Patents Examined by Shafiqul Haq
  • Patent number: 11860158
    Abstract: The invention relates to unmasking endotoxins in compositions so that previously present, but undetectable endotoxins are rendered detectable.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: January 2, 2024
    Inventors: Johannes Reich, Holger Grallert
  • Patent number: 11850286
    Abstract: The present invention relates to a compound comprising an alpha-end and an omega-end, the compound comprising on the alpha-end a reactive group Q1 capable of reacting with a functional group F1 present on a biomolecule and on the omega-end a target molecule, the compound further comprising a group according to formula (1) or a salt thereof: Said compound may also be referred to as a linker-conjugate. The invention also relates to a process for the preparation of a bioconjugate, the process comprising the step of reacting a reactive group Q1 of a linker-conjugate according to the invention with a functional group F1 of a biomolecule. The invention further relates to a bioconjugate obtainable by the process according to the invention.
    Type: Grant
    Filed: October 5, 2020
    Date of Patent: December 26, 2023
    Assignee: SYNAFFIX B.V.
    Inventors: Jorge Merijn Mathieu Verkade, Maria Antonia Wijdeven, Petrus Josephus Jacobus Maria Van De Sande, Sander Sebastiaan Van Berkel, Floris Louis Van Delft
  • Patent number: 11835518
    Abstract: Disclosed herein are immunoassays for detecting an anti-thyroid peroxidase antibody in a biological sample from a subject and/or diagnosing a thyroid disease in a subject. The disclosed immunoassays employ a recombinant cynomolgus monkey thyroid peroxidase (rTPO) and assess the level of anti-thyroid peroxidase antibody-induced formation or disruption of complexes comprising a solid support and the rTPO.
    Type: Grant
    Filed: April 6, 2022
    Date of Patent: December 5, 2023
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Kayla Cortese, Saji Cherian, Paul D'Agostino, Gabriel Hammarquist, Aili Han, Kuldeep Jaggi, Maral Poladian, Niver Sahakian, Seema Sinha, Prakash Tewari, Haekyung Lee
  • Patent number: 11821901
    Abstract: Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.
    Type: Grant
    Filed: August 15, 2020
    Date of Patent: November 21, 2023
    Assignee: AAT Bioquest, Inc.
    Inventors: Zhenjun Diwu, Zhen Luo, Jixiang Liu, Pengfei Dong
  • Patent number: 11808770
    Abstract: Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.
    Type: Grant
    Filed: March 4, 2020
    Date of Patent: November 7, 2023
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christopher Bieniarz, Rui Hong
  • Patent number: 11796540
    Abstract: The disclosure includes a method for determining an analyte in a sample in an interaction assay, including contacting a sample with an interaction modulator, wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM), aminodextran, carboxymethyldextran, Poly-(2-acrylamido-2-methyl-1-propanesulfonic acid (PAMPS), triethylamine, triethanolamine, taurine, or dodecylsulfate. The disclosure includes a method for determining an analyte in an interaction assay, including contacting the sample with an interaction modulator, wherein the interaction modulator is an enhancer of the interaction assay at low analyte concentrations and is a retarder of the interaction assay at high analyte concentrations and wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM) and/or Polyacrylic acid (PAA). The disclosure further relates to a kit having a detection agent specifically detecting an analyte and at least one interaction modulator.
