Abstract: This disclosure provides IR700-molecule conjugates and methods of their use to remove (e.g., separate or isolate) a target from a sample in vivo or from a subject in vitro. It is shown herein that exposure of IR700 to near infrared (NIR) light removes a portion of IR700, changing it from a hydrophilic molecule, to one that is hydrophobic, resulting in aggregation of IR700 and anything bound to it. For example, the disclosed IR700-molecule conjugates and methods provide photo-controlled ways to control the pharmacokinetics of a drug in vivo, and can be used to remove undesired agents from environmental or food samples or to isolate target molecules in a laboratory.
Type:
Grant
Filed:
September 21, 2020
Date of Patent:
October 10, 2023
Assignee:
The United States of America, as represented by the Secretary, Department of Health and Human Services
Inventors:
Hisataka Kobayashi, Peter Choyke, Martin John Schnermann
Abstract: A peptide aptamer for specific recognition of arginine and its application are provided. The sequence of the peptide aptamer is shown in SEQ ID No. 1. The peptide aptamer is modified by a group that improves stability, or by a fluorescent group, an isotope and an electrochemical group that provide a detection signal, or by an affinity ligand and a mercapto. According to the computer-aided molecular docking simulation prediction, the peptide aptamer that can specifically bind to L-arginine is screened, which is verified by an isothermal titration calorimeter. The peptide aptamer has the advantages of good stability, strong binding ability, high specificity and low production cost.
Type:
Grant
Filed:
November 16, 2019
Date of Patent:
October 3, 2023
Assignee:
INSTITUTE OF SUBTROPICAL AGRICULTURE, CHINESE ACADEMY OF SCIENCES
Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.
Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.
Type:
Grant
Filed:
January 22, 2020
Date of Patent:
September 19, 2023
Assignee:
CYANAGEN S.r.l.
Inventors:
Leopoldo Della Ciana, Lorenzo Biagini, Thomas Paul Jansen, Rossana Perciaccante, Manuela Vargiolu, Marina Eleonora Vettraino
Abstract: A device comprising a modified porous membrane is provided. The modified porous membrane comprises a polymer coating grafted to a porous membrane. The device is used for analyte detection from a biological sample using an immunoassay. The device comprises a sample application zone at one end of the device for applying a biological sample comprising a target analyte; and a detection zone present at another end of the device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first biomolecules immobilized on a modified porous membrane having a structure of Formula (I).
Type:
Grant
Filed:
November 20, 2014
Date of Patent:
September 12, 2023
Assignee:
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD
Inventors:
Bing Li, Brian Christopher Bales, David Roger Moore, Jason Michael Nichols, Cathryn Ellen Olsen, William Christopher Alberts, Frank John Mondello
Abstract: The present invention relates to a method for detecting a target analyte using a gold nanoparticle, comprising growing a copper crystal specifically on a gold nanoparticle by treating the gold nanoparticle with a solution comprising a copper ion, a polymer having a primary or secondary amine group, and a reducing agent, a composition for amplifying a signal used in the detection method above, and a kit for detecting a target analyte comprising the composition for amplifying a signal above.
Type:
Grant
Filed:
February 14, 2018
Date of Patent:
September 5, 2023
Assignees:
KOREA FOOD & DRUG ADMINISTRATION, SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION
Inventors:
Jwa-Min Nam, Gwangpyo Ko, Jae-Ho Kim, Jeong-Eun Park, Mouhong Lin, In Sun Joo, Jeong Su Lee
Abstract: Disclosed herein are methods and assays for the detection and/or quantification of one or more proteins or substances of interest in a sample, such as a plant, human, insect, microorganism or mammalian sample.
Type:
Grant
Filed:
September 2, 2016
Date of Patent:
August 29, 2023
Inventors:
Kristi Ruth Harkins, Aaron Joseph Walck
Abstract: Embodiments of the present disclosure provide for contrast agents, methods of making contrast agents, and methods of using contrast agents, and the like.
Type:
Grant
Filed:
October 12, 2020
Date of Patent:
August 29, 2023
Assignee:
Georgia State University Research Foundation, Inc.
Inventors:
Jenny Jie Yang, Zhiren Liu, Jie Jiang, Shenghui Xue, Jinjuan Qiao, Mani Salarian
Abstract: This invention resides in using metal complex-activated particles to bind molecules, polymers and other particles to each other, so as to produce multifunctional conjugates having controlled ratios of two or more different molecules.
Abstract: Immobilising isolated mycolic acid antigens of tuberculous mycobacterial origin or a synthetic analogue thereof on a screen-printed electrode by binding of the antigens to a self-assembled monolayer comprising a thiolated hydrophobic substance to produce immobilized mycolic acids antigens in the form of a mycolic acid antigen-containing self-assembled monolayer coating on a surface of the electrode.
