Abstract: The disclosure provides methods using mixed substrate cofeeding for bioproduct synthesis, which enables faster, more efficient, and higher yield carbon conversion in various organisms.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
May 16, 2023
Assignee:
Massachusetts Institute of Technology
Inventors:
Junyoung Park, Nian Liu, Gregory Stephanopoulos
Abstract: A method is shown for preparing a specimen for extensively analyzing a resident skin bacterium and/or a substance present in the stratum corneum, such as a protein or a lipid, or observing the shape of the stratum corneum. A method for preparing a specimen for analyzing a resident skin bacterium and/or a substance present m the stratum corneum or observing the shape of the stratum, includes applying a solution containing a polyvinyl alcohol polymer to the skin so as to form a thin-film and peeling and collecting the formed thin-film.
Abstract: A method for activating a cell-signaling pathway of interest in a cell, including applying a time-modulated localized alternating current electrical field to the cell, wherein the amplitude and frequency of the localized alternating current electrical field is selected to activate the cell signaling pathway of interest, thereby activating the cell signaling pathway.
Type:
Grant
Filed:
November 5, 2019
Date of Patent:
April 25, 2023
Assignees:
Arizona Board of Regents on behalf of Arizona State University, The Regents of the University of California
Inventors:
Quan Qing, John Albeck, Liang Guo, Min Zhao, Houpu Li
Abstract: The present invention relates to selected strains of lactic bacteria for use in the treatment of infections caused by pathogenic bacteria belonging to the species Propionibacterium acnes, in particular for use in the preventive and/or curative treatment of dermatitis in general, seborrheic dermatitis, rosacea, eczema and acne. In addition, the present invention relates to a composition which comprises a mixture comprising or, alternatively, consisting of selected strains of lactic bacteria for use in the treatment of infections caused by pathogenic bacteria belonging to the species Propionibacterium acnes, in particular for use in the preventive and/or curative treatment of dermatitis in general, seborrheic dermatitis, rosacea, eczema and acne.
Abstract: The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.
Type:
Grant
Filed:
May 29, 2020
Date of Patent:
April 11, 2023
Assignee:
OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF
AGRICULTURAL SCIENCES
Abstract: The present invention relates to a method for extracting nucleic acids from a biological sample, and the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from biological samples in the related art, various impurities present in the biological samples are not properly removed, such that the purification rate is low, but the present invention provides a method for extracting nucleic acids from a biological sample of which the bacteria, virus and nucleic acid recovery rates are enhanced, by adding a surfactant and a sodium sulfate (Na2SO4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected more sensitively and accurately.
Abstract: The present disclosure provides isolated Bacillus strains, as well as compositions comprising one or more of the isolated strains and methods of using the isolated strains and compositions to enhance crop growth/yield.
Type:
Grant
Filed:
January 3, 2018
Date of Patent:
April 4, 2023
Assignee:
NOVOZYMES BIOAG A/S
Inventors:
Leah Blasiak, William Nathan Cude, Yaowei Kang, Timothy Liburn, Kate Brandon Sutton, Jonathan Pham
Abstract: The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and/or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.
Type:
Grant
Filed:
April 16, 2020
Date of Patent:
February 28, 2023
Assignee:
General Electric Company
Inventors:
Vasile Bogdan Neculaes, Andrew Soliz Torres, Steve Lambert Klopman
Abstract: This invention relates generally to water-insoluble but water-swellable and deformable crosslinked PEGylated microgel particles of proteins and protein-based macromolecules that are pseudoplastic (shear thinning) and flow in aqueous media under shear and which can be injected or made to flow, wherein said microgel particles can reform as a cluster of microgel particles when shearing forces are removed. The microgel particles function as a matrix to support cell growth, viability, and proliferation.
Type:
Grant
Filed:
June 15, 2021
Date of Patent:
February 28, 2023
Assignee:
ROCHAL TECHNOLOGIES LLC
Inventors:
Joseph Charles Salamone, Ann Beal Salamone, Katelyn Elizabeth Reilly, Laura Jean Suggs, Eunna Chung, Kelly Xiaoyu-Chen Leung
Abstract: Embodiments of the invention describe to methods of diagnosing, classifying, and/or identifying a patient's risk of developing graft versus host disease, including severe or lethal graft versus host disease, after receiving hematopoietic cellular transplantation, a transfusion or a transplantation, but before the onset of clinical symptoms.
Type:
Grant
Filed:
October 20, 2017
Date of Patent:
February 14, 2023
Assignee:
Icahn School of Medicine at Mount Sinai
Inventors:
James Ferrara, John E. Levine, Umut Ă–zbek
Abstract: A test specimen for detecting or determining a spatial distribution of microbes, the test specimen having measuring surfaces and a plurality of microbe collecting elements on each of the measuring surfaces. The invention also relates to a test chamber with such a test specimen, to an associated use and to an associated method.
Abstract: Antimicrobial compositions comprising one or more compound components generally recognized as safe for human consumption, and related methods of use, such compositions and methods as can be employed in a wide range of agricultural, industrial, building, pharmaceutical and/or personal care products and applications.