    Type: Grant
    Filed: July 26, 2019
    Date of Patent: October 24, 2023
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Georg Kurz, Eloisa Lopez-Calle, Ewelina Hegel, Josef Roedl, Eva Hoess, Joerg Kaufmann, Marisa Kirchenbauer
  • Patent number: 11789029
    Abstract: A unique pipeline is employed for biomarker discovery that entailed domain antibody phage display, next generation sequencing analysis, and nanotechnology strategies to generate antibody mimetics are disclosed. Also disclosed are the temporal biomarkers of traumatic brain injury and their methods of use. In some embodiments, the temporal biomarkers are synthetic peptides comprising the HCDR3 sequences identified using the disclosed pipeline. In some aspects, the synthetic peptides have less than 30 amino acid residues and comprise a biotin scaffold that is linked to the HCDR3 sequences.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: October 17, 2023
    Assignee: Arizona Board of Regents on Behalf of Arizona State University
    Inventors: Briana Martinez, Sarah Stabenfeldt, Chris Diehnelt, Nicholas Stephanopoulos, Crystal Willingham, Amanda Witten, Kendall Lundgreen
  • Patent number: 11781955
    Abstract: This disclosure provides IR700-molecule conjugates and methods of their use to remove (e.g., separate or isolate) a target from a sample in vivo or from a subject in vitro. It is shown herein that exposure of IR700 to near infrared (NIR) light removes a portion of IR700, changing it from a hydrophilic molecule, to one that is hydrophobic, resulting in aggregation of IR700 and anything bound to it. For example, the disclosed IR700-molecule conjugates and methods provide photo-controlled ways to control the pharmacokinetics of a drug in vivo, and can be used to remove undesired agents from environmental or food samples or to isolate target molecules in a laboratory.
    Type: Grant
    Filed: September 21, 2020
    Date of Patent: October 10, 2023
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Hisataka Kobayashi, Peter Choyke, Martin John Schnermann
  • Patent number: 11774455
    Abstract: A peptide aptamer for specific recognition of arginine and its application are provided. The sequence of the peptide aptamer is shown in SEQ ID No. 1. The peptide aptamer is modified by a group that improves stability, or by a fluorescent group, an isotope and an electrochemical group that provide a detection signal, or by an affinity ligand and a mercapto. According to the computer-aided molecular docking simulation prediction, the peptide aptamer that can specifically bind to L-arginine is screened, which is verified by an isothermal titration calorimeter. The peptide aptamer has the advantages of good stability, strong binding ability, high specificity and low production cost.
    Type: Grant
    Filed: November 16, 2019
    Date of Patent: October 3, 2023
    Inventors: Zemeng Feng, Yumin He, Lei Deng, Zhong Cao, Zhongliang Xiao, Yulong Yin
  • Patent number: 11768203
    Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: September 26, 2023
    Assignee: University of Southern California
    Inventor: Preet M. Chaudhary
  • Patent number: 11761964
    Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.
    Type: Grant
    Filed: January 22, 2020
    Date of Patent: September 19, 2023
    Assignee: CYANAGEN S.r.l.
    Inventors: Leopoldo Della Ciana, Lorenzo Biagini, Thomas Paul Jansen, Rossana Perciaccante, Manuela Vargiolu, Marina Eleonora Vettraino
  • Patent number: 11754563
    Abstract: A device comprising a modified porous membrane is provided. The modified porous membrane comprises a polymer coating grafted to a porous membrane. The device is used for analyte detection from a biological sample using an immunoassay. The device comprises a sample application zone at one end of the device for applying a biological sample comprising a target analyte; and a detection zone present at another end of the device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first biomolecules immobilized on a modified porous membrane having a structure of Formula (I).
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: September 12, 2023
    Inventors: Bing Li, Brian Christopher Bales, David Roger Moore, Jason Michael Nichols, Cathryn Ellen Olsen, William Christopher Alberts, Frank John Mondello
  • Patent number: 11747333
    Abstract: The present invention relates to a method for detecting a target analyte using a gold nanoparticle, comprising growing a copper crystal specifically on a gold nanoparticle by treating the gold nanoparticle with a solution comprising a copper ion, a polymer having a primary or secondary amine group, and a reducing agent, a composition for amplifying a signal used in the detection method above, and a kit for detecting a target analyte comprising the composition for amplifying a signal above.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: September 5, 2023
    Inventors: Jwa-Min Nam, Gwangpyo Ko, Jae-Ho Kim, Jeong-Eun Park, Mouhong Lin, In Sun Joo, Jeong Su Lee
  • Patent number: 11740238
    Abstract: Disclosed herein are methods and assays for the detection and/or quantification of one or more proteins or substances of interest in a sample, such as a plant, human, insect, microorganism or mammalian sample.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: August 29, 2023
    Inventors: Kristi Ruth Harkins, Aaron Joseph Walck
  • Patent number: 11738098
    Abstract: Embodiments of the present disclosure provide for contrast agents, methods of making contrast agents, and methods of using contrast agents, and the like.
