Abstract: The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications.

Abstract: The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Abstract: The present invention relates to dry cell culture media comprising amino acid components of certain particle size. Some dry powder cell culture media show poor dissolving properties and result in turbid solutions when they are dissolved in aqueous solutions. Using amino acid components of certain particle sizes significantly reduces that problem.

Abstract: The present invention provides a novel lactic acid bacterium having antioxidant and anti-aging functions and a dementia alleviation effect, and more specifically, provides Lactobacillus pentosus var. plantarum C29 KCCM11291P and Lactobacillus curvatus C3 KCCM430009. In addition, the present invention provides an antioxidant and anti-aging pharmaceutical composition and a dietary supplement containing the Lactobacillus pentosus var. plantarum C29 KCCM11291P or Lactobacillus curvatus C3 KCCM430009 of the present invention as an active ingredient, and a composition for preventing or treating dementia and a dietary supplement for preventing or alleviating dementia, containing Lactobacillus pentosus var. plantarum C29 KCCM11291P or Lactobacillus curvatus C3 KCCM430009 as an active ingredient. Additionally, the present invention provides a composition for preventing or treating dementia, containg a fermentation composition, as an active ingredient, fermented by Lactobacullus pentosus var.

Abstract: The invention relates to the cosmetic use of an effective amount of at least one monounsaturated fatty acid, of a salt thereof and/or of an ester thereof, as a nailcare active agent.

Abstract: The present specification discloses papermaking additive compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to increase separation of cellulose fibers from a pulp, to remove one or more impurities and/or one or more contaminates from a pulp and/or a paper material and to remove an ink from a pulp and/or a paper material.

Abstract: The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.

Abstract: Disclosed is a composition for embedded three-dimensional microbial culture, the composition including nanofibrillar cellulose and at least one nutrient source. Also disclosed is a method for the manufacture of a composition for embedded three-dimensional microbial culture, the method including the steps of providing nanofibrillar cellulose, mixing the nanofibrillar cellulose with water and at least one nutrient source and optional additives to obtain a mixture, and optionally drying the mixture.

Abstract: A composition for fermentation or microbial culture of gram-positive bacteria, includes fibril cellulose and at least one nutrient source including at least one carbon source, at least one nitrogen source, at least one phosphorus source, at least one mineral source and at least one trace element source.

Abstract: The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.

Abstract: A stamp and a method for transferring particles to cells of a cell culture. The stamp includes a body having upper and lower surfaces. The lower surface includes a recessed portion. A gel extends along the recessed portion of the lower surface of the body and including the particles patterned therein. The body is configured to move between a first position wherein the gel is isolated from the cells of the cell culture and a second position wherein the particles patterned in the gel communicate with the cells of the cell culture through diffusion.

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Abstract: The invention is based on the use of photoluminescent probes for intracellular sensing of oxygen, especially assaying intracellular oxygen concentration. The photoluminescent probe comprises a suspension of polymeric particles having an average diameter in the 20 nm to 100 nm range, formed from an amphiphilic cationic co-polymer which is oriented in the formed particle to provide a hydrophobic core and a hydrophilic shell. The probe includes a hydrophobic oxygen-sensitive photoluminescent dye such as Pt-tetrakis(pentafluorophenyl)porphine, PtPFPP embedded in the hydrophobic core of the particle, and the co-polymer includes quaternary ammonium groups which provide hydrophilic and cationic character to the particle shell. The photoluminescent probe, which in use is provided in the form of an aqueous suspension of probe, is incubated with live mammalian cells in a suitable growth medium for a period of time to allow the probe particles passively load into the cells.

Abstract: The present invention relates to a medium composition containing an Ecklonia cava extract for dedifferentiation into an induced pluripotent stem cell. Also, the present invention relates to a method for differentiating an induced pluripotent stem cell produced by using the medium composition into adipocytes.

Abstract: According to the present invention, the enzyme activity of an immobilized enzyme is improved regardless of enzyme type. A bioreactor, comprising an immobilized enzyme and a heterocyclic compound containing nitrogen and carbon atoms and having 5- or 6-membered ring, which form a reaction field, wherein the immobilized enzyme is allowed to cause an enzymatic reaction in the reaction field is, provided.

Abstract: The present invention relates to an in-situ method for preparing rebaudioside A from sucrose and stevioside by sucrose synthase and glycosyltransferase.

Abstract: An object of the present invention is to develop a means capable of maintaining the enzymatic activity of a microorganism even in a dry state. Provided is a method for producing a dry microbial cell powder maintaining an enzyme titer, characterized by comprising adding a carbohydrate to a liquid of microbial cells having an enzymatic activity and then drying the liquid. Also provided is a dry microbial cell powder obtained by the method.

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: The present invention relates to an aqueous solution for organ preservation, in particular for the myocardium, the solution comprising: 100 mM to 130 mM of Na+; 12 mM to 15 mM of K+; 0.25 mM to 1.3 mM of Ca++; 0.5 ?M to 1.5 ?M of cyclosporin A; and 0.5 ?M to 1.5 ?M of taxol, and it also relates to the joint use of cyclosporin A and taxol in a solution for in vitro preservation of an organ, in particular the myocardium.

Abstract: A ratio of rare cells to non-rare cells in a blood sample suspected of containing rare cells and non-rare cells is enhanced. A treated blood sample is prepared by providing in combination the blood sample, a platelet deactivation agent, a fibrin-formation-arresting agent and fibrin in an amount sufficient to cause a predetermined level of agglutination of the rare cells. The treated blood sample is then contacted with a porous matrix such that agglutinated rare cells are preferentially retained on the porous matrix. The rare cells may then be identified.