Patents Examined by Rebecca M Giere
  • Patent number: 10808287
    Abstract: Diagnostic devices test markers for viral infection and markers for bacterial infection to effectively assist in the rapid differentiation of viral and bacterial infections, to differentiate between colonization and active infection, and to better diagnose microbiologically unconfirmed patients. In other embodiments, detecting a presence of MxA in combination with either the bacterial biomarker C-reactive protein or the bacterial biomarker procalcitonin increases the specificity of the bacterial biomarker with a concurrent improvement in sensitivity.
    Type: Grant
    Filed: February 2, 2016
    Date of Patent: October 20, 2020
    Assignee: Rapid Pathogen Screening, Inc.
    Inventors: Robert P. Sambursky, Robert W. VanDine, Uma Mahesh Babu
  • Patent number: 10794921
    Abstract: Photonic devices, systems, and methods for detecting an analyte in a biological solution (e.g., whole blood) are provided. Representative photonic devices are optical ring resonators having nanoscale features and micron-sized diameters. Due to the compact size of these devices, many resonators can be disposed on a single substrate and tested simultaneously as a sample is passed over the devices. Typical analytes include blood cells, antibodies, and pathogens, as well as compounds indicative of the presence of blood cells or pathogens (e.g., serology). In certain embodiments, blood type can be determined through photonic sensing using a combination of direct detection of blood cells and serology. By combining the detection signals of multiple devices, the type of blood can be determined.
    Type: Grant
    Filed: July 27, 2018
    Date of Patent: October 6, 2020
    Assignees: University of Washington, Puget Sound Blood Center
    Inventors: Daniel M. Ratner, Jill M. Johnsen, James T. Kirk, José A. López, Norman D. Brault, Shaoyi Jiang
  • Patent number: 10794910
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: October 6, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10782292
    Abstract: A rapid antibiotic susceptibility test (AST) based on the detection and quantification of the movement of single bacterial cells with a plasmonic imaging and tracking (PIT) technology. The PIT-based AST detects changes in the metabolic activity of the bacterial cells long before cell replication, and allows rapid AST for both cultivable and non-cultivable strains. PIT tracks 3D movement with sub-nanometer resolution and millisecond temporal resolution. PIT also allows simultaneous measurement of the binding kinetic constants of antibiotics and bacterial metabolic state after the introduction of antibiotics.
    Type: Grant
    Filed: February 7, 2019
    Date of Patent: September 22, 2020
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Nongjian Tao, Karan Syal
  • Patent number: 10775380
    Abstract: This invention concerns patentable devices configured to capture cancer stem cells and cell clusters. After capturing such cells and/or clusters, the cancerous cells are subjected to whole genome sequencing. The resulting genomic sequence information is then compared to that for “normal” or non-diseased tissue (obtained, for example, from either the same patient, or a population sample, etc.) in order to identify the specific genetic mutation(s) present in the CSCs. Further analysis then correlates the genetic mutations with cell growth signaling pathways typically found with tumor metastases. Armed with this information, an oncologist can then develop a specifically targeted therapy that utilizes approved drugs or drug candidates undergoing clinical testing to address the identified driver mutations and thus effect a “targeted” therapy tailored to the particular patient's disease.
    Type: Grant
    Filed: April 5, 2016
    Date of Patent: September 15, 2020
    Assignee: TUMORGEN, INC.
    Inventors: Jeffrey K. Allen, Alexander G. Allen, Austin E. Allen
  • Patent number: 10774378
    Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: September 15, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Patent number: 10775374
    Abstract: The invention presents a stationary phase for detecting at least one specific analyte in a mixture. The stationary phase includes a solid phase divided into a plurality of zones for transporting liquid by capillary force. The solid phase includes at least one first zone suitable for receiving a liquid sample, a second zone on which a receptor is immobilized, wherein the receptor is suitable for binding to a ligand, and a third zone on which quantum dots are immobilized, function as FRET donors and are linked to at least one suitable FRET acceptor via at least one oligonucleotide linker. The stationary phase exhibits improved detection sensitivity and allows simultaneous and multiparametric detection of a wide variety of analytes. The invention proposes uses of the stationary phase and also presents a method for detecting at least one specific analyte in a mixture.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: September 15, 2020
    Assignee: Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V.
    Inventors: Ralph Sperling, Ralf Himmelreich, Tobias Schunck, Raphael Thiermann
  • Patent number: 10775377
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: September 15, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10775378
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: September 15, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10775376
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: January 17, 2020
    Date of Patent: September 15, 2020
    Assignee: THE UNIVERSITY OF BRITISH COLUMBIA
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10761030
    Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
    Type: Grant
    Filed: September 9, 2014
    Date of Patent: September 1, 2020
    Assignee: Labrador Diagnostics LLC
    Inventors: Elizabeth A. Holmes, Ian Gibbons, Timothy M. Kemp, John Howard, Shaunak Roy
  • Patent number: 10753933
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: August 25, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10753927
    Abstract: A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.
    Type: Grant
    Filed: October 4, 2012
    Date of Patent: August 25, 2020
    Assignee: ALERE TECHNOLOGIES GMBH
    Inventors: Thomas Kaiser, Klaus-Peter Mobius, Torsten Schulz, Thomas Uhlig, Alexander Von Schenk Zu Schweinsberg, Eugen Ermantraut, Jens Tuchscheerer
  • Patent number: 10753932
    Abstract: An immunoassay test apparatus, including: a label signal sensor that detects label signals emitted by at least one label conjugated to an antibody or antigen of an immunoassay test device such as a lateral flow test strip or microfluidic cartridge, and outputs sensor data representing the detected label signals; a label imaging component that processes the sensor data to generate label data representing spatial locations of the detected label signals; a test result image generator that processes the label data to generate test result image data representing a visual image of the spatial arrangement of the detected label signals; and a display component that displays the test result image to a user of the immunoassay test apparatus to enable the user to evaluate the result of the immunoassay test.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: August 25, 2020
    Assignee: Axxin Pty Ltd
    Inventor: William R. Hopper
  • Patent number: 10746737
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: March 12, 2020
    Date of Patent: August 18, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10725033
    Abstract: Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. Linear flow assays include nanoparticles with high affinity binding to the analyte. Binding of the nanoparticles with an analyte in the sample is detected using thermal contrast. Analytes over a broad range of concentrations are detected in the linear flow assays. Methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader are also disclosed.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: July 28, 2020
    Assignees: REGENTS OF THE UNIVERSITY OF MINNESOTA, THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: John C. Bischof, Zhenpeng Qin, Warren Chan, Taner Akkin, Li Zhan
  • Patent number: 10722534
    Abstract: A magnetic nanoaggregate-embedded bead (NAEB), a manufacturing method thereof and a bioparticle detection method using the same are disclosed. The magnetic NAEB has a protective nanoshell, noble metal nanoparticles, Raman reporter molecules and at least one magnetic nanoparticle. The protective nanoshell covers the noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle. The noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle form a magnetic nanoaggregate. Preferably, a chemical modification is performed on the magnetic NAEB, such that at least one targeting molecule is formed on an outer wall of the protective nanoshell, wherein a type of the targeting molecule is corresponding to a type of the bioparticle to be detected.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: July 28, 2020
    Assignee: National Chung Cheng University
    Inventors: Lai-Kwan Chau, Shih-Ying Yen
  • Patent number: 10718762
    Abstract: Herein is reported a method for the determination of the simultaneous binding of a bispecific antibody to a first and a second antigen comprising the steps of a) incubating a cell expressing cell-membrane bound FRET-donor-tagged first antigen and FRET-acceptor-tagged second antigen with the bispecific antibody, and b) determining the simultaneous binding of the bispecific antibody by determining the energy transfer from the FRET-donor to the FRET-acceptor.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: July 21, 2020
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Stefan Seeber, Jens Fischer, Georg Fertig
  • Patent number: 10718768
    Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
    Type: Grant
    Filed: March 12, 2020
    Date of Patent: July 21, 2020
    Assignee: The University of British Columbia
    Inventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
  • Patent number: 10712282
    Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
    Type: Grant
    Filed: September 9, 2014
    Date of Patent: July 14, 2020
    Assignee: Labrador Diagnostics LLC
    Inventors: Elizabeth A. Holmes, Ian Gibbons, Timothy M. Kemp, John Howard, Shaunak Roy