Abstract: The present invention relates to a process for forming or obtaining vivianite in or from a phosphorus-containing waterbody, sediment and/or sludge, to an apparatus for obtaining vivianite from a phosphorus-containing waterbody, sediment and/or sludge, and to the use of a composition comprising at least one alkaline earth metal peroxide and a magnetic separating apparatus for obtaining vivianite from a phosphorus-containing waterbody, sediment and/or sludge.

Abstract: Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate O-acetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.

Abstract: Provided herein are compositions, systems, and methods for delivering an effector protein into a cell. The present disclosure, in some aspects, provide novel proteins delivering an effector protein into a cell. The novel proteins are supernegatively charged proteins derived from highly anionic proteins identified from the proteome (e.g., human proteome). The novel protein tags can be associated (e.g., covalently or nocovalently) with the protein to be delivered to facilitate delivery of the effector protein into a cell.

Abstract: The present invention is directed to the development of novel photocrosslinking activity based probes (ABPs) and their uses. Specifically, ubiquitin-charged E2 conjugating enzymes are engineered and shown to be effective ABPs of RING ubiquitin E1 and E3 ligases as well as deubiquitination enzymes.

Abstract: The invention concerns a process for the preparation of fish proteases from fish viscera, preferably from cod (Gadus genus) viscera. The fish proteases produced according to the invention have high specific enzymatic activity and are useful for food uses, for biomedical applications, in histology and tissue culture.

Abstract: This disclosure relates to microbial technology, and more particularly to a Schizochytrium strain and a use thereof, a microbial oil containing DHA at an Sn-2 position, and a preparation and uses thereof. Sn-2 fatty acids of a triglyceride in the microbial oil contain 23% or more by weight of DHA. The microbial oil is prepared by fermentation using a Schizochytrium strain, where the Schizochytrium strain is Schizochytrium sp. with an accession number of GDMCC No. 60733.

Abstract: The present invention relates to a method for obtaining a fungal mat comprising the steps of: a) inoculating and growing a filamentous fungal species onto a solid nutritive medium comprising a lignocellulosic material, thereby obtaining a solid nutritive medium colonized by said fungal species; b) mixing said colonized nutritive medium with water or with an aqueous solution and blending at high speed to obtain a homogeneous living fungal slurry; c) pouring the living fungal slurry into a flat container; d) incubating the living fungal slurry until a continuous fungal mat of the desired thickness and density is formed on the top surface of the living fungal slurry; e) harvesting the fungal mat thus obtained; and, optionally f) washing the harvested fungal mat.

Abstract: Provided are compositions and methods based in part on the discovery that Enterococcus faecium secreted antigen A (SagA)-expressing bacteria are protective against enteric infections and enhances immune checkpoint inhibitor efficacy against cancer. Bacteria that express SagA or heterologous SagA, or orthologs thereof, are provided, and are included as nutraceutical, pharmaceutical, and probiotic formulations, as well as components of food products, including dairy products.

Abstract: [Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater. [Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating/pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-methoxybenzyl alcohol from the heated/pressurized product. The plankton is a diatom.

Abstract: The present invention provides engineered acetate kinase (AcK) enzymes, polypeptides having AcK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AcK enzymes are also provided. The present invention further provides compositions comprising the AcK enzymes and methods of using the engineered AcK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Abstract: Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, 1312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H: quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\?-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

Abstract: A recombinant methanol utilization pathway deficient methylotrophic yeast (Mut?) host cell which is engineered: a) by one or more genetic modifications to reduce expression of a first and a second endogenous gene compared to the host cell prior to said one or more genetic modifications, wherein i. the first endogenous gene encodes alcohol oxidase 1 (AOX1) comprising the amino acid sequence identified as SEQ ID NO:1 or a homologue thereof, and ii. the second endogenous gene encodes alcohol oxidase 2 (AOX2) comprising the amino acid sequence identified as SEQ ID NO:3 or a homologue thereof, and b) by one or more genetic modifications to increase expression of an alcohol dehydrogenase (ADH2) gene compared to the host cell prior to said one or more genetic modifications, wherein the ADH2 gene encodes an alcohol dehydrogenase (ADH2).

Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.

Abstract: The present disclosure provides methanotrophic bacteria that are modified to produce less glycogen, and methods of using the modified methanotrophic bacteria to produce a desired product, such as protein(s) or metabolite(s).

Abstract: A method of treatment is disclosed, comprising administering a composition of Cyclodextrin and reduced, nanonized L-Glutathione to a patient in need of treatment, wherein the L-Glutathione molecule is non-acetylated, non-Esterified, and non-fatty acid attached.

Abstract: Provided herein are expanded allogeneic adipose tissue-derived stromal stem cells for use in treating complex perianal fistulas in Crohn's Disease.

Abstract: Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-type Aspergillus niger glucose oxidase or a mutant Aspergillus niger glucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.

Abstract: The present disclosure relates to the method for the production of iodinated proteins to be used as biologically active substances and can be used for preventing and treating of the iodine deficiency conditions in humans and animals, in particular, when producing food products, biologically active food supplements, medicinal agents and veterinary preparations and foodstuffs. To this end, a method for the production of iodinated proteins and/or their hydrolyzates is proposed, comprising fermentation of the source protein raw material with an inorganic iodine aqueous solution by introducing therein a buffer mixture of reagents with a reaction mixture of enzymes immobilized on semipermeable membranes or on inert supports. The invention provides for the production of proteins with a determinated iodinated tyrosine amino acid residues content.

Abstract: Devices and methods for delivering oxygen and other therapeutic gases to a target, such as a tissue, a tissue-engineered construct, and a wound, in a controlled and sustained manner are disclosed.

Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.