Abstract: A complex comprises a CRBN having a europium-anti-his antibody on the N-terminus of the CRBN, and a Cy5-conjugated small molecule, wherein the Cy5-conjugated small molecule binds the CRBN, and uses thereof for identifying therapeutic compounds.
Type:
Grant
Filed:
September 18, 2020
Date of Patent:
May 9, 2023
Assignee:
CELGENE CORPORATION
Inventors:
Philip Paul Chamberlain, Mary Matyskiela, Godrej Khambatta
Abstract: This invention is directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins. This invention further provides a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.
Type:
Grant
Filed:
February 6, 2020
Date of Patent:
May 2, 2023
Assignee:
YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Inventors:
David Margulies, Leila Motiei, Naama Lahav
Abstract: The present invention relates to methods to use cyclohexan-1,2-dione (CHD) groups to attach labels, linkers, and other molecules to a target compound comprising a CHD-reactive group such as a guanidine, amidine, urea, thiourea and the like. Methods of the invention include milder conditions than those previously known for promoting reaction of CHD with CHD-reactive groups, which makes the methods suitable for use with base-sensitive compounds and complex biomolecules. Methods of the invention are especially useful for attaching linking and labeling groups to a peptide that comprises at least one arginine residue, and can also be used to link such peptides to other target molecules such as nucleic acids. The invention also provides CHD-containing conjugation reagents and compositions comprising CHD-containing intermediates, and precursors useful for making CHD-containing compounds that can be used in the methods of the invention.
Type:
Grant
Filed:
November 24, 2021
Date of Patent:
May 2, 2023
Assignee:
Encodia, Inc.
Inventors:
Robert C. James, Stephen Verespy, III, Eric Cunyu Zhou, Eric Okerberg, Kelsey Rose Schramma, Fei Huang
Abstract: Methods and compositions are provided that include a multichromophore and/or multichromophore complex for identifying a target biomolecule. A sensor biomolecule, for example, an antibody can be covalently linked to the multichromophore. Additionally, a signaling chromophore can be covalently linked to the multichromophore. The arrangement is such that the signaling chromophore is capable of receiving energy from the multichromophore upon excitation of the multichromophore. Since the sensor biomolecule is capable of interacting with the target biomolecule, the multichromophore and/or multichromophore complex can provide enhanced detection signals for a target biomolecule.
Type:
Grant
Filed:
April 21, 2022
Date of Patent:
May 2, 2023
Assignee:
SIRIGEN II LIMITED
Inventors:
Brent S. Gaylord, Janice W. Hong, Tsu-Ju Fu, ChengJun Sun, Russell A. Baldocchi
Abstract: The present invention provides fluorescent compounds of formula (I) as disclosed herein or pharmaceutically acceptable salts thereof. Further provided are uses of said fluorescent compounds in methods of determining, in a sample, the presence and/or amount of pluripotent stem cells or cells undergoing reprogramming to become induced pluripotent stem cells.
Type:
Grant
Filed:
July 4, 2018
Date of Patent:
April 25, 2023
Assignee:
Agency for Science, Technology and Research
Inventors:
Sandhya Sriram, Shigeki Sugii, Young Tae Chang, Nam-Young Kang
Abstract: Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.
Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.
Type:
Grant
Filed:
February 28, 2020
Date of Patent:
April 11, 2023
Assignee:
Promega Corporation
Inventors:
Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.
Type:
Grant
Filed:
June 30, 2016
Date of Patent:
April 4, 2023
Assignee:
SHENZHEN YHLO BIOTECH CO., LTD.
Inventors:
Fuzhen Xia, Dingbiao Zou, Gang Wang, Xiaoqin Ye, Chungen Qian
Abstract: [Problem] To provide a method of scavenging an unstable radical derived from a lipid and analyzing structures of those radicals. [Means for solution] A fluorescent nitroxide (NBD-Pen) is made to act to scavenge a lipid radical or a fragment radical thereof, and fluorescent detection liquid chromatography (LC/FL) and mass spectrometry (MS) are combined to identify lipid-derived radicals contained in a lipid extract.
Abstract: A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.
