Patents Examined by Shafiqul Haq
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Patent number: 11906524Abstract: The present subject matter provides urea biosensors as well as compositions, devices, and methods comprising such biosensors.Type: GrantFiled: November 19, 2016Date of Patent: February 20, 2024Assignee: Duke UniversityInventors: Homme W. Hellinga, Malin J. Allert
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Patent number: 11899011Abstract: An assay substrate including a first component comprising a sensor molecule labeled with a quantum dot, the quantum dot immobilized to an assay substrate surface with a first linker being a bi-polar linker comprising a first binding group for specific binding of the quantum dot and a second binding group for specific binding of the assay substrate surface, the sensor molecule having a specific binding site for an organic analyte, the sensor molecule labeled with the quantum dot in a position that has no effect on the organic analyte binding the specific binding site; and a second component comprising a chemical analogue of the organic analyte, the chemical analogue labeled with a fluorescent dye, the chemical analogue linked to the quantum dot with a second linker having a length exceeding Foster radius, and the chemical analogue reversibly binding the specific binding site of the sensor molecule of the first component.Type: GrantFiled: April 27, 2023Date of Patent: February 13, 2024Assignee: QanikDX OUInventors: Sergei Babitshenko, Jaak Järv, Aleksei Kuznetsov, Anton Mastitski
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Patent number: 11891652Abstract: The invention relates to a method of assembling a biosensor device comprising two or more biosensor units, wherein each unit comprises one or more biosensors comprising one or more carbon nanotubes (CNTs) coated with nucleic acid and one or more sensor molecules coupled to the nucleic acid, wherein each one of the one or more sensor molecules is capable of binding to a target molecule in a sample. Each biosensor unit is capable of detecting a different target molecule in a sample, and each unit comprises one or more biosensors each capable of detecting the same target molecule. The invention further relates to biosensor devices and methods for detecting target molecules in a sample using the same.Type: GrantFiled: November 23, 2018Date of Patent: February 6, 2024Assignee: QUEEN MARY UNIVERSITY OF LONDONInventors: Matteo Palma, Xinzhao Xu, Pierrick Clement
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Patent number: 11879894Abstract: This invention discloses bi-functional photoactivable fluorescent lipid probes for proximity labelling-based identification of membrane-associated proteins, and synthesis thereof. The photoactivable fluorescent lipid probe include a lipid moiety, a fluorophore, and a photoactivable moiety; where the fluorophore and the photoactivable moiety are covalently attached to the lipid moiety.Type: GrantFiled: June 3, 2020Date of Patent: January 23, 2024Assignee: Indian Institute of Science Education and ResearchInventors: Gregor Jose, Thomas Pucadyil
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Patent number: 11874280Abstract: Compositions comprising fluorescent or colored dyes and methods of using the same in combination with divalent metal salts to analyze samples are disclosed.Type: GrantFiled: March 8, 2019Date of Patent: January 16, 2024Assignee: Sony Group CorporationInventors: Melissa Jackson, Christopher Boyce, Eriko Matsui, Michael Vanbrunt, Sharat Singh, Tracy Matray
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Patent number: 11867705Abstract: Compounds and methods for use in detecting gabapentin in a sample suspected of containing gabapentin are disclosed. Gabapentin derivatives are used to produce gabapentin conjugates. A gabapentin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-gabapentin antibody. A gabapentin-detectable label may be used in a signal producing system in gabapentin assays.Type: GrantFiled: September 8, 2020Date of Patent: January 9, 2024Assignee: ARK Diagnostics, Inc.Inventors: Johnny Jose Valdez, Byung Sook Moon, Michael Kevin Helms, Alejandro A. Orozco
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Patent number: 11860158Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.Type: GrantFiled: February 22, 2019Date of Patent: January 2, 2024Assignee: BIOMÉRIEUX DEUTSCHLAND GMBHInventors: Johannes Reich, Holger Grallert
