Abstract: Pandemic COVID 19 causes global crisis in human health and economy. The pathogen of COVID 19, like the Influenzas causing common flus, belongs to a common type of virus called Ribonucleic Acid (RNA) virus. RNA viruses are composed of three structural components: genomic RNA, envelop and nuclear proteins, and bilayer lipid membrane. Targeting COVID 19 and all RNA viruses, the present invention describes an effective disinfectant system, which applies the combination of RNases, proteases, and detergents, at a broad range of concentration, pH, and temperature in the utilization, to eliminate the RNA viruses from any contaminated surfaces, airways, and filters. The disinfectant system provides an effective, convenient, environment friendly, and safe solution, for commercial and customer use, to disinfect RNA virus pathogens in concern.

Abstract: A process for detecting the sensitivity of one or more polymers and/or of one or more, mixtures of polymers to a compound, including the steps of exposing at least one lens-shaped micro-deposit, including the polymer(s) and/or the mixture(s) of polymers, to the compound, and detecting, by illuminating the surface of this micro-deposit, a change in the spatial distribution of the intensity of the light reflected or transmitted by this micro-deposit, linked to a variation in the dimensions and/or refractive index of this micro-deposit, under the effect of an interaction between the polymer(s) and/or the mixture(s) of polymers and the compound.

Abstract: A system for growing fungi, the system comprising a nutritive vehicle, a porous material, an administrable space, fungal tissue comprising fungal hyphae having a growth pattern, the fungal tissue connecting said nutritive vehicle to said porous material to said administrable space, wherein the fungal tissue within said space defines at least one successive fungal material layer; and a chemically or physically altered separated portion of fungal material, the separated portion separated from said fungal tissue.

Abstract: The present invention relates to the preparation of organic molecules by anaerobic fermentation of biomass, in which the fermentation liquor is aerated prior to the organic molecule recovery step.

Abstract: The present invention relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host by reducing the propensity to diabetes, obesity and metabolic syndrome mediated in part by production of short chain fatty acids to enhance colonic butyrate production. Therapeutic methods of the invention involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will improve significantly the ability to control weight, reduce the onset of diabetes, obesity and metabolic syndrome, and improve overall health.

Abstract: The subject invention provides improved methods for producing mannosylerythritol lipids (MEL) using yeasts not previously known to produce MEL. In particular, Meyerozyma guilliermondii (Pichia guilliermondii) is cultivated in a specially-tailored nutrient medium and under cultivation conditions such that the yeast unnaturally produces MEL and/or MEL-like molecules in greater amounts and at increased rates than when using standard MEL production with, for example, Pseudozyma aphidis. Yeast culture compositions are also provided, comprising yeast cells, growth medium, and high concentrations of MEL.

Abstract: The disclosure provides compositions comprising exosome subpopulations, and methods of their use in subjects having certain disorders including lung disorders, cardiovascular disorders, renal disorders and ischemic neural disorders.

Abstract: A method of detecting specified target microbes in different types of sample uses only one to two cultivation steps for the enrichment of the target microbes from the sample, preferably in selective culture media, combined with a mass spectrometric detection method that identifies the target microbes in mixtures with other microbes even if the target microbes account for only a small proportion of the mixture. The sample may be a food sample, a sample from bodies of water used for bathing, a soil sample, a swabbed sample, a stool sample, an impactor sample with collected aerosol particles, amongst many others. The detection method is several days faster than standard methods and less expensive.

Abstract: Methods and compositions for enhancing or promoting germination of bacterial spores, and yeasts are disclosed herein. The composition of the present invention comprises an extract obtained from banana or any member belonging to the genus Musa that may be used alone or in a growth medium to promote and enhance germination of bacterial spores, growth of bacterial, yeast, and fungal cell cultures.

Abstract: Provided herein is a method and kit for diagnosing and treating Graft versus host disease (GVHD), the method including obtaining a body fluid or tissue sample from said mammal, throughout the year post-transplant; detecting the expression level, phosphorylation level, or a combination thereof, of biomarkers in said body fluid or tissue sample; diagnosing said mammal with Graft versus host disease (GVHD) when the expression level, phosphorylation level, or a combination thereof of said biomarkers in said body fluid or tissue sample are detected with a deviation of at least about 25% from standard measurements taken from healthy subjects and/or from measurements taken from said mammal prior to said transplant; and, administering an effective amount of at least one immunosuppressant or steroid or a combination thereof to the diagnosed mammal.

Abstract: Provided herein are methods for delaying or inhibiting T cell maturation or differentiation in vitro for a T cell therapy, comprising contacting one or more T cells from a subject in need of a T cell therapy with an AKT inhibitor and at least one of exogenous Interleukin-7 (IL-7) and exogenous Interleukin-15 (IL-15), wherein the resulting T cells exhibit delayed maturation or differentiation. In some embodiments, the method further comprises administering the one or more T cells to a subject in need of a T cell therapy.

Abstract: Methods and compositions for the treatment of Brucella induced diseases and disorders are disclosed herein. In preferred embodiments, the invention relates to a vaccine compositions and methods of vaccinating comprising: a Brucella canis comprising one or more attenuating gene knockouts; and a pharmaceutically acceptable vaccine carrier.

Abstract: Described herein is a discovery Platform Technology for analyzing a drug-induced toxicity condition, such as cardiotoxicity via model building.

Abstract: The present invention relates to a method for screening an inhibitor of ATP11C or CDC50A, comprising determining (a) exposure of phosphatidylserine on cell surface, (b) engulfment of cells by macrophages, or (c) cleavage of ATP11C by caspase. The present invention also relates to a method for inducing engulfment of cells by macrophages, comprising inhibiting ATP11C or CDC50A.

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

Abstract: Yeast strains are disclosed that are capable of surviving high ethanol concentrations and propagating in high ethanol concentrations. Genes that are involved in ethanol tolerance are disclosed as well.

Abstract: A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.

Abstract: A method, a medium and a kit for the enrichment and detection of Listeria species, especially Listeria monocytogenes. The medium is an enrichment medium of C12 to C16 fatty acids and/or derivatives thereof.

Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

Abstract: The present invention relates to the field of diagnostics and more particularly to the detection of beta-hemolytic pathogens. More specifically, the present invention relates to the rapid and accurate detection of beta-hemolytic pathogens using sterically-stabilized liposomes.