Type:
Grant
Filed:
February 5, 2019
Date of Patent:
March 7, 2023
Assignee:
Clemson University Research Foundation
Inventors:
R. Kenneth Marcus, Terri F. Bruce, Lei Wang, Sisi Huang, Tyler Y. Slonecki, Rhonda Reigers Powell
Abstract: Provided are a novel immuno-optomagnetic point-of-care (PoC) assay and in particular, a method for detecting an analyte using magnetic nanoparticles and quantum dots (QD) having antibodies which are interfaced with the fabricated PoC biochip platform for quantitative analysis, and an immuno-optomagnetic detection method. The method also relates to methods of making such a plurality of conjugated magnetic quantum dot nanoparticles, methods of detecting analytes using such a plurality of conjugated quantum dot nanoparticles.
Type:
Grant
Filed:
February 17, 2020
Date of Patent:
March 7, 2023
Assignee:
SABANCI UNIVERSITESI NANOTEKNOLOJI ARASTIRMA VE UYGULAMA MERKEZI
Abstract: Methods and reagents are disclosed for minimizing a false result in an assay measurement for determining a concentration of an analyte in a sample suspected of containing the analyte. The method comprises pretreating both an antibody and a sample to be subjected to a non-agglutination immunoassay. In the method the antibody and the sample are combined with a pretreatment agent selected from the group consisting of hydroxyphenyl-substituted C1-C5 carboxylic acids and metallic salts thereof and halogen-substituted C1-C5 carboxylic acids and metallic salts thereof in an amount effective to enhance the accuracy of the non-agglutination immunoassay.
Abstract: The invention provides a method that gives direct information about the intervention of a potential drug on the secondary structure distribution of a targetbiomolecule, i.e., for a disease with misfolded protein, such as neurodegenerative diseases in a complex body fluid. The secondary structural change is monitored by vibrational spectroscopy. The method can be applied for prescreening of drug candidates for targeting of specific biomolecules. The effect of the drug on the secondary structure distribution is monitored label-free in real time and provides thereby direct information about the efficacy of the potential drug.
Type:
Grant
Filed:
November 21, 2017
Date of Patent:
February 28, 2023
Assignee:
betaSENSE GmbH
Inventors:
Klaus Gerwert, Andreas Nabers, Jonas Schartner
Abstract: The present disclosure provides fluorescent polyindenofluorene polymers or macromers with unique optical properties that are stable. The polymeric fluorophores are useful in various bioassays formats. The inventive polymers are useful in assays relying on fluorescence resonance energy transfer (FRET) mechanisms where two fluorophores are used.
Abstract: The present disclosure provides isolated antibodies that bind to acetaminophen-protein adducts that are useful in the detection and diagnosis of acetaminophen-induced toxicity.
Type:
Grant
Filed:
January 10, 2020
Date of Patent:
January 31, 2023
Assignees:
BioVentures, LLC, Arkansas Children's Research Institute
Inventors:
Dean W. Roberts, Laura James, Jack Hinson
Abstract: The present disclosure provides a method for detecting cholangiocarcinoma cells. The capture rate of the cholangiocarcinoma cells of the present disclosure is higher than 70%, and a plurality of octasaccharides with high affinity and specificity can be modified on the surface of magnetic beads to capture and analyze cholangiocarcinoma cells under test, wherein the cholangiocarcinoma cells can be circulating tumor cells in cholangiocarcinoma.
Type:
Grant
Filed:
July 19, 2019
Date of Patent:
January 10, 2023
Assignees:
NATIONAL TSING HUA UNIVERSITY, ACADEMIA SINICA
Inventors:
Gwo Bin Lee, Shang-Cheng Hung, Wei-Chun Tsai
Abstract: Disclosed herein are methods for identifying proteins as targets for interaction with a small molecule ligand. Also disclosed herein are small molecule ligands and compositions for use in profiling druggable proteins.
Type:
Grant
Filed:
January 17, 2018
Date of Patent:
December 27, 2022
Assignee:
The Scripps Research Institute
Inventors:
Benjamin F. Cravatt, Christopher G. Parker, Bruno Correia
Abstract: Compounds and methods for use in detecting gabapentin in a sample suspected of containing gabapentin are disclosed. Gabapentin derivatives are used to produce gabapentin conjugates. A gabapentin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-gabapentin antibody. A gabapentin-detectable label may be used in a signal producing system in gabapentin assays.
Type:
Grant
Filed:
June 22, 2022
Date of Patent:
December 13, 2022
Assignee:
ARK Diagnostics, Inc.
Inventors:
Johnny Jose Valdez, Byung Sook Moon, Michael Kevin Helms, Alejandro A. Orozco