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Patent number: 11860167Abstract: Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.Type: GrantFiled: January 28, 2022Date of Patent: January 2, 2024Assignee: Ventana Medical Systems, Inc.Inventors: Brian D. Kelly, Nathan W. Polaske
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Patent number: 11850286Abstract: The present invention relates to a compound comprising an alpha-end and an omega-end, the compound comprising on the alpha-end a reactive group Q1 capable of reacting with a functional group F1 present on a biomolecule and on the omega-end a target molecule, the compound further comprising a group according to formula (1) or a salt thereof: Said compound may also be referred to as a linker-conjugate. The invention also relates to a process for the preparation of a bioconjugate, the process comprising the step of reacting a reactive group Q1 of a linker-conjugate according to the invention with a functional group F1 of a biomolecule. The invention further relates to a bioconjugate obtainable by the process according to the invention.Type: GrantFiled: October 5, 2020Date of Patent: December 26, 2023Assignee: SYNAFFIX B.V.Inventors: Jorge Merijn Mathieu Verkade, Maria Antonia Wijdeven, Petrus Josephus Jacobus Maria Van De Sande, Sander Sebastiaan Van Berkel, Floris Louis Van Delft
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Patent number: 11835518Abstract: Disclosed herein are immunoassays for detecting an anti-thyroid peroxidase antibody in a biological sample from a subject and/or diagnosing a thyroid disease in a subject. The disclosed immunoassays employ a recombinant cynomolgus monkey thyroid peroxidase (rTPO) and assess the level of anti-thyroid peroxidase antibody-induced formation or disruption of complexes comprising a solid support and the rTPO.Type: GrantFiled: April 6, 2022Date of Patent: December 5, 2023Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Kayla Cortese, Saji Cherian, Paul D'Agostino, Gabriel Hammarquist, Aili Han, Kuldeep Jaggi, Maral Poladian, Niver Sahakian, Seema Sinha, Prakash Tewari, Haekyung Lee
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Patent number: 11821901Abstract: Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.Type: GrantFiled: August 15, 2020Date of Patent: November 21, 2023Assignee: AAT Bioquest, Inc.Inventors: Zhenjun Diwu, Zhen Luo, Jixiang Liu, Pengfei Dong
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Patent number: 11808770Abstract: Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.Type: GrantFiled: March 4, 2020Date of Patent: November 7, 2023Assignee: Ventana Medical Systems, Inc.Inventors: Christopher Bieniarz, Rui Hong
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Patent number: 11796540Abstract: The disclosure includes a method for determining an analyte in a sample in an interaction assay, including contacting a sample with an interaction modulator, wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM), aminodextran, carboxymethyldextran, Poly-(2-acrylamido-2-methyl-1-propanesulfonic acid (PAMPS), triethylamine, triethanolamine, taurine, or dodecylsulfate. The disclosure includes a method for determining an analyte in an interaction assay, including contacting the sample with an interaction modulator, wherein the interaction modulator is an enhancer of the interaction assay at low analyte concentrations and is a retarder of the interaction assay at high analyte concentrations and wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM) and/or Polyacrylic acid (PAA). The disclosure further relates to a kit having a detection agent specifically detecting an analyte and at least one interaction modulator.Type: GrantFiled: July 26, 2019Date of Patent: October 24, 2023Assignee: Roche Diagnostics Operations, Inc.Inventors: Georg Kurz, Eloisa Lopez-Calle, Ewelina Hegel, Josef Roedl, Eva Hoess, Joerg Kaufmann, Marisa Kirchenbauer
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Patent number: 11789029Abstract: A unique pipeline is employed for biomarker discovery that entailed domain antibody phage display, next generation sequencing analysis, and nanotechnology strategies to generate antibody mimetics are disclosed. Also disclosed are the temporal biomarkers of traumatic brain injury and their methods of use. In some embodiments, the temporal biomarkers are synthetic peptides comprising the HCDR3 sequences identified using the disclosed pipeline. In some aspects, the synthetic peptides have less than 30 amino acid residues and comprise a biotin scaffold that is linked to the HCDR3 sequences.Type: GrantFiled: June 19, 2020Date of Patent: October 17, 2023Assignee: Arizona Board of Regents on Behalf of Arizona State UniversityInventors: Briana Martinez, Sarah Stabenfeldt, Chris Diehnelt, Nicholas Stephanopoulos, Crystal Willingham, Amanda Witten, Kendall Lundgreen