    Type: Grant
    Filed: October 12, 2020
    Date of Patent: August 29, 2023
    Assignee: Georgia State University Research Foundation, Inc.
    Inventors: Jenny Jie Yang, Zhiren Liu, Jie Jiang, Shenghui Xue, Jinjuan Qiao, Mani Salarian
  • Patent number: 11726095
    Abstract: This invention resides in using metal complex-activated particles to bind molecules, polymers and other particles to each other, so as to produce multifunctional conjugates having controlled ratios of two or more different molecules.
    Type: Grant
    Filed: August 13, 2014
    Date of Patent: August 15, 2023
    Inventors: Nobuyoshi Joe Maeji, Chang-Yi Huang
  • Patent number: 11726098
    Abstract: Immobilising isolated mycolic acid antigens of tuberculous mycobacterial origin or a synthetic analogue thereof on a screen-printed electrode by binding of the antigens to a self-assembled monolayer comprising a thiolated hydrophobic substance to produce immobilized mycolic acids antigens in the form of a mycolic acid antigen-containing self-assembled monolayer coating on a surface of the electrode.
    Type: Grant
    Filed: December 24, 2019
    Date of Patent: August 15, 2023
    Assignee: University of Pretoria
    Inventors: Jan Adrianus Verschoor, Carl Baumeister
  • Patent number: 11725002
    Abstract: A detection reagent for comprehensively detecting an oxidized state and a glycated state of a low-density lipoprotein. While an oxidized low-density lipoprotein and a glycated low-density lipoprotein are detected with a fluolophore-labelled antibody, a lipid radical is detected with a fluorescent nitroxide 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperadine-1-oxyl (NBD-Pen).
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: August 15, 2023
    Inventors: Ken-ichi Yamada, Tomomi Ide, Yuma Ishida, Go Ichien, Keiichi Yamamoto
  • Patent number: 11719705
    Abstract: The present application provides stable peptide-based IL-17F and IL-17A capture agents and methods of use as detection agents. The application further provides methods of manufacturing IL-17F capture agents.
    Type: Grant
    Filed: June 15, 2018
    Date of Patent: August 8, 2023
    Inventors: Heather Agnew, Bert Tsunyin Lai, Suresh Mark Pitram, Blake Farrow, James R. Heath, David Bunck, Jingxin Liang, Arundhati Nag, Samir Das
  • Patent number: 11714082
    Abstract: A method for measuring the presence of calprotectin (S100A8/A) heterodimer in a biological sample using a particle-enhanced turbidimetric immunoassay (PETIA) based on monoclonal antibodies. The method can be adapted on automated standard analyzers and provides a reliable clinical measurement of calprotectin in faecal samples and extracts. The method is comparable to commerical two-site sandwich ELISA. The disclosed method counters spontaneous agglutination caused by calcium ions and low-molecular weight calcium-binding S100 proteins as observed with conventional PETIAs. The method can be used for measuring the presence of human calprotectin in stool, urine, serum, plasma, synovial liquid and other body liquids. Metrological traceability and high commutability with conventional immunoassays (ELISA) has been shown despite of different measurement principles used.
    Type: Grant
    Filed: May 9, 2018
    Date of Patent: August 1, 2023
    Assignees: Immundiagnostik AG, DiaSys Diagnostic Systems GmbH
    Inventors: Franz-Paul Armbruster, Matthias Grimmler, Pia Schu, Tobias Becker, Felix Walzer