Patents Examined by Satyendra K. Singh
  • Patent number: 11919991
    Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.
    Type: Grant
    Filed: September 12, 2022
    Date of Patent: March 5, 2024
    Assignee: Carnegie Mellon University
    Inventors: Krzysztof Matyjaszewski, Alan Enciso Barros, Liye Fu, Alan J. Russell
  • Patent number: 11913055
    Abstract: Disclosed herein relates to biopharmaceuticals, and more particularly to a continuous flow method for preparing (R)-3-hydroxy-5-hexenoate. Carbonyl reductase and isopropanol dehydrogenase are co-immobilized onto an inert solid medium simultaneously to prepare a carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst, which is then filled into a microchannel reactor of the micro reaction system. A solution containing substrate 3-carbonyl-5-hexenoate is subsequently pumped into the microchannel reactor to perform an asymmetric carbonyl reduction reaction to obtain (R)-3-hydroxy-5-hexenoate.
    Type: Grant
    Filed: October 19, 2021
    Date of Patent: February 27, 2024
    Assignee: Fudan University
    Inventors: Fener Chen, Dang Cheng, Zedu Huang, Chen Hu, Meifen Jiang, Minjie Liu, Huashan Huang
  • Patent number: 11913049
    Abstract: An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: February 27, 2024
    Inventors: Ramon Gonzalez, James Clomburg, Alexander Chou
  • Patent number: 11898159
    Abstract: Methods of making a spheroid are provided whereby a suspension is first produced including one or more biologically-relevant materials dispersed within a biocompatible medium. A droplet of the suspension is then bioprinted into a salt solution by bringing the droplet into contact with a surface of the salt solution in a controlled manner to reproducibly yield a spheroid containing a desired size and a desired amount of biologically-relevant materials.
    Type: Grant
    Filed: December 17, 2020
    Date of Patent: February 13, 2024
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Stuart K. Williams, Jeremy S. Touroo, James B. Hoying
  • Patent number: 11896639
    Abstract: The invention relates to methods of evaluating the quality of a batch of an herbal composition, the method comprising subjecting a test batch of the herbal composition to one or more biological analysis methods and comparing the results derived from the test batch to the results of a known batch of herbal composition which has a known in vivo effect.
    Type: Grant
    Filed: November 22, 2022
    Date of Patent: February 13, 2024
    Inventor: Yung-Chi Cheng
  • Patent number: 11879148
    Abstract: A further high-sensitive method for detection, determination, and activity measurement of peroxidase with no special enhancer argent. The substance, for example, high-concentration ammonium sulfate, is dissolved in the reaction solution to give rise to the micro-hydrophobic property, for detection, determination, and activity measurement of peroxidase using luminol and hydrogen peroxide as substrates.
    Type: Grant
    Filed: April 29, 2022
    Date of Patent: January 23, 2024
    Inventor: Hajime Karatani
  • Patent number: 11873477
    Abstract: Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate Oacetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.
    Type: Grant
    Filed: March 14, 2019
    Date of Patent: January 16, 2024
    Inventors: Hongwei D. Yu, Meagan E. Valentine, Richard M. Niles, Thomas Ryan Withers, Brandon D. Kirby
  • Patent number: 11864554
    Abstract: The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.
    Type: Grant
    Filed: October 17, 2022
    Date of Patent: January 9, 2024
    Assignees: The Curators of the University of Missouri, The Trustees of Columbia University in New York
    Inventors: James L. Cook, Clark T. Hung, Eric Lima, Aaron Stoker
  • Patent number: 11866753
    Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
    Type: Grant
    Filed: May 25, 2021
    Date of Patent: January 9, 2024
    Assignee: Inbicon A/S
    Inventors: Jan Larsen, Niels Nielsen Poulsen, Martin Dan Jeppesen, Kit Kellebjerg Mogensen
  • Patent number: 11859211
    Abstract: Disclosed herein are methods for generating satellite cells and compositions including satellite cells.
    Type: Grant
    Filed: November 23, 2022
    Date of Patent: January 2, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: Lee L. Rubin, Feodor D. Price
  • Patent number: 11859165
    Abstract: A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
    Type: Grant
    Filed: January 17, 2023
    Date of Patent: January 2, 2024
    Assignee: EMULATE, INC.
    Inventors: Daniel Levner, Christopher David Hinojosa, Norman Wen, Antonio Varone, Justin Nguyen, Lina Williamson, S. Jordan Kerns, Catherine Karalis, Geraldine Hamilton, Carol Lucchesi
  • Patent number: 11851645
    Abstract: Commercially available Quinoa grain powder is subjected to proteolytic digestion using papain enzyme. Derived hydrolysate is ultrafiltered using two sequential ultrafiltration steps of 50 KDa and 5 KDa membranes to remove high and low molecular impurities respectively. Concentrated filtrate is 0.2 ? filter-sterilized and used as a component growth medium for fastidious microorganisms Neisseria meningitidis and Streptococcus pneumoniae. The product is tested for its ability to be used as meat free nitrogen and carbon source for the fastidious bacterial growth and found to be working well.