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Patent number: 11781955Abstract: This disclosure provides IR700-molecule conjugates and methods of their use to remove (e.g., separate or isolate) a target from a sample in vivo or from a subject in vitro. It is shown herein that exposure of IR700 to near infrared (NIR) light removes a portion of IR700, changing it from a hydrophilic molecule, to one that is hydrophobic, resulting in aggregation of IR700 and anything bound to it. For example, the disclosed IR700-molecule conjugates and methods provide photo-controlled ways to control the pharmacokinetics of a drug in vivo, and can be used to remove undesired agents from environmental or food samples or to isolate target molecules in a laboratory.Type: GrantFiled: September 21, 2020Date of Patent: October 10, 2023Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Hisataka Kobayashi, Peter Choyke, Martin John Schnermann
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Patent number: 11774455Abstract: A peptide aptamer for specific recognition of arginine and its application are provided. The sequence of the peptide aptamer is shown in SEQ ID No. 1. The peptide aptamer is modified by a group that improves stability, or by a fluorescent group, an isotope and an electrochemical group that provide a detection signal, or by an affinity ligand and a mercapto. According to the computer-aided molecular docking simulation prediction, the peptide aptamer that can specifically bind to L-arginine is screened, which is verified by an isothermal titration calorimeter. The peptide aptamer has the advantages of good stability, strong binding ability, high specificity and low production cost.Type: GrantFiled: November 16, 2019Date of Patent: October 3, 2023Assignee: INSTITUTE OF SUBTROPICAL AGRICULTURE, CHINESE ACADEMY OF SCIENCESInventors: Zemeng Feng, Yumin He, Lei Deng, Zhong Cao, Zhongliang Xiao, Yulong Yin
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Patent number: 11768203Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.Type: GrantFiled: March 31, 2017Date of Patent: September 26, 2023Assignee: University of Southern CaliforniaInventor: Preet M. Chaudhary
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Patent number: 11761964Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.Type: GrantFiled: January 22, 2020Date of Patent: September 19, 2023Assignee: CYANAGEN S.r.l.Inventors: Leopoldo Della Ciana, Lorenzo Biagini, Thomas Paul Jansen, Rossana Perciaccante, Manuela Vargiolu, Marina Eleonora Vettraino
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Patent number: 11754563Abstract: A device comprising a modified porous membrane is provided. The modified porous membrane comprises a polymer coating grafted to a porous membrane. The device is used for analyte detection from a biological sample using an immunoassay. The device comprises a sample application zone at one end of the device for applying a biological sample comprising a target analyte; and a detection zone present at another end of the device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first biomolecules immobilized on a modified porous membrane having a structure of Formula (I).Type: GrantFiled: November 20, 2014Date of Patent: September 12, 2023Assignee: GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTDInventors: Bing Li, Brian Christopher Bales, David Roger Moore, Jason Michael Nichols, Cathryn Ellen Olsen, William Christopher Alberts, Frank John Mondello
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Patent number: 11747333Abstract: The present invention relates to a method for detecting a target analyte using a gold nanoparticle, comprising growing a copper crystal specifically on a gold nanoparticle by treating the gold nanoparticle with a solution comprising a copper ion, a polymer having a primary or secondary amine group, and a reducing agent, a composition for amplifying a signal used in the detection method above, and a kit for detecting a target analyte comprising the composition for amplifying a signal above.Type: GrantFiled: February 14, 2018Date of Patent: September 5, 2023Assignees: KOREA FOOD & DRUG ADMINISTRATION, SEOUL NATIONAL UNIVERSITY R&DB FOUNDATIONInventors: Jwa-Min Nam, Gwangpyo Ko, Jae-Ho Kim, Jeong-Eun Park, Mouhong Lin, In Sun Joo, Jeong Su Lee