briquettes and Polyhydroxyalkanoate biodegradable polymer in efficient and cost-effective manner.

Abstract: The present invention includes new hybrid hydrogel scaffolds comprised of a polyoxyethylene-polyoxypropylene (block) copolymer (a “poloxamer”) and a self-assembling peptide, which maintain the mechanical and bioactive properties of its individual constituents (as compared to when the individual constituents are scaffolds or hydrogels by themselves). The hydrogels of the invention can include a combination of materials from different origins or with different properties that provides a hybrid material that meets the multiple needs of a scaffold for tissue engineering.

Abstract: Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Abstract: The present invention provides method, compositions, and systems for generating basal forebrain cholinergic neurons (BFCNs) using FGF8, SHH, LXH8, GBX1, or vectors encoding these ligands, as well as using such BFCNs to treat neurological disorders such as Alzheimer's disease.

Abstract: Dewaxing buffers containing a water-soluble organic solvent, methods for use thereof, and kits incorporating dewaxing buffers. The dewaxing buffers contain a water soluble organic solvent that has a boiling point of at least 80° C. The dewaxing buffers described herein can be used to quickly and efficiently remove embedding paraffin from slide-mounted tissue sections in an aqueous buffer solution at elevated temperature without fear of paraffin redeposition of the slides. In addition, the dewaxing buffers described herein can be used to perform dewaxing and HIER in a single step, which can substantially reduce protocol time and lower the risk of sample loss or damage.

Abstract: The methods of this invention involve preventing the formation of a complex between adenine and riboflavin by reducing the amount of adenine in a solution containing blood or blood components to be pathogen reduced.

Abstract: The present disclosure relates to human facilitating cells (hFC), and methods of isolating, characterizing, and using such hFCs.

Abstract: The present invention is directed to a disposable absorbent article, and particularly a sanitary napkin, having a flexure-resistant deformation element, the deformation element having a body facing surface which has a convex upward configuration when the sanitary napkin is worn. Primarily without relying on lateral compressive forces of the wearer's labia, the deformation element of the present invention relies on the lateral compressive forces of the wearer's thighs in order to form or maintain a convex upward configuration when the sanitary napkin is worn. In a preferred embodiment, the deformation element has a flexure means, and particularly a longitudinally extending flexure hinge, for inducing the body facing surface of the deformation element to have a convex upward configuration when the sanitary napkin is worn.

Abstract: An ostomy appliance for attachment around an aperture of the human body including a plate-like member having an opening extending therethrough and being adhesively attached around such body aperture, a first flange member having an annular coupling portion and being attachable over a portion thereof to the non-adhesive side of the plate-like member adjacent the opening extending therethrough, a second flange member having an annular coupling portion associated with one side thereof and a bag-like member associated with the opposite side thereof for collecting waste products therein, the coupling portion of the second flange member being cooperatively engageable with the coupling portion of the first flange member, and a sealing member located adjacent the inner most portion of one of the coupling flange portions on the side thereof facing the opening in the plate-like member, the sealing member exerting a biasing force against the opposed coupling portion when such coupling portions are engaged with each othe

Abstract: A bandage including a superabsorbent pad covered by a porous cover pad placed on a thin, flexible hydrocolloid adhesive layer leaving a portion of the adhesive layer around the pads as an adhesive border is disclosed. One side of the adhesive layer is laminated to a polymeric backing or non-woven fabric layer and a protective cover layer is applied over the pads and border portion. The protective cover layer may be silicone coated release paper and the bandage may be subjected to a vacuum thus forming the adhesive layer to the pads resulting in the border portion being substantially coplanar with the exposed surface of the cover pad overlaying the superabsorbent pad.

Abstract: A disposable absorbent article having a topsheet, a backsheet, an absorbent core, first flaps and second flaps. Each of second flaps is connected to the first flap and has a section branching upward and a section extending outward. The branching section is partially elastic. The extending section is so constructed as to be liquid-absorbent on its top surface.

Abstract: A flexible blood pressure cuff for use in measuring the blood pressure of a patient, includes an inflatable bladder having a first side and a second side. One of the bladder sides is provided with hook and loop fasteners for adjustably and removably retaining at least a portion of one of the two bladder sides against a remaining portion of the one bladder side when the bladder is folded over itself. The effective inflatable width of the bladder is thereby adjusted to accommodate the circumference of the limb of the patient so that accurate blood pressure measurements may be obtained for each patient. According to the method, the blood pressure cuff is folded over itself to produce a cuff having an effective inflatable width which is adjusted according to the circumference of the limb of the patient whose blood pressure is to be measured.

Abstract: A highly absorbent and flexible calendered and perfembossed cellulosic pulp fluff sheet for use in disposable absorbent products such as sanitary napkins, wound dressings, bandages, incontinence pads, disposable diapers and the like. The invention also extends to a method for manufacturing the highly absorbent and flexible pulp fluff sheet and its method of use in disposable absorbent products.