    Type: Grant
    Filed: January 24, 2022
    Date of Patent: December 26, 2023
    Inventor: Seshu Kumar Gudlavalleti
  • Patent number: 11844895
    Abstract: The present invention provides methods for removing a significant amount of bacteria (e.g., gram-negative bacteria and gram-positive bacteria, including bacteria with no or low affinity for heparan sulfate) from whole blood, serum or plasma using an adsorption media. The method can be used in extracorporeal treatments involving high volumetric flow rates and high linear flow rates.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: December 19, 2023
    Assignee: ExThera Medical Corporation
    Inventors: Keith McCrea, Robert Ward
  • Patent number: 11840709
    Abstract: The present disclosure relates to methods and compositions for isolating mitochondria from cells and tissues. The methods provided herein are suitable for use in plants, animals, and fungi.
    Type: Grant
    Filed: April 9, 2021
    Date of Patent: December 12, 2023
    Inventor: Takako Nagata
  • Patent number: 11820968
    Abstract: Applications in transfusion medicine requiring platelets, and hematopoietic stem-cell transplantations require either platelets or enhancement of in vivo platelet biogenesis. Gene therapy applications of hematopoietic stem and progenitor cells (HSPCs) require effective and specific modification of HSPCs by DNA, RNA or other biological molecules. Here we disclose methods for the generation, and modification of megakaryocytic microparticles (MkMPs), proplatelets, preplatelets, platelet-like particles and megakaryocyte extracellular vesicles, that can be used in the aforementioned transfusion and transplantation medicine applications and in gene therapy applications involving hematopoietic stem cells.
    Type: Grant
    Filed: December 11, 2019
    Date of Patent: November 21, 2023
    Inventors: Eleftherios Papoutsakis, Chen-Yuan Kao, Jinlin Jiang
  • Patent number: 11820973
    Abstract: Provided herein are methods and systems for interfering with viability of bacteria and related compounds and compositions.
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: November 21, 2023
    Inventors: Dianne K. Newman, Ryan C. Hunter
  • Patent number: 11812752
    Abstract: Pandemic COVID 19 causes global crisis in human health and economy. The pathogen of COVID 19, like the Influenzas causing common flus, belongs to a common type of virus called Ribonucleic Acid (RNA) virus. RNA viruses are composed of three structural components: genomic RNA, envelop and nuclear proteins, and bilayer lipid membrane. Targeting COVID 19 and all RNA viruses, the present invention describes an effective disinfectant system, which applies the combination of RNases, proteases, and detergents, at a broad range of concentration, pH, and temperature in the utilization, to eliminate the RNA viruses from any contaminated surfaces, airways, and filters. The disinfectant system provides an effective, convenient, environment friendly, and safe solution, for commercial and customer use, to disinfect RNA virus pathogens in concern.
    Type: Grant
    Filed: April 26, 2020
    Date of Patent: November 14, 2023
    Inventor: Liming Wang
  • Patent number: 11802836
    Abstract: A process for detecting the sensitivity of one or more polymers and/or of one or more, mixtures of polymers to a compound, including the steps of exposing at least one lens-shaped micro-deposit, including the polymer(s) and/or the mixture(s) of polymers, to the compound, and detecting, by illuminating the surface of this micro-deposit, a change in the spatial distribution of the intensity of the light reflected or transmitted by this micro-deposit, linked to a variation in the dimensions and/or refractive index of this micro-deposit, under the effect of an interaction between the polymer(s) and/or the mixture(s) of polymers and the compound.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: October 31, 2023
    Assignees: Universite De Lille, Centre National De La Recherche Scientifique
    Inventors: Alexis Vlandas, Julien Wengler, Sebastien Lamant, Vincent Senez
  • Patent number: 11793124
    Abstract: A system for growing fungi, the system comprising a nutritive vehicle, a porous material, an administrable space, fungal tissue comprising fungal hyphae having a growth pattern, the fungal tissue connecting said nutritive vehicle to said porous material to said administrable space, wherein the fungal tissue within said space defines at least one successive fungal material layer; and a chemically or physically altered separated portion of fungal material, the separated portion separated from said fungal tissue.
    Type: Grant
    Filed: April 25, 2022
    Date of Patent: October 24, 2023
    Assignee: Myco Works, Inc.
    Inventors: Philip Ross, Nicholas Wenner, Caitlin Moorleghen
  • Patent number: 11795482
    Abstract: The present invention relates to the preparation of organic molecules by anaerobic fermentation of biomass, in which the fermentation liquor is aerated prior to the organic molecule recovery step.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: October 24, 2023
    Assignee: AFYREN
    Inventors: Jeremy Jean-Paul Pessiot, Michael Frederic Pierre Roussel, Aurelien Antoine Bost