Type:
Grant
Filed:
December 24, 2019
Date of Patent:
August 15, 2023
Assignee:
University of Pretoria
Inventors:
Jan Adrianus Verschoor, Carl Baumeister
Abstract: A detection reagent for comprehensively detecting an oxidized state and a glycated state of a low-density lipoprotein. While an oxidized low-density lipoprotein and a glycated low-density lipoprotein are detected with a fluolophore-labelled antibody, a lipid radical is detected with a fluorescent nitroxide 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperadine-1-oxyl (NBD-Pen).
Abstract: The present application provides stable peptide-based IL-17F and IL-17A capture agents and methods of use as detection agents. The application further provides methods of manufacturing IL-17F capture agents.
Type:
Grant
Filed:
June 15, 2018
Date of Patent:
August 8, 2023
Assignees:
INDI MOLECULAR, INC., CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventors:
Heather Agnew, Bert Tsunyin Lai, Suresh Mark Pitram, Blake Farrow, James R. Heath, David Bunck, Jingxin Liang, Arundhati Nag, Samir Das
Abstract: A method for measuring the presence of calprotectin (S100A8/A) heterodimer in a biological sample using a particle-enhanced turbidimetric immunoassay (PETIA) based on monoclonal antibodies. The method can be adapted on automated standard analyzers and provides a reliable clinical measurement of calprotectin in faecal samples and extracts. The method is comparable to commerical two-site sandwich ELISA. The disclosed method counters spontaneous agglutination caused by calcium ions and low-molecular weight calcium-binding S100 proteins as observed with conventional PETIAs. The method can be used for measuring the presence of human calprotectin in stool, urine, serum, plasma, synovial liquid and other body liquids. Metrological traceability and high commutability with conventional immunoassays (ELISA) has been shown despite of different measurement principles used.
Type:
Grant
Filed:
May 9, 2018
Date of Patent:
August 1, 2023
Assignees:
Immundiagnostik AG, DiaSys Diagnostic Systems GmbH
Inventors:
Franz-Paul Armbruster, Matthias Grimmler, Pia Schu, Tobias Becker, Felix Walzer
Abstract: Compounds and methods for use in detecting pregabalin in a sample suspected of containing pregabalin are disclosed. Pregabalin derivatives are described for producing pregabalin conjugates. A pregabalin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-pregabalin antibody. A pregabalin-detectable label conjugate may be used in a signal producing system in pregabalin assays.
Type:
Grant
Filed:
May 8, 2020
Date of Patent:
July 18, 2023
Assignee:
ARK Diagnostics, Inc.
Inventors:
Johnny Valdez, Byung Sook Moon, Alejandro A. Orozco, Gonzalo Naranjo
Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.
Type:
Grant
Filed:
September 21, 2018
Date of Patent:
July 11, 2023
Assignee:
University of Washington through its Center for Commercialization
Inventors:
Daniel T. Chiu, Changfeng Wu, Yu Rong, Yong Zhang, Yi-Che Wu, Yang-Hsiang Chan, Xuanjun Zhang, Jiangbo Yu, Wei Sun
Abstract: The present invention relates to a method of preparing a molecular sensor that is specific for a target molecule having a saccharide or peptide region. The method comprises using the target molecule as a template and incubating the template with a receptor to form a template-receptor complex. A molecular scaffold is formed on a surface around the template-receptor complex such that the receptor and at least a portion of the template are embedded in the scaffold, and the template is removed to produce a cavity defined by the scaffold, such that the cavity is complementary to at least a portion of the saccharide or peptide region of the target molecule.
Abstract: An object of the present invention is to provide a kit and a method which are capable of measuring a measurement target substance in a biological sample with high precision in a measurement range from a low concentration range to a high concentration range. According to the present invention, there is provided a kit for measuring a measurement target substance in a biological sample, including a labeled particle having a first binding substance capable of binding to a measurement target substance in a biological sample, and a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance, in which the labeled particle is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle. Each symbol in Formula (1) has the meaning described in the present specification.
Abstract: Provided herein are cell-permeable, cell-compatible, and chemoselectively-cleavable linkers for tethering (e.g., covalently) functional elements (e.g., a cellular interaction element and a capture element), and methods of use (e.g., intracellular capture and extracellular release of cellular targets) therewith.
Type:
Grant
Filed:
March 9, 2020
Date of Patent:
June 6, 2023
Assignee:
Promega Corporation
Inventors:
Sergiy Levin, Rachel Friedman Ohana, Thomas Kirkland, Keith V. Wood
Abstract: A complex comprises a CRBN having a europium-anti-his antibody on the N-terminus of the CRBN, and a Cy5-conjugated small molecule, wherein the Cy5-conjugated small molecule binds the CRBN, and uses thereof for identifying therapeutic compounds.
Type:
Grant
Filed:
September 18, 2020
Date of Patent:
May 9, 2023
Assignee:
CELGENE CORPORATION
Inventors:
Philip Paul Chamberlain, Mary Matyskiela, Godrej Khambatta
Abstract: This invention is directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins. This invention further provides a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.
Type:
Grant
Filed:
February 6, 2020
Date of Patent:
May 2, 2023
Assignee:
YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Inventors:
David Margulies, Leila Motiei, Naama Lahav