Type:
Grant
Filed:
May 20, 2019
Date of Patent:
February 7, 2023
Assignee:
Jeneil Biosurfactant Company, LLC
Inventors:
Niranjan R. Gandhi, Victoria Palmer Skebba, Gary A. Strobel
Abstract: Antimicrobial compositions comprising one or more compound components generally recognized as safe for human consumption, and related methods of use, such compositions and methods as can be employed in a wide range of agricultural, industrial, building, pharmaceutical and/or personal care products and applications.
Type:
Grant
Filed:
May 6, 2019
Date of Patent:
January 31, 2023
Assignee:
Jeneil Biosurfactant Company, LLC
Inventors:
Niranjan R. Gandhi, Victoria Palmer Skebba, Gary A. Strobel
Abstract: The present invention discloses a mutant of cyclodextrin glycosyltransferase and belongs to the fields of gene engineering and enzyme engineering. According to the present invention, a mutant having higher disproportionation activity of cyclodextrin glycosyltransferase is obtained by mutating the cyclodextrin glycosyltransferase. The disproportionation activity of enzymes of mutants V6D, S90G, T168A, T171A, T383A, G608A, and V6D/S90G/T168A/T171A/T383A/G608A, is respectively 1.89 times, 1.21 times, 1.21 times, 1.22 times, 1.32 times, 2.03 times, and 3.16 times that of the wild type enzyme in shake flask fermentations.
Abstract: New use of ADAMTS13 in the clinical filed is provided. The use of ADAMTS13 as a biomarker for monitoring the onset of liver damage, hepatic ischemia/reperfusion injury or the liver function after liver transplantation: a method of testing liver damage, a method of testing hepatic ischemia/reperfusion injury, or a method of testing the liver function after liver transplantation, each of the methods comprising measuring or monitoring the ADAMTS13 activity in a sample from a mammal; an agent for treating diseases selected from the group consisting of liver damage, hepatic ischemia/reperfusion injury and hepatic dysfunction after liver transplantation, which comprises ADAMTS13 or a mutant of ADAMTS13 as an effective ingredient.
Type:
Grant
Filed:
January 6, 2017
Date of Patent:
January 31, 2023
Assignees:
KYOTO UNIVERSITY, KM BIOLOGICS CO., LTD.
Abstract: Systems and methods for processing biological fluids are disclosed. The systems and methods use a reusable hardware unit and a disposable fluid processing circuit that mounts onto the reusable hardware unit. The system and method, under the direction of a pre-programmed controller allow for automatically, opening one or more flow paths to effect addition of one or both parts of an additive solution to a biological fluid component.
Abstract: According to one embodiment, a microcapsule for selective catalysis of gases, the microcapsule comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In more embodiments, methods of forming such microcapsules include: emulsifying at least one biocatalyst in a polymer precursor mixture; emulsifying the polymer precursor mixture in an aqueous carrier solution; crosslinking one or more polymer precursors of the polymer precursor mixture to form a plurality of microcapsules each independently comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In further embodiments, corresponding methods of using the inventive microcapsules for catalyzing one or more target gases using include: exposing a plurality of the biocatalytic microcapsules to the one or more target gases.
Type:
Grant
Filed:
January 20, 2017
Date of Patent:
January 3, 2023
Assignee:
Lawrence Livermore National Security, LLC
Inventors:
Sarah Baker, Joshuah K. Stolaroff, Congwang Ye
Abstract: Compositions comprising microbiological strains and cultures and methods of use thereof are provided herein. Certain strains, cultures, and compositions thereof are useful for the control of head blight disease, for example, of various crop plants. Biological control compositions, and methods of use thereof to prevent, inhibit or treat the development of plant pathogens or disease and for preserving plant yield, are also provided.
Type:
Grant
Filed:
July 17, 2019
Date of Patent:
December 20, 2022
Assignee:
MONSANTO TECHNOLOGY LLC
Inventors:
Christopher J. Grandlic, Wayne A. Green, Janne S. Kerovuo, Ryan T. McCann
Abstract: Disclosed is a nanoparticle system consisting of a polymer support or substrate in the form of nanoparticles to which a hydrolase enzyme able to degrade hyaluronic acid and one or more biologically and/or pharmacologically active molecules are covalently bonded, its preparation process and its uses in the diagnostic, prognostic and therapeutic fields.
Abstract: Methods and systems for using encapsulated microbubbles to process biological samples are disclosed. According to one aspect, a method for using encapsulated microbubbles to process a biological sample includes creating a mixture comprising encapsulated microbubbles mixed with a biological sample and adding activation energy to the mixture to cause at least some of the microbubbles to oscillate or burst and thereby process the sample, including effecting cell lysis, shearing DNA, and/or performing tissue dispersion.
Type:
Grant
Filed:
May 25, 2018
Date of Patent:
November 1, 2022
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Inventors:
William Perry Janzen, Samantha Gail Pattenden, Chatura Nadisha Jayakody, Jason Eric Streeter, Paul Alexander Dayton, Cameron Champion Wood, Siddharth Kaup